Farnesylation and lipid unsaturation are critical for the membrane binding of the C-terminal segment of G-protein receptor kinase 1

2022 ◽  
pp. 112315
Author(s):  
Marc-Antoine Millette ◽  
Sarah Roy ◽  
Christian Salesse
Keyword(s):  
G Protein ◽  
Membrane Binding ◽  
Terminal Segment ◽  
Receptor Kinase ◽  
Protein Receptor ◽  
Lipid Unsaturation

AGONIST ACTIVATED ADRENOCORTICOTROPIN RECEPTOR INTERNALIZES VIA A CLATHRIN-MEDIATED G PROTEIN RECEPTOR KINASE DEPENDENT MECHANISM

2002 ◽  
Vol 28 (4) ◽  
pp. 281-289 ◽  
Author(s):  
A. H. Baig ◽  
F. M. Swords ◽  
M. Szaszák ◽  
P. J. King ◽  
L. Hunyady ◽  
...  
Keyword(s):  
G Protein ◽  
Receptor Kinase ◽  
Protein Receptor ◽  
Dependent Mechanism

Effect of Paroxetine-Mediated G-Protein Receptor Kinase 2 Inhibition vs Placebo in Patients With Anterior Myocardial Infarction

2021 ◽  
Author(s):  
Thomas Pilgrim ◽  
René Vollenbroich ◽  
Sarah Deckarm ◽  
Christoph Gräni ◽  
Stephan Dobner ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
G Protein ◽  
Receptor Kinase ◽  
Anterior Myocardial Infarction ◽  
Protein Receptor

scholarly journals Opsin 3–Gαs Promotes Airway Smooth Muscle Relaxation Modulated by G Protein Receptor Kinase 2

2021 ◽  
Vol 64 (1) ◽  
pp. 59-68
Author(s):  
Amy D. Wu ◽  
William Dan ◽  
Yi Zhang ◽  
Shruti Vemaraju ◽  
Brian A. Upton ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
G Protein ◽  
Airway Smooth Muscle ◽  
Muscle Relaxation ◽  
Smooth Muscle Relaxation ◽  
Receptor Kinase ◽  
Protein Receptor

Differential G protein receptor kinase 2 expression in compensated hypertrophy and heart failure after myocardial infarction in the rat

2003 ◽  
Vol 98 (2) ◽  
pp. 97-103 ◽  
Author(s):  
J. Theilade ◽  
C. Str�m ◽  
T. Christiansen ◽  
S. Hauns� ◽  
S. P. Sheikh
Keyword(s):  
Myocardial Infarction ◽  
Heart Failure ◽  
G Protein ◽  
Receptor Kinase ◽  
Protein Receptor

scholarly journals Unveiling the Membrane-Binding Properties of N-Terminal and C-Terminal Regions of G Protein-Coupled Receptor Kinase 5 by Combined Optical Spectroscopies

Langmuir ◽  
2014 ◽  
Vol 30 (3) ◽  
pp. 823-831 ◽  
Author(s):  
Bei Ding ◽  
Alisa Glukhova ◽  
Katarzyna Sobczyk-Kojiro ◽  
Henry I. Mosberg ◽  
John J. G. Tesmer ◽  
...  
Keyword(s):  
G Protein ◽  
Membrane Binding ◽  
Receptor Kinase ◽  
G Protein Coupled Receptor ◽  
Binding Properties ◽  
G Protein Coupled

Phosphodiesterase-4 gates the ability of protein kinase A to phosphorylate G-protein receptor kinase-2 and influence its translocation

2006 ◽  
Vol 34 (4) ◽  
pp. 474-475 ◽  
Author(s):  
M.D. Houslay ◽  
G.S. Baillie
Keyword(s):  
Protein Kinase ◽  
G Protein ◽  
Fluorescent Protein ◽  
Resting Cells ◽  
Receptor Kinase ◽  
Human Embryonic Kidney Cells ◽  
Phosphodiesterase 4 ◽  
Protein Receptor ◽  
Dependent Protein ◽  
Green Fluorescent

Challenge of the β2Ar (β2-adrenergic receptor) with isoprenaline in HEK-293β2 cells (human embryonic kidney cells stably overexpressing a FLAG- and green fluorescent protein-tagged β2Ar) results in the PKA (cAMP-dependent protein kinase) phosphorylation of GRK2 (G-protein receptor kinase-2). This response was enhanced when PDE4 (phosphodiesterase-4) activity was attenuated using either rolipram, a PDE4-selective inhibitor, or with siRNA (small interfering RNA) knockdown of both PDE4B and PDE4D. Rolipram also facilitated GRK2 recruitment to the membrane and phosphorylation of the β2Ar by GRK2 in response to isoprenaline challenge of cells. In resting cells, rolipram treatment alone is sufficient to promote PKA phosphorylation of GRK2, with consequential effects on GRK2 translocation and GRK2 phosphorylation of the β2Ar. Similar effects are observed in cardiac myocytes. We propose that PDE4 activity protects GRK2 from inappropriate phosphorylation by PKA in resting cells that might have occurred through fluctuations in basal cAMP levels. Thus PDE4 gates the action of PKA to phosphorylate GRK2.

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