Autophagy and apoptosis mediated nano-copper-induced testicular damage

Objective: Investigating the effect of green tea extract (GTE) on the testicular damage induced by cadmium chloride CdCl2 in male rats. Design: Randomized controlled study. Animals: 40 male Wistar rats. Procedures: Rats were randomly divided into four groups: A) control group (each rat daily received pellet diet); B) GTE group each rat daily received pellet diet as well as 3 ml of 1.5 % w/v GTE, C) CdCl2 group each rat was I/P injected a single dose of 1 mg/kg CdCl2, then daily received pellet diet, and D) CdCl2+GTE group each rat was I/P injected a single dose of 1 mg/kg CdCl2 then daily received pellet diet as well as 3 ml of 1.5 % w/v GTE. After 30 days, blood samples were collected for hormonal assays (testosterone, FSH, and LH). In addition, both testes were collected; one of them was used for quantification of 17-beta hydroxysteroid dehydrogenase III (17β-HSDIII) gene expression using a real-time PCR. The other testis was used for determination of catalase and reduced glutathione; GSH, Nitric oxide (NO) and malondialdehyde (MDA) levels. Results: CdCl2 decreased serum testosterone levels and its synthesis pathway (17β-HSDIII testicular gene expression). While antioxidants catalase and GSH were reduced, oxidants MDA were enriched in the testes of CdCl2-poisoned rats. This CdCl2-promoted testicular dysfunction was corrected via the administration of GTE to male rats. Conclusion and clinical relevance: GTE could be used as a remedy for protecting against CdCl2-induced testicular damage in male rats.

Download Full-text

An SOD1 deficiency aggravates proteasome inhibitor bortezomib-induced testicular damage in mice

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/j.bbagen.2019.04.005 ◽

2019 ◽

Vol 1863 (6) ◽

pp. 1108-1115 ◽

Cited By ~ 4

Author(s):

Takujiro Homma ◽

Junichi Fujii

Keyword(s):

Proteasome Inhibitor ◽

Testicular Damage

Download Full-text

Trans-ferulic acid ameliorates cisplatin-induced testicular damage via suppression of TLR4, P38-MAPK, and ERK1/2 signaling pathways

Environmental Science and Pollution Research ◽

10.1007/s11356-021-13544-y ◽

2021 ◽

Author(s):

Emad H. M. Hassanein ◽

Basel A. Abdel-Wahab ◽

Fares E. M. Ali ◽

Omnia A. M. Abd El-Ghafar ◽

Magy R. Kozman ◽

...

Keyword(s):

Ferulic Acid ◽

Signaling Pathways ◽

P38 Mapk ◽

Testicular Damage

Download Full-text

Effects of resveratrol against scattered radiation-induced testicular damage in rats

Turkish Journal of Biochemistry ◽

10.1515/tjb-2020-0320 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Tarik Emre Sener ◽

Beste Melek Atasoy ◽

Ozge Cevik ◽

Ozlem Tugce Cilingir Kaya ◽

Sule Cetinel ◽

...

Keyword(s):

Ionizing Radiation ◽

Radiation Exposure ◽

Scattered Radiation ◽

Caspase 3 ◽

Sirtuin 1 ◽

Protective Agent ◽

Testicular Damage ◽

Testosterone Levels ◽

Protein Expressions ◽

Ionizing Radiation Exposure

AbstractObjectivesTo investigate the possible protective effects of resveratrol against oxidative testicular damage due to scattered radiation during pelvic ionizing radiation exposure in rats.MethodsRats were divided into 5 groups; control, radiation, and radiation + resveratrol therapy in early and late periods. Under anesthesia, 20 Gy ionizing radiation was applied to prostatic region. Resveratrol was administered (10 mg/kg/day) orally before ionizing radiation exposure. Animals were decapitated at the end of 1st and 10th weeks. Biochemical markers of oxidative stress; caspase-3 and sirtuin-1 protein expressions; testosterone levels were evaluated, histological examinations were performed.ResultsSignificant increases in malondialdehyde, 8-hydroxy-deoxyguanosine levels, myeloperoxidase, and caspase-3 activities were observed after ionizing radiation exposure, also superoxide dismutase and glutathione activities were significantly decreased. Radiotherapy increased caspase-3 and decreased sirtuin-1 protein expressions. Resveratrol treatment significantly reversed these parameters and also reversed the decrease in testosterone levels back to control levels in late period.ConclusionResveratrol showed antioxidant and sirtuin-activating properties against oxidative damage caused by scattered radiation to testis and provided hormonal protection. These results suggest that resveratrol may be an alternative protective agent on testicular tissues against the effects of scattered pelvic radiation.

Download Full-text

Male gonadal function after chemotherapy for solid tumors in childhood.

Journal of Clinical Oncology ◽

10.1200/jco.1989.7.3.304 ◽

1989 ◽

Vol 7 (3) ◽

pp. 304-309 ◽

Cited By ~ 104

Author(s):

F Aubier ◽

F Flamant ◽

R Brauner ◽

J M Caillaud ◽

J M Chaussain ◽

...

Keyword(s):

Toxic Effect ◽

Alkylating Agents ◽

Follicle Stimulating Hormone ◽

Normal Growth ◽

Testicular Biopsy ◽

Testicular Function ◽

Adult Males ◽

Gonadal Function ◽

Testicular Damage ◽

Rule Out

The testicular function of 30 adolescent or adult males having undergone polychemotherapy in childhood was assessed by means of a spermogram or testicular biopsy. At the time of examination, the patients were pubertal and had completed chemotherapy between 1 and 20 years previously (mean, 9 years). All patients who were prepubertal or intrapubertal at the time of treatment achieved normal puberty with normal growth. Twenty patients presented with azoospermia and/or severe disturbances in the germinal line on biopsy. This series confirms the toxicity of alkylating agents, in particular that of the mechlorethamine, vincristine, procarbazine, and prednisone combination (MOPP) and that of cyclophosphamide (CPM). However, dactinomycin, vinblastine, and vincristine did not appear to have a toxic effect on spermatogenesis. The prepubertal state did not protect the gonads of 19 patients who were prepubertal at diagnosis: 12 are now sterile as a result of the treatment. An increase in basal follicle-stimulating hormone (FSH) levels gives a good indication of testicular damage, although normal levels do not rule out the possibility of azoospermia.

Download Full-text