Embedded Upconversion Nanoparticles in Magnetic Molecularly Imprinted Polymers for Photodynamic Therapy of Hepatocellular Carcinoma

Programmed death-ligand 1 protein (PD-L1) is often expressed in various malignant tumors; thus, it is an appropriate marker for targeted cancer therapies. Photodynamic therapy (PDT) uses light and photosensitizers to create singlet oxygen to kill cells. An important approach to PDT is the use of upconversion nanoparticles (UCNPs) to convert near-infrared (NIR) light, which penetrates tissues well, into visible light, allowing PDT to be effective at greater tissue depths. In this work, high-temperature pyrolysis was used to prepare both the core and shell of lanthanide-doped UCNPs with lithium yttrium tetrafluoride (LiYF4) to enhance the green luminescence. The photosensitizer Merocyanine 540 (MC540) was grafted onto the magnetic nanoparticles, and then one peptide sequence from PD-L1 was used as the template and imprinted onto poly(ethylene-co-vinyl alcohol) particles formed by precipitation in a non-solvent. UCNPs in the non-solvent bath were thus entrapped in the imprinted particles to generate composite nanoparticles for the targeting and photodynamic therapy of PD-L1 in tumor cells. Finally, the in vitro cytotoxicity of the nanoparticles in HepG2 human liver cancer cells was evaluated with the continuous administration of MC540/MNPs@MIPs/UCNPs under irradiation by an NIR laser. To understand the delivery of the UCNP-embedded molecularly imprinted polymers, the intrinsic and extrinsic pathways were also investigated.

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A multifunctional near-infrared fluorescent sensing material based on core-shell upconversion nanoparticles@magnetic nanoparticles and molecularly imprinted polymers for detection of deltamethrin

Microchimica Acta ◽

10.1007/s00604-021-04811-3 ◽

2021 ◽

Vol 188 (5) ◽

Author(s):

Ting Guo ◽

Changchang Wang ◽

Hongyuan Zhou ◽

Yuhao Zhang ◽

Liang Ma

Keyword(s):

Magnetic Nanoparticles ◽

Molecularly Imprinted Polymers ◽

Near Infrared ◽

Upconversion Nanoparticles ◽

Core Shell ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Fluorescent Sensing ◽

Sensing Material

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Simultaneous extraction of several targets by using non-toxic dual template molecularly imprinted polymers in vivo and in vitro

Talanta ◽

10.1016/j.talanta.2020.121283 ◽

2020 ◽

Vol 219 ◽

pp. 121283

Author(s):

Jia-Wei Zhang ◽

Jia-Yuan He ◽

Chong-Zhi Wang ◽

Feng-Qing Yang ◽

Lian-Di Zhou ◽

...

Keyword(s):

Molecularly Imprinted Polymers ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Simultaneous Extraction

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Development of Water-Compatible Molecularly Imprinted Polymers Based on Functionalized β-Cyclodextrin for Controlled Release of Atropine

Polymers ◽

10.3390/polym12010130 ◽

2020 ◽

Vol 12 (1) ◽

pp. 130 ◽

Cited By ~ 1

Author(s):

Yahui He ◽

Shaomei Zeng ◽

A. M. Abd El-Aty ◽

Ahmet Hacımüftüoğlu ◽

Woldemariam Kalekristos Yohannes ◽

...

Keyword(s):

Drug Delivery ◽

Controlled Release ◽

Molecularly Imprinted Polymers ◽

Isotherm Models ◽

Template Molecule ◽

Stimuli Responsive ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Potential Applications

Herein, a novel method for molecularly imprinted polymers (MIPs) using methacrylic acid functionalized beta-cyclodextrin (MAA-β-CD) monomer is presented, which was designed as a potential water-compatible composite for the controlled release of atropine (ATP). The molecularly imprinted microspheres with pH-sensitive characteristics were fabricated using thermally-initiated precipitation polymerization, employing ATP as a template molecule. The effects of different compounds and concentrations of cross-linking agents were systematically investigated. Uniform microspheres were obtained when the ratio between ATP, MAA-β-CD, and trimethylolpropane trimethacrylate (TRIM) was 1:4:20 (mol/mol/mol) in polymerization system. The ATP loading equilibrium data was best suited to the Freundlich and Langmuir isotherm models. The in vitro drug release study was assessed under simulated oral administration conditions (pH 1.5 and 7.4). The potential usefulness of MIPs as drug delivery devices are much better than non-molecularly imprinted polymers (NIPs). The study shows that the prepared polymers are a pH stimuli-responsive system, which controlled the release of ATP, indicating the potential applications in the field of drug delivery.

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300 Contractile response of bovine lateral saphenous vein to ergotamine tartrate exposed to molecularly imprinted polymers – Physiological significance of in vitro studies

Journal of Animal Science ◽

10.2527/asasann.2017.300 ◽

2017 ◽

Vol 95 (suppl_4) ◽

pp. 148-149

Author(s):

M. B. Kudupoje ◽

E. S. Vanzant ◽

A. Yiannikouris ◽

K. A. Dawson ◽

K. R. McLeod ◽

...

