Sperm cryopreservation of the critically endangered olive barb (Sarpunti) Puntius sarana (Hamilton, 1822)

Cryobiology ◽  
2011 ◽  
Vol 62 (1) ◽  
pp. 62-67 ◽  
Author(s):  
M. Nahiduzzaman ◽  
M. Mahbubul Hassan ◽  
U. Habiba Khanam ◽  
S.N.A. Mamun ◽  
Mostafa A.R. Hossain ◽  
...  
Keyword(s):  
Critically Endangered ◽  
Sperm Cryopreservation ◽  
Puntius Sarana

Development of a Sperm Cryopreservation Protocol for Critically Endangered Mohashol, Tor tor (Hamilton)

2022 ◽  
Author(s):  
M. Salah Uddin Kabir ◽  
M. Rafiqul Islam Sarder ◽  
M. Matiur Rahman ◽  
M. Fazlul Awal Mollah ◽  
N. Binte Ryhan
Keyword(s):  
Critically Endangered ◽  
Sperm Cryopreservation ◽  
Cryopreservation Protocol ◽  
Tor Tor

scholarly journals Assessment of semen quality, sperm cryopreservation and heterologous IVF in the critically endangered Iberian lynx (Lynx pardinus)

2009 ◽  
Vol 21 (7) ◽  
pp. 848 ◽  
Author(s):  
Natalia Gañán ◽  
Raquel González ◽  
J. Julián Garde ◽  
Fernando Martínez ◽  
Astrid Vargas ◽  
...  
Keyword(s):  
Semen Quality ◽  
Statistical Significance ◽  
Egg Yolk ◽  
Domestic Cat ◽  
Critically Endangered ◽  
Lynx Lynx ◽  
Sperm Cryopreservation ◽  
Iberian Lynx ◽  
Lynx Pardinus

Semen traits and factors affecting sperm cryopreservation were assessed in the Iberian lynx (Lynx pardinus), a species regarded as the most endangered felid in the world. For cryopreservation, semen was washed, resuspended in a Tes–Tris-based diluent (TEST) or a Tris-based diluent (Biladyl), both with 20% egg yolk and 4% glycerol, loaded into straws, cooled to 5°C using an automated programmable system and frozen on nitrogen vapour. Heterologous IVF of in vitro-matured domestic cat oocytes was used to test the fertilising ability of cryopreserved spermatozoa. Electroejaculates from five males were obtained. Characterisation of the electroejaculates revealed mean (± s.e.m.) values of 3.3 ± 0.6 × 106 total spermatozoa, 73.6 ± 4.6% motile spermatozoa, 23.7 ± 4.0% morphologically normal spermatozoa and 40.7 ± 2.3% spermatozoa with intact acrosomes. After thawing a higher percentage of motile spermatozoa was seen in TEST than in Biladyl (34.0 ± 6.2% v. 7.5 ± 4.8%, respectively; P < 0.05); however, there were no differences in the percentage of intact acrosomes between the two diluents. Iberian lynx spermatozoa fertilised domestic cat oocytes in vitro, with higher fertilisation rates observed for spermatozoa cryopreserved in TEST than in Biladyl, although the difference did not reach statistical significance (20.5 ± 4.5% v. 11.5 ± 6.8%, respectively). There were positive significant relations between the fertilisation rates and both the percentage of normal spermatozoa and the percentage of spermatozoa with an intact acrosome before cryopreservation (P = 0.04). This first report of the collection and cryopreservation of Iberian lynx semen and analysis of fertilising ability is an important step in the development of assisted reproductive techniques for this critically endangered felid species.

scholarly journals Conservation of Critically Endangered Olive Barb Puntius sarana (Hamilton, 1822) through Artificial Propagation

Our Nature ◽  
2014 ◽  
Vol 11 (2) ◽  
pp. 96-104 ◽  
Author(s):  
Muhammad Abu Bakar Siddik ◽  
Ashfaqun Nahar ◽  
Ferdous Ahamed ◽  
Zubia Masood ◽  
Md. Yeamin Hossain
Keyword(s):  
Body Weight ◽  
Critically Endangered ◽  
Hatching Rate ◽  
Breeding Performance ◽  
Endangered Fish ◽  
Artificial Propagation ◽  
Special Diet ◽  
The Family ◽  
Puntius Sarana ◽  
Experimental Ponds

