Acinetobacter pittii and Acinetobacter nosocomialis among clinical isolates of the Acinetobacter calcoaceticus-baumannii complex in Sichuan, China

2013 ◽  
Vol 76 (3) ◽  
pp. 392-395 ◽  
Author(s):  
Xiaohui Wang ◽  
Tao Chen ◽  
Rujia Yu ◽  
Xiaojü Lü ◽  
Zhiyong Zong
Keyword(s):  
Acinetobacter Calcoaceticus ◽  
Clinical Isolates ◽  
Acinetobacter Pittii

scholarly journals Comparison of Genospecies and Antimicrobial Resistance Profiles of Isolates in the Acinetobacter calcoaceticus-Acinetobacter baumannii Complex from Various Clinical Specimens

2012 ◽  
Vol 56 (12) ◽  
pp. 6267-6271 ◽  
Author(s):  
Ni Tien ◽  
Bang-Jau You ◽  
Hui-Lan Chang ◽  
Hsiu-Shen Lin ◽  
Chin-Yi Lee ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Acinetobacter Baumannii ◽  
Restriction Analysis ◽  
Acinetobacter Calcoaceticus ◽  
Its Region ◽  
Clinical Isolates ◽  
23S Rrna ◽  
Rrna Gene ◽  
Genotype 3 ◽  
Content Type

ABSTRACTThis study was conducted to compare the prevalences of antimicrobial resistance profiles of clinical isolates in theAcinetobacter calcoaceticus-Acinetobacter baumanniicomplex from sterile and nonsterile sites and to further study the relationship of antimicrobial resistance profiles and genospecies by amplified rRNA gene restriction analysis (ARDRA). A total of 1,381 isolates were tested with 12 different antibiotics to show their antimicrobial susceptibility profiles. A total of 205 clinical isolates were further analyzed by ARDRA of the intergenic spacer (ITS) region of the 16S-23S rRNA gene. It was found that the overall percentage of isolates from nonsterile sites (urine, sputum, pus, or catheter tip) that were resistant to the 12 antibiotics tested was significantly higher than that of isolates from sterile sites (cerebrospinal fluid [CSF], ascites fluid, and bloodstream) (46% versus 22%;P< 0.05). After ARDRA, it was found that 97% of the 62 isolates resistant to all antibiotics tested were theA. baumanniigenospecies, which was identified in only 31% of the isolates susceptible to all antibiotics tested. More genospecies diversity was identified in the isolates susceptible to all antibiotics tested, including genospecies of 13TU (34%), genotype 3 (29%), andA. calcoaceticus(5%). Furthermore, as 91% (10/11) of the isolates from CSF were susceptible to all antibiotics tested, theA. calcoaceticus-A. baumanniicomplex isolates with multidrug resistance could be less invasive than the more susceptible isolates. This study also indicated current emergence of carbapenem-, fluoroquinolone-, aminoglycoside-, and cephalosporin-resistantA. calcoaceticus-A. baumanniicomplex isolates in Taiwan.

scholarly journals Rapid identification of Acinetobacter baumannii, Acinetobacter nosocomialis and Acinetobacter pittii with a multiplex PCR assay

2014 ◽  
Vol 63 (9) ◽  
pp. 1154-1159 ◽  
Author(s):  
Te-Li Chen ◽  
Yi-Tzu Lee ◽  
Shu-Chen Kuo ◽  
Su-Pen Yang ◽  
Chang-Phone Fung ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Multiplex Pcr ◽  
Acinetobacter Calcoaceticus ◽  
Rapid Identification ◽  
23S Rrna ◽  
Pcr Assay ◽  
Complex Species ◽  
Multiplex Pcr Assay ◽  
Acinetobacter Pittii ◽  
Reference Strains

