Performance of self-sampled HPV test in comparison with liquid based cytology

Objective: to evaluate the eff ectiveness of the regional cervical cancer screening program by liquid-based cytology. Materials and Methods: the geography of screening includes 54 medical units of the Rostov region, where patients are taken swabs from the cervix. HPV positive patients with abnormal PAP smears are invited to the Regional cervical pathology center for diagnosis and treatment. Methods. liquid-based cytology, the HPV test RealTime PCR, colposcopy with video recording, biopsy of the cervix by the method of loop excision or electroradiographic conization. Results: From 2014 to 2017, 188 641 cytology tests were performed. Cervical cancer was revealed in 189 women – (0.1 %), H-SIL and ASCH in 566 (0.3 %), 377 ASCUS (0.2 %), L-SIL in 23014 (12.2 %), absence of intracellular lesions or malignancy in 164495 (87.2 %). 2162 HPV-positive women aged 18 to 69 years were invited to the Regional cervical pathology center gynecologist for examination. Treatment by conization (622) and excision (830) of the cervix was performed in 1452 patients, H-SIL and CIS were revealed in 1162 women (78 %). Conclusion: tests such as liquid-based cytology and HPV testing, their optimal multiplicity and sequence allow timely detection and treatment of precancerous cervical epithelium lesions.

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HrHPV testing vs liquid-based cytology in cervical cancer screening among women aged 50 and older: a prospective study

International Journal of Gynecological Cancer ◽

10.1136/ijgc-2020-001457 ◽

2020 ◽

Vol 30 (11) ◽

pp. 1678-1683

Author(s):

Berit Andersen ◽

Sisse Helle Njor ◽

Anne Marie Schak Jensen ◽

Tonje Johansen ◽

Ulla Jeppesen ◽

...

Keyword(s):

Cervical Cancer ◽

Cancer Screening ◽

Prospective Study ◽

Cervical Cancer Screening ◽

Gynecological Cancer ◽

Dna Amplification ◽

Hpv Testing ◽

Promising Alternative ◽

Hpv Test ◽

Liquid Based Cytology

IntroductionEvidence supports high-risk human papilloma virus (HPV) testing as the primary cervical cancer screening tool. However, benefits and harms should be carefully considered before replacing liquid-based cytology. In women age 50 and older, we evaluated how a commercially available DNA amplification HPV test compares with routine liquid-based cytology.MethodsThis prospective study included 4043 patients who had a cervical sample analyzed from September 2011 to September 2012. Patients were followed between 64 and 76 months (median: 70 months). Samples were analyzed using both liquid-based cytology and the Cobas 4800 HPV DNA test. We calculated the diagnostic efficacy of liquid-based cytology and HPV, with or without the opposite test as triage, using cervical intraepithelial neoplasia (CIN2+/CIN3+) as reference.ResultsThe patients had a median age of 58 years, (range; 50–90). At baseline, HPV prevalence was 8.0%: a total of 3.7% of patients had atypical squamous cells of undetermined significance or worse (ASCUS+). Positive test results were 1.9% for liquid-based cytology with HPV triage and 3.0% for HPV with liquid-based cytology triage. The cumulative incidence of CIN3+ was 1.0% (40/4043). Sensitivities for CIN3+ were: liquid-based cytology 47.5% (31.5%–63.9%); liquid-based cytology with HPV triage 45.0% (29.3%–61.5%); HPV 90.0% (76.3%–97.2%); and HPV with liquid-based cytology triage 67.5% (50.9%–81.4%). Corresponding specificities were: liquid-based cytology 96.6% (96.0%–97.2%); liquid-based cytology with HPV triage 98.5% (98.0%–98.8%); HPV 92.8% (92.0%–93.6%); and HPV with liquid-based cytology triage 97.7% (97.2%–98.1%). At baseline, HPV testing overlooked five cases of gynecological cancer other than cervical cancer. Five cervical cancers were detected, two had been overlooked at baseline by liquid-based cytology and two by HPV testingConclusionHPV screening using DNA amplification is a promising alternative to liquid-based cytology in women age 50 and older, but evaluation of alternative triage methods is warranted. The risk of overlooking cancers needs consideration when replacing liquid-based cytology with HPV testing as a method for primary screening.

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Follow-up Outcomes of a Large Cohort of Low-Risk Women With Negative Imaged Liquid-Based Cytology and Negative HPV Test Results

American Journal