Microbiological load and preantral follicle preservation using different systems for ovarian tissue vitrification in the red-rumped agouti

The objectives of this study were to investigate whether TGF-β affect the survival, activation and further growth of goat primordial follicles enclosed in ovarian cortex after in vitro culture. Goat ovaries were collected from an abattoir and pieces of ovarian tissues were cultured for one or seven days in a supplemented alpha Minimum Essential Medium, alone or containing TGF-β (1, 5, 10 or 50ng/mL). Ovarian tissues from the fresh control as well as those cultured were processed for histological and ultrastructural studies. The results showed that when compared with fresh control, there was decrease in the percentages of histologically normal follicles in all treatments only after seven days culture. TGF-β did not affect the activation of preantral follicles regardless of its concentration, however, larger follicles diameter (P<0.05) was observed using 10ng/mL TGF-β than in the fresh control and other treatments. Moreover, this concentration maintained the normal ultrastructure after seven days of culture. In conclusion, TGF-β showed additional effect on the follicle growth and the maintenance of ultrastructural integrity of goat preantral follicles enclosed in ovarian tissue when used at 10ng/mL during seven days of culture.

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Evaluation of protective effects of mirtazapine and mesna on cisplatin-induced ovarian damage in rats

Journal of Experimental and Clinical Medicine ◽

10.52142/omujecm.38.2.4 ◽

2021 ◽

Vol 38 (2) ◽

pp. 72-81

Author(s):

Önder SAKIN ◽

Ali Doğukan ANGIN ◽

Muhammet Ali ORUÇ ◽

Emine Eda AKALIN ◽

Muzaffer Seyhan CIKMAN ◽

...

Keyword(s):

Single Dose ◽

Ovarian Tissue ◽

Albino Rats ◽

Protective Effects ◽

Preantral Follicle ◽

Damage Score ◽

The Third ◽

Normal Ovarian Tissue ◽

Ovarian Damage ◽

Wistar Albino Rats

To evaluate whether mirtazapine and mesna have protective effects on cisplatin-induced ovarian injury. A total of 32 female Wistar Albino rats were divided into 4 groups (8 rats per group) and included in the study. No medication was administered to the first group; only intervention was that their ovaries were removed and anti-mullerian hormone (AMH) values were measured. The second group received intramuscular cisplatin at a single dose of 7.5 mg/kg. The third group received a single dose of 200 mg/kg mesna intraperitoneally, and 30 minutes later, a single dose of 7.5 mg/kg intramuscular cisplatin was administered. The fourth group received oral 30 mg/kg mirtazapine, and 60 minutes later, a single dose of 7.5 mg/kg intramuscular cisplatin was administered. Oral 30 mg/kg mirtazapine was continued for ten days. Ovaries and AMH values of all groups were evaluated at the end of tenth day. In the cisplatin group when compared to normal ovarian tissue total histopathological damage score increased (p=0.037), preantral follicle count decreased (p=0.003) and AMH levels decreased (p<0.001). In the cisplatin + mesna group total ovarian damage score was also increased (p=0.005), preantral and antral follicles decreased (p<0.001 and p=0.001, respectively), and AMH levels decreased (p<0.001). In the cisplatin + mirtazapine group, total ovarian damage score (p<0.001), preantral follicle count (p=0.002) and AMH values were decreased (p<0.001). It was concluded that mesna and mirtazapine were not effective in preventing ovarian damage due to cisplatin.

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44 Proliferation and Antral Formation of Preantral Follicle Within Cryopreserved Cat Ovarian Tissue Transplanted into Nude Mice

Reproduction Fertility and Development ◽

10.1071/rdv30n1ab44 ◽

2018 ◽

Vol 30 (1) ◽

pp. 161

Author(s):

N. Tanpradit ◽

K. Chatdarong

Keyword(s):

