Red emission cysteine probe with high selectivity based on fluorescent protein chromophores and turn-on fluorescence in cell cultures

2019 ◽  
Vol 166 ◽  
pp. 350-356 ◽  
Author(s):  
Baoxing Shen ◽  
Ying Qian
Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3825
Author(s):  
Ling-Yi Shen ◽  
Xiao-Li Chen ◽  
Xian-Jiong Yang ◽  
Hong Xu ◽  
Ya-Li Huang ◽  
...  

A novel turn-on fluorescence probe L has been designed that exhibits high selectivity and sensitivity with a detection limit of 9.53 × 10−8 mol/L for the quantification of Zn2+. 1H-NMR spectroscopy and single crystal X-ray diffraction analysis revealed the unsymmetrical nature of the structure of the Schiff base probe L. An emission titration experiment in the presence of different molar fractions of Zn2+ was used to perform a Job’s plot analysis. The results showed that the stoichiometric ratio of the complex formed by L and Zn2+ was 1:1. Moreover, the molecular structure of the mononuclear Cu complex reveals one ligand L coordinates with one Cu atom in the asymmetric unit. On adding CuCl2 to the ZnCl2/L system, a Cu-Zn complex was formed and a strong quenching behavior was observed, which inferred that the Cu2+ displaced Zn2+ to coordinate with the imine nitrogen atoms and hydroxyl oxygen atoms of probe L.


2009 ◽  
Vol 18 (2) ◽  
pp. 460-466 ◽  
Author(s):  
Xiaokun Shu ◽  
Lei Wang ◽  
Leslie Colip ◽  
Karen Kallio ◽  
S. James Remington

2013 ◽  
Vol 295-298 ◽  
pp. 475-478 ◽  
Author(s):  
Zhi Xiang Han ◽  
Ming Hui Du ◽  
Guo Xi Liang ◽  
Xiang Yang Wu

Rhodamine B thiohydrazide (RBS) was firstly employed as turn-on fluorescent probe for hypochlorite in aqueous solution and living cells. It exhibits a stable response to hypochlorite from 1.0×10-6to 1.0×10-5M with a detection limit of 3.3×10-7M. The response of this probe to hypochlorite is fast and highly selective compared with other reactive oxygen species (such as.OH,1O2, H2O2) and other common anions (such as X-, ClO2-, ClO4-, NO3-, NO2-, OH-, Ac-, CO32-, SO42-).


2018 ◽  
Vol 31 (2) ◽  
pp. 80-88 ◽  
Author(s):  
Xiao-Mei Jiang ◽  
Wen-Hui Mi ◽  
Wei Zhu ◽  
Hong Yao ◽  
You-Ming Zhang ◽  
...  

2015 ◽  
Vol 25 (3) ◽  
pp. 557-561 ◽  
Author(s):  
Wei Liu ◽  
Guangyu Wu ◽  
Xiaomin Gu ◽  
Xiaosheng Yuan ◽  
Jueyong Li ◽  
...  

2016 ◽  
Vol 40 (3) ◽  
pp. 1930-1934 ◽  
Author(s):  
M. Raju ◽  
Tapasya J. Patel ◽  
Ratish R. Nair ◽  
Pabitra B. Chatterjee

Synopsis: A well-known tryptophan metabolite, xanthurenic acid, a natural non-fluorescent intermediate siderophore, showed a very selective turn-on response to K+ over other competing metal ions and the detection limit of this natural ionophore was found to be 53 nM at physiological pH.


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