Initial adhesion suppression of biofilm-forming and copper-tolerant bacterium Variovorax sp. on laser microtextured copper surfaces

2021 ◽  
Vol 202 ◽  
pp. 111656
Author(s):  
Luis Caro-Lara ◽  
Esteban Ramos-Moore ◽  
Ignacio T. Vargas ◽  
Magdalena Walczak ◽  
Christian Fuentes ◽  
...  
Author(s):  
W. A. Chiou ◽  
N. Kohyama ◽  
B. Little ◽  
P. Wagner ◽  
M. Meshii

The corrosion of copper and copper alloys in a marine environment is of great concern because of their widespread use in heat exchangers and steam condensers in which natural seawater is the coolant. It has become increasingly evident that microorganisms play an important role in the corrosion of a number of metals and alloys under a variety of environments. For the past 15 years the use of SEM has proven to be useful in studying biofilms and spatial relationships between bacteria and localized corrosion of metals. Little information, however, has been obtained using TEM capitalizing on its higher spacial resolution and the transmission observation of interfaces. The research presented herein is the first step of this new approach in studying the corrosion with biological influence in pure copper.Commercially produced copper (Cu, 99%) foils of approximately 120 μm thick exposed to a copper-tolerant marine bacterium, Oceanospirillum, and an abiotic culture medium were subsampled (1 cm × 1 cm) for this study along with unexposed control samples.


1986 ◽  
Vol 47 (C8) ◽  
pp. C8-487-C8-490
Author(s):  
M. D. CRAPPER ◽  
C. E. RILEY ◽  
D. P. WOODRUFF

1986 ◽  
Vol 21 (4) ◽  
pp. 486-495 ◽  
Author(s):  
R.F.G. Selle Sardi ◽  
W. Bulani ◽  
W.L. Cairns ◽  
N. Kosaric

Abstract Ion exchanger beads are explored as aids in accelerating the development of anaerobic biofilms for use in advanced anaerobic reactors. Initial adhesion and subsequent changes in adhesion and growth of anaerobic biofilms (as reflected in total supported biomass and metabolic activity) were monitored on different ion exchangers (strong cation, strong anion and weak anion) over a period of 12 days. Metabolic activity was recorded for the first time in this type of study using ATP biolumininescence assays which allowed monitoring of rapid changes in the biofilm development. Results indicate that the strong cation exchanger is a better overall substratum for anaerobic biofilm development due to its favorable property of dialent cation binding and adsorption of volatile fatty acid substrate for methanogens.


1975 ◽  
Vol 29 (6) ◽  
pp. 496-500 ◽  
Author(s):  
D. Kember ◽  
N. Sheppard

Infrared emission spectra from metal samples with oxide surface layers are shown to be very advantageously studied using the spectrum-ratioing facility of a recording infrared interferometer. The emission from a given sample is ratioed against that from a black-body emitter at the same temperature so as to give emittance as a function of wavenumber directly. This method has very useful application to irregularly shaped metal emitters. In the absence of selective reflection there is a direct correspondence between emission and absorption spectra for thin layers of an emitting substance. However, the presence of selective reflection leads to reduced emission and to considerable differences in the appearance of “absorption” and emission spectra in regions of strong absorption. Emission spectra obtained from copper plates heated, above 150°C, for different periods in air are shown clearly to indicate the presence of cuprous, Cu(I), and cupric, Cu(II), oxides in the surface layer.


2021 ◽  
Vol 194 ◽  
pp. 116925
Author(s):  
Jinfeng Wang ◽  
Qiuju Liu ◽  
Deyuan Dong ◽  
Haidong Hu ◽  
Bing Wu ◽  
...  

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2247
Author(s):  
Fernando da Silva Souza ◽  
José Maria Franco de Carvalho ◽  
Gabriela Grotti Silveira ◽  
Vitória Cordeiro Araújo ◽  
Ricardo André Fiorotti Peixoto

The lack of usable aggregates for civil construction in Rio Branco (capital of Acre, a Federal State in the Amazon region) makes the production and use of recycled aggregates from construction and demolition waste (CDW) an alternative of great interest. In this study, a comprehensive characterization of CDW collected from 24 construction sites of six building types and three different construction phases (structures, masonry, and finishing) was carried out. The fine and coarse recycled aggregates were produced and evaluated in 10 different compositions. The aggregates’ performance was evaluated in four mixtures designed for laying and coating mortars with a total replacement of conventional aggregates and a mixture designed for a C25 concrete with 50% and 100% replacement of conventional aggregates. CDW mortars showed lower densities and greater water retention, initial adhesion, and mechanical strength than conventional mortars. CDW concretes presented lower densities and greater resistance to chloride penetration than conventional concrete, with a small mechanical strength reduction. The recycled CDW aggregates proved to be technologically feasible for safe application in mortars and concrete; for this reason, it is believed that the alternative and proposed methodology is of great interest to the Amazonian construction industry, considering the high costs of raw materials and the need for defining and consolidating a sustainable development model for the Amazon region.


2008 ◽  
Vol 74 (17) ◽  
pp. 5511-5515 ◽  
Author(s):  
Henny C. van der Mei ◽  
Minie Rustema-Abbing ◽  
Joop de Vries ◽  
Henk J. Busscher

ABSTRACT Transition from reversible to irreversible bacterial adhesion is a highly relevant but poorly understood step in initial biofilm formation. We hypothesize that in oral biofilm formation, irreversible adhesion is caused by bond strengthening due to specific bacterial interactions with salivary conditioning films. Here, we compared the initial adhesion of six oral bacterial strains to salivary conditioning films with their adhesion to a bovine serum albumin (BSA) coating and related their adhesion to the strengthening of the binding forces measured with bacteria-coated atomic force microscopy cantilevers. All strains adhered in higher numbers to salivary conditioning films than to BSA coatings, and specific bacterial interactions with salivary conditioning films were accompanied by stronger initial adhesion forces. Bond strengthening occurred on a time scale of several tens of seconds and was slower for actinomyces than for streptococci. Nonspecific interactions between bacteria and BSA coatings strengthened twofold faster than their specific interactions with salivary conditioning films, likely because specific interactions require a closer approach of interacting surfaces with the removal of interfacial water and a more extensive rearrangement of surface structures. After bond strengthening, bacterial adhesion forces with a salivary conditioning film remained stronger than those with BSA coatings.


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