Hydrolytic Enzyme Production by Aspergillus niger in Solid State Fermentation: Stimulation of Transcriptional Regulation Through Carbon and Nitrogen Supplementation

2010 ◽  
Vol 9 (1) ◽  
pp. 38-49
Author(s):  
Shauna McKelvey
Keyword(s):  
Transcriptional Regulation ◽  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Hydrolytic Enzyme ◽  
Nitrogen Supplementation ◽  
Carbon And Nitrogen ◽  
Stimulation Of

Hydrolytic enzyme production in solid-state fermentation by Aspergillus niger 3T5B8

2000 ◽  
Vol 36 (3) ◽  
pp. 255-261 ◽  
Author(s):  
Sonia Couri ◽  
Selma da Costa Terzi ◽  
Gustavo A Saavedra Pinto ◽  
Suely Pereira Freitas ◽  
Antonio Carlos Augusto da Costa
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Hydrolytic Enzyme

scholarly journals APROVEITAMENTO DE RESÍDUOS AGROINDUSTRIAIS: PRODUÇÃO DE ENZIMAS A PARTIR DA CASCA DE COCO VERDE

2001 ◽  
Vol 19 (1) ◽  
Author(s):  
MARIA ALICE ZARUR COELHO ◽  
SELMA GOMES FERREIRA LEITE ◽  
MORSYLEIDE DE FREITAS ROSA ◽  
ANGELA APARECIDA LEMOS FURTADO
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Industrial Production ◽  
Enzyme Production ◽  
Raw Material ◽  
Production Time ◽  
Maximum Production ◽  
E 32

Investigou-se o aproveitamento da casca do coco verde, mediante fermentação semisólida, para produção de enzimas. A casca de coco foi previamente desidratada, moída e classificada em três diferentes granulometrias, ou seja, 14, 28 e 32 mesh Tyler. Todas as enzimas obtidas tiveram sua produção máxima na faixa de 24 e 96 horas, o que corresponde ao tempo de produção industrial corrente. Cada granulometria produziu complexos enzimáticos ricos em diferentes atividades. O estudo realizado validou a hipótese do aproveitamento do resíduo da casca do coco verde na produção de enzimas por Aspergillus niger. Abstract The utilization of immature coconut peel as substrate for enzyme production by solid state fermentation was investigated. The coconut peel was previously dehydrated, milled and classified in three distinct granulometries: 14, 28 and 32 mesh Tyler. All the enzymes obtained had its maximum production in 24 to 96 hour interval, which correspond to the current industrial production time. Each granulometry produced rich enzymatic complexes with different activities. This study validates the hypothesis of benefit immature coconut peel as raw material for enzyme production by Aspergillus niger.

scholarly journals Evaluation of agricultural byproducts for the production of betaglucosidase by Aspergillus niger MBT-2 using solid state fermentation

2020 ◽  
Vol 49 (1) ◽  
pp. 135-140
Author(s):  
Roheena Abdullah ◽  
Maria Hanif ◽  
Afshan Kaleem ◽  
Mehwish Iqtedar ◽  
Kinza Nisar ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Low Cost ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Enhanced Production ◽  
Enzyme Productivity ◽  
Valuable Product ◽  
By Products

The experiment was conducted to isolate and screen fungal strain and optimization of solid-state fermentation conditions for enhanced production of β-glucosidase. Different fungal cultures were isolated and screened for β-glucosidase production. The physicochemical and nutritional parameters were optimized for enhanced production of β-glucosidase from higher producer. Among all the isolates the isolate which exhibited highest β-glucosidase potential was identified and assigned the code as Aspergillus niger MBT-2. The optimum β-glucosidase production was obtained in M5 medium containing wheat bran after 72 hrs of incubation at 40°C, pH 6 and 20 ml of moisture contents. In addition to this 2% fructose and 2% yeast extract proved to be best carbon and nitrogen sources, respectively and gave maximal enzyme productivity. The exploitation of agricultural by products as a substrate reduced the production cost of enzyme and makes the process economical. The Aspergillus niger MBT-2 has promising potential of bioconversion of low-cost material into valuable product like β-glucosidase.

