Uptake, depuration and bioconcentration of bisphenol AF (BPAF) in whole-body and tissues of zebrafish (Danio rerio)

2016 ◽  
Vol 132 ◽  
pp. 339-344 ◽  
Author(s):  
Jiachen Shi ◽  
Yunjia Yang ◽  
Jing Zhang ◽  
Yixing Feng ◽  
Bing Shao
Keyword(s):  
Danio Rerio ◽  
Whole Body ◽  
Bisphenol Af

Whole body proteome response to a dietary lysine imbalance in zebrafish Danio rerio

2011 ◽  
Vol 6 (2) ◽  
pp. 178-186 ◽  
Author(s):  
Pedro Gómez-Requeni ◽  
Mahaut de Vareilles ◽  
Katerina Kousoulaki ◽  
Ann-Elise O. Jordal ◽  
Luis E.C. Conceição ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Whole Body

scholarly journals Effects of Metazachlor on Vitellogenin Induction in Zebrafish (Danio rerio)

2007 ◽  
Vol 76 (8) ◽  
pp. S61-S66 ◽  
Author(s):  
J. Jurčíková ◽  
P. Mikula ◽  
R. Dobšíková ◽  
D. Némethová ◽  
Z. Svobodová
Keyword(s):  
Danio Rerio ◽  
Sandwich Elisa ◽  
Whole Body ◽  
Control Fish ◽  
Ambient Water ◽  
Control Groups ◽  
Water Exposure ◽  
Vitellogenin Synthesis ◽  
Chloroacetanilide Herbicide

The influence of metazachlor on vitellogenesis in juvenile (20 days old) zebrafish (Danio rerio) was investigated after ambient water exposure to concentrations of 0.1, 1.0 and 5.0 mg l-1 of the chloroacetanilide herbicide Butisan 400 SC containing approximately 35.6% (w/w) metazachlor. After 20 days of exposure, vitellogenin concentrations in whole-body homogenates of the fish were measured by direct sandwich ELISA. The results were compared to vitellogenin concentrations in fish from both negative (no exposure) and positive (exposed to natural oestrogen 17β-oestradiol) control groups. Exposure to Butisan 400 SC at a concentration of 5.0 mg l-1 induced vitellogenin synthesis significantly compared to the control fish (p < 0.05). The oestrogenic effect of 17β-oestradiol was confirmed.

Use of a carbonic anhydrase Ca17a knockout to investigate mechanisms of ion uptake in zebrafish (Danio rerio)

2021 ◽  
Vol 320 (1) ◽  
pp. R55-R68
Author(s):  
Alex M. Zimmer ◽  
Milica Mandic ◽  
Hong Meng Yew ◽  
Emma Kunert ◽  
Yihang K. Pan ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Danio Rerio ◽  
Ammonia Excretion ◽  
Ion Uptake ◽  
Whole Body ◽  
Acid Base Balance ◽  
Acid Base ◽  
Uptake Mechanism ◽  
Uptake Rates ◽  
Base Balance

In fishes, branchial cytosolic carbonic anhydrase (CA) plays an important role in ion and acid-base regulation. The Ca17a isoform in zebrafish ( Danio rerio) is expressed abundantly in Na+-absorbing/H+-secreting H+-ATPase-rich (HR) cells. The present study aimed to identify the role of Ca17a in ion and acid-base regulation across life stages using CRISPR/Cas9 gene editing. However, in preliminary experiments, we established that ca17a knockout is lethal with ca17a−/− mutants exhibiting a significant decrease in survival beginning at ∼12 days postfertilization (dpf) and with no individuals surviving past 19 dpf. Based on these findings, we hypothesized that ca17a−/− mutants would display alterations in ion and acid-base balance and that these physiological disturbances might underlie their early demise. Na+ uptake rates were significantly increased by up to 300% in homozygous mutants compared with wild-type individuals at 4 and 9 dpf; however, whole body Na+ content remained constant. While Cl− uptake was significantly reduced in ca17a−/− mutants, Cl− content was unaffected. Reduction of CA activity by Ca17a morpholino knockdown or ethoxzolamide treatments similarly reduced Cl− uptake, implicating Ca17a in the mechanism of Cl− uptake by larval zebrafish. H+ secretion, O2 consumption, CO2 excretion, and ammonia excretion were generally unaltered in ca17a−/− mutants. In conclusion, while the loss of Ca17a caused marked changes in ion uptake rates, providing strong evidence for a Ca17a-dependent Cl− uptake mechanism, the underlying causes of the lethality of this mutation in zebrafish remain unclear.

