Evaluation of protective effects of mirtazapine and mesna on cisplatin-induced ovarian damage in rats

To evaluate whether mirtazapine and mesna have protective effects on cisplatin-induced ovarian injury. A total of 32 female Wistar Albino rats were divided into 4 groups (8 rats per group) and included in the study. No medication was administered to the first group; only intervention was that their ovaries were removed and anti-mullerian hormone (AMH) values were measured. The second group received intramuscular cisplatin at a single dose of 7.5 mg/kg. The third group received a single dose of 200 mg/kg mesna intraperitoneally, and 30 minutes later, a single dose of 7.5 mg/kg intramuscular cisplatin was administered. The fourth group received oral 30 mg/kg mirtazapine, and 60 minutes later, a single dose of 7.5 mg/kg intramuscular cisplatin was administered. Oral 30 mg/kg mirtazapine was continued for ten days. Ovaries and AMH values of all groups were evaluated at the end of tenth day. In the cisplatin group when compared to normal ovarian tissue total histopathological damage score increased (p=0.037), preantral follicle count decreased (p=0.003) and AMH levels decreased (p<0.001). In the cisplatin + mesna group total ovarian damage score was also increased (p=0.005), preantral and antral follicles decreased (p<0.001 and p=0.001, respectively), and AMH levels decreased (p<0.001). In the cisplatin + mirtazapine group, total ovarian damage score (p<0.001), preantral follicle count (p=0.002) and AMH values were decreased (p<0.001). It was concluded that mesna and mirtazapine were not effective in preventing ovarian damage due to cisplatin.

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An investigation of the protective effects of Dehydroepiandrosterone (DHEA) in chemotherapatic Cyclophosphamide (CP) induced ovarian damage on rats

Medical Science and Discovery ◽

10.36472/msd.v6i12.333 ◽

2019 ◽

Vol 6 (12) ◽

pp. 340-346

Author(s):

Önder Sakin ◽

Yasemin Alan ◽

Ali Doğukan Anğın ◽

Kahyan Basak ◽

Murat Alan

Keyword(s):

Single Dose ◽

Ovarian Tissue ◽

Test Group ◽

Antral Follicle ◽

Control Group ◽

Protective Effects ◽

Blood Samples ◽

Vascular Congestion ◽

Ovarian Damage ◽

Total Damage

Objective: We aimed to investigate whether cyclophosphamide-induced damage to rat ovary can be prevented by DHEA. Material and Method: Group 1 (the control Group): no treatment was administered. Intact ovarian tissue was removed and blood samples were taken for anti-mullerian hormone (AMH) test. Group 2 (the Cyclophosphamide Group): Rats received Cyclophosphamide intraperitoneally at a single dose of 150 mg / kg. Group 3 (the Cyclophosphamide + DHEA Group): Rats received Cyclophosphamide intraperitoneally at a single dose of 150 mg / kg at baseline and DHEA subcutaneously for 10 days at a dose of 60 mg / kg daily. Rats in groups 2 and 3 were sacrificed at the end of 10 days, ovarian tissues were removed and blood samples were taken for AMH test. Results: While normal ovarian tissue damage scores were zero, cyclophosphamide showed significant damage and histopathological changes in all rats. Cyclophosphamide group had higher vascular congestion (p=0.004) and total damage scores (p=0.010) than normal ovarian group. Cyclophosphamide + DHEA group had higher edema (p<0.001), vascular congestion (p<0.001) and total damage scores (p<0.001). Cyclophosphamide group had a decrease in primordial (p = 0.001), primary (p = 0.043) and preantral follicles(p = 0.006). Cyclophosphamide + DHEA group showed a decrease in primordial (p = 0.001) and antral follicles(p = 0.018). AMH levels did not decrease in both groups. Conclusions: It was found that the use of DHEA to prevent Cyclophosphamide-induced ovarian damage in rats did not produce significant changes in antral follicle counts, ovarian volume, and AMH levels, which were important for clinical practice.

