scholarly journals Techno-economic analysis of lipase enzyme production from Aspergillus niger using agro-industrial waste by solid state fermentation

2018 ◽  
Vol 153 ◽  
pp. 143-148 ◽  
Author(s):  
Andy Khootama ◽  
Dwini Normayulisa Putri ◽  
Heri Hermansyah
Author(s):  
MARIA ALICE ZARUR COELHO ◽  
SELMA GOMES FERREIRA LEITE ◽  
MORSYLEIDE DE FREITAS ROSA ◽  
ANGELA APARECIDA LEMOS FURTADO

Investigou-se o aproveitamento da casca do coco verde, mediante fermentação semisólida, para produção de enzimas. A casca de coco foi previamente desidratada, moída e classificada em três diferentes granulometrias, ou seja, 14, 28 e 32 mesh Tyler. Todas as enzimas obtidas tiveram sua produção máxima na faixa de 24 e 96 horas, o que corresponde ao tempo de produção industrial corrente. Cada granulometria produziu complexos enzimáticos ricos em diferentes atividades. O estudo realizado validou a hipótese do aproveitamento do resíduo da casca do coco verde na produção de enzimas por Aspergillus niger. Abstract The utilization of immature coconut peel as substrate for enzyme production by solid state fermentation was investigated. The coconut peel was previously dehydrated, milled and classified in three distinct granulometries: 14, 28 and 32 mesh Tyler. All the enzymes obtained had its maximum production in 24 to 96 hour interval, which correspond to the current industrial production time. Each granulometry produced rich enzymatic complexes with different activities. This study validates the hypothesis of benefit immature coconut peel as raw material for enzyme production by Aspergillus niger.


2014 ◽  
Vol 2 (4) ◽  
pp. 488-492 ◽  
Author(s):  
OM Fasalu Rahiman ◽  
Musambil Mohthash ◽  
U Salmanul Faris ◽  
TK Mohammed Muneersha ◽  
M Shejina

Biotechnology techniques can provide an unlimited and pure source of enzymes as an alternative to the harsh chemicals traditionally used in industry for accelerating chemical reactions. L-asparaginase is one among them, found in various plants, animals and bacterial cells. Lasparaginase is studied to be responsible for catalyzing the deamination of Asparagines to yield Aspartic acid and an ammonium ion, resulting in depletion of free circulatory Asparagines in plasma. Its use in therapeutics is found to be remarkable, especially for those specific cases where blood cells become cancerous, such as in acute lymphoblastic leukemia. In this study we have made an effort to isolate, identify and screen micro-organism (Aspergillus niger) for the production of anticancerous enzyme (L-asparaginases) by solid state fermentation process and the produced enzyme was purified and characterized for L-asparaginases. The aim of the study was to validate the solid fermentation process in terms of its reliability and feasibility for production of L-asparaginase enzyme. This method was found to be very cost effective and reliable when compared to the other expensive techniques used around the globe for enzyme production. Even though the product yield and purity is comparatively less in comparison with the other techniques it can be still used as a reliable technique for short scale enzyme production. DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11278  Int J Appl Sci Biotechnol, Vol. 2(4): 488-492 


2020 ◽  
Vol 6 ◽  
pp. 331-335 ◽  
Author(s):  
Dwini Normayulisa Putri ◽  
Andy Khootama ◽  
Meka Saima Perdani ◽  
Tania Surya Utami ◽  
Heri Hermansyah

2017 ◽  
Vol 7 (5) ◽  
pp. 17
Author(s):  
Mirza M.V. Baig ◽  
Aniruddha Ratnakar Apastambh

The production of Pectic enzymes by Aspergillus niger was studied under solid state fermentation (SSF). The effect of fermentation condition such as substrate concentration, inoculum volume, incubation time, moistening agent, inducers and organic and inorganic nitrogen sources was studied for enzyme production. Culture conditions were optimized for maximal yield of enzyme. The solid substrate wheat bran was most suitable for pectic enzyme production under SSF. Enzyme production was found maximum after 10 days of incubation. Lactose was found to be most effective as inducer. Gelatin as organic nitrogen source and ammonium nitrate as inorganic nitrogen source yielded high enzyme titres.


2000 ◽  
Vol 36 (3) ◽  
pp. 255-261 ◽  
Author(s):  
Sonia Couri ◽  
Selma da Costa Terzi ◽  
Gustavo A Saavedra Pinto ◽  
Suely Pereira Freitas ◽  
Antonio Carlos Augusto da Costa

2019 ◽  
Vol 42 ◽  
pp. e41358
Author(s):  
Fernanda de Oliveira ◽  
Marcelo Rodrigues de Melo ◽  
João Batista Buzato

The low-cost production of cellulolytic complexes that present high action at mild conditions is one of the major bottlenecks for the economic viability of the production of cellulosic ethanol. The influence of agro-industrial residues was assessed to enhance endoglucanase production by Aspergillus niger 426 grown in solid state fermentation. The highest percentage of lignin degradation was found on soybean hulls (56%) followed by sugarcane bagasse (36%) and rice straw (8.5%). The cellulose degradation, around 90%, was observed on soybean hulls and sugarcane bagasse, but only 50% on rice straw, and maximum production of endoglucanase (112.34 ± 0.984 U mL-1) was observed for soybean hulls. The best Experimental Mixture Design condition was under cultivation of 2.5 g of sugarcane bagasse, 2.3 g of rice straw and 5.2 g of soybean hulls, leading to a maximum activity of 138.92 ± 0.02 U mL-1. The statistical methodology enabled an increase of over 20% in the production of endoglucanase using agro-industrial waste. These data demonstrate that A. niger 426 is a potential source of cellulases which can be obtained by solid state fermentation using agro-industrial waste.


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