Green synthesis of copper oxide nanoparticles using Cedrus deodara aqueous extract for antibacterial activity

Author(s):  
M. Ramzan ◽  
Raphael.M. Obodo ◽  
S. Mukhtar ◽  
S.Z. Ilyas ◽  
Fahad Aziz ◽  
...  
Processes ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 78
Author(s):  
Karla Araya-Castro ◽  
Tzu-Chiao Chao ◽  
Benjamín Durán-Vinet ◽  
Carla Cisternas ◽  
Gustavo Ciudad ◽  
...  

Amongst different living organisms studied as potential candidates for the green synthesis of copper nanoparticles, algal biomass is presented as a novel and easy-to-handle method. However, the role of specific biomolecules and their contribution as reductant and capping agents has not yet been described. This contribution reports a green synthesis method to obtain copper oxide nanoparticles (CuO-NPs) using separated protein fractions from an aqueous extract of brown algae Macrocystis pyrifera through size exclusion chromatography (HPLC-SEC). Proteins were detected by a UV/VIS diode array, time-based fraction collection was carried out, and each collected fraction was used to evaluate the synthesis of CuO-NPs. The characterization of CuO-NPs was evaluated by Dynamic Light Scattering (DLS), Z-potential, Fourier Transform Infrared (FTIR), Transmission Electron Microscope (TEM) equipped with Energy Dispersive X-ray Spectroscopy (EDS) detector. Low Molecular Weight (LMW) and High Molecular Weight (HMW) protein fractions were able to synthesize spherical CuO-NPs. TEM images showed that the metallic core present in the observed samples ranged from 2 to 50 nm in diameter, with spherical nanostructures present in all containing protein samples. FTIR measurements showed functional groups from proteins having a pivotal role in the reduction and stabilization of the nanoparticles. The highly negative zeta potential average values from obtained nanoparticles suggest high stability, expanding the range of possible applications. This facile and novel protein-assisted method for the green synthesis of CuO-NPs may also provide a suitable tool to synthesize other nanoparticles that have different application areas.


2018 ◽  
Vol 29 (12) ◽  
pp. 3315-3326 ◽  
Author(s):  
Shanmugam Prakash ◽  
Nagaraj Elavarasan ◽  
Alagesan Venkatesan ◽  
Kasivisvanathan Subashini ◽  
Murugesan Sowndharya ◽  
...  

2021 ◽  
Author(s):  
CI Chemistry International

The aim of present study is based on low cost, efficient, non-toxic and eco-friendly method for green synthesis of copper oxide nanoparticles (CuONPs) using Bougainvillea leaves aqueous extract. The green synthesized nanoparticles were subjected to characterization techniques UV–visible spectroscopy (UV–vis), X-ray diffraction (XRD), Fourier transform Infrared spectroscopy (FT-IR) and transmission electron microscope (TEM). The synthesized CuONPs were pure, predominantly spherical with sizes ranges from 8-20 nm. CuONPs showed excellent antimicrobial activity against various bacterial strains Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus. Moreover, E. coli and E. faecalis exhibited the highest sensitivity to CuONPs while Staphylococcus aureus was the least sensitive. Possible mechanisms of antimicrobial activity of CuONPs should be further investigated.


2020 ◽  
Vol 09 ◽  
Author(s):  
Akshaya Venkatramanan ◽  
Akila Ilangovan ◽  
Pakutharivu Thangarajan ◽  
Anitha Saravanan ◽  
Balachandar M

Background: The Nanomaterials/Nanoparticles are of great interest today because of their small size and large surface area, modular and easily tunable morphology and size. Copper oxide (CuO) nanoparticles are widely used in dye-sensitized solar cells (DSSCs). Research on the synthesis and properties of metallic nanomaterials is a growing field of nanotechnology due to the use of nanoparticles in the scientific, technical, pharmaceutical, and biomedical fields. Green synthesis is an emerging technology for the production of nanoparticles due to its many advantages over traditional physical processes and the method of chemical synthesis. Methods: In this study, we report the cost-effective, long-lasting, stable, and regenerative aqueous extract of Eletteria cardamom seeds to target the synthesis of copper oxide nanoparticles (CuO NPs). This method is completely green, free from toxic and harmful solvents. CuO NPs were synthesized from a cupric nitrate mixture and the aqueous extracts of Eletteria cardamom seeds were kept at room temperature for 24 h. CuO NPs were characterized using UV-visible spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), dynamic light scattering (DLS), and Fourier Transfer infra-red spectroscopy (FTIR) analyzes. UV - Vis spectroscopy revealed the presence of CuO NPs. Results: SEM images stated that the particles were spherical and ranged in size from 1–100nm. FTIR spectra of control (seed extract) and synthesized CuO NPs identify functional groups of active components. In addition, the synthesized CuO NPs were tested for antimicrobial activity by standard disc diffusion method. Conclusion: Nanoparticles found that Escherichia coli and Staphylococcus aureus resistant areas were observed around each well with antimicrobial activity against disease-causing pathogenic strains.


Author(s):  
Haider Qassim Raheem ◽  
Takwa S. Al-meamar ◽  
Anas M. Almamoori

Fifty specimens were collected from wound patients who visited Al-Hilla Teaching Hospital. The samples were grown on Blood and MacConkey agar for 24-48 hr at 37oC. The bacterial isolates which achieved as a pure and predominant growth from clinical samples as Pseudomonas fluorescens, were identified using morphological properties and Vitek2 system. The anti-bacterial activity of copper oxide nanoparticles (CuO NPs) against was tested by (disk diffusion assay) using dilutions of (400, 200, 100, 50, 25, and 12.5‎µ‎g/ml). The (MIC and MBC) of each isolate was determined. CuO NPs shows wide spectrum antibacterial activity against tested bacteria with rise zone of inhibition diameter that is proportionate with the increase in nanoparticle concentration. The MIC of CuO NPs extended from 100-200‎µ‎g/ml and the MBC ranged from 200-400‎µ‎g/ml. The antibiotic profile was determined by Viteck 2 compact system (Biomérieux). CuO NPs‎ found highly effective and safe in P. fluorescens wounds infections comparing with used antibiotics.


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