Acinetobacter baumannii, Acinetobacter nosocomialis and Acinetobacter pittii are clinically relevant members of the Acinetobacter calcoaceticus–A. baumannii (Acb) complex and important nosocomial pathogens. These three species are genetically closely related and phenotypically similar; however, they differ in their epidemiology, antibiotic resistance and pathogenicity. In this study, we investigated the use of a multiplex PCR-based assay designed to detect internal fragments of the 16S–23S rRNA intergenic region and the gyrB and recA genes. The assay was capable of differentiating A. baumannii, A. nosocomialis and A. pittii in a reliable manner. In 23 different reference strains and 89 clinical isolates of Acinetobacter species, the assay accurately identified clinically relevant Acb complex species except those ‘between 1 and 3’ or ‘close to 13TU’. None of the non-Acb complex species was misidentified. In an analysis of 1034 positive blood cultures, the assay had a sensitivity of 92.4 % and specificity of 98.2 % for Acb complex identification. Our results show that a single multiplex PCR assay can reliably differentiate clinically relevant Acb complex species. Thus, this method may be used to better understand the clinical differences between infections caused by these species.

scholarly journals Emergence of NDM-1 and OXA-72 producing Acinetobacter pittii clinical isolates in Lebanon

2016 ◽  
Vol 12 ◽  
pp. 43-44 ◽  
Author(s):  
A. Al Atrouni ◽  
M.-L. Joly-Guillou ◽  
M. Hamze ◽  
M. Kempf
Keyword(s):  
Clinical Isolates ◽  
Acinetobacter Pittii

scholarly journals Expression of the Resistance-Nodulation-Cell Division Pump AdeIJK in Acinetobacter baumannii Is Regulated by AdeN, a TetR-Type Regulator

2012 ◽  
Vol 56 (5) ◽  
pp. 2504-2510 ◽  
Author(s):  
Nicolas Rosenfeld ◽  
Christiane Bouchier ◽  
Patrice Courvalin ◽  
Bruno Périchon
Keyword(s):  
Acinetobacter Baumannii ◽  
Acinetobacter Calcoaceticus ◽  
Fold Increase ◽  
Intrinsic Resistance ◽  
Content Type ◽  
Reverse Transcription Pcr ◽  
Drug Classes ◽  
Resistance Nodulation Division ◽  
Acinetobacter Pittii ◽  
One Step

ABSTRACTResistance-nodulation-division efflux system AdeIJK contributes to intrinsic resistance to various drug classes inAcinetobacter baumannii. By whole-genome sequencing, we have identified in clinical isolate BM4587 theadeNgene, located 813 kbp upstream fromadeIJK, which encodes a TetR transcriptional regulator. In one-step mutant BM4666 overexpressingadeIJK, the deletion of cytosine 582 (C582) in the 3′ portion of this gene was responsible for a frameshift mutation resulting in the deletion of the seven C-terminal residues.trans-Complementation of this BM4587 derivative with a plasmid expressingadeNrestored antibiotic susceptibility to the host associated with the loss ofadeJoverexpression. The inactivation ofadeNin BM4587 led to a diminished susceptibility to antibiotics that are substrates for AdeIJK and to a 5-fold increase inadeJexpression. Taken together, these results indicate that AdeN represses AdeIJK expression. Quantitative reverse transcription-PCR (qRT-PCR) demonstrated that AdeN is constitutively expressed in BM4587 and does not regulate its own expression. Deletion of cytosine 582 and a 394-bp deletion of the 3′ part ofadeNwere found in independent one-stepadeIJK-overexpressing mutants selected from clinical isolates BM4667 and BM4651, respectively. The corresponding alterations were located in the α9 helix, which is known to be involved in dimerization, a process essential for the activity of TetR regulators. TheadeNgene was detected in all of the 30A. baumanniistrains tested and inAcinetobacter calcoaceticus,Acinetobacter nosocomialis, andAcinetobacter pittii.

scholarly journals Identification of 50 Class D β-Lactamases and 65 Acinetobacter-Derived Cephalosporinases in Acinetobacter spp.

2013 ◽  
Vol 58 (2) ◽  
pp. 936-949 ◽  
Author(s):  
Bruno Périchon ◽  
Sylvie Goussard ◽  
Violaine Walewski ◽  
Lenka Krizova ◽  
Gustavo Cerqueira ◽  
...  
Keyword(s):  
Phylogenetic Tree ◽  
Phylogenetic Trees ◽  
Acinetobacter Calcoaceticus ◽  
Phylogenetic Tree Analysis ◽  
Content Type ◽  
New Class ◽  
Class D ◽  
Acinetobacter Pittii ◽  
Acinetobacter Spp ◽  
Hydrolysis Of