scholarly journals Respirometry and cutaneous oxygen flux measurements reveal a negligible aerobic cost of ion regulation in larval zebrafish (Danio rerio)

2020 ◽  
Vol 223 (18) ◽  
pp. jeb226753
Author(s):  
Julian J. Parker ◽  
Alex M. Zimmer ◽  
Steve F. Perry
Keyword(s):  
Danio Rerio ◽  
Oxygen Flux ◽  
Whole Body ◽  
Uptake Capacity ◽  
Ion Regulation ◽  
Larval Zebrafish ◽  
Morpholino Knockdown ◽  
Rearing Conditions ◽  
Flux Measurements ◽  
Energy Consuming

ABSTRACTFishes living in fresh water counter the passive loss of salts by actively absorbing ions through specialized cells termed ionocytes. Ionocytes contain ATP-dependent transporters and are enriched with mitochondria; therefore ionic regulation is an energy-consuming process. The purpose of this study was to assess the aerobic costs of ion transport in larval zebrafish (Danio rerio). We hypothesized that changes in rates of Na+ uptake evoked by acidic or low Na+ rearing conditions would result in corresponding changes in whole-body oxygen consumption (ṀO2) and/or cutaneous oxygen flux (JO2), measured at the ionocyte-expressing yolk sac epithelium using the scanning micro-optrode technique (SMOT). Larvae at 4 days post-fertilization (dpf) that were reared under low pH (pH 4) conditions exhibited a higher rate of Na+ uptake compared with fish reared under control conditions (pH 7.6), yet they displayed a lower ṀO2 and no difference in cutaneous JO2. Despite a higher Na+ uptake capacity in larvae reared under low Na+ conditions, there were no differences in ṀO2 and JO2 at 4 dpf. Furthermore, although Na+ uptake was nearly abolished in 2 dpf larvae lacking ionocytes after morpholino knockdown of the ionocyte proliferation regulating transcription factor foxi3a, ṀO2 and JO2 were unaffected. Finally, laser ablation of ionocytes did not affect cutaneous JO2. Thus, we conclude that the aerobic costs of ion uptake by ionocytes in larval zebrafish, at least in the case of Na+, are below detection using whole-body respirometry or cutaneous SMOT scans, providing evidence that ion regulation in zebrafish larvae incurs a low aerobic cost.

scholarly journals Angiotensin-II promotes Na+ uptake in larval zebrafish, Danio rerio, in acidic and ion-poor water

2013 ◽  
Vol 220 (3) ◽  
pp. 195-205 ◽  
Author(s):  
Yusuke Kumai ◽  
Nicholas J Bernier ◽  
Steve F Perry
Keyword(s):  
Angiotensin Ii ◽  
Danio Rerio ◽  
Glucocorticoid Receptors ◽  
Enzyme Inhibitor ◽  
Renin Angiotensin System ◽  
Whole Body ◽  
Ang Ii ◽  
Larval Zebrafish ◽  
Rapid Activation ◽  
Stimulation Of

The contribution of the renin–angiotensin system (RAS) to Na+uptake was investigated in larval zebrafish (Danio rerio). At 4 days post fertilization (dpf), the level of whole-body angiotensin-II (ANG-II) was significantly increased after 1- or 3-h exposure to acidic (pH=4.0) or ion-poor water (20-fold dilution of Ottawa tapwater), suggesting rapid activation of the RAS. Long-term (24 h) treatment of 3 dpf larvae with ANG-I or ANG-II significantly increased Na+uptake which was accompanied by an increase in mRNA expression of the Na+-Cl−cotransporter (zslc12a10.2). Induction of Na+uptake by exposure to ANG-I was blocked by simultaneously treating larvae with lisinopril (an angiotensin-converting enzyme inhibitor). Acute (2 h) exposure to acidic water or ion-poor water led to significant increase in Na+uptake which was partially blocked by the ANG-II receptor antagonist, telmisartan. Consistent with these data, translational knockdown of renin prevented the stimulation of Na+uptake following exposure to acidic or ion-poor water. The lack of any effects of pharmacological inhibition (using RU486), or knockdown of glucocorticoid receptors on the stimulation of Na+uptake during acute exposure to acidic or ion-poor environments, indicates that the acute effects of RAS occur independently of cortisol signaling. The results of this study demonstrate that the RAS is involved in Na+homeostasis in larval zebrafish.

