A near-infrared bioprobe with aggregation-induced emission feature for in vitro photodynamic therapy

2021 ◽  
pp. 109521
Author(s):  
Liucheng Mao ◽  
Hongye Huang ◽  
Danning Hu ◽  
Haijun Ma ◽  
Mei Tian ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Near Infrared ◽  
Emission Feature ◽  
Aggregation Induced Emission

scholarly journals Photoactivated nanomotors via aggregation induced emission for enhanced phototherapy

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shoupeng Cao ◽  
Jingxin Shao ◽  
Hanglong Wu ◽  
Shidong Song ◽  
Maria Teresa De Martino ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Photodynamic Therapy ◽  
Near Infrared ◽  
Radiant Energy ◽  
Reactive Oxygen ◽  
Infrared Light ◽  
Oxygen Species ◽  
Directed Motion ◽  
Aggregation Induced Emission

AbstractAggregation-induced emission (AIE) has, since its discovery, become a valuable tool in the field of nanoscience. AIEgenic molecules, which display highly stable fluorescence in an assembled state, have applications in various biomedical fields—including photodynamic therapy. Engineering structure-inherent, AIEgenic nanomaterials with motile properties is, however, still an unexplored frontier in the evolution of this potent technology. Here, we present phototactic/phototherapeutic nanomotors where biodegradable block copolymers decorated with AIE motifs can transduce radiant energy into motion and enhance thermophoretic motility driven by an asymmetric Au nanoshell. The hybrid nanomotors can harness two photon near-infrared radiation, triggering autonomous propulsion and simultaneous phototherapeutic generation of reactive oxygen species. The potential of these nanomotors to be applied in photodynamic therapy is demonstrated in vitro, where near-infrared light directed motion and reactive oxygen species induction synergistically enhance efficacy with a high level of spatial control.

Folic Acid Functionalized Chlorin e6-Superparamagnetic Iron Oxide Nanocarriers as a Theranostic Agent for MRI-Guided Photodynamic Therapy

2021 ◽  
Vol 17 (2) ◽  
pp. 205-215
Author(s):  
Zhenbo Sun ◽  
Mingfang Luo ◽  
Jia Li ◽  
Ailing Wang ◽  
Xucheng Sun ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Folic Acid ◽  
Iron Oxide ◽  
Near Infrared ◽  
Biological Fluids ◽  
Superparamagnetic Iron Oxide ◽  
Chlorin E6 ◽  
Theranostic Agent

Imaging-guided cancer theranostic is a promising strategy for cancer diagnostic and therapeutic. Photodynamic therapy (PDT), as an approved treatment modality, is limited by the poor solubility and dispersion of photosensitizers (PS) in biological fluids. Herein, it is demonstrated that superparamagnetic iron oxide (SPIO)-based nanoparticles (SCFs), prepared by conjugated with Chlorin e6 (Ce6) and modified with folic acid (FA) on the surface, can be used as versatile drug delivery vehicles for effective PDT. The nanoparticles are great carriers for photosensitizer Ce6 with an extremely high loading efficiency. In vitro fluorescence imaging and in vivo magnetic resonance imaging (MRI) results indicated that SCFs selectively accumulated in tumor cells. Under near-infrared laser irradiation, SCFs were confirmed to be capable of inducing low cell viability of RM-1 cells In vitro and displaying efficient tumor ablation with negligible side effects in tumor-bearing mice models.

Discovery of a Monoiodo Aza-BODIPY Near-Infrared Photosensitizer: in vitro and in vivo Evaluation for Photodynamic Therapy

2020 ◽  
Vol 63 (17) ◽  
pp. 9950-9964 ◽  
Author(s):  
Zhiliang Yu ◽  
Junliang Zhou ◽  
Xin Ji ◽  
Guangyu Lin ◽  
Shuang Xu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Near Infrared ◽  
In Vivo Evaluation

Highly Efficient Photosensitizers with Far-Red/Near-Infrared Aggregation-Induced Emission for In Vitro and In Vivo Cancer Theranostics

2018 ◽  
Vol 30 (39) ◽  
pp. 1802105 ◽  
Author(s):  
Dong Wang ◽  
Michelle M. S. Lee ◽  
Guogang Shan ◽  
Ryan T. K. Kwok ◽  
Jacky W. Y. Lam ◽  
...  
Keyword(s):  
Near Infrared ◽  
Aggregation Induced Emission ◽  
Highly Efficient ◽  
Cancer Theranostics

scholarly journals Precise and Efficient Phototheranostics: Molecular Engineering of Photosensitizers with Near-Infrared Aggregation-Induced Emission for Acid-Triggered Nucleus-Targeted Photodynamic Cancer Therapy

2020 ◽  
Author(s):  
Zhijun Zhang ◽  
Wenhan XU ◽  
Peihong Xiao ◽  
Miaomiao Kang ◽  
Dingyuan Yan ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Targeted Delivery ◽  
Near Infrared ◽  
Fluorescence Emission ◽  
Molecular Engineering ◽  
Ros Generation ◽  
Subcellular Targeting ◽  
Aggregation Induced Emission ◽  
Photodynamic Cancer Therapy

