scholarly journals Production, Purification and Standardization of Anticancerous Enzyme (L-Asparaginases) from Aspergillus Niger Using Soil Samples by Solid State Fermentation

2014 ◽  
Vol 2 (4) ◽  
pp. 488-492 ◽  
Author(s):  
OM Fasalu Rahiman ◽  
Musambil Mohthash ◽  
U Salmanul Faris ◽  
TK Mohammed Muneersha ◽  
M Shejina
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Fermentation Process ◽  
Lymphoblastic Leukemia ◽  
The Other ◽  
Ammonium Ion ◽  
Bacterial Cells ◽  
Reliable Technique

Biotechnology techniques can provide an unlimited and pure source of enzymes as an alternative to the harsh chemicals traditionally used in industry for accelerating chemical reactions. L-asparaginase is one among them, found in various plants, animals and bacterial cells. Lasparaginase is studied to be responsible for catalyzing the deamination of Asparagines to yield Aspartic acid and an ammonium ion, resulting in depletion of free circulatory Asparagines in plasma. Its use in therapeutics is found to be remarkable, especially for those specific cases where blood cells become cancerous, such as in acute lymphoblastic leukemia. In this study we have made an effort to isolate, identify and screen micro-organism (Aspergillus niger) for the production of anticancerous enzyme (L-asparaginases) by solid state fermentation process and the produced enzyme was purified and characterized for L-asparaginases. The aim of the study was to validate the solid fermentation process in terms of its reliability and feasibility for production of L-asparaginase enzyme. This method was found to be very cost effective and reliable when compared to the other expensive techniques used around the globe for enzyme production. Even though the product yield and purity is comparatively less in comparison with the other techniques it can be still used as a reliable technique for short scale enzyme production. DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11278  Int J Appl Sci Biotechnol, Vol. 2(4): 488-492 

scholarly journals APROVEITAMENTO DE RESÍDUOS AGROINDUSTRIAIS: PRODUÇÃO DE ENZIMAS A PARTIR DA CASCA DE COCO VERDE

2001 ◽  
Vol 19 (1) ◽  
Author(s):  
MARIA ALICE ZARUR COELHO ◽  
SELMA GOMES FERREIRA LEITE ◽  
MORSYLEIDE DE FREITAS ROSA ◽  
ANGELA APARECIDA LEMOS FURTADO
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Industrial Production ◽  
Enzyme Production ◽  
Raw Material ◽  
Production Time ◽  
Maximum Production ◽  
E 32

Investigou-se o aproveitamento da casca do coco verde, mediante fermentação semisólida, para produção de enzimas. A casca de coco foi previamente desidratada, moída e classificada em três diferentes granulometrias, ou seja, 14, 28 e 32 mesh Tyler. Todas as enzimas obtidas tiveram sua produção máxima na faixa de 24 e 96 horas, o que corresponde ao tempo de produção industrial corrente. Cada granulometria produziu complexos enzimáticos ricos em diferentes atividades. O estudo realizado validou a hipótese do aproveitamento do resíduo da casca do coco verde na produção de enzimas por Aspergillus niger. Abstract The utilization of immature coconut peel as substrate for enzyme production by solid state fermentation was investigated. The coconut peel was previously dehydrated, milled and classified in three distinct granulometries: 14, 28 and 32 mesh Tyler. All the enzymes obtained had its maximum production in 24 to 96 hour interval, which correspond to the current industrial production time. Each granulometry produced rich enzymatic complexes with different activities. This study validates the hypothesis of benefit immature coconut peel as raw material for enzyme production by Aspergillus niger.

scholarly journals A comparison of methods to determine tannin acyl hydrolase activity

1999 ◽  
Vol 42 (3) ◽  
pp. 355-362 ◽  
Author(s):  
Cristóbal Aguilar ◽  
Augur Christopher ◽  
Gustavo Viniegra-González ◽  
Ernesto Favela
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Polyurethane Foam ◽  
Solid State Fermentation ◽  
The Other ◽  
Comparison Of Methods ◽  
Hplc Assay ◽  
Spectrophotometric Methods ◽  
Ph Values ◽  
Hplc Technique

Six methods to determine the activity of tannase produced by Aspergillus niger Aa-20 on polyurethane foam by solid state fermentation, which included two titrimetric techniques, three spectrophotometric methods and one HPLC assay were tested and compared. All methods assayed enabled the measurement of extracellular tannase activity. However, only five were useful to evaluate intracellular tannase activity. Studies on the effect of pH on tannase extraction demonstrated that tannase activity was considerably under-estimated when its extraction was carried out at pH values below 5.5 and above 6.0. Results showed that the HPLC technique and the modified Bajpai and Patil methods presented several advantages in comparison to the other methods tested.

scholarly journals Solid-state fermentation for production of Pectic enzymes by Aspergillus niger