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Protective Effect of Ethanolic Extract ofTabernaemontana divaricata(L.) R. Br. against DEN and Fe NTA Induced Liver Necrosis in Wistar Albino Rats

BioMed Research International ◽

10.1155/2014/240243 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 9

Author(s):

Kannappan Poornima ◽

Palanisamy Chella Perumal ◽

Velliyur Kanniappan Gopalakrishnan

Keyword(s):

Ethanolic Extract ◽

Treated Group ◽

Hepatic Necrosis ◽

Hepatoprotective Activity ◽

Albino Rats ◽

Protective Effects ◽

Liver Necrosis ◽

Standard Drug ◽

Tabernaemontana Divaricata ◽

Wistar Albino Rats

This study is an attempt to evaluate the hepatoprotective activity ofTabernaemontana divaricataagainst DEN and Fe NTA induced liver necrosis in rats. Ethanolic extract of the whole plant ofTabernaemontana divaricataat doses of 200 and 400 mg/kg body weight and 5-fluorouracil (standard drug) was orally administered to male Wistar Albino rats once daily for 24 weeks, simultaneously treated with the carcinogen DEN and Fe NTA. In simultaneously treated animals, the plant extract significantly decreased the levels of uric acid, bilirubin, AST, ALT, and ALP in serum and increased the levels of liver marker enzymes in liver. Treatment with the extracts resulted in a significant increase in the levels of antioxidants accompanied by a marked reduction in the levels of malondialdehyde when compared to DEN and Fe NTA treated group. When compared with 200 mg/kg bw rats, 400 mg/kg bw rats and 5-fluorouracil treated rats showed better results in all the parameters. The histopathological studies confirmed the protective effects of extract against DEN and Fe NTA induced liver necrosis. Thus, it could be concluded that the use ofTabernaemontana divaricataextract in the treatment of carcinogen induced hepatic necrosis.

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Protective effects of rosmarinic acid on sepsis-induced DNA damage in the liver of Wistar albino rats

Frontiers in Genetics ◽

10.3389/conf.fgene.2015.01.00020 ◽

2015 ◽

Vol 6 ◽

Author(s):

Goktas Hatice ◽

Bacanli Merve ◽

Aydin Sevtap ◽

Taner Gokce ◽

Sahin Tolga ◽

...

Keyword(s):

Dna Damage ◽

Rosmarinic Acid ◽

Albino Rats ◽

Protective Effects ◽

Wistar Albino Rats

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Protective effects of erdosteine on rotenone-induced oxidant injury in liver tissue

Toxicology and Industrial Health ◽

10.1191/0748233704th208oa ◽

2004 ◽

Vol 20 (6-10) ◽

pp. 141-147 ◽

Cited By ~ 20

Author(s):

Alpaslan Terzi ◽

Mustafa Iraz ◽

Semsettin Sahin ◽

Atilla Ilhan ◽

Nuri Idiz ◽

...

Keyword(s):

Lipid Peroxidation ◽

Liver Tissue ◽

Antioxidant Properties ◽

Albino Rats ◽

Protective Agent ◽

Protective Effects ◽

Oxidant Injury ◽

Sod Activity ◽

Significant Difference ◽

Wistar Albino Rats

Rotenone, an insecticide of botanical origin, causes toxicity through inhibition of complex I of the respiratory chain in mitochondria. This study was undertaken to determine whether rotenone-induced liver oxidant injury is prevented by erdosteine, a mucolytic agent showing antioxidant properties. There were four groups of Male Wistar Albino rats: group one was untreated as control; the other groups were treated with erdosteine (50 mg/kg per day, orally), rotenone (2.5 mg/mL once and 1 mL/kg per day for 60 days, i.p.) or rotenone plus erdosteine, respectively. Rotenone treatment without erdosteine increased xanthine oxidase (XO) enzyme activity and also increased lipid peroxidation in liver tissue P < 0.05). The rats treated with rotenone plus erdosteine produced a significant decrease in lipid peroxidation and XO activities in comparison with rotenone group PB / 0.05). Erdosteine treatment with rotenone led to an increase in catalase (CAT) and superoxide dismutase (SOD) activities in comparison with the rotenone group PB / 0.05). There was no significant difference in nitric oxide (NO) level between groups. There were negative correlations between CAT activity and malondialdehyde (MDA) level (r= -0.934, P <0.05) with between CAT and SOD activities (r= -0.714, P <0.05), and a positive correlation between SOD activity and MDA level (r= 0.828, P <0.05) in rotenone group. In the rotenone plus erdosteine group, there was a negative correlation between XO activity and NO level in liver tissue (r= -0.833, P -0.05). In the light of these findings, erdosteine may be a protective agent for rotenone-induced liver oxidative injury in rats.