Phototheranostics involving both fluorescence imaging (FLI) and photodynamic therapy (PDT) has been recognized to be potentially powerful for cancer treatment by virtue of various intrinsic advantages. However, the state-of-the-art materials in this area are still far from ideal towards practical applications, owing to their respective and collective drawbacks, such as inefficient imaging quality, inferior reactive oxygen species (ROS) production, the lack of subcellular-targeting capability, and dissatisfactory theranostics delivery. In this contribution, these shortcomings are successfully addressed through the integration of finely engineered photosensitizers having aggregation-induced emission (AIE) features and well tailored nanocarrier system. The yielded AIE NPs simultaneously exhibit broad absorption in visible light region, bright near-infrared fluorescence emission, extremely high ROS generation, as well as tumor lysosomal acidity-activated and nucleus-targeted delivery functions, making them dramatically promising for precise and efficient phototheranostics. Both in vitro and in vivo evaluations show that the presented nanotheranostic system bearing excellent photostability and appreciable biosecurity well performed in FLI-guided photodynamic cancer therapy. This study thus not only extends the applications scope of AIE nanomaterials, but also offers useful insights into constructing a new generation of cancer theranostics.

scholarly journals Time-dependent Photodynamic Therapy for Multiple Targets: A Highly Efficient AIE-active Photosensitizer for Selective Bacterial Elimination and Cancer Cell Ablation

2019 ◽  
Author(s):  
Qiyao Li ◽  
Ying Li ◽  
Tianliang Min ◽  
Junyi Gong ◽  
Lili Du ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Reactive Oxygen Species Generation ◽  
Emission Feature ◽  
Time Dependent ◽  
Organic Salt ◽  
Antibacterial Performance ◽  
Cell Ablation ◽  
External Conditions

Pathogen infection and cancer are the two major human health problems. In this work, we achieved an organic salt photosensitizer (PS), called 4TPA-BQ with aggregation-induced emission feature <i>via</i> one-step reaction. Owing to the aggregation-induced reactive oxygen species generation effect and sufficient small ΔE<sub>ST</sub>, 4TPA-BQ shows a satisfactorily high <sup>1</sup>O<sub>2</sub> generation efficiency of 97.8%. <i>In vitro</i> and <i>in vivo</i> experiments confirmed that 4TPA-BQ exhibited potent photodynamic antibacterial performance against ampicillin-resistant <i>Escherichia coli</i> with good biocompatibility in a short time (15 min). When the incubation time persisted long enough (12 h), cancer cells were ablated efficiently, leaving normal cells essentially unaffected. This is the first reported time-dependent fluorescence-guided photodynamic therapy in one individual PS for ordered and multiple targeting by varying the external conditions. This can update the design principle of efficient PSs in potential clinical applications.

scholarly journals Embedded Upconversion Nanoparticles in Magnetic Molecularly Imprinted Polymers for Photodynamic Therapy of Hepatocellular Carcinoma

Biomedicines ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1923
Author(s):  
Cheng-Chih Lin ◽  
Hung-Yin Lin ◽  
James L. Thomas ◽  
Jia-Xin Yu ◽  
Chien-Yu Lin ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Molecularly Imprinted Polymers ◽  
Near Infrared ◽  
Malignant Tumors ◽  
Peptide Sequence ◽  
Upconversion Nanoparticles ◽  
Continuous Administration ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

Programmed death-ligand 1 protein (PD-L1) is often expressed in various malignant tumors; thus, it is an appropriate marker for targeted cancer therapies. Photodynamic therapy (PDT) uses light and photosensitizers to create singlet oxygen to kill cells. An important approach to PDT is the use of upconversion nanoparticles (UCNPs) to convert near-infrared (NIR) light, which penetrates tissues well, into visible light, allowing PDT to be effective at greater tissue depths. In this work, high-temperature pyrolysis was used to prepare both the core and shell of lanthanide-doped UCNPs with lithium yttrium tetrafluoride (LiYF4) to enhance the green luminescence. The photosensitizer Merocyanine 540 (MC540) was grafted onto the magnetic nanoparticles, and then one peptide sequence from PD-L1 was used as the template and imprinted onto poly(ethylene-co-vinyl alcohol) particles formed by precipitation in a non-solvent. UCNPs in the non-solvent bath were thus entrapped in the imprinted particles to generate composite nanoparticles for the targeting and photodynamic therapy of PD-L1 in tumor cells. Finally, the in vitro cytotoxicity of the nanoparticles in HepG2 human liver cancer cells was evaluated with the continuous administration of MC540/MNPs@MIPs/UCNPs under irradiation by an NIR laser. To understand the delivery of the UCNP-embedded molecularly imprinted polymers, the intrinsic and extrinsic pathways were also investigated.

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