Endothelial integrity of ultrasonically skeletonized internal thoracic artery: morphological analysis with scanning electron microscopy

Abstract The morphology of the amorphous, gelatinous, and crystalline varieties of monoclonal IgG cryoglobulins was analyzed by light and transmission and scanning electron microscopy. Each cryoglobulin had a characteristic fine structure that correlated with its gross morphology. Transmission electron microscopy showed that the amorphous precipitates were random and disorganized molecular clumps. In contrast, cryogels were thin-walled, well-organized, and hydrated strawlike clusters, whereas cryocrystals formed tightly compacted, highly structured molecular clusters. Crystals that formed in blood produced rouleaux, and analysis by scanning electron microscopy indicated that the crystals could form thick-walled, branching, macromolecular nets that could physically trap cells. The morphological properties provided visual impressions by which cryoglobulins could cause clinical disease secondary to vascular occlusion produced by self- associated IgG cryoglobulin molecules.

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Luticola puchalskiana, a new small terrestrial Luticola species (Bacillariophyceae) from the Maritime Antarctic Region

Phytotaxa ◽

10.11646/phytotaxa.450.1.6 ◽

2020 ◽

Vol 450 (1) ◽

pp. 85-94

Author(s):

NATALIA KOCHMAN-KĘDZIORA ◽

RALITSA ZIDAROVA ◽

TERESA NOGA ◽

MARIA OLECH ◽

BART VAN DE VIJVER

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Morphological Analysis ◽

New Taxon ◽

Maritime Antarctic ◽

Antarctic Region ◽

Diatom Flora ◽

Terrestrial Habitats ◽

Two Populations ◽

Scanning Electron

During a survey of the terrestrial diatom flora of the Maritime Antarctic Region, an unknown Luticola taxon that could not be identified using the currently available literature was observed on two islands of the South Shetland Archipelago. After a detailed morphological analysis and comparison, the unknown taxon is described as Luticola puchalskiana sp. nov. The new species can be separated from other Luticola species based on its valve dimensions, stria density and the shape of both central and terminal raphe endings. Two populations of the new taxon were observed in samples collected from terrestrial habitats. The morphology of Luticola puchalskiana is elaborately described and illustrated using both light and scanning electron microscopy observations. A comparison is made with a large number of other morphologically similar Luticola species from the entire (sub)Antarctic Region.

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Six-Month Healing of the Nonocclusive Coronary Anastomotic Connector in an Off-Pump Porcine Bypass Model

Innovations Technology and Techniques in Cardiothoracic and Vascular Surgery ◽

10.1097/imi.0000000000000055 ◽

2014 ◽

Vol 9 (2) ◽

pp. 130-136 ◽

Cited By ~ 2

Author(s):

David Stecher ◽

Pierfrancesco Agostoni ◽

Gerard Pasterkamp ◽

Imo E. Hoefer ◽

Lex A. van Herwerden ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Optical Coherence Tomography ◽

Coronary Artery ◽

Pilot Study ◽

Internal Thoracic Artery ◽

Artery Bypass ◽

Optical Coherence ◽

Off Pump ◽

Scanning Electron

Objective This pilot study evaluates the anastomotic healing of the Excimer Laser Assisted Nonocclusive Anastomosis coronary connector at 6 months in a porcine off-pump coronary artery bypass (OPCAB) model. Methods Left internal thoracic artery to left anterior descending coronary artery bypass in two animals and left internal thoracic artery to left anterior descending coronary artery and right internal thoracic artery to right coronary artery bypasses in one animal were evaluated intraoperatively and at 6 months. The anastomoses (n = 4) were examined by angiography, intravascular ultrasound, optical coherence tomography, scanning electron microscopy, and histology. Results At follow-up, all anastomoses (n = 4) were fully patent (FitzGibbon grade A). Scanning electron microscopy demonstrated complete endothelial coverage of the anastomotic surface, and histology showed minimal streamlining intimal hyperplasia. The in vivo intravascular ultrasound and optical coherence tomography acquisitions confirmed histologic findings. Optical coherence tomography demonstrated 0.06-mm intimal coverage of the intraluminal part of the connector along the full circumference of the anastomosis. Conclusions In this pilot study, the Excimer Laser Assisted Non-occlusive Anastomosis coronary connector showed an excellent healing response on the long-term in the porcine OPCAB model. Hence, this new concept might be a potential alternative to hand-sutured anastomosis in (minimally invasive) OPCAB surgery.

