Development of a rapid assay methodology for antimicrobial susceptibility testing of Staphylococcus aureus

2008 ◽  
Vol 62 (3) ◽  
pp. 306-316 ◽  
Author(s):  
Janne O. Koskinen ◽  
Teppo Stenholm ◽  
Jonne Vaarno ◽  
Jori Soukka ◽  
Niko J. Meltola ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Rapid Assay

scholarly journals The Frequency of Methicillin-Resistant Staphylococcus aureus and Coagulase Gene Polymorphism in Egypt

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Hend M. Abdulghany ◽  
Rasha M. Khairy
Keyword(s):  
Staphylococcus Aureus ◽  
Gene Polymorphism ◽  
Antimicrobial Susceptibility ◽  
Antimicrobial Agents ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Methicillin Resistant ◽  
Microbiological Methods ◽  
Rflp Typing

The current study aimed to use Coagulase gene polymorphism to identify methicillin-resistant Staphylococcus aureus (MRSA) subtypes isolated from nasal carriers in Minia governorate, Egypt, evaluate the efficiency of these methods in discriminating variable strains, and compare these subtypes with antibiotypes. A total of 400 specimens were collected from nasal carriers in Minia governorate, Egypt, between March 2012 and April 2013. Fifty-eight strains (14.5%) were isolated and identified by standard microbiological methods as MRSA. The identified isolates were tested by Coagulase gene RFLP typing. Out of 58 MRSA isolates 15 coa types were classified, and the amplification products showed multiple bands (1, 2, 3, 4, 5, and 8 bands). Coagulase gene PCR-RFLPs exhibited 10 patterns that ranged from 1 to 8 fragments with AluI digestion. Antimicrobial susceptibility testing with a panel of 8 antimicrobial agents showed 6 different antibiotypes. Antibiotype 1 was the most common phenotype with 82.7%. The results have demonstrated that many new variants of the coa gene are present in Minia, Egypt, different from those reported in the previous studies. So surveillance of MRSA should be continued.

scholarly journals Methicillin-Resistant Staphylococcus Aureus (mrsa) as a Cause of Nosocomial Wound Infections

2010 ◽  
Vol 10 (1) ◽  
pp. 32-37 ◽  
Author(s):  
Maida Šiširak ◽  
Amra Zvizdić ◽  
Mirsada Hukić
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Diffusion Method ◽  
Wound Infections ◽  
Common Cause ◽  
Methicillin Resistant ◽  
Mrsa Infection

Postoperative wound infections represent about 16% of hospital-acquired infections. Staphylococcus aureus is the most common cause of nosocomial wound infections. Increased frequency of Methicillin-re- sistant Staphylococcus aureus (MRSA) in hospitalized patients and possibility of vancomycin resistance requires permanent control of MRSA spread in the hospital.The purpose of this study was to analyse the frequency of Methicillin-resistant Staphylococcus aureus (MRSA) in the swabs taken from the surgical wounds, the presence of MRSA infection in surgical departments and to examine antimicrobial susceptibility of MRSA isolates.Wound swabs were examined from January 2006 to December 2008. The isolates were identified by conventional methods. Antimicrobial susceptibility testing was performed by Kirby-Bauer disc-diffusion method as per NCCLS guidelines.A total of 5755 wound swabs were examined: 938 (16,3%) swabs were sterile and 4817 (83,7%) were positive. Staphylococcus aureus was isolated in 1050 (22,0%) swabs and it was the most common cause of wound infections. MRSA was isolated from 12,4% samples in 2006, from 6,7% samples in 2007 and from 3,7% samples during 2008. Wound infections caused by MRSA dominated in the department of plastic surgery (24,4%) and in the department of orthopaedic surgery (24,1%). Antimicrobial susceptibility testing showed that 73% of MRSA isolates were with the same antibiotic sensitivity pattern (antibiotyp)- sensitive only to vancomycin, tetracycline, fucid acid and trimethoprim/sulfamethoxasole.Our results show decreasing of MRSA infection in the surgical wards. These results appear to be maintained with strategies for preventing nosocomial infection: permanent education, strong application of protocols and urging the implementation of strict infection control policy.

scholarly journals Nasal carriage rate and antimicrobial resistance pattern of Staphylococcus aureus among the food handlers in Canton Sarajevo, Bosnia and Herzegovina

2020 ◽  
Author(s):  
Sabina Šegalo ◽  
Daniel Maestro ◽  
Zarema Obradović ◽  
Anes Jogunčić
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Bosnia And Herzegovina ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Nasal Carriage ◽  
Antimicrobial Susceptibility Testing ◽  
Food Handlers ◽  
Carriage Rate