Nude Mouse ◽

Nude Mice ◽

Ovarian Tissue ◽

Domestic Cat ◽

Tissue Transplantation ◽

Proliferation Index ◽

Preantral Follicle ◽

Preantral Follicles ◽

Before And After ◽

Cryopreserved Ovarian Tissue

Transplantation of cryopreserved ovarian tissue is a fertility preservation technique that results in live births in patients who have undergone pelvic chemo- or radiotherapy. It can be used for conservation purposes in endangered animals by transplanting the cryopreserved ovarian tissue of animals with highly valuable genetics into the immunodeficient animal to grow the follicles. This study aimed to examine the preantral follicular viability and K i-67 proliferation index of the preantral follicles within fresh and cryopreserved cat ovarian tissue transplanted into nude mice as a model for endangered felids. Adult female nude mice (n = 9) were chosen to be the hosts of ovarian tissues from 2-year-old domestic cat. Ovarian cortical tissues were cut into 66 small pieces (1 × 1 × 0.2 mm3). Half of the fragments were cryopreserved using slow-freezing method with 1.5 M dimethyl sulfoxide (DMSO) and 0.1 M sucrose and thawed using thawing medium with 0.75 M DMSO and 0.5 M sucrose. Three pieces of fresh and cryopreserved tissues were transplanted into the subcutaneous pocket of the left and right side of the back of the nude mouse, respectively. Follicular viability and proliferation index were investigated after graft retrieval on Day 15 post-transplantation. Evaluation of preantral follicle viability was based on the integrity of the basement membrane, pyknotic bodies, and oocyte integrity. Proliferation index was determined by percentage of preantral follicle that had K i-67 immunopositive granulosa cells. Preantral follicle viability data was analysed using Kruskal-Wallis test and proliferation index data were analysed using general linear model test of least squares means. The percentages of viable follicles of all stages were decreased at Day 15 (Table 1). The percentage of proliferating preantral follicles in the fresh ovarian fragments was higher after transplantation (11 ± 2% and 46 ± 24% before and after transplantation, respectively; P < 0.05). However, the percentages of proliferating follicles were not different between before and after cryopreserved tissue transplantation (35 ± 8% and 45 ± 33%, respectively). In conclusion, our findings showed the possibility of domestic cat ovarian tissue transplantation into the nude mouse. although cryopreserved ovarian tissue did not tolerate the short-term transplantation as the fresh tissue. This approach needs further investigation to optimize the transplantation technique in terms of ischaemic reperfusion and neovascularization. Table 1.Percentage of viable follicles per area of 0.0625 mm2 within fresh and cryopreserved cat ovarian fragments at Day 0 and Day 15 of the study

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Preservation of bovine preantral follicle viability and ultra-structure after cooling and freezing of ovarian tissue

Animal Reproduction Science ◽

10.1016/j.anireprosci.2007.08.016 ◽

2008 ◽

Vol 108 (3-4) ◽

pp. 309-318 ◽

Cited By ~ 27

Author(s):

Juliana Jales de Hollanda Celestino ◽

Regiane Rodrigues dos Santos ◽

Cláudio Afonso Pinho Lopes ◽

Fabrício Sousa Martins ◽

Maria Helena Tavares Matos ◽

...

Keyword(s):

Ovarian Tissue ◽

Preantral Follicle ◽

Ultra Structure

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Healthy Early Preantral Follicle Can Be Obtained in a Culture of Frozen–Thawed Human Ovarian Tissue of 32 Weeks

Ultrastructural Pathology ◽

10.1080/01913120701515496 ◽

2007 ◽

Vol 31 (4) ◽

pp. 257-262 ◽

Cited By ~ 3

Author(s):

Raffaella Fabbri ◽

Gianandrea Pasquinelli ◽

Lorenzo Montanaro ◽

Bruno Mozzanega ◽

Valentina Magnani ◽

...

Keyword(s):

Ovarian Tissue ◽

Preantral Follicle ◽

Human Ovarian Tissue

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In vivo and in vitro strategies to support caprine preantral follicle development after ovarian tissue vitrification

Reproduction Fertility and Development ◽

10.1071/rd17315 ◽

2018 ◽

Vol 30 (8) ◽

pp. 1055 ◽

Cited By ~ 7

Author(s):

N. J. Donfack ◽

K. A. Alves ◽

B. G. Alves ◽

R. M. P. Rocha ◽

J. B. Bruno ◽

...

Keyword(s):

In Vitro Culture ◽

Ovarian Function ◽

Ovarian Tissue ◽

Preantral Follicle ◽

Hormone Production ◽

Treatment Groups ◽

Morphology Development ◽

Fresh Culture

The aim of the present study was to compare fresh and vitrified goat ovarian tissue after autotransplantation and in vitro culture. Adult goats were completely ovariectomised and each ovarian pair was sliced and distributed among six different treatment groups: fresh control, fresh transplant, fresh culture, vitrified control, vitrified transplant and vitrified culture. Follicular morphology, development, growth, density, revascularisation and hormone production were evaluated in all groups. Three antral follicles (two in the fresh transplant and one in the vitrified transplant groups) were observed on the surface of the graft 90 days after transplantation. The percentage of morphologically normal follicles was similar in the fresh control, fresh transplant and vitrified transplant groups. The percentage of developing (transition, primary and secondary) follicles was higher after in vitro culture of fresh or vitrified tissue. Transplantation resulted in a lower follicle density. Serum oestradiol concentrations remained constant during the entire transplantation period. In contrast, progesterone production decreased significantly. Expression of CD31 mRNA was lower in fresh culture. In conclusion, restoration of goat ovarian function can be successfully achieved following transplantation of both fresh and vitrified goat ovarian tissue. However, transplantation induced higher follicle loss than in vitro culture.