scholarly journals Solid-state fermentation on poplar sawdust and corncob wastes for lignocellulolytic enzymes by different Pleurotus ostreatus strains

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 4982-4995
Author(s):  
Mei-Ling Han ◽  
Qi An ◽  
Sai-Fei He ◽  
Xiao-Lin Zhang ◽  
Ming-Hui Zhang ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Pleurotus Ostreatus ◽  
Enzyme Production ◽  
Hydrolytic Enzyme ◽  
Wild Strain ◽  
Xylanase Activity ◽  
Lignocellulosic Materials ◽  
Lignocellulolytic Enzymes ◽  
Poplar Sawdust

Solid state fermentation with different lignocellulolytic materials as inducers was used for lignocellulolytic enzyme production in this study. Pleurotus ostreatus strains were assessed by measuring laccase, CMCase, and xylanase activities. The secretion potential of the lignocellulolytic enzymes by wild and cultivated strains was analyzed for the first time. The wild and cultivated strain showed their unique capacities for secreting lignocellulolytic enzymes on solid-state fermentation with different lignocellulosic materials. The wild P. ostreatus strain preferred corncob for the secretion of laccase and xylanase activity, but the cultivated strain preferred poplar sawdust. The wild strain and cultivated strain showed a consistent preference for poplar sawdust for the secretion of CMCase activity. The wild strain was advantageous because it achieved the maximum hydrolytic enzyme activities within a short time period. Poplar sawdust and corncob were conducive to laccase secretion by the wild or cultivated strains and the rapid accumulation of laccase on solid-state fermentation. Additionally, continuous, stable laccase production was an extremely important advantage by solid-state fermentation of poplar sawdust, particularly in the wild strain. These findings are helpful in selecting the appropriate strain that corresponds to suitable lignocellulosic materials. The optimization of integrated industrial lignocellulolytic enzyme production can also be achieved.

scholarly journals Production, Purification and Standardization of Anticancerous Enzyme (L-Asparaginases) from Aspergillus Niger Using Soil Samples by Solid State Fermentation

2014 ◽  
Vol 2 (4) ◽  
pp. 488-492 ◽  
Author(s):  
OM Fasalu Rahiman ◽  
Musambil Mohthash ◽  
U Salmanul Faris ◽  
TK Mohammed Muneersha ◽  
M Shejina
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Fermentation Process ◽  
Lymphoblastic Leukemia ◽  
The Other ◽  
Ammonium Ion ◽  
Bacterial Cells ◽  
Reliable Technique

Biotechnology techniques can provide an unlimited and pure source of enzymes as an alternative to the harsh chemicals traditionally used in industry for accelerating chemical reactions. L-asparaginase is one among them, found in various plants, animals and bacterial cells. Lasparaginase is studied to be responsible for catalyzing the deamination of Asparagines to yield Aspartic acid and an ammonium ion, resulting in depletion of free circulatory Asparagines in plasma. Its use in therapeutics is found to be remarkable, especially for those specific cases where blood cells become cancerous, such as in acute lymphoblastic leukemia. In this study we have made an effort to isolate, identify and screen micro-organism (Aspergillus niger) for the production of anticancerous enzyme (L-asparaginases) by solid state fermentation process and the produced enzyme was purified and characterized for L-asparaginases. The aim of the study was to validate the solid fermentation process in terms of its reliability and feasibility for production of L-asparaginase enzyme. This method was found to be very cost effective and reliable when compared to the other expensive techniques used around the globe for enzyme production. Even though the product yield and purity is comparatively less in comparison with the other techniques it can be still used as a reliable technique for short scale enzyme production. DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11278  Int J Appl Sci Biotechnol, Vol. 2(4): 488-492 

Pectinase production from Aspergillus niger IBT-7 using solid state fermentation

2018 ◽  
Vol 47 (3) ◽  
pp. 473-478 ◽  
Author(s):  
Roheena Abdullah ◽  
Iqra Farooq ◽  
Afshan Kaleem ◽  
Mehwish Iqtedar ◽  
Tehreema Iftikhar
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Fruits And Vegetables ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Local Soil ◽  
Microscopic Studies ◽  
Pectinase Production