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Early morphological and chemical changes induced by cisplatin in the guinea pig organ of Corti

The Journal of Laryngology & Otology ◽

10.1017/s0022215100101161 ◽

1986 ◽

Vol 100 (12) ◽

pp. 1375-1383 ◽

Cited By ~ 28

Author(s):

Spiro D. Comis ◽

Peter H. Rhys-Evans ◽

Michel P. Osborne ◽

James O. Pickles ◽

David J. R. Jeffries ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Single Dose ◽

Hair Cells ◽

Morphological Analysis ◽

Organ Of Corti ◽

Cytoplasmic Calcium ◽

X Ray ◽

High Calcium ◽

Scanning Electron

AbstractGuinea pigs were treated with a single dose of Cisplatin (5 mg IP). After 2–4 days the cochleas were prepared for morphological analysis by scanning electron microscopy and chemical analysis by X-ray dispersive microanalysis. Following Cisplatin, the bundles of stereocilia on the hair cells were found to be rough, disarrayed, fused, and finally absorbed. Significant increases were found in the levels of calcium, sulphur, and phosporus in the abnormal hair cells. It is suggested that the high calcium levels might be due to the inhibition of enzymes which normally keep cytoplasmic calcium low, and that some of the changes in the stereocilia might be secondary to this.

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The Effect of Different Silica Compositions to the Properties of Silica Foam Fabricated Using Slurry Method

Materials Science Forum ◽

10.4028/www.scientific.net/msf.888.121 ◽

2017 ◽

Vol 888 ◽

pp. 121-125 ◽

Cited By ~ 1

Author(s):

Syazwani Baharom ◽

Sufizar Ahmad ◽

Muhamad Izranuddin Mohd Ramli

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Compressive Strength ◽

Polyethylene Glycol ◽

Morphological Analysis ◽

Strength Test ◽

Distilled Water ◽

Compressive Strength Test ◽

Slurry Method ◽

Scanning Electron

Silica foams with 50% – 70% porosity have been developed by mixing silica powder, polyethylene glycol (PEG) and carboxymethyl cellulose (CMC) into distilled water to make slurry. Polyurethane foam (PU) as the template is immersed into the slurry with 45 wt.%, 50 wt.% and 55 wt.%. of silica composition and finally sintered at 1100°C, 1200°C, 1300°C and 1400°C. Several tests which are morphological analysis, porosity and density test, and compressive strength test are compulsory to determine the physical and mechanical of the silica foam. The morphology of the foam has been observed using Scanning Electron Microscopy (SEM) and the result of pore size distribution is in the range of 255.91 μm to 489.14 μm. The porosity and density obtained from the porosity and density test was 50%-73.66% and 0.5499 g/cm³ up to 0.9757 g/cm³, respectively. Meanwhile, the compressive strength of sintered silica foam obtained is 0.01471 N/mm² up to 0.1467 N/mm².

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Study of Morphology of Sorption Substances

Drug development & registration ◽

10.33380/2305-2066-2019-8-2-33-37 ◽

2019 ◽

Vol 8 (2) ◽

pp. 33-37 ◽

Cited By ~ 2

Author(s):

A. V. Bondarev ◽

E. T. Zhilyakova ◽

N. B. Demina ◽

V. Y. Novikov

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Silicon Dioxide ◽

Morphological Analysis ◽

Spatial Organization ◽

Montmorillonite Clay ◽

Drug Molecule ◽

Levels Of Organization ◽

Active Coal ◽

Scanning Electron

Introduction. Substances with sorption properties can be used to create transport drug systems, in which the main mechanism of binding, transport and release of the drug molecule is sorption. The sorbent in this case acts as a carrier of the drug molecule, followed by its delivery to the destination by desorption. One of the ways to study the processes of sorption-desorption in transport drug systems is the study of the morphology of the sorption substance. Therefore, the morphological analysis of sorption substances is important, including the size, shape, and spatial organization of their structural elements.Aim. The study of the morphology of sorption substances.Materials and methods. The materials of the study are active coal, silicon dioxide, povidone, dioctahedral smectite, kaolin and montmorillonite clay. The methods is scanning electron microscopy.Results and discussion. The scanning electron microscopy of objects was carried out using segmentation of elements as subsystems, inside of which the morphological description does not penetrate. It was established that for coal of active and silicon dioxide, the segmentation of elements is represented by three levels of organization; for povidone, smectite, kaolin and montmorillonite clay, the segmentation of elements is represented by two levels of organization. The morphology of the objects was investigated. It is established that the studied substances are microstructural objects. Porosity in samples of active coal, smectite dioctahedral, kaolin, montmorillonite clay was determined. In samples of silicon dioxide and povidone porosity is absent.Conclusion. Morphological analysis of sorption substances allowed us to develop classification of the possible interaction of the carrier substance with the drug molecule in the transport drug system. The materials under study are divided into two groups according to porous characteristics: group 1 – porous substances – sorption interaction in pores (active coal), sorption interaction in pores and by ion exchange (smectite, montmorillonite clay), sorption in secondary pores and through oxygen and hydroxyl centers (kaolin); group 2 – non-porous substances – sorption on oxygen centers (silicon dioxide), sorption by means of complex formation (povidone). The prospect of further research is the modeling of porosity and sorption interaction of the carrier substance with the drug molecule in the drug transport system.

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