scholarly journals Metagenomic data on the composition of bacterial communities in lake environment sediments for fish farming by next generation Illumina sequencing

Data in Brief ◽  
2020 ◽  
Vol 32 ◽  
pp. 106228
Author(s):  
María Custodio ◽  
Alberto Ordinola-Zapata ◽  
Ciro Espinoza ◽  
Enedia Vieyra-Peña ◽  
Richard Peñaloza ◽  
...  
Keyword(s):  
Illumina Sequencing ◽  
Bacterial Communities ◽  
Fish Farming ◽  
Metagenomic Data ◽  
Next Generation

scholarly journals Metagenomic data of free cyanide and thiocyanate degrading bacterial communities

Data in Brief ◽  
2017 ◽  
Vol 13 ◽  
pp. 738-741 ◽  
Author(s):  
Lukhanyo Mekuto ◽  
Seteno K.O. Ntwampe ◽  
John B.N. Mudumbi ◽  
Enoch A. Akinpelu ◽  
Maxwell Mewa-Ngongang
Keyword(s):  
Bacterial Communities ◽  
Metagenomic Data ◽  
Free Cyanide

scholarly journals DNA Extraction and Host Depletion Methods Significantly Impact and Potentially Bias Bacterial Detection in a Biological Fluid

mSystems ◽  
2021 ◽  
Author(s):  
Erika Ganda ◽  
Kristen L. Beck ◽  
Niina Haiminen ◽  
Justin D. Silverman ◽  
Ban Kawas ◽  
...  
Keyword(s):  
Food Safety ◽  
Dna Extraction ◽  
Bacterial Communities ◽  
Biological Fluid ◽  
Genetic Material ◽  
Next Generation ◽  
Microbial Composition ◽  
Chemical Methods ◽  
Bacterial Detection ◽  
Do So

Tracking the bacterial communities present in our food has the potential to inform food safety and product origin. To do so, the entire genetic material present in a sample is extracted using chemical methods or commercially available kits and sequenced using next-generation platforms to provide a snapshot of the microbial composition.

scholarly journals Exploring changes in bacterial communities to assess the influence of fish farming on marine sediments

Aquaculture ◽  
2019 ◽  
Vol 506 ◽  
pp. 459-464 ◽  
Author(s):  
Esther Rubio-Portillo ◽  
Adriana Villamor ◽  
Victoria Fernandez-Gonzalez ◽  
Josefa Antón ◽  
Pablo Sanchez-Jerez
Keyword(s):  
Marine Sediments ◽  
Bacterial Communities ◽  
Fish Farming

BPHL SARS-CoV-2 Tiled Amplicon Illumina Sequencing v2

2021 ◽  
Author(s):  
Jason Blanton
Keyword(s):  
Public Health ◽  
Next Generation Sequencing ◽  
Illumina Sequencing ◽  
Library Preparation ◽  
Next Generation ◽  
Florida Department ◽  
Wet Lab ◽  
Generation Sequencing

This protocol details the Florida Department of Health's Bureau of Public Health Laboratories' (BPHL) wet lab portion of our SARS-CoV-2 next generation sequencing workflow. The method is a tiled amplicon approach using ARTIC V3 primers. The amplicon generation was adapted from the Matteson protocol1. The library preparation is Illumina NexteraXT. Library pooling and normalization were adapted from the Gohl protocol3. This protocol is for loading a MiSeq, but we have had equal success running on iSeqs and NextSeqs as well. Up to 96 libraries can be run on a MiSeq and up to 384 on a NextSeq.

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