scholarly journals All aspect of toxic effect of brilliant blue and sunset yellow in Allium cepa roots

2018 ◽  
Vol 70 (1) ◽  
pp. 449-463 ◽  
Author(s):  
Kemal Koç ◽  
Dilek Pandir
Keyword(s):  
Toxic Effect ◽  
Allium Cepa ◽  
Brilliant Blue ◽  
Sunset Yellow

Green effervescence assisted dispersive liquid–liquid microextraction based on a hydrophobic deep eutectic solvent for determination of Sunset Yellow and Brilliant Blue FCF in food samples

2018 ◽  
Vol 42 (18) ◽  
pp. 14901-14908 ◽  
Author(s):  
Mahboobeh Ghorbani Ravandi ◽  
Mohammad Reza Fat’hi
Keyword(s):  
Deep Eutectic Solvent ◽  
Food Samples ◽  
Brilliant Blue ◽  
Synthetic Dyes ◽  
Sunset Yellow ◽  
Brilliant Blue Fcf ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction ◽  
Sensitive Method

In this study, a simple, fast and sensitive method called effervescence assisted dispersive liquid–liquid microextraction based on a hydrophobic deep eutectic solvent (EADLLME-DES) was used to extract synthetic dyes from food samples.

Simultaneous Preconcentration and Determination of Brilliant Blue and Sunset Yellow in Foodstuffs by Solid-Phase Extraction Combined UV-Vis Spectrophotometry

2018 ◽  
Vol 101 (6) ◽  
pp. 1850-1856 ◽  
Author(s):  
Abdullah Taner Bişgin
Keyword(s):  
Solid Phase Extraction ◽  
Flow Rate ◽  
Solid Phase ◽  
Ethanol Solution ◽  
Water Soluble ◽  
Brilliant Blue ◽  
Phase Extraction ◽  
Sunset Yellow ◽  
Food Dyes

Abstract Background: Brilliant Blue and Sunset Yellow, two highly water-soluble synthetic food dyes, are the most popular food dyes used and consumed. Although they are not highly toxic, some health problems can be observed when excessive amounts of food products containing these dyes are consumed. Objectives: The aim of the study was to develop a simultaneous UV-Vis combined solid-phase extraction method, based on the adsorption onto Amberlite XAD-8 resin, for determination of Brilliant Blue and Sunset Yellow dyes. Methods: Sample solution was poured into the reservoir of the column and permitted to gravitationally pass through the column at 2 mL/min flow rate. Adsorbed dyes were eluted to 5 mL of final volume with 1 mol/L HNO3 in ethanol solution by applying a 2 mL/min flow rate. Dye concentrations of the solution were determined at 483 and 630 nm for Sunset Yellow and Brilliant Blue, respectively. Results: The detection limits of the method for Brilliant Blue and Sunset Yellow were determined as 0.13 and 0.66 ng/mL, respectively. Preconcentration factor was 80. Brilliant Blue contents of real food samples were found to be between 11 and 240 μg/g. Sunset Yellow concentrations of foodstuffs were determined to be between 19 and 331 μg/g. Conclusions: Economical, effective, and simple simultaneous determination of Brilliant Blue and Sunset Yellow was achieved by using a solid-phase extraction combined UV-Vis spectrometry method. Highlights: The method is applicable and suitable for routine analysis in quality control laboratories without the need for expert personnel and high operational costs because the instrumentation is simple and inexpensive.

scholarly journals Food dyes as an alternative tracking dye for DNA gel electrophoresis

2013 ◽  
Vol 10 (4) ◽  
pp. 1150-1156 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Uv Light ◽  
Brilliant Blue ◽  
Local Market ◽  
Synthetic Dyes ◽  
Sunset Yellow ◽  
Dna Ladder ◽  
Food Dye ◽  
Brilliant Blue Fcf ◽  
Wide Range ◽  
Food Dyes