ABSTRACTWhole-genome sequencing of a collection of 103Acinetobacterstrains belonging to 22 validly named species and another 16 putative species allowed detection of genes for 50 new class D β-lactamases and 65 newAcinetobacter-derived cephalosporinases (ADC). All oxacillinases (OXA) contained the three typical motifs of class D β-lactamases, STFK, (F/Y)GN, and K(S/T)G. The phylogenetic tree drawn from the OXA sequences led to an increase in the number of OXA groups from 7 to 18. The topologies of the OXA and RpoB phylogenetic trees were similar, supporting the ancient acquisition ofblaOXAgenes byAcinetobacterspecies. The class D β-lactamase genes appeared to be intrinsic to several species, such asAcinetobacter baumannii,Acinetobacter pittii,Acinetobacter calcoaceticus, andAcinetobacter lwoffii. NeitherblaOXA-40/143- norblaOXA-58-like genes were detected, and their origin remains therefore unknown. The phylogenetic tree analysis based on the alignment of the sequences deduced fromblaADCrevealed five main clusters, one containing ADC belonging to species closely related toA. baumanniiand the others composed of cephalosporinases from the remaining species. No indication ofblaOXAorblaADCtransfer was observed between distantly related species, except forblaOXA-279, possibly transferred fromAcinetobactergenomic species 6 toAcinetobacter parvus. Analysis of β-lactam susceptibility of seven strains harboring new oxacillinases and cloning of the corresponding genes inEscherichia coliand in a susceptibleA. baumanniistrain indicated very weak hydrolysis of carbapenems. Overall, this study reveals a large pool of β-lactamases in differentAcinetobacterspp., potentially transferable to pathogenic strains of the genus.

scholarly journals Complete Genome Sequence of Acinetobacter pittii BHS4, Isolated from Air-Conditioning Condensate in Hong Kong

2021 ◽  
Vol 10 (42) ◽  
Author(s):  
B. H. Saunders ◽  
G. K. K. Lai ◽  
S. D. J. Griffin ◽  
F. C. C. Leung
Keyword(s):  
Hong Kong ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Air Conditioning ◽  
Gc Content ◽  
Acinetobacter Calcoaceticus ◽  
Content Type ◽  
Acinetobacter Pittii ◽  
Hospital Acquired

Acinetobacter pittii is widespread in the environment, and the Acinetobacter calcoaceticus -baumannii complex, to which it belongs, is a major cause of hospital-acquired pneumonia and bacteremia. A. pitti BHS4 was isolated from an air-conditioning unit in Hong Kong and its complete genome sequence (3,901,980 bp; GC content, 38.79%) established through hybrid assembly.

A novel enzyme synthesized by Acinetobacter sp. SM04 is responsible for zearalenone biodegradation

2021 ◽  
Author(s):  
Yuqian Tang ◽  
Chendi Liu ◽  
Jiguo Yang ◽  
Xian Peng
Keyword(s):  
Fusarium Species ◽  
Acinetobacter Calcoaceticus ◽  
Toxic Metabolite ◽  
Gene Encoding ◽  
E Coli ◽  
Acinetobacter Pittii ◽  
Related Enzyme ◽  
Acinetobacter Sp ◽  
Proliferation Assays ◽  
Mcf 7

Abstract Zearalenone (ZEA), a non-steroidal estrogenic mycotoxin produced by multiple Fusarium species, contaminates cereals and threatens the health of both humans and animals by inducing hepatotoxicity, immunotoxicity, and genotoxicity. A new alkali tolerant enzyme named Ase, capable of degrading ZEA without H2O2, was derived from Acinetobacter sp. SM04 in this study. The Ase gene shares 97% sequence identity with hypothetical proteins from Acinetobacter pittii strain WCHAP 100004 and YMC 2010/8/T346 and Acinetobacter calcoaceticus PHEA-2, respectively. Based on the Acinetobacter genus database, the gene encoding Ase was cloned and extracellularly expressed in E. coli BL21. After degrading 88.4% of ZEA (20 μg/mL), it was confirmed through MCF-7 cell proliferation assays that Ase can transform ZEA into a non-estrogenic toxic metabolite. Recombinant Ase (molecular weight: 28 kDa), produced by E. coli BL21/pET32a(+)-His-Ase, was identified as an oxygen-utilizing and cytochrome-related enzyme with optimal activity at 60 °C and pH 9.0.

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