Detection of enteroparasites in foliar vegetables commercialized in street- and supermarkets in Aparecida de Goiânia, Goiás, Brazil

Foliar vegetables contaminated with fecal residues are an important route of transmission of intestinal parasites to humans. The aim of this study was to evaluate the presence of parasitic forms of protozoa and helminths on lettuces (Lactuca sativa) and collard greens (Brassica oleracea) sold in street- and supermarkets in the city of Aparecida de Goiânia, Goiás, Brazil. A total of 30 samples of each vegetable (15 samples from each supermarkets and street markets) was analyzed. All samples were processed by spontaneous sedimentation method and centrifugal flotation. In 45% of the samples, immature forms of intestinal parasites were identified with 66.7% helminths eggs and 33.3% protozoan cysts or oocysts. Significantly more lettuce samples were contaminated with eggs, cysts or oocyst of at least one parasite than collard green samples (U=216; Z=-3.45; P <0.001). The parasitic forms were identified morphologically up to the family level with eggs of Ancylostomatidae, Strongyloididae, Ascarididae and Taeniidae, or oocysts of Eimeriidae, to the genus with Cystoisospora sp. and Toxocara sp., and to the species level with Cystoisospora canis, Dipylidium caninum and Hymenolepis nana. The presence of these infective agents in lettuce and collard green from both street- and supermarkets highlights the high risk of spreading parasites by eating raw vegetables sold in Aparecida de Goiânia.

Plant-based expression of SARS-CoV-2 antigens for use in an oral vaccine

Oral and intra-nasal vaccines represent a key means of inducing mucosal-based immunity against infection with SARS-CoV-2, yet such vaccines represent only a minority of candidates currently in development. In this brief communication, we assessed the expression of the SARS-CoV-2 Receptor Binding Domain (RBD) subunit of the surface-exposed Spike (S) glycoprotein in the leaves of nine edible plant species (lettuce, spinach, collard greens, tomato, cucumber, radish, arugula, pepper, and Coho greens), with a goal of identifying a suitable candidate for the development of a oral vaccine against COVID-19. We report lettuce (Lactuca sativa L. cv. Hilde II Improved) to be a preferred host to support in planta expression of SARS-CoV-2 RBD, representing an important first step towards development of a plant-based oral vaccine.

CHICORY AND ARUGULA IN INTERCROPPING WITH COLLARD GREENS1

ABSTRACT Vegetable intercropping systems use complementarity between species to increase agricultural profitability. This study evaluated the effects of intercropping chicory and arugula species with collard greens on crop yield and land use efficiency (LUE). Six treatments, consisting of species planted as monocultures or intercropped in various combinations, were evaluated in a randomized block design with four replicates. The cultivars ‘Top Bunch’ (collard greens), ‘Pão de Açúcar’ (chicory) and ‘Folha Larga’ (arugula) were used. The yield of collard greens in monoculture did not differ from those obtained when they were intercropped with chicory, arugula, or both species, whereas chicory and arugula yields were higher in monoculture. However, even with yield losses for chicory and arugula in intercropping, LUE indices were greater than 1.0 in all intercropping systems, indicating their viability. The highest LUE index (2.41) was obtained in the chicory-arugula-collard green intercropping system.

Collard greens and chicory intercropping efficiency as a function of chicory (Cichorium intybus) transplant time

Vegetable intercropping has advantages over single cultivation in terms of less environmental impact. However, to convince farmers to adopt this production system, it is necessary to prove greater efficiency in the production of more food per unit area and therefore an increase in productivity. An experiment was carried out aiming to evaluate the effect of the chicory transplant time in intercrops with collard greens on crop yields and land use efficiency index (LUE). The experimental design was a randomized block, with nine treatments in a 2 × 4 + 1 factorial scheme, and four replications. Crop systems (intercrop and monoculture) and chicory transplant time (0, 14, 28 and 42 days after transplant (DAT) of collard greens) were evaluated. The collard greens yield increased as the chicory transplant time was delayed. The total and per harvest yields of chicory were not influenced by its transplant time. Regardless of chicory transplant time, collard greens and chicory intercropping provided greater LUE than their monocultures and reached the maximum value (52% higher) when the chicory was transplanted 42 days after collard greens. Highlights: The collard green yield increased by 11 kg ha-1 for each day of delay in the chicory transplant (0 to 42 days). The total and per harvest chicory yields were not influenced by its transplant time in relation to collard green transplant. The collard green and chicory intercropping provided 52% higher land use efficiency than their monocultures when the chicory was transplanted 42 days after collard green.