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A Study of Position Habits Induced by Reward and “Frustration”

Quarterly Journal of Experimental Psychology ◽

10.1080/17470215108416790 ◽

1951 ◽

Vol 3 (4) ◽

pp. 151-156 ◽

Cited By ~ 4

Author(s):

Roger W. Russell ◽

Robert G. F. Pretty

Keyword(s):

Research Design ◽

Experimental Situation ◽

Central Element ◽

The Other ◽

Albino Rats ◽

Preliminary Training ◽

The Third ◽

Training Development ◽

Wistar Albino Rats ◽

Learning Principles

Predictions from Maier's theory of “frustration”-instigated behaviour have been tested in an experimental situation differing significantly from that in which the theory was propounded yet containing the central element of “frustration”—the insoluble problem. A water discrimination unit was employed in which the performance of rats would be observed during attacks on insoluble problems, position problems or symbol problems. Two groups, each containing ten Wistar albino rats, served as subjects. The research design consisted of the following phases: preliminary training, development of position responses, exposure to a symbol-reward problem with 50 per cent, punishment and exposure to a symbol-reward problem with 100 per cent, punishment. The design differed for the two groups only at the phase in which the position responses were established. During this phase one group was exposed to a position-reward problem and the other to an insoluble problem. Position responses were established as frequently under position-“frustration” (position stereotypes) as under position-reward (position habits) conditions. Position stereotypes were more rigid—more resistant to extinction—than position habits under conditions of 50 per cent, punishment. Position stereotypes were as readily extinguished under 100 per cent, punishment as were position habits under 30 per cent, punishment. The first two observations conform to predictions made from Maier's theory. The third does not. That is to say, not all situations containing the basic elements of “frustration” give rise to stereotyped behaviour patterns which are as rigid or “fixated” as Maier's theory would predict. It is a reasonable hypothesis that the characteristics of stereotyped responses established in certain “frustration” situations may be described adequately in terms of conventional learning principles without the necessity of resorting to a distinction between “goal-motivated” and “frustration-instigated” behaviour.

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Protective effects of tempol in an experimental ovarian ischemia–reperfusion injury model in female Wistar albino rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2016-0309 ◽

2017 ◽

Vol 95 (7) ◽

pp. 861-865 ◽

Cited By ~ 9

Author(s):

Neslihan Pınar ◽

Oya Soylu Karapınar ◽

Oğuzhan Özcan ◽

Esin Atik Doğan ◽

Suphi Bayraktar

Keyword(s):

Sham Group ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Stress Index ◽

Albino Rats ◽

Protective Effects ◽

Group V ◽

Group Iii ◽

Group I ◽

Wistar Albino Rats

The aim of this study was to investigate the antioxidant effects of tempol on ovarian ischemia–reperfusion (I/R) injury in rats. Forty female Wistar albino rats were randomly divided into 5 groups: Group I, sham; Group II, ischemia (I); Group III, I/R; Group IV, I/R + tempol 30 mg/kg i.p; Group V, I/R + tempol 50 mg/kg i.p. Oxidative stress index (OSI) was significantly higher in the ischemia group and the I/R group than in the sham group. Catalase levels were significantly lower in the I/R group than in the I/R + tempol 30 mg/kg i.p. and the I/R + tempol 50 mg/kg i.p. groups. Glutathione peroxidase levels were lower in the I/R group than in the I/R + tempol 30 mg/kg i.p. and the I/R + tempol 50 mg/kg i.p. groups. MDA levels were significantly lower in the I/R + tempol 30 mg/kg i.p. group and the I/R + tempol 50 mg/kg i.p. group than in the I/R group. The levels of the histopathological parameters were significantly decreased in the I/R + tempol 50 mg/kg i.p. group compared with the I/R group. Tempol can be used for reducing ovarian I/R injury.

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Antihyperlipidemic and Biochemical Activities of Mcy Protein in Streptozotocin Induced Diabetic Rats

Cellular Physiology and Biochemistry ◽

10.1159/000373954 ◽

2015 ◽

Vol 35 (4) ◽

pp. 1326-1334 ◽

Cited By ~ 7

Author(s):

Saritha Marella ◽

Dilip Rajasekhar Maddirela ◽

Kameswara Rao Badri ◽

Malaka Venkateshwarulu Jyothi Kumar ◽

Apparao Chippada

Keyword(s):

Blood Glucose ◽

Fasting Blood Glucose ◽

Diabetic Rats ◽

Albino Rats ◽

Protective Effects ◽

Tissue Lipid ◽

Lipid Levels ◽

Liver And Kidney ◽

Streptozotocin Induced Diabetes ◽

Wistar Albino Rats

Background: This study was aimed to evaluate the protective effects of a novel anti-hyperglycemic “Mcy protein” isolated from the fruits of Momordica cymbalaria in streptozotocin induced- diabetes rat model. Materials and Methods: Wild type and Streptozotocin induced diabetic male wistar albino rats were either treated with single intraperitoneal injection of 2.5 mg Mcy protein/kg body weight or acetate buffer daily for 30 days. Fasting blood glucose and, serum and tissue lipid levels were measured along with biochemical analysis for hepatic and renal function tests. Results: Mcy protein significantly reduced the fasting blood glucose and, serum as well as tissue lipid levels (p<0.05), besides normalizing the levels of liver and kidney function markers in the treated diabetic rats when compared to the diabetic controls. Our studies also showed the pancreatic islet regeneration in Mcy treated rats. Conclusion: Mcy protein can alleviate hyperlipidemia and help manage diabetes by stimulating insulin secretion without evident toxic effects on liver and kidney.

