scholarly journals Detoxification of sunset yellow and brilliant blue dyes using soybean peroxidases

2021 ◽  
pp. 100215
Author(s):  
Shweta Sharma ◽  
Nithya Rani R ◽  
Vadiraj K.T. ◽  
Ravi MB ◽  
Chandan S ◽  
...  
Keyword(s):  
Brilliant Blue ◽  
Sunset Yellow

Green effervescence assisted dispersive liquid–liquid microextraction based on a hydrophobic deep eutectic solvent for determination of Sunset Yellow and Brilliant Blue FCF in food samples

2018 ◽  
Vol 42 (18) ◽  
pp. 14901-14908 ◽  
Author(s):  
Mahboobeh Ghorbani Ravandi ◽  
Mohammad Reza Fat’hi
Keyword(s):  
Deep Eutectic Solvent ◽  
Food Samples ◽  
Brilliant Blue ◽  
Synthetic Dyes ◽  
Sunset Yellow ◽  
Brilliant Blue Fcf ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction ◽  
Sensitive Method

In this study, a simple, fast and sensitive method called effervescence assisted dispersive liquid–liquid microextraction based on a hydrophobic deep eutectic solvent (EADLLME-DES) was used to extract synthetic dyes from food samples.

Simultaneous Preconcentration and Determination of Brilliant Blue and Sunset Yellow in Foodstuffs by Solid-Phase Extraction Combined UV-Vis Spectrophotometry

2018 ◽  
Vol 101 (6) ◽  
pp. 1850-1856 ◽  
Author(s):  
Abdullah Taner Bişgin
Keyword(s):  
Solid Phase Extraction ◽  
Flow Rate ◽  
Solid Phase ◽  
Ethanol Solution ◽  
Water Soluble ◽  
Brilliant Blue ◽  
Phase Extraction ◽  
Sunset Yellow ◽  
Food Dyes

Abstract Background: Brilliant Blue and Sunset Yellow, two highly water-soluble synthetic food dyes, are the most popular food dyes used and consumed. Although they are not highly toxic, some health problems can be observed when excessive amounts of food products containing these dyes are consumed. Objectives: The aim of the study was to develop a simultaneous UV-Vis combined solid-phase extraction method, based on the adsorption onto Amberlite XAD-8 resin, for determination of Brilliant Blue and Sunset Yellow dyes. Methods: Sample solution was poured into the reservoir of the column and permitted to gravitationally pass through the column at 2 mL/min flow rate. Adsorbed dyes were eluted to 5 mL of final volume with 1 mol/L HNO3 in ethanol solution by applying a 2 mL/min flow rate. Dye concentrations of the solution were determined at 483 and 630 nm for Sunset Yellow and Brilliant Blue, respectively. Results: The detection limits of the method for Brilliant Blue and Sunset Yellow were determined as 0.13 and 0.66 ng/mL, respectively. Preconcentration factor was 80. Brilliant Blue contents of real food samples were found to be between 11 and 240 μg/g. Sunset Yellow concentrations of foodstuffs were determined to be between 19 and 331 μg/g. Conclusions: Economical, effective, and simple simultaneous determination of Brilliant Blue and Sunset Yellow was achieved by using a solid-phase extraction combined UV-Vis spectrometry method. Highlights: The method is applicable and suitable for routine analysis in quality control laboratories without the need for expert personnel and high operational costs because the instrumentation is simple and inexpensive.

scholarly journals Food dyes as an alternative tracking dye for DNA gel electrophoresis

2013 ◽  
Vol 10 (4) ◽  
pp. 1150-1156 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Uv Light ◽  
Brilliant Blue ◽  
Local Market ◽  
Synthetic Dyes ◽  
Sunset Yellow ◽  
Dna Ladder ◽  
Food Dye ◽  
Brilliant Blue Fcf ◽  
Wide Range ◽  
Food Dyes

The chemical, physical and toxicological effects on health of synthetic dyes that used as tracking dye in the electrophoresis requires seriously search about alternative tracking dye. The present study is aimed to find an alternative dye from safe food dyes which commonly used in food coloring. Five dyes were selected depending on their chemical properties and the availability in local market: Brilliant Blue FCF, Tartrazine, Sunset Yellow FCF, Carmoisine, and green traditional, three dyes were chosen to be mixed as loading buffer: Brilliant Blue FCF, Sunset Yellow FCF as a basic because it give the whole range size of most traditional loading buffers that available in market, and adding the Carmoisine as a new indicator for the bands less than 50bp, then mixed with DNA ladder in same percentage used with traditional loading buffers to clarify the effects of dyes on DNA, migrated on 1% agarose with loading buffer promega, results showed more clarity and highly readable separation of dyes and give wide range of size in the food loading mix than promega loading dye, by viewing the gel on UV light the DNA ladder were moved smoothly, bands separated effeminately on gel and in same rate of the DNA ladder that load with promega loading buffer which indicate no interaction between the food dyes and the DNA.Our studies show that the food dye can be used as a tracking dye in place of used synthetic dye. The procedure is found to be easy, practical, safely and reliable.

