scholarly journals Conservation of Critically Endangered Olive Barb Puntius sarana (Hamilton, 1822) through Artificial Propagation

Our Nature ◽  
2014 ◽  
Vol 11 (2) ◽  
pp. 96-104 ◽  
Author(s):  
Muhammad Abu Bakar Siddik ◽  
Ashfaqun Nahar ◽  
Ferdous Ahamed ◽  
Zubia Masood ◽  
Md. Yeamin Hossain

The Olive barb Puntius sarana (Hamilton, 1822) is a member of the family Cyprinidae and its conservational status has been referred as critically endangered in Bangladesh and vulnerable in India. An experiment on artificial propagation of the critically endangered fish P. sarana was carried out to determine the suitable dose of pituitary gland (PG) hormone as well as to determine the effective breeding season for the conservation of this critically endangered fish species. Three breeding trials (April 2010, June 2010 and July 2010) have been taken into consideration with PG doses in three different treatments (4.5, 5.5, 6.5 mg/kg body weight, respectively) having three replications of each. Brood fishes were collected from the Kangsha River (Netrokona) and reared in the experimental ponds, providing special diet upto their maturation. A total of 18 broods (9 female and 9 male) were selected for induced breeding in each trial. To observe the effective dose for induced breeding, the females were first injected at the rate of 4.5, 5.5 and 6.5 mg PG/kg body weight in T1, T2 and T3, respectively in each trial. On the other hand, the males were administrated at the rate of 2 mg PG/kg body weight. The breeding performance in terms of ovulation, fertilization and hatching rate were studied. Induced breeding, in trial-2 obtained the better result in terms of ovulation, fertilization and hatching rate compared to other two trials. In trial-2 among the treatments, T2 with doses of 5.5 mg/kg body weight showed better result than other two treatments where 4.5 and 6.5 mg/kg body weight PG doses were used in T1 and T3, respectively. The findings of the present study can be used in induced breeding of P. sarana for the development of hatchery propagation as well as to conserve this valuable critically endangered species.DOI: http://dx.doi.org/10.3126/on.v11i2.9534 Our Nature 2013, 11(2): 96-104

2012 ◽  
Vol 9 (2) ◽  
pp. 305-310
Author(s):  
MM Rahman ◽  
MI Miah ◽  
KR Hasan ◽  
SM Farid

Artificial propagation of Mastacembelus pancalus was conducted in order to determine the optimum dose of pituitary  gland (PG) hormone at the hatchery of the Field Laboratory Complex, Faculty of Fisheries, Bangladesh Agricultural  University, Mymensingh. The present study consisted of two trials and each trial had four treatments (T1, T2, T3 and  T4, respectively) with three replications of each. In the experiment single dose and double dose had been used for T1,  T2, T3 and T4, respectively to evaluate the efficiency on ovulation rate, fertilization rate, and hatching rate of M.  pancalus eggs. The hatchlings were reared in aquarium up to 30 days and survival rate was determined. Treatment-3  (T3) of double dose (170mg PG kg-1 and 60mg PG kg-1 body weight of fish for female and male respectively) showed  better results in terms of ovulation rate (90.03±2.56%), fertilization rate (90±0.81%), hatching rate (80±1.84%), and  survival rate (40.00±3.19%) was recorded than other treatments of single and double dose. The water temperature  was recorded 27 to 31°C. In the present study, treatment-3 (T3 in double dose) was the best findings in terms of  ovulation rate, fertilization rate, and hatching rate which can be used in artificial propagation of M. pancalus for the  development of hatchery production. May and June are the suitable months for artificial propagation of the said  species.   DOI: http://dx.doi.org/10.3329/jbau.v9i2.11045   J. Bangladesh Agril. Univ. 9(2): 305–310, 2011


Author(s):  
S Hayat ◽  
M Ramzan ◽  
M Zafarullaf ◽  
I Ahmad ◽  
Q Ali ◽  
...  