Evaluation of Chemical Seed Treatment to Reduce Injury Caused by Preemergent Herbicides on Direct-seeded Turnips and Collard Greens

Poor competitive ability and limited herbicide options make weed management of Brassica crops difficult. Growers often adopt the use of transplants, which is less efficient in terms of time, material, and labor when compared with direct seeding, resulting in higher prices per unit. Seed treatment with protective compounds could decrease crop injury from preemergent (PRE) herbicides making it profitable to direct-seed Brassica plants for production. Research was conducted to evaluate the ability of three candidate safeners [24-epibrassinolide, melatonin, and ascorbic acid (AsA)] to reduce injury caused by four herbicides (S-metolachlor, pyroxasulfone, halosulfuron, and mesotrione) applied PRE on the collard green cultivar Top Bunch and turnip cultivar Purple Top White Globe. Two independent greenhouse trials were conducted at the Clemson University Coastal Research and Education Center in Charleston, SC. Visual injury of the treated plants was evaluated weekly and dry mass was collected 21 days after treatment. Seed treatment did not reduce injury efficiently caused by pyroxasulfone, halosulfuron, and mesotrione; all doses were lethal for both crops. However, collard seeds treated using melatonin and AsA had 66% and 54% less injury caused by S-metolachlor at 514 g⋅ha–1 a.i., respectively. On turnips, melatonin was the only treatment that reduced the S-metolachlor damage on seedlings, with 43% less injury than untreated seedlings. Plant injury and plant weight correlated significantly for both Brassica crops. The reduction in injury caused by S-metolachlor when seeds were treated with melatonin and AsA validated those compounds’ protective ability. Seed treatment with melatonin could be combined with PRE applications of S-metolachlor to overcome the low weed competitive ability of these species early in the season.

Patterns of Nutrient Dynamics within and below the Rootzone of Collard Greens Grown under Different Organic Amendment Types and Rates

The knowledge about nutrient dynamics in the soil is pivotal for sustainable agriculture. A comprehensive research trial can retort unanswered questions. Dynamics of nutrients sourced from organic amendment types (chicken manure, dairy manure, and MilorganiteTM) applied at different rates (0, 168, 336, 672 kg total N/ha) were monitored within and below the rootzone of collard greens cultivated on a sandy loam soil in Prairie View, TX, USA. Macro- and micronutrients (e.g., TN: total nitrogen, P: phosphorous, K: potassium, Na: sodium, Ca: calcium, Mg: magnesium, B: boron, Cu: copper, Fe: iron, and Zn: zinc) were analyzed from soil solution samples collected during six sampling periods from within and below the rootzone. As hypothesized, the organic amendment types and rates significantly (p < 0.05 and/or 0.01) affected nutrient dynamics within and below the crop rootzone. Chicken manure released significantly more TN, P, K, Na, Ca, Mg, B, Cu, and Fe than the other two amendments. The application of chicken manure and MilorganiteTM resulted in higher below-the-rootzone leachate concentration of TN, Na, Mg, and Ca than in the leachates of dairy manure. Dairy manure treatments had the lowest concentrations of TN, Ca, and Mg; whereas, MilorganiteTM had the lowest concentrations of P, K, Na, B, and Cu in the collected leachates. The higher level of P (i.e., 4% in MilorganiteTM as compared to 2 and 0.5% in chicken and dairy manures, respectively, might have reduced the formation of Vesicular-Arbuscular (VA) mycorrhizae—a fungus with the ability to dissolve the soil P, resulting in slow release of P from MilorganiteTM treatment than from the other two treatments. Patterns of nutrient dynamics varied with rain and irrigation events under the effects of the soil water and time lapse of the amendment applications’ rates and types. All the macronutrients were present within the rootzone and leached below the rootzone, except Na. The dynamic of nutrients was element-specific and was influenced by the amendments’ type and application rate.

Production of Natural Insecticide on Management of Plutella xylostella (Linnaeus, 1758) (Lepidoptera: Plutellidae) in Family Farming

Collard greens are commonly grown in family farming systems; however, damage caused by the diamondback moth Plutella xylostella (Lepidoptera: Plutellidae) reduces yields, leading to successive applications of insecticides and consequently, environmental and toxicological problems. Therefore, it is essential to search for alternatives that reduce the use of pesticides and are economically viable and accessible to small farmers. This study was aimed at evaluating the insecticidal activity of aqueous extracts of neem and tobacco on P. xylostella. First instar caterpillars were offered collard leaf discs treated with different concentrations (30 caterpillars per treatment) and after the third day, mortality was evaluated. To evaluate ovicidal properties, collard leaves with 30 eggs were immersed in extracts and after 48 h, viability was measured. Oviposition deterrent activity was assessed with 23 couples of P. xylostella released in cages (repetitions) containing treated collard plants and after 48 h, the number of eggs per plant was recorded. Neem and tobacco extracts exhibited larvicidal, ovicidal, and oviposition deterrent properties against P. xylostella, indicating that the use of these extracts may be promising alternatives in family farming systems.