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In vitro follicular development of cryopreserved mouse ovarian tissue

Reproduction ◽

10.1530/rep.1.00515 ◽

2005 ◽

Vol 130 (2) ◽

pp. 187-192 ◽

Cited By ~ 27

Author(s):

Miwa Segino ◽

Mario Ikeda ◽

Fumiki Hirahara ◽

Kahei Sato

Keyword(s):

Cell Injury ◽

Ovarian Tissue ◽

Follicular Development ◽

Blastocyst Stage ◽

Preantral Follicle ◽

Hoechst 33258 ◽

Preantral Follicles ◽

Prolonged Culture ◽

Supravital Staining

In a previous report, we showed that follicles isolated from frozen/thawed mouse ovarian tissues reached the mature follicle stage on the 12th day of culture. However, the developmental ability was lower than that of fresh ovarian tissue. The purpose of this study was to define a culture system with some technical modification for preantral follicles isolated from frozen/thawed ovarian tissue and to confirm cell injury. Ovaries obtained from three-week-old female mice were cryopreserved by the rapid freezing method. Preantral follicles isolated from frozen/thawed ovarian tissues were cultured for 12–16 days. The follicles were then stimulated with human chorionic gonadotropin. In vitro fertilization was performed on the released cumulus–oocyte complexes (COCs). Preantral follicle viability was assessed by supravital staining using Hoechst 33258. Using this stain cell death was found in part of the granulosa cells of a follicle obtained from frozen/thawed ovarian tissue. On the 14th and 16th days of culture, the diameters of follicles isolated from frozen/thawed ovaries were larger than on the 12th day of culture. The released COCs were fertilized and developed to the blastocyst stage in 15.8% (12/76) of the oocytes taken from the fresh group, and in 0% (0/73), 2.9% (2/69) and 19.1% (22/115) of the oocytes taken from the frozen/thawed group that had been cultured for 12, 14 and 16 days respectively. The preantral follicles isolated from frozen/thawed mouse ovarian tissues developed slowly compared with the freshly prepared preantral follicles. During prolonged culture from 12 to 16 days, these follicles obtained the potential to fertilize and develop to the blastocyst stage.

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Cryopreservation of swine ovarian tissue: Effect of different cryoprotectants on the structural preservation of preantral follicle oocytes

Cryobiology ◽

10.1016/j.cryobiol.2009.07.003 ◽

2009 ◽

Vol 59 (2) ◽

pp. 195-200 ◽

Cited By ~ 43

Author(s):

E.N. Borges ◽

R.C. Silva ◽

D.O. Futino ◽

C.M.C. Rocha-Junior ◽

C.A. Amorim ◽

...

Keyword(s):

Ovarian Tissue ◽

Preantral Follicle ◽

Structural Preservation ◽

Tissue Effect

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Characterisation and cryopreservation of the ovarian preantral follicle population from Spix’s yellow-toothed cavies (Galea spixii Wagler, 1831)

Reproduction Fertility and Development ◽

10.1071/rd15249 ◽

2017 ◽

Vol 29 (3) ◽

pp. 594 ◽

Cited By ~ 6

Author(s):

Érica C. G. Praxedes ◽

Gabriela L. Lima ◽

Andréia M. Silva ◽

Carlos A. C. Apolinário ◽

José A. B. Bezerra ◽

...

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Light Microscopy ◽

Population Analysis ◽

Ovarian Tissue ◽

Morphological Aspect ◽

Preantral Follicle ◽

Transmission Electron ◽

The Mean ◽

The Right

The aim of the present study was to characterise the ovarian preantral follicle (PF) population and to establish a solid surface vitrification (SSV) process using dimethyl sulfoxide (DMSO) as a cryoprotectant for preservation of ovarian tissue from yellow-toothed cavies (Galea spixii). Ovaries were fixed for PF population analysis or were subjected to the SSV process. The mean (± s.e.m.) PF population per ovarian pair was estimated to be 416.0 ± 342.8. There were 140.0 ± 56.0 (63.4%) and 125.0 ± 58.0 (64.0%) primary follicles on the right and left ovaries, respectively. The proportion of this follicle category was significantly greater than that of other follicle categories (P < 0.05). The diameter of follicles (123.7 ± 18.3 µm), oocytes (50.1 ± 5.0 µm) and nuclei (14.27 ± 2.01 µm) was larger for secondary ones when compared with other PFs categories. Most PFs were morphologically normal (94.6%), with light microscopy identifying only a few atretic follicles (5.4%). After SSV, there was a reduction in the proportion of morphologically normal PFs compared with the non-vitrified group (69.5% vs 91.2%, respectively). Transmission electron microscopy revealed preservation of oocytes and granulosa cell membranes and the morphological aspect of follicles; the primary change observed in some vitrified PFs was the presence of vacuoles in the oocytes and granulosa cells cytoplasm and turgid mitochondria. In conclusion, the present study provides an estimative and characterization for the PF population in ovaries of G. spixii. Moreover, we report its PFs cryopreservation using an SSV process.

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