Evidence that SLC26 anion transporters mediate branchial chloride uptake in adult zebrafish (Danio rerio)

2009 ◽  
Vol 297 (4) ◽  
pp. R988-R997 ◽  
Author(s):  
S. F. Perry ◽  
B. Vulesevic ◽  
M. Grosell ◽  
M. Bayaa
Keyword(s):  
Mrna Expression ◽  
Danio Rerio ◽  
Whole Body ◽  
Mrna Levels ◽  
Adult Zebrafish ◽  
Chloride Uptake ◽  
Anion Transporters ◽  
Anion Transporter ◽  
Normal Water

Experiments were performed to test the hypothesis that three members of the SLC26 anion transporter gene family (SLC26a3, A4, and A6; hereafter termed za3, za4, and za6) mediate branchial Cl−/HCO3− exchange in adult zebrafish ( Danio rerio). Real-time RT-PCR demonstrated that the gill expressed relatively high levels of za6 mRNA; za3 and za4 mRNA, while present, were less abundant. Also, za4 and za6 were expressed at relatively high levels in the kidney. The results of in situ hybridization or immunocytochemistry (za3 only) experiments performed on gill sections revealed that the SLC26 transporters were predominantly expressed on the filament epithelium (especially within the interlamellar regions) and to a lesser extent on the lamellar epithelium at the base of lamellae. This distribution pattern suggests that the SLC26 anion transporters are localized to mitochondrion-rich cells (ionocytes). Transferring fish to water containing low [Cl−] (0.02 mmol/l) resulted in significant increases in branchial SLC26 mRNA expression after 5–10 days of exposure relative to fish raised in normal water [Cl−] (0.4 mmol/l); transferring fish to Cl−-enriched water (2.0 mmol/l) was without effect on mRNA levels. Transferring fish to water containing elevated levels of NaHCO3 (10–12.5 mmol/l) caused marked increases in branchial SLC26 mRNA expression between 3 and 10 days of transfer that was associated with a significant 40% increase in Cl− uptake (as measured upon return to normal water after 7 days). A decrease in whole body net acid excretion (equivalent to an increase in net base excretion) in fish previously maintained in high [NaHCO3] water, concurrent with increases in Cl− uptake and SLC26 mRNA levels, suggests a role for these anion transporters in Cl− uptake and acid-base regulation owing to their Cl−/HCO3− exchange activities.

Whole-body cortisol increases after direct and visual contact with a predator in zebrafish, Danio rerio

Aquaculture ◽  
2007 ◽  
Vol 272 (1-4) ◽  
pp. 774-778 ◽  
Author(s):  
Leonardo José Gil Barcellos ◽  
Filipe Ritter ◽  
Luiz Carlos Kreutz ◽  
Rosmari Mezzalira Quevedo ◽  
Leonardo Bolognesi da Silva ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Whole Body ◽  
Visual Contact

scholarly journals Expression of ion transport genes in ionocytes isolated from larval zebrafish (Danio rerio) exposed to acidic or Na+-deficient water

2020 ◽  
Vol 319 (4) ◽  
pp. R412-R427
Author(s):  
K. Shir-Mohammadi ◽  
S. F. Perry
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Ion Transport ◽  
Danio Rerio ◽  
Ionic Composition ◽  
Low Ph ◽  
Activity Level ◽  
Whole Body ◽  
Mrna Levels ◽  
Acid Base

In zebrafish ( Danio rerio), a specific ionocyte subtype, the H+-ATPase-rich (HR) cell, is presumed to be a significant site of transepithelial Na+ uptake/acid secretion. During acclimation to environments differing in ionic composition or pH, ionic and acid–base regulations are achieved by adjustments to the activity level of HR cell ion transport proteins. In previous studies, the quantitative assessment of mRNA levels for genes involved in ionic and acid–base regulations relied on measurements using homogenates derived from the whole body (larvae) or the gill (adult). Such studies cannot distinguish whether any differences in gene expression arise from adjustments of ionocyte subtype numbers or transcriptional regulation specifically within individual ionocytes. The goal of the present study was to use fluorescence-activated cell sorting to separate the HR cells from other cellular subpopulations to facilitate the measurement of gene expression of HR cell-specific transporters and enzymes from larvae exposed to low pH (pH 4.0) or low Na+ (5 μM) conditions. The data demonstrate that treatment of larvae with acidic water for 4 days postfertilization caused cell-specific increases in H+-ATPase ( atp6v1aa), ca17a, ca15a, nhe3b, and rhcgb mRNA in addition to increases in mRNA linked to cell proliferation. In fish exposed to low Na+, expression of nhe3b and rhcgb was increased owing to HR cell-specific regulation and elevated numbers of HR cells. Thus, the results of this study demonstrate that acclimation to low pH or low Na+ environmental conditions is facilitated by HR cell-specific transcriptional control and by HR cell proliferation.

Sign in / Sign up

Export Citation Format

Share Document