The chemical, physical and toxicological effects on health of synthetic dyes that used as tracking dye in the electrophoresis requires seriously search about alternative tracking dye. The present study is aimed to find an alternative dye from safe food dyes which commonly used in food coloring. Five dyes were selected depending on their chemical properties and the availability in local market: Brilliant Blue FCF, Tartrazine, Sunset Yellow FCF, Carmoisine, and green traditional, three dyes were chosen to be mixed as loading buffer: Brilliant Blue FCF, Sunset Yellow FCF as a basic because it give the whole range size of most traditional loading buffers that available in market, and adding the Carmoisine as a new indicator for the bands less than 50bp, then mixed with DNA ladder in same percentage used with traditional loading buffers to clarify the effects of dyes on DNA, migrated on 1% agarose with loading buffer promega, results showed more clarity and highly readable separation of dyes and give wide range of size in the food loading mix than promega loading dye, by viewing the gel on UV light the DNA ladder were moved smoothly, bands separated effeminately on gel and in same rate of the DNA ladder that load with promega loading buffer which indicate no interaction between the food dyes and the DNA.Our studies show that the food dye can be used as a tracking dye in place of used synthetic dye. The procedure is found to be easy, practical, safely and reliable.

Level of food additives, caffeine and total sugars in locally manufactured beverages in Mauritius and their compliance with national and international norms

2019 ◽  
Vol 121 (11) ◽  
pp. 2849-2866
Author(s):  
Dhamawatee Harnarun Etwaroo ◽  
Viswen Armoogum ◽  
Dayawatee Goburdhun ◽  
Arvind Ruggoo ◽  
Pooja Dookheea ◽  
...  
Keyword(s):  
Citric Acid ◽  
Sorbic Acid ◽  
Food Additives ◽  
Soft Drinks ◽  
International Norms ◽  
Brilliant Blue ◽  
Sunset Yellow ◽  
Total Sugars ◽  
Content Type ◽  
Validation Parameters

Purpose The purpose of this paper is to determine the level of food additives, caffeine and total sugars in locally manufactured beverages in Mauritius and ascertain their compliance with national and international norms. Design/methodology/approach In total, 48 beverages: 21 soft drinks, 14 ice teas, 5 fruit drinks, 5 nonalcoholic sparkling drinks and 3 tonic waters were analysed for the level of sweeteners (aspartame, acesulfame K and saccharin), preservatives (benzoic and sorbic acid), colours (tartrazine, sunset yellow, quinoline yellow, allura red, amaranth, ponceau 4R, carmoisine, erythrosine, brilliant blue, green S and patent blue), citric acid, caffeine and total sugars. High-performance liquid chromatography methods used to quantify the levels of total sugars, caffeine and additives were validated against parameters such as linearity, recovery, accuracy, precision and limit of quantification. Findings Out of 48 beverages, 13 contained at least one sweetener. The most frequently used sweeteners were acesulfame K and aspartame. Benzoic acid was present in 27 samples (42.32–168.03 mg/L). Sorbic acid was present in 14 beverages (13.01–180.38 mg/L). Citric acid (0.7–4 g/L) was present in all the 48 beverages, while caffeine was present in 20 samples in the range of 14.01–129.42 mg/L. Nine samples contained at least one artificial colour and the most frequently used colours were tartrazine, sunset yellow, brilliant blue and carmoisine. The average level of total sugars present in the beverages was 10 g/100 ml. The validation parameters obtained showed evidence for method suitability. Research limitations/implications Beverages sold by individuals on the street, small restaurants and markets were not analysed. Originality/value This study provides an overview of the chemical composition of soft drinks and their compliance with Food Regulations. It also paves the way to investigate weaknesses, knowledge, attitudes and practices of local manufacturers, which leads to non-adherence to Regulations regarding food additives.

scholarly journals Citotoxicity of food dyes sunset yellow (E-110), bordeaux red (E-123), and tatrazine yellow (E-102) on Allium cepa L. root meristematic cells

2013 ◽  
Vol 33 (1) ◽  
pp. 218-223 ◽  
Author(s):  
Keiva Maria Silva Gomes ◽  
Maria Virna Gonçalves Aguiar de Oliveira ◽  
Francisco Ronielson de Sousa Carvalho ◽  
Camila Carvalho Menezes ◽  
Ana Paula Peron
Keyword(s):  
Allium Cepa ◽  
Cellular Level ◽  
Root Tip ◽  
Sunset Yellow ◽  
Cytotoxicity Studies ◽  
Allium Cepa L ◽  
Food Dyes ◽  
Chi Squared ◽  
Root Meristematic Cells ◽  
Tip Cells