2017 ◽  
Vol 7 (5) ◽  
pp. 17
Author(s):  
Mirza M.V. Baig ◽  
Aniruddha Ratnakar Apastambh
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Nitrogen Source ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Solid Substrate ◽  
Inorganic Nitrogen Source ◽  
Pectic Enzymes

The production of Pectic enzymes by Aspergillus niger was studied under solid state fermentation (SSF). The effect of fermentation condition such as substrate concentration, inoculum volume, incubation time, moistening agent, inducers and organic and inorganic nitrogen sources was studied for enzyme production. Culture conditions were optimized for maximal yield of enzyme. The solid substrate wheat bran was most suitable for pectic enzyme production under SSF. Enzyme production was found maximum after 10 days of incubation. Lactose was found to be most effective as inducer. Gelatin as organic nitrogen source and ammonium nitrate as inorganic nitrogen source yielded high enzyme titres.

scholarly journals Valor nutritivo de la pulpa de café sometida a fermentación sólida usando Aspergillus niger en pollos y cerdos

2016 ◽  
Vol 1 ◽  
pp. 79 ◽  
Author(s):  
Mario Molina ◽  
Otto Raúl Lechuga ◽  
Ricardo Bressani
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Feed Efficiency ◽  
Nutritional Value ◽  
Fermentation Process ◽  
Initial Moisture Content ◽  
Original Material ◽  
Coffee Pulp ◽  
True Protein

Coffee pulp was subjected to a solid-state fermentation process using Aspergillus niger, an Initial moisture content of 80%, pH 3.5,35°C and adding 2.5% commercial urea and 2.0% commercial dicalcium phosphate, for a total of 48 h. The sundried fermented material proved to have a significantly (P<0.05) lower polyphenolic, caffeine and fiber content than the original sun-dried coffee pulp. Further, the true protein content of the fermented material (18%) was significantly (P<0.01) higher than that of the original material (5%). When the pulp was included at a 5, 10 and 15% levels In growing chicken rations It was found that after six weeks the ration containing 15% of the fermented pulp presented a weight gain (1.43 kg) and a feed efficiency (2.20) significantly equal to the control ration (without pulp) and better (P<0.05) than the ration containing the sundried, unfermented pulp at 15 % (1.19 kg and 2.55, respectively). In the case of growing swine fed with rations containing either fermented or unfermented pulp at 20% level for 8 weeks the results were similar to those obtained In the chicken experiment. It is concluded that solid-state fermentation represents a viable technological alternative to improve the nutritional value of coffee pulp.

Hydrolytic enzyme production in solid-state fermentation by Aspergillus niger 3T5B8

2000 ◽  
Vol 36 (3) ◽  
pp. 255-261 ◽  
Author(s):  
Sonia Couri ◽  
Selma da Costa Terzi ◽  
Gustavo A Saavedra Pinto ◽  
Suely Pereira Freitas ◽  
Antonio Carlos Augusto da Costa
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Hydrolytic Enzyme

scholarly journals Food Waste-Assisted Metal Extraction from Printed Circuit Boards: The Aspergillus niger Route

2021 ◽  
Vol 9 (5) ◽  
pp. 895
Author(s):  
Carlotta Alias ◽  
Daniela Bulgari ◽  
Fabjola Bilo ◽  
Laura Borgese ◽  
Alessandra Gianoncelli ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Food Waste ◽  
Solid State Fermentation ◽  
Printed Circuit Boards ◽  
Electronic Waste ◽  
Metal Extraction ◽  
Circuit Boards ◽  
Printed Circuit ◽  
Waste Printed Circuit Boards

A low-energy paradigm was adopted for sustainable, affordable, and effective urban waste valorization. Here a new, eco-designed, solid-state fermentation process is presented to obtain some useful bio-products by recycling of different wastes. Urban food waste and scraps from trimmings were used as a substrate for the production of citric acid (CA) by solid state fermentation of Aspergillus niger NRRL 334, with a yield of 20.50 mg of CA per gram of substrate. The acid solution was used to extract metals from waste printed circuit boards (WPCBs), one of the most common electronic waste. The leaching activity of the biological solution is comparable to a commercial CA one. Sn and Fe were the most leached metals (404.09 and 67.99 mg/L, respectively), followed by Ni and Zn (4.55 and 1.92 mg/L) without any pre-treatments as usually performed. Commercial CA extracted Fe more efficiently than the organic one (123.46 vs. 67.99 mg/L); vice versa, biological organic CA recovered Ni better than commercial CA (4.55 vs. 1.54 mg/L). This is the first approach that allows the extraction of metals from WPCBs through CA produced by A. niger directly grown on waste material without any sugar supplement. This “green” process could be an alternative for the recovery of valuable metals such as Fe, Pb, and Ni from electronic waste.

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