Opuntia ficus-indica cladode extract is an embryotoxic, larvicidal, and oviposition-deterrent agent for the diamondback moth, Plutella xylostella

2021 ◽  
Vol 139 ◽  
pp. 105351
Author(s):  
Elaine Cristina Batista Ferrreira ◽  
Isabella Coimbra Vila Nova ◽  
Welton Aaron de Almeida ◽  
Francieli Marcelino dos Santos Albuquerque ◽  
Glaucilane dos Santos Cruz ◽  
...  
2021 ◽  
Vol 13 (7) ◽  
pp. 47
Author(s):  
Cristiane G. L. Ruiz ◽  
Marilza S. Costa ◽  
Santino Seabra Jr ◽  
Mônica J. B. Pereira

Collard greens are commonly grown in family farming systems; however, damage caused by the diamondback moth Plutella xylostella (Lepidoptera: Plutellidae) reduces yields, leading to successive applications of insecticides and consequently, environmental and toxicological problems. Therefore, it is essential to search for alternatives that reduce the use of pesticides and are economically viable and accessible to small farmers. This study was aimed at evaluating the insecticidal activity of aqueous extracts of neem and tobacco on P. xylostella. First instar caterpillars were offered collard leaf discs treated with different concentrations (30 caterpillars per treatment) and after the third day, mortality was evaluated. To evaluate ovicidal properties, collard leaves with 30 eggs were immersed in extracts and after 48 h, viability was measured. Oviposition deterrent activity was assessed with 23 couples of P. xylostella released in cages (repetitions) containing treated collard plants and after 48 h, the number of eggs per plant was recorded. Neem and tobacco extracts exhibited larvicidal, ovicidal, and oviposition deterrent properties against P. xylostella, indicating that the use of these extracts may be promising alternatives in family farming systems.


1998 ◽  
Vol 33 (2) ◽  
pp. 239-241 ◽  
Author(s):  
Wawan Hermawan ◽  
Ritsuko Tsukuda ◽  
Shuhei Nakajima ◽  
Kenji Fujisaki ◽  
Fusao Nakasuji

2017 ◽  
Vol 43 (2) ◽  
pp. 195
Author(s):  
Robson Thomaz Thuler ◽  
Fernando Henrique Iost Filho ◽  
Hamilton César De Oliveira Charlo ◽  
Sergio Antônio De Bortoli

Plant induced resistance is a tool for integrated pest management, aimed at increasing plant defense against stress, which is compatible with other techniques. Rhizobacteria act in the plant through metabolic changes and may have direct effects on plant-feeding insects. The objective of this study was to determine the effects of cabbage plants inoculated with rhizobacteria on the biology and behavior of diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). Cabbage seeds inoculated with 12 rhizobacteria strains were sowed in polystyrene trays and later transplanted into the greenhouse. The cabbage plants with sufficient size to support stress were then infested with diamondback moth caterpillars. Later, healthy leaves suffering injuries were collected and taken to the laboratory to feed P. xylostella second instar caterpillars that were evaluated for larval and pupal viability and duration, pupal weight, and sex ratio. The reduction of leaf area was then calculated as a measure of the amount of larval feeding. Non-preference for feeding and oviposition assays were also performed, by comparing the control treatment and plants inoculated with different rhizobacterial strains. Plants inoculated with the strains EN4 of Kluyvera ascorbata and HPF14 of Bacillus thuringiensis negatively affected the biological characteristics of P. xylostella when such traits were evaluated together, without directly affecting the insect behavior.


2021 ◽  
Author(s):  
Yaohui Wang ◽  
Xia Xu ◽  
Xi’en Chen ◽  
Xiaowei Li ◽  
Honglun Bi ◽  
...  

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Shehzad ◽  
Muhammad Tariq ◽  
Tariq Mukhtar ◽  
Asim Gulzar

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.


Insects ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 109
Author(s):  
Norazila Yusoff ◽  
Idris Abd Ghani ◽  
Nurul Wahida Othman ◽  
Wan Mohd Aizat ◽  
Maizom Hassan

The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is the most important pest of cruciferous vegetables worldwide. In this study, we evaluated the properties of selected farnesyl derivative compounds against P. xylostella. The toxicity and sublethal concentration (LC50) of farnesyl acetate, farnesyl acetone, farnesyl bromide, farnesyl chloride, and hexahydrofarnesyl acetone were investigated for 96 h. The leaf-dip bioassays showed that farnesyl acetate had a high level of toxicity against P. xylostella compared to other tested farnesyl derivatives. The LC50 value was 56.41 mg/L on the second-instar larvae of P. xylostella. Then, the sublethal effects of farnesyl acetate on biological parameters of P. xylostella were assessed. Compared to the control group, the sublethal concentration of farnesyl acetate decreased pupation and emergence rates, pupal weight, fecundity, egg hatching rate, female ratio, and oviposition period. Furthermore, the developmental time of P. xylostella was extended after being exposed to farnesyl acetate. Moreover, the application of farnesyl acetate on P. xylostella induced morphogenetic abnormalities in larval–pupal intermediates, adults that emerged with twisted wings, or complete adults that could not emerge from the cocoon. These results suggested that farnesyl acetate was highly effective against P. xylostella. The sublethal concentration of farnesyl acetate could reduce the population of P. xylostella by increasing abnormal pupal and adults, and by delaying its development period.


2016 ◽  
Vol 132 ◽  
pp. 38-46 ◽  
Author(s):  
Shuzhen Zhang ◽  
Xiaolei Zhang ◽  
Jun Shen ◽  
Kaikai Mao ◽  
Hong You ◽  
...  

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