In present study the Effects of different doses of ovaprim on induced spawning activities of Rita rita were observed with an aim to standardize the dose of ovaprim for successful breeding. In the trial experiment, the females of Rita rita were treated with ovaprim at the rate of 0.5, 0.8 and 1.0ml/kg body weight and males of Rita rita in all the cases were treated at the rate of 0.4ml/kg.  The doses of ovaprim at the rates of 0.5, 0.8, 1.0 ml/kg body weight resulted in 0%, 100% and 100% ovulation respectively. In case of injection of ovaprim at the rate of 0.8ml/kg, the fertilization rate was observed to be about 70% and hatching rate was 58%, which occurred within 20-25 hours after fertilization at water temperature of 270C-290C. In case of injection of ovaprim at the rate of 1.0ml/kg, 18% fertilization rate and 5% hatching rate was observed. The doses of ovaprim at the rates of 0.8ml/kg and 0.4ml/kg body weight for female and male Rita rita respectively was found to be satisfactory for overall breeding performance and the commercially successful production of this fish. Hatchlings from over all experiment were reared up to fingerlings size to about 2 month and were finally stocked into the Chashma lake.


2013 ◽  
Vol 31 ◽  
pp. 23-26
Author(s):  
Jesmin Ahktar ◽  
Abdus Salam Bhuiyan

An experiment on the induced breeding of the endangered fish, Labeo calbasu (Hamilton-Buchanam) was conducted in the Fish Seed Multiplication Farm,Rajshahi to know the efficacy of two inducing agents (PG and DoM+SGnRH). Three breeding trials of each inducing agent were performed. A total of 24 females weighing from 1.5 kg to 2 kg were given an initial and a resolving dose of 1.5 mg and 6 mg PG extract per kg body weight respectively as treatment-1. On the other hand, a total of 24 females weighing from 1.5 kg to 2 kg were given a single dose of 12 mg DoM + SGnRH/kg as treatment-2. In case of treatment-1, 12 males weighing from 1.5 kg to 1.95 kg were administered a single dose of 1.5 mg PG/kg body weight during resolving dose of female. In treatment-2, 12 males weighing from 1.5 kg to 1.8 kg were administered 3 mg DoM+SGnRH /kg body weight during initial dose of females. In treatment-1, the time interval between initial and resolving dose was 5 hours and ovulation occurred in all the injected females within 6 hours after resolving dose. Ovulation occurred within 6 to 8 hours after the injection of inducing agents for treatment-2. The mean rates of ovulation, fertilization and hatching were 100%, 77.36% and 74.5% respectively in treatment-1. On the contrary, the mean rates of ovulation, fertilization and hatching were 83.33%, 63.83% and 59.66% in treatment-2. Hatchery produced fry were reared in nursery pond for 40 days. In nursery pond. Flour, oil cake and wheat bran were applied as nursery feeds. Both the inducing agents were effective in respect of overall breeding performance. But the best results were obtained with PG although in case of DoM+SGnRH complete breeding takes place within short time with less labour and cost than that of PG.DOI: http://dx.doi.org/10.3329/ujzru.v31i0.15376Univ. j. zool. Rajshahi Univ. Vol. 31, 2012 pp. 23-26


1970 ◽  
Vol 7 (1) ◽  
pp. 183-191 ◽  
Author(s):  
A Roy ◽  
MFA Mollah

To observe the effects of vitamin E on the ovarian development and breeding performances of Clarias batrachus an experiment was conducted in two phases. The first phase concentrated on studying the effects of vitamin E on ovarian development and the second phase on breeding performances. Eighty female C. batrachus broodfish were stocked in 8 cisterns (2.38 × 1.45m2 each) divided into 4 treatments each having two replicates. Each of the cistern was stocked with 10 females. The fish were fed with feed having different levels of vitamin E viz. 0 mg (served as control), 50 mg, 100 mg and 200 mg vitamin E/kg feed for 3 months to study the effects of vitamin E on the growth and ovarian development of C. batrachus broodfish. It was found that growth in terms of body weight of fish fed with 200 mg vitamin E/kg feed (under T4) was higher, while 50 mg vitamin E/kg feed (under T2) gave the poorest result. There was no significant difference among different treatments. Gonadosomatic index and fecundity were highest in fish treated with 100 mg vitamin E/kg feed (under T3), but there was no significant difference among the treatments. After rearing for 3 months fish were used for induction of breeding to study breeding performance. A pituitary gland (PG) dose of 100 mg/kg body weight was used in all treatments. Ovulation rate was 100% in females of all treatments. In case of fertilization and hatching rate the highest result (88.33±2.51 and 82.33±3.05 respectively) was obtained in T2 (50 mg vitamin E / kg feed), but there was no significant difference between T2 and T3. The overall result of this experiment indicates that 50 mg vitamin E/kg feed is the best dose for the breeding performance of female C. batrachus broods. Keywords: Clarias batrachus; Vitamin E; Ovarian development; Breeding performance; Larval growth DOI: 10.3329/jbau.v7i1.4983 J. Bangladesh Agril. Univ. 7(1): 183-191, 2009