The Olive barb Puntius sarana (Hamilton, 1822) is a member of the family Cyprinidae and its conservational status has been referred as critically endangered in Bangladesh and vulnerable in India. An experiment on artificial propagation of the critically endangered fish P. sarana was carried out to determine the suitable dose of pituitary gland (PG) hormone as well as to determine the effective breeding season for the conservation of this critically endangered fish species. Three breeding trials (April 2010, June 2010 and July 2010) have been taken into consideration with PG doses in three different treatments (4.5, 5.5, 6.5 mg/kg body weight, respectively) having three replications of each. Brood fishes were collected from the Kangsha River (Netrokona) and reared in the experimental ponds, providing special diet upto their maturation. A total of 18 broods (9 female and 9 male) were selected for induced breeding in each trial. To observe the effective dose for induced breeding, the females were first injected at the rate of 4.5, 5.5 and 6.5 mg PG/kg body weight in T1, T2 and T3, respectively in each trial. On the other hand, the males were administrated at the rate of 2 mg PG/kg body weight. The breeding performance in terms of ovulation, fertilization and hatching rate were studied. Induced breeding, in trial-2 obtained the better result in terms of ovulation, fertilization and hatching rate compared to other two trials. In trial-2 among the treatments, T2 with doses of 5.5 mg/kg body weight showed better result than other two treatments where 4.5 and 6.5 mg/kg body weight PG doses were used in T1 and T3, respectively. The findings of the present study can be used in induced breeding of P. sarana for the development of hatchery propagation as well as to conserve this valuable critically endangered species.DOI: http://dx.doi.org/10.3126/on.v11i2.9534 Our Nature 2013, 11(2): 96-104

scholarly journals Improved Sperm Cryopreservation in the Critically Endangered Przewalski's Horse (Equus ferus przewalskii) Using Different Cryoprotectants.

2010 ◽  
Vol 83 (Suppl_1) ◽  
pp. 675-675 ◽  
Author(s):  
Budhan S. Pukazhenthi ◽  
Francesca Lapiana ◽  
Luis Padilla ◽  
Jenny Santiestevan ◽  
Marco Coutinho da Silva ◽  
...  
Keyword(s):  
Critically Endangered ◽  
Sperm Cryopreservation ◽  
Przewalski's Horse ◽  
Przewalski’S Horse

Genetic Diversity and Structure of the Nile Soft-Shelled Turtle (Trionyx triunguis) Along the Mediterranean coast of Turkey

2020 ◽  
Vol 27 (6) ◽  
pp. 307-315
Author(s):  
Özgür Güçlü ◽  
Bülent Bozdoğan
Keyword(s):  
Microsatellite Loci ◽  
Geographic Distance ◽  
Iucn Red List ◽  
Genetic Variations ◽  
Mediterranean Coast ◽  
Critically Endangered ◽  
Mediterranean Populations ◽  
Genetic Diversity And Structure ◽  
Polymorphic Microsatellite ◽  
The Mediterranean

The Nile soft-shelled turtle (Trionyx triunguis) is distributed between Dalyan and Samandağ throughout the Mediterranean coast in Turkey. The Mediterranean subpopulation of the Nile soft-shelled turtle is listed as critically endangered in the IUCN Red List Categories. This investigation aimed to determinate levels of genetic variations and patterns of genetic structures among Mediterranean populations in Turkey by using T. triunguis-specific microsatellite primers. A total of 13 polymorphic microsatellite loci were studied among samples of 121 individuals collected from five populations in Turkey. Of 13 polymorphic microsatellite loci used, 3 new were identified in this study. The genetic differentiation among the 5 studied populations of T. triunguis was significant (p 0.001). The analysis of molecular variance (AMOVA) indicated that genetic variations occurred mainly within populations (89.7%) rather than among populations (10.3%). Structure analysis showed presence of two main groups among the Mediterranean T. triunguis populations. However genetic variations among populations were not correlated with geographic distance between the locations. Analysis of data showed that one of the populations (Dalyan) had undergone a bottleneck effect. Migration analysis indicates that T. triunguis migrates between five Mediterranean populations in Turkey. We concluded that based on our results the status of ‘critically endangered’ of T. triunguis should be maintained. Long term population genetic survey studies should be undertaken and changes in habitats of T. triunguis populations, as well as their population size and structure should be monitored for each population to be able to establish a clear strategy for protection of T. triunguis.

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