Deep supercooling of water and preservation of cells at deep supercooled temperatures

Cryobiology ◽  
2020 ◽  
Vol 97 ◽  
pp. 255
Author(s):  
Osman Berk Usta ◽  
Haishui Huang ◽  
Camilo Rey-Bedon ◽  
Martin Yarmush
Keyword(s):  
Polymers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 347
Author(s):  
Wenlin Zhang ◽  
Lingyi Zou

We apply molecular dynamics (MD) simulations to investigate crystal nucleation in incompatible polymer blends under deep supercooling conditions. Simulations of isothermal nucleation are performed for phase-separated blends with different degrees of incompatibility. In weakly segregated blends, slow and incompatible chains in crystallizable polymer domains can significantly hinder the crystal nucleation and growth. When a crystallizable polymer is blended with a more mobile species in interfacial regions, enhanced molecular mobility leads to the fast growth of crystalline order. However, the incubation time remains the same as that in pure samples. By inducing anisotropic alignment near the interfaces of strongly segregated blends, phase separation also promotes crystalline order to grow near interfaces between different polymer domains.


1997 ◽  
Vol 77 (3) ◽  
pp. 399-420 ◽  
Author(s):  
Pauliina Palonen ◽  
Deborah Buszard

This article gives an overview of the current state of cold hardiness research in fruit crops by reviewing the recently published studies on cold hardiness of both tree fruit and berry crops. Topics discussed include cold hardiness of fruit species, cultivars and different plant organs, biophysical and biochemical aspects of hardiness, evaluation of hardiness, as well as endogenous, cultural and environmental factors affecting cold hardiness in these species. Lack of cold hardiness is a major limiting factor for production of fruit crops in many regions of the world and improved cold hardiness one of the major objectives in numerous breeding programs and research projects. Screening cultivars or selections for cold hardiness is commonly done, and different methods applied to the evaluation of hardiness are discussed. The physical limit of deep supercooling may be a restricting factor for expanding the production of some fruit crops, such as Prunus species and pear. As for biochemical aspects, a relationship between carbohydrates and cold hardiness is most commonly found. Studies have also been made on different hardiness modifying cultural factors including rootstock, crop load, raised beds and application of growth regulators. The latter seems promising for some species. Cold hardiness is an extremely complex phenomenon and understanding different mechanisms involved is critical. Since hardiness is, however, primarily affected by genotype, developing cold-hardy fruit cultivars and effective screening methods for hardiness are essential. Finally, cultural practices may be improved to further enhance hardiness. Key words: Berries, cold hardiness, fruits, small fruits, stress, winter hardiness


Planta ◽  
1991 ◽  
Vol 184 (2) ◽  
pp. 254-260 ◽  
Author(s):  
Michael Wisniewski ◽  
Glen Davis ◽  
Karen Schafter

2011 ◽  
Vol 1 (SRMS-7) ◽  
Author(s):  
J. K. R. Weber ◽  
C. J. Benmore ◽  
M. C. Wilding ◽  
J. Neuefeind ◽  
J. B. Parise

Containerless techniques (levitation) completely eliminate contact with the sample. This unique sample environment allows deep supercooling of many liquids and avoids contamination of high-temperature melts. Recent experiments at the Advanced Photon Source (APS) high-energy beamline 11 ID-C used aerodynamic levitation with laser beam heating and acoustic levitation with cryogenic cooling. By using these two methods, liquids were studied over much of the temperature range from −40 to +2500°C. This paper briefly describes the instrumentation and its use and is illustrated with examples of measurements on molten oxides and low-temperature liquids.


Planta ◽  
2011 ◽  
Vol 235 (4) ◽  
pp. 747-759 ◽  
Author(s):  
Donghui Wang ◽  
Jun Kasuga ◽  
Chikako Kuwabara ◽  
Keita Endoh ◽  
Yukiharu Fukushi ◽  
...  

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