2013 ◽  
Vol 19 (2) ◽  
pp. 111-118
Author(s):  
SM Farid ◽  
MI Miah ◽  
MAB Habib ◽  
MM Rahman

Five hormone doses viz., 40, 60, 70, 85 and 90 mg of Pituitary gland (PG)/kg body endangered Tarabaim (Macrognathus aculeatus) were tested and they were designated as Treatments T1, T2, T3, T4 and T5, respectively. Significantly (p<0.05) higher fertilization (86%) and hatching rates (50%) were obtained from T5 (90 mg of PG extract/kg body weight) than those of other treatments. However, T4 (85 mg of PG extract/kg, body weight) and T3 (70 mg of PG extract/kg body weight) gave the highest (90%) ovulation rate. In May and June, the highest fertilization rate of 82% and 86% respectively and hatching rate 45% and 75%, respectively were obtained at the temperature ranged from 27.0 to 33.0ºC. In conclusion, PG extract at a dose of 90 mg/kg body weight appeared to be the suitable dose for artificial propagation of M. aculeatus, May and June are the suitable months for its artificial propagation.DOI: http://dx.doi.org/10.3329/pa.v19i2.16951 Progress. Agric. 19(2): 111 - 118, 2008


2020 ◽  
Vol 7 (2) ◽  
pp. 178-181
Author(s):  
Lalnun Mawia ◽  
Vanlalhruaii Ralte ◽  
H. Lalruatsanga ◽  
Zothan Mawia ◽  
P.C. Vanlalhluna ◽  
...  

Globba wengeri (C.E.C. Fisch.) K.J. Williams, former state flower of Mizoram, a rare and critically endangered plant species, commonly known as ‘dancing girl’, belonging to the family Zingiberaceae, is reported in this communication for the first time from Serchhip District in Mizoram at an elevation of about 1187 m a.s.l. It was found on moist, watery and rocky slopes. The plant is under severe threat in the natural habitat and therefore, further studies are required to determine life history and particular survival threats of this species.


2018 ◽  
Vol 13 (3) ◽  
pp. 213
Author(s):  
Jojo Subagja ◽  
Vitas Atmadi Prakoso

