The glucagon-like peptide 1 receptor agonist Exendin-4 induces tenogenesis in human mesenchymal stem cells

2021 ◽  
Author(s):  
Sama Abdulmalik ◽  
Daisy Ramos ◽  
Swetha Rudraiah ◽  
Yeshavanth Kumar Banasavadi-Siddegowda ◽  
Sangamesh G. Kumbar
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Receptor Agonist ◽  
Human Mesenchymal Stem Cells ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1

De Novo Arteriovenous Malformation Growth Secondary to Implantation of Genetically Modified Allogeneic Mesenchymal Stem Cells in the Brain

Neurosurgery ◽  
2015 ◽  
Vol 78 (4) ◽  
pp. E596-E600 ◽  
Author(s):  
Makoto Nakamura ◽  
Amir Samii ◽  
Josef M. Lang ◽  
Friedrich Götz ◽  
Madjid Samii ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Arteriovenous Malformation ◽  
De Novo ◽  
Genetically Modified ◽  
Biological Drug ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1 ◽  
The Brain

Abstract BACKGROUND AND IMPORTANCE: Local biological drug delivery in the brain is an innovative field of medicine that developed rapidly in recent years. Our report illustrates a unique case of de novo development of a cerebral arteriovenous malformation (AVM) after implantation of genetically modified allogeneic mesenchymal stem cells in the brain. CLINICAL PRESENTATION: A 50-year-old man was included in a prospective clinical study (study ID number CM GLP-1/01, 2007-004516-31) investigating a novel neuroprotective approach in stroke patients to prevent perihematomal neuronal damage. In this study, alginate microcapsules containing genetically modified allogeneic mesenchymal stem cells producing the neuroprotective glucagon-like peptide-1 (GLP-1) were implanted. Three years later, the patient presented with aphasia and a focal seizure due to a new left frontal intracerebral hemorrhage. Angiography revealed a de novo left frontal AVM. CONCLUSION: The development of an AVM within a period of 3 years after implantation of the glucagon-like peptide-1–secreting mesenchymal stem cells suggests a possible relationship. This case exemplifies that further investigations are necessary to assess the safety of genetically modified cell lines for local biological drug delivery in the brain.

scholarly journals Encapsulated Glucagon-Like Peptide-1-Producing Mesenchymal Stem Cells Have a Beneficial Effect on Failing Pig Hearts

2012 ◽  
Vol 1 (10) ◽  
pp. 759-769 ◽  
Author(s):  
Elizabeth J. Wright ◽  
Kelly A. Farrell ◽  
Nadim Malik ◽  
Moustapha Kassem ◽  
Andrew L. Lewis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Beneficial Effect ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1

Signaling and biological effects of glucagon-like peptide 1 on the differentiation of mesenchymal stem cells from human bone marrow

2010 ◽  
Vol 298 (3) ◽  
pp. E634-E643 ◽  
Author(s):  
C. Sanz ◽  
P. Vázquez ◽  
C. Blázquez ◽  
P. A. Barrio ◽  
M. Del M. Alvarez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Differentiation ◽  
Signaling Pathways ◽  
Cell Apoptosis ◽  
Human Bone ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1

Glucagon-like peptide 1 (GLP-1) functions as an incretin hormone with antidiabetogenic properties. However, the role of GLP-1 in human bone marrow-derived mesenchymal stem cells (hMSCs), if any, remains unknown. The effects of GLP-1 on hMSCs were tested with regard to cell proliferation, cytoprotection, and cell differentiation into adipocytes. The signaling pathways involved in these processes were also analyzed. Cells were characterized with biochemical and morphological approaches before and after being induced to differentiate into adipocytes. PCNA protein levels were used as a proliferation index, whereas cell apoptosis was studied by deprivation of fetal bovine serum. Isolated hMSCs expressed stem cell markers as well as mRNA and GLP-1 receptor protein. GLP-1 increased the proliferation of hMSCs, which decreased when they were induced to differentiate into adipocytes. This process produced biochemical and morphological changes in cells expressing PPARγ, C/EBPβ, AP2, and LPL in a time-dependent pattern. Notably, GLP-1 significantly reduced the expression of PPARγ, C/EBPβ, and LPL. These effects were exerted at least through the MEK and PKC signaling pathways. In addition, GLP-1 significantly reduced cell apoptosis. Our data indicate that, in hMSCs, GLP-1 promotes cellular proliferation and cytoprotection and prevents cell differentiation into adipocytes. These latter findings underscore the potential therapeutic role of GLP-1 in preventing the adipocyte hyperplasia associated with obesity and, additionally, could bolster the maintenance of hMSC stores by promoting the proliferation and cytoprotection of undifferentiated hMSC.

Cell-based delivery of glucagon-like peptide-1 using encapsulated mesenchymal stem cells

2012 ◽  
Vol 30 (4) ◽  
pp. 315-324 ◽  
Author(s):  
Christine Wallrapp ◽  
Eric Thoenes ◽  
Frank Thürmer ◽  
Anette Jork ◽  
Moustapha Kassem ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1
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