Introduction: The nasals and hand carriage of Staphylococcus aureus in food handlers (FHs) represent a significant source of Staphylococcal food contamination and food poisoning. Antimicrobial resistance (AMR) is a microorganism’s ability to resist the action of one or more antimicrobial agents. S. aureus has demonstrated the ability to rapidly respond to each new antimicrobial with the development of a resistance mechanism. The aim of the study was to assess the prevalence of nasal carriage rate and AMR pattern of isolated strains S. aureus among FHs in Canton Sarajevo, Bosnia and Herzegovina. Methods: The retrospective study included laboratory results of 11.139 tested subjects between January 2014 and December 2018. The study was conducted in the laboratory of the Institute of Public Health of the Federation of Bosnia and Herzegovina in Sarajevo. Samples of nasal swabs were collected from FHs, employees in companies located in Canton Sarajevo, during sanitary surveillance prescribed by applicable legal standards. S. aureus isolates were identified according to conventional microbiological methods and antimicrobial susceptibility testing was performed by the agar disk diffusion method according to the European Committee on Antimicrobial Susceptibility Testing; 2013 standard. Results: Among the 11.138 subjects, 792 (7.1%) were carriers of S. aureus. Isolated strains were tested on eight different antibiotics, and the resistance to penicillin, ampicillin, and amoxicillin was 788 (99.5%), 776 (97.9%), and 752 (94.9%), retrospectively. In total, 86.36% of isolated strains were multidrug-resistant. Conclusions: The low percentage of S. aureus carriers indicates that preventive measures of carrier control are being actively implemented within the legally prescribed measures. The emergence of numerous isolated strains with multidrug-resistance characteristics is a significant public health problem and consequently limits the range of antibiotics available for therapeutic purposes. The results of this research indicate that AMR has increased in Sarajevo Canton and it is following the trend of global growth.

scholarly journals Evaluation of the antimicrobial susceptibility testing process in clinical microbiology laboratories at Niamey, Niger

2021 ◽  
Vol 22 (4) ◽  
pp. 448-456
Author(s):  
H. Idrissa ◽  
O. Abdoulaye ◽  
A. Yacouba ◽  
D. Alhousseini Maiga ◽  
H. Moumouni Sambo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Risk Assessment ◽  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Clinical Microbiology ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Critical Control Points ◽  
Criticality Index ◽  
Reference Strains

Background: Risk assessment is the means of identifying and evaluating potential errors or problems that may occur in testing process. The aim of this study was to perform risk assessment of antimicrobial susceptibility testing (AST) process in clinical microbiology laboratories of Niamey, Niger Republic.Methodology: We conducted a descriptive cross-sectional study from October 1 to December 31, 2019, to evaluate AST performance in seven clinical microbiology laboratories at Niamey, the capital city of Niger republic. The evaluation focused on the determination of the criticality index (CI) of each critical point (frequency of occurrence of anomalies, severity of the process anomaly, and detectability of the anomaly during the process) in the AST process and the performance of the AST through an observation sheet using two reference strains; Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213.Results: The criticality index (CI) was greater than 6 for most of the critical points related to material, medium, equipment, method and labour for the AST process in all the laboratories. A range of 18-100% errors on the inhibition zone diameters of the reference strains were observed. Major and/or minor categorization (Sensitive S, Intermediate I and Resistance R) discrepancies were found at all the laboratories for either one or both reference strains. The antibiotics most affected by the S/I/R discrepancies were trimethoprim (100%), vancomycin (100%), amoxicillin (80%) and amoxicillin + clavulanic acid (70%).Conclusion: This study showed a deficiency in the control of critical control points that impacts the performance of the AST reported by the laboratories in Niger. Corrective actions are needed to improve the performance of AST in clinical microbiology laboratories in Niger.   French title: Evaluation du processus de réalisation de l’antibiogramme dans les laboratoires d’analyses de biologie médicale de la ville de Niamey, Niger Contexte: L'évaluation des risques est le moyen d'identifier et d'évaluer les erreurs ou les problèmes potentiels qui peuvent survenir dans le processus de test. L'objectif de cette étude était de réaliser une évaluation des risques du processus d'antibiogramme (ABG) dans les laboratoires de microbiologie clinique de Niamey, en République du Niger.Méthodologie: Nous avons mené une étude transversale descriptive du 1er octobre au 31 décembre 2019 pour évaluer la performance des ABG dans sept laboratoires de microbiologie clinique à Niamey, capitale de la république du Niger. L'évaluation a porté sur la détermination de l'indice de criticité (IC) de chaque point critique (fréquence d'apparition des anomalies, gravité de l'anomalie du processus et détectabilité de l'anomalie au cours du processus) dans le processus et la performance des AGB à travers une fiche d'observation en utilisant deux souches de référence; Escherichia coli ATCC 25922 et Staphylococcus aureus ATCC 29213.Résultats: L'indice de criticité était supérieur à 6 pour la plupart des points critiques liés au matériel, au milieu, à l'équipement, à la méthode et à la main-d'oeuvre pour le processus AST dans tous les laboratoires. Une fourchette d'erreurs de 18 à 100% sur les diamètres des zones d'inhibition des souches de référence a été observée. Des écarts de catégorisation majeurs et/ou mineurs (Sensible: S, Intermédiaire: I et Résistance: R) ont été constatés dans tous les laboratoires pour l'une ou les deux souches de référence. Les  antibiotiques les plus touchés par les écarts S/I/R étaient la triméthoprime (100%), la vancomycine (100%), l'amoxicilline (80%) et l'amoxicilline + acide clavulanique (70%).Conclusion: Cette étude a montré une déficience dans le contrôle des points de contrôle critiques qui a un impact sur la performance de l'antibiogramme rapportée par les laboratoires au Niger. Des actions correctives sont nécessaires pour améliorer la performance des ABG dans les laboratoires de microbiologie clinique au Niger.

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