Different fungal strains were isolated from the local soil, fruits and vegetables on the basis of pectin hydrolysis. All the isolated strains were identified through microscopic studies and screened for pectinase production using solid state fermentation. The fungal strain identified as Aspergillus niger IBT-7 showed the highest pectinase production. The selected strain was further subjected to optimization through different physical and nutritional parameters to enhance the production of pectinase. Amongst seven different media tested M1 containing rice bran, moistened with Czapek’s nutrient medium showed the highest pectinase production. During optimization maximum pectinase production was achieved after 72 hrs of incubation at 30 ml of moisture content, pH 5.0 and 30°C. Xylose (1.5%) and yeast extract (1%) proved to be best supplemented carbon and nitrogen sources, respectively which gave the highest pectinase production (39.1 U/ml/min).

scholarly journals Solid-state fermentation for production of Pectic enzymes by Aspergillus niger

2017 ◽  
Vol 7 (5) ◽  
pp. 17
Author(s):  
Mirza M.V. Baig ◽  
Aniruddha Ratnakar Apastambh
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Nitrogen Source ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Solid Substrate ◽  
Inorganic Nitrogen Source ◽  
Pectic Enzymes

The production of Pectic enzymes by Aspergillus niger was studied under solid state fermentation (SSF). The effect of fermentation condition such as substrate concentration, inoculum volume, incubation time, moistening agent, inducers and organic and inorganic nitrogen sources was studied for enzyme production. Culture conditions were optimized for maximal yield of enzyme. The solid substrate wheat bran was most suitable for pectic enzyme production under SSF. Enzyme production was found maximum after 10 days of incubation. Lactose was found to be most effective as inducer. Gelatin as organic nitrogen source and ammonium nitrate as inorganic nitrogen source yielded high enzyme titres.

scholarly journals Saccharification and hydrolytic enzyme production of alkali pre-treated wheat bran by Trichoderma virens under solid state fermentation

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Reda M. El-Shishtawy ◽  
Saleh A. Mohamed ◽  
Abdullah M. Asiri ◽  
Abu-bakr M. Gomaa ◽  
Ibrahim H. Ibrahim ◽  
...  
Keyword(s):  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Hydrolytic Enzyme ◽  
Trichoderma Virens

scholarly journals Characterization of β-Glucosidase Produced by Aspergillus niger under Solid-State Fermentation and Partially Purified Using MANAE-Agarose

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Anderson Baraldo Junior ◽  
Diogo G. Borges ◽  
Paulo W. Tardioli ◽  
Cristiane S. Farinas
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Specific Activity ◽  
Hydrolytic Enzyme ◽  
Single Step ◽  
Size Exclusion ◽  
Biotechnological Applications ◽  
Chromatography Analysis ◽  
Exclusion Chromatography

β-Glucosidase (BGL) is a hydrolytic enzyme with specificity for a wide variety of glycoside substrates, being an enzyme with a large range of biotechnological applications. However, enzyme properties can be different depending both on the microorganism and the cultivation procedure employed. Therefore, in order to explore potential biocatalytical applications of novel enzymes, their characterization is essential. In this work, a BGL synthesized by a selected strain of Aspergillus niger cultivated under solid-state fermentation (SSF) was partially purified and fully characterized in terms of optimum pH, temperature, and thermostability. The single-step purification using MANAE-agarose in a chromatographic column yielded an enzyme solution with specific activity (17.1 IU/mg protein) adequate for the characterization procedures. Electrophoresis SDS-PAGE and size-exclusion chromatography analysis resulted in an estimated molecular mass of 60 kDa. Higher enzyme activities were found in the range between 40 and 65°C and between pH 4 and 5.5, indicating an interesting characteristic for application in the hydrolysis of lignocellulosic biomass for biofuels production. Thermostability studies of purified BGL resulted in half-lives at 37°C of 56.3 h and at 50°C of 5.4 h. These results provide support for further studies of this enzyme towards revealing its potential biotechnological applications.

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