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Anti-Inflammatory Effects of Melatonin and 5-Methoxytryptophol on Lipopolysaccharide-Induced Acute Pulpitis in Rats

BioMed Research International ◽

10.1155/2021/8884041 ◽

2021 ◽

Vol 2021 ◽

pp. 1-8

Author(s):

Fatma Kermeoğlu ◽

Umut Aksoy ◽

Abdullah Sebai ◽

Gökçe Savtekin ◽

Hanife Özkayalar ◽

...

Keyword(s):

Therapeutic Strategy ◽

Oral Disease ◽

Control Group ◽

Albino Rats ◽

Filling Material ◽

Protective Effects ◽

Pulp Tissue ◽

Tnf Α ◽

Wistar Albino Rats ◽

Temporary Filling

Aim. The aim of this study was to investigate the possible therapeutic impacts of two pineal hormones, melatonin and 5-methoxytryptophol (5-MTX), in a rat model of acute pulpitis by analyzing biochemical and histopathological parameters. Methods. This research was done using 32 male and female Wistar albino rats with weight between 200 and 250 g. The rats were randomly divided into four groups: a control group (rats without any treatment), acute pulpitis (AP) group, AP+melatonin group, and AP+5-MTX group. In the AP-induced groups, the crowns of the upper left incisors were removed horizontally. Lipopolysaccharide solution was applied to the exposed pulp tissue before the canal orifices were sealed with a temporary filling material. Melatonin (10 mg/kg) and 5-MTX (5 mg/kg) were administered intraperitoneally. The rats were sacrificed 24 hours after pulp injury, and trunk blood and pulp samples were collected. The concentrations of TNF-α, IL-1β, MMP-1, and MMP-2 in sera and pulp samples were determined using ELISA assay kits. Results. TNF-α, IL-1β, MMP-1, and MMP-2 levels in the serum and pulp tissues were considerably higher in the AP group than the control group ( p < 0.01 ‐ 0.001 ). In the AP+melatonin and AP+5-MTX groups, TNF-α, IL-1β, MMP-1, and MMP-2 levels in the serum and pulp tissues were significantly lower than in the AP group ( p < 0.05 ‐ 0.001 ). Conclusions. Both melatonin and 5-MTX provided protective effects on acute pulpitis, which indicates they may be promising as a therapeutic strategy for oral disease.

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THE EFFECTIVENESS OF ELECTRO-ACUPUNCTURE IN OVARIAN ISCHEMIA REPERFUSION INJURY

Acupuncture & Electro-Therapeutics Research ◽

10.3727/036012921x16264625521781 ◽

2021 ◽

Author(s):

Seda Keskin ◽

Deha Denizhan Keskin ◽

Muhammet Değermenci ◽

Hatice Hancı ◽

Nuket Göçmen Karabekir ◽

...

Keyword(s):

Reperfusion Injury ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Reproductive Age ◽

Albino Rats ◽

Sham Operation ◽

Adnexal Torsion ◽

Fourth Group ◽

The Third ◽

Wistar Albino Rats

Electro-acupuncture is widely used in gynecology. Adnexal torsion is an important threat to ovarian reserves in women of reproductive age. This is the first study to investigate whether electro-acupuncture is beneficial in protecting ovarian reserves in case of adnexal torsion resulting in reperfusion injury. Thirty-two female Wistar Albino rats were randomized into four groups: the first group—sham operation, the second group—torsion/detorsion model, the third group—pre-acupuncture + torsion/detorsion + post-acupuncture, and the fourth group—torsion/detorsion + post-acupuncture. The acupoints used were CV4 and bilateral SP6, Ex-CA1, Kid3, and ST36. In the third group, the acupoints were needled for two weeks before torsion, continuing for a further two weeks after torsion. In the fourth group, needling began after torsion and was maintained for two weeks. Both histological and biochemical parameters indicating ovarian reserves showed that electro-acupuncture applied to the above points exhibited an ameliorating effect on ovaries injured during ischemia/reperfusion. Electro-acupuncture may be capable of protecting against and preventing ischemia/reperfusion injury in case of ovarian torsion.

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