Level of food additives, caffeine and total sugars in locally manufactured beverages in Mauritius and their compliance with national and international norms

2019 ◽  
Vol 121 (11) ◽  
pp. 2849-2866
Author(s):  
Dhamawatee Harnarun Etwaroo ◽  
Viswen Armoogum ◽  
Dayawatee Goburdhun ◽  
Arvind Ruggoo ◽  
Pooja Dookheea ◽  
...  
Keyword(s):  
Citric Acid ◽  
Sorbic Acid ◽  
Food Additives ◽  
Soft Drinks ◽  
International Norms ◽  
Brilliant Blue ◽  
Sunset Yellow ◽  
Total Sugars ◽  
Content Type ◽  
Validation Parameters

Purpose The purpose of this paper is to determine the level of food additives, caffeine and total sugars in locally manufactured beverages in Mauritius and ascertain their compliance with national and international norms. Design/methodology/approach In total, 48 beverages: 21 soft drinks, 14 ice teas, 5 fruit drinks, 5 nonalcoholic sparkling drinks and 3 tonic waters were analysed for the level of sweeteners (aspartame, acesulfame K and saccharin), preservatives (benzoic and sorbic acid), colours (tartrazine, sunset yellow, quinoline yellow, allura red, amaranth, ponceau 4R, carmoisine, erythrosine, brilliant blue, green S and patent blue), citric acid, caffeine and total sugars. High-performance liquid chromatography methods used to quantify the levels of total sugars, caffeine and additives were validated against parameters such as linearity, recovery, accuracy, precision and limit of quantification. Findings Out of 48 beverages, 13 contained at least one sweetener. The most frequently used sweeteners were acesulfame K and aspartame. Benzoic acid was present in 27 samples (42.32–168.03 mg/L). Sorbic acid was present in 14 beverages (13.01–180.38 mg/L). Citric acid (0.7–4 g/L) was present in all the 48 beverages, while caffeine was present in 20 samples in the range of 14.01–129.42 mg/L. Nine samples contained at least one artificial colour and the most frequently used colours were tartrazine, sunset yellow, brilliant blue and carmoisine. The average level of total sugars present in the beverages was 10 g/100 ml. The validation parameters obtained showed evidence for method suitability. Research limitations/implications Beverages sold by individuals on the street, small restaurants and markets were not analysed. Originality/value This study provides an overview of the chemical composition of soft drinks and their compliance with Food Regulations. It also paves the way to investigate weaknesses, knowledge, attitudes and practices of local manufacturers, which leads to non-adherence to Regulations regarding food additives.

scholarly journals Environmental Detection of Brilliant Blue, Sunset Yellow and Tartrazine Using Direct Injection HPLC-DAD Technique

2020 ◽  
Vol 71 (6) ◽  
pp. 390-400
Author(s):  
Florinela Pirvu ◽  
Vasile Ion Iancu ◽  
Marcela Niculescu ◽  
Carol Blaziu Lehr ◽  
Luoana Florentina Pascu ◽  
...  
Keyword(s):  
Direct Injection ◽  
Simultaneous Detection ◽  
Correlation Coefficients ◽  
Brilliant Blue ◽  
Synthetic Dyes ◽  
Linear Calibration ◽  
Sunset Yellow ◽  
Limit Values ◽  
Wastewater Samples ◽  
Method Accuracy

A newly developed direct injection HPLC-DAD method for the simultaneous detection of Brilliant Blue (BB), Sunset Yellow (SY) and Tartrazine (TA) synthetic dyes was validated and applied to surface and wastewater samples. Chromatographic separation was achieved using an Acclaim Surfactant Plus column (150 x 3.0 mm, 3 um) in isocratic elution mode with a mobile phase consisting of 100 mM CH3COONH4 and ACN in the ratio 50/50 (v/v). Linear calibration curves were plotted in the range of 2-100 μg/L (BB) and 6-300 μg/L (SY, TA) respectively with correlation coefficients (R2) higher than 0.99. Low quantitation limit values (LOQ) were obtained for the direct injection HPLC-DAD method, namely: 1.0 �g/L (BB), 5.5 �g/L (SY) and 5.8 �g/L (TA), respectively. The entire sample preparation procedure consisted only in simple filtration using PTFE syringe filters (0.45 �m) without sample extraction or concentration, which improved method accuracy and precision. HPLC-DAD developed method proved to be fast (6 minutes run-time), sensitive and accurate and was succesfully applied for the analysis of the synthetic dyes in surface and wastewater samples. Considering all analyzed samples (8 surface water and 11 wastewater samples), Brilliant Blue was found in influent samples with values between 4.49 � 14.17 �g/L and effluent samples with values between 1.07 � 9.54 �g/L, whereas Sunset Yellow and Tartrazine were not detected in any of the analyzed samples.

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