Keyword(s):

Saphenous Vein ◽

Molecularly Imprinted Polymers ◽

Contractile Response ◽

In Vitro Studies ◽

Physiological Significance ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Ergotamine Tartrate

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Molecularly Imprinted Polymers (MIPs) as Theranostic Systems for Sunitinib Controlled Release and Self-Monitoring in Cancer Therapy

Pharmaceutics ◽

10.3390/pharmaceutics12010041 ◽

2020 ◽

Vol 12 (1) ◽

pp. 41 ◽

Cited By ~ 9

Author(s):

Ortensia Ilaria Parisi ◽

Mariarosa Ruffo ◽

Rocco Malivindi ◽

Anna Francesca Vattimo ◽

Vincenzo Pezzi ◽

...

Keyword(s):

Controlled Release ◽

Cancer Therapy ◽

Molecularly Imprinted Polymers ◽

Rhodamine 6G ◽

In Vitro Release ◽

Self Monitoring ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Vitro Release

Cytotoxic agents that are used conventionally in cancer therapy present limitations that affect their efficacy and safety profile, leading to serious adverse effects. In the aim to overcome these drawbacks, different approaches have been investigated and, among them, theranostics is attracting interest. This new field of medicine combines diagnosis with targeted therapy; therefore, the aim of this study was the preparation and characterization of Molecularly Imprinted Polymers (MIPs) selective for the anticancer drug Sunitinib (SUT) for the development of a novel theranostic system that is able to integrate the drug controlled release ability of MIPs with Rhodamine 6G as a fluorescent marker. MIPs were synthesized by precipitation polymerization and then functionalized with Rhodamine 6G by radical grafting. The obtained polymeric particles were characterized in terms of particles size and distribution, ξ-potential and fluorescent, and hydrophilic properties. Moreover, adsorption isotherms and kinetics and in vitro release properties were also investigated. The obtained binding data confirmed the selective recognition properties of MIP, revealing that SUT adsorption better fitted the Langmuir model, while the adsorption process followed the pseudo-first order kinetic model. Finally, the in vitro release studies highlighted the SUT controlled release behavior of MIP, which was well fitted with the Ritger-Peppas kinetic model. Therefore, the synthesized fluorescent MIP represents a promising material for the development of a theranostic platform for Sunitinib controlled release and self-monitoring in cancer therapy.

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Near Infrared Light Activated Upconversion Nanoparticles (UCNP) based Photodynamic Therapy of Prostate Cancers: an in vitro Study

Photodiagnosis and Photodynamic Therapy ◽

10.1016/j.pdpdt.2021.102616 ◽

2021 ◽

pp. 102616

Author(s):

Burcu Güleryüz ◽

Uğur Ünal ◽

Murat Gülsoy

Keyword(s):

Photodynamic Therapy ◽

Near Infrared ◽

In Vitro Study ◽

Vitro Study ◽

Infrared Light ◽

Upconversion Nanoparticles ◽

Near Infrared Light ◽

Prostate Cancers

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Towards A New Approach for the Description of Cyclo–2,4-Dihydroxybenzoate, A Substance Which Effectively Mimics Zearalenone in Imprinted Polymers Designed for Analyzing Selected Mycotoxins in Urine

International Journal of Molecular Sciences ◽

10.3390/ijms20071588 ◽

2019 ◽

Vol 20 (7) ◽

pp. 1588

Author(s):

Renata Gadzała-Kopciuch ◽

Katarzyna Kwaśniewska ◽

Agnieszka Ludwiczak ◽

Piotr Skrzyniarz ◽

Rafał Jakubowski ◽

...

Keyword(s):

Molecularly Imprinted Polymers ◽

Physicochemical Characteristics ◽

Crystallographic Analysis ◽

Template Molecule ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Relative Standard ◽

First Time

A method of purifying cyclododecyl 2,4-dihydroxybenzoate as a potential replacement template molecule for preparation of molecularly-imprinted polymers for isolation of zearalenone in urine was developed. Full physicochemical characteristics of cyclododecyl 2,4-dihydroxybenzoate for the first time included crystallographic analysis and molecular modelling, which made possible the determination of the similarity between the cyclododecyl 2,4-dihydroxybenzoate and zearalenone molecules. The obtained molecularly-imprinted polymers show very high in vitro selectivity towards zearalenone due to specific interactions (e.g., hydrogen bonding, molecular recognition interaction). The achieved extraction recovery exceeds 94% at the tested concentration levels (20–500 ng·mL−1) with a relative standard deviation below 2%. Immunosorbents were found to have lower recoveries (below 92.5%) and RSD value between 2 and 4% for higher concentrations of the studied substance (400 ng·mL−1).

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Interaction of LiYF4:Yb3+/Er3+/Ho3+/Tm3+@LiYF4:Yb3+ upconversion nanoparticles, molecularly imprinted polymers, and templates

RSC Advances ◽

10.1039/d0ra05771a ◽

2020 ◽

Vol 10 (59) ◽

pp. 35600-35610

Author(s):

Hsiu-Wen Chien ◽

Min-Ting Tsai ◽

Chien-Hsin Yang ◽

Rong-Ho Lee ◽

Tzong-Liu Wang

Keyword(s):

Polymer Nanocomposites ◽

Molecular Imprinting ◽

Molecularly Imprinted Polymers ◽

Upconversion Nanoparticles ◽

Luminescent Materials ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Molecular Imprinting Polymer

In this work, LiYF4:Yb0.253+/Er0.013+/Tm0.013+/Ho0.013+@LiYF4:Yb0.23+ upconverting nanoparticles (UCNP) were used as luminescent materials for the preparation of molecular imprinting polymer nanocomposites.

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