Ikan baung (Hemibagrus nemurus) memiliki prospek bagus untuk dikembangkan sebagai komoditas budidaya di Indonesia karena citarasa daging yang enak banyak diminati konsumen. Dewasa ini, peningkatan produksi benih dapat dilakukan melalui pemijahan buatan dengan manipulasi hormonal. Penelitian yang dilakukan bertujuan untuk mengevaluasi produksi induk ikan baung melalui pemijahan buatan dengan dosis penyuntikan GnRH-a berbeda. Penelitian dilakukan di Instalasi Riset Plasma Nutfah Perikanan Air Tawar, Cijeruk, Bogor pada bulan November 2017. Induk betina ikan baung yang digunakan berukuran 465,0 ± 71,8 g; dan induk jantan 426,3 ± 46,8 g. Induksi dilakukan dengan penyuntikan hormon GnRH-a dengan tiga dosis yang berbeda pada induk betina (0,3; 0,5; dan 0,7 mL/kg bobot badan), dan ikan jantan dengan dosis 0,4 mL/kg bobot badan. Masing-masing perlakuan terdiri atas tiga ekor induk betina sebagai ulangan. Hormon disuntikkan secara intramuskular, diberikan dua kali penyuntikan 35% dari dosis total pada penyuntikan pertama, dan 65% diberikan pada penyuntikan kedua, dengan interval waktu penyuntikan delapan jam. Parameter yang diamati yaitu jumlah telur ovulasi, derajat pembuahan, derajat penetasan, dan sintasan larva selama tujuh hari. Hasil penelitian menunjukkan bahwa dosis penyuntikan hormon GnRH-a 0,5 mL/kg pada induk ikan baung saat proses pemijahan buatan menghasilkan derajat penetasan yang lebih tinggi dibandingkan dosis 0,3 dan 0,7 mL/kg; serta sintasan larva yang lebih baik dibandingkan dosis 0,7 mL/kg. Sedangkan nilai jumlah telur yang berhasil ovulasi dan derajat pembuahan yang relatif lebih baik ditemukan pada perlakuan dosis 0,7 mL/kg dibandingkan dengan dosis 0,3 dan 0,5 mL/kg.Asian redtail catfish (Hemibagrus nemurus) has a good prospect to be developed as aquaculture commodity in Indonesia. It was proved by the demand of consumers regarding to its delicious flesh taste. Recently, efforts to increase seedling production could be implemented through artificial spawning with hormonal manipulation. This study was conducted to evaluate the production of broodstocks through artificial spawning with different doses of GnRH-a. Study was conducted at Research Institute for Freshwater Fisheries Germplasm, Cijeruk, Bogor in November, 2017. The broodstock used in this study was 465.0 ± 71.8 g for females, and 426.3 ± 46.8 g for males. Induction was performed by injection of GnRH-a hormone with three different doses on female broodstocks (0.3, 0.5 and 0.7 mL/kg of body weight), and male broodstocks with a dose of 0.4 mL/kg of body weight. Each treatment consisted of three females as replications. The hormone was injected intramuscularly, given twice injection: 35% of the total dose is given at the first injection, and the remaining 65% was given at the second injection, with an injection time interval of eight hours. The results showed the observed parameters of number of the ovulated eggs, fertilization rate, hatching rate, and larval survival for seven days, treatment dosage of 0.5 mL/kg showed better results (P<0.05) than the dosage of 0.3 mL/kg and 0.7 mL/kg. Parameters observed were number of ovulated eggs, fertility rate, hatching rate, and survival rate for seven days. The results showed that the dosage of 0.5 mL/kg GnRH-a injection to the broodstock during artificial spawning resulted in higher hatching rate compared to 0.3 and 0.7 mL/kg and better larval survival rate than the dosage of 0.7 mL/kg. Meanwhile, the number of ovulated eggs and fertility rate was relatively better in the treatment of 0.7 mL/kg compared with the dosage of 0.3 and 0.5 mL/kg.


Author(s):  
S Sultana ◽  
MS Rahman ◽  
KA Jackshay ◽  
K Hasan

An experiment was carried out to evaluate the effects of different amounts of supplementary feeds on growth and production of fishes in polyculture where four different species such as rui (Labeo rohita), mrigal (Cirrhinus mrigala), common carp (Cyprinus carpio) and tilapia (Oreochromis niloticus) were cultured in 6 ponds under three treatments, each with two replications. Eighty fish per decimal were stocked along with the stocking ratio of 1:1:1:1 in all the treatments. Supplementary feeds of rice bran and wheat bran (1:1), at the amount of 5% of body weight were applied in 2 ponds (treatment I) as well as at the amount of 3% of body weight were used in 2 ponds (treatment II) and 2 ponds (treatment III) were as control where no supplementary feed was used. Urea and T.S.P were applied fortnightly at the rate of 60 g decimal-1 and 90 g decimal-1, respectively under all treatments. Physico-chemical and biological factors such as air and water temperature, transparency, rainfall, pH, dissolved oxygen, free CO2, total alkalinity, PO4-P and NO3-N, phytoplankton and zooplankton were studied fortnightly and the limnological conditions were more or less similar in the experimental ponds under three treatments. The calculated gross and net fish productions of fishes under treatment I, treatment II and treatment III were 8.87 and 7.33 ton ha-1 yr-1, 7.12 and 5.58 ton ha-1 yr-1 and 4.69 and 3.16 ton ha-1 yr-1, respectively. Percent increased of net fish productions of treatment I and treatment II were 231.97% and 176.59% higher than that of treatment III (control). The present study reveals that supplementary feeding in fish culture should be done along with fertilization because supplementary feeds are very important to enhance growth of fishes significantly. Int. J. Agril. Res. Innov. Tech. 10(2): 128-136, December 2020


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