The objective of this study was to evaluate the cytotoxic effect of the food dyes sunset yellow, bordeaux red, and tartrazine yellow on the cellular cycle of Allium cepa L. Each dye was evaluated at the doses of 0.4 and 4.0 mL, at the exposure times of 24 and 48 hours in root tip cells of Allium cepa L. Slides were prepared and cells were analyzed during the whole cell cycle for cellular aberrations totaling 5,000 total cells for each dose evaluated. The mitotic index was calculated, and statistical analysis was performed using the Chi-squared test (p < 0.05). The results showed that the three dyes used under the evaluated doses and exposure times were cytotoxic to the cells of the system-test used. Further cytotoxicity studies should be conducted for additional results and a proper evaluation of the effect of these three dyes on a cellular level.

scholarly journals Genotoxicity and toxicity evaluations of ECF cellulose bleaching effluents using the Allium cepa L. Test

2012 ◽  
Vol 72 (3) ◽  
pp. 471-477 ◽  
Author(s):  
O. Roa ◽  
MC. Yeber ◽  
W. Venegas
Keyword(s):  
Toxic Effect ◽  
Allium Cepa ◽  
Chromosomal Aberration ◽  
Root Cells ◽  
Bleaching Process ◽  
Allium Cepa L ◽  
Wastewater Quality ◽  
Genotoxicity Tests

Toxicity and genotoxicity tests were performed on root cells of Allium cepa in order to evaluate wastewater quality following an ECF cellulose bleaching process. The results revealed a toxic effect of the effluent, with inhibition of meristem growth and generally lower values of metaphase, anaphase and telophase indices at pH 10.5 than pH 7 for all effluent concentrations. The genotoxicity effect was different from the toxic effect given that the micronucleus and the chromosomal aberration tests in anaphase-telophase cells were low over all ranges of the studied effluent concentrations.

scholarly journals Detoxification of sunset yellow and brilliant blue dyes using soybean peroxidases

2021 ◽  
pp. 100215
Author(s):  
Shweta Sharma ◽  
Nithya Rani R ◽  
Vadiraj K.T. ◽  
Ravi MB ◽  
Chandan S ◽  
...  
Keyword(s):  
Brilliant Blue ◽  
Sunset Yellow

scholarly journals Considerations on the Toxicity of Brilliant Blue FCF Aqueous Solutions before and after Ozonation

2020 ◽  
Vol 71 (4) ◽  
pp. 356-365
Author(s):  
Maria Marcvart Tiron ◽  
Irina Eugenia Lucaciu ◽  
Mihai Nita-Lazar ◽  
Stefania Gheorghe
Keyword(s):  
Toxic Effect ◽  
Aquatic Organisms ◽  
Brilliant Blue ◽  
Ozone Treatment ◽  
Brilliant Blue Fcf ◽  
Food Dyes ◽  
Before And After ◽  
Effect Level ◽  
Ozonation Process ◽  
By Products

The food dyes have a potential toxic effect on aquatic organisms which trigger the necessity of their removal from industrial or urban wastewaters. Many different removal methods were investigated for this purpose, but the ozonation and advanced oxidation processes (AOPs) were successfully applied in this field. However, the majority of studies emphasized that color removal by ozonation process did not report a complete mineralization of the dye and the resulted by-products may have a greater toxicity than the original compound. In this context, the paper presents a comparative ecotoxicity study of the dye Brilliant Blue FCF (BB FCF) before and after ozone treatment. The BB FCF toxic effect, before and after ozonation was investigated on crustacean (Daphnia magna), lethal or inhibitory concentrations for 50% of tested organisms (LC50 / EC50) were used to estimate the effect level. The dye showed no toxicity on crustacean (CL50/CE50]100mg/L) before ozonation. The ozonized solutions presented a high toxicity for crustaceans compared to initial dye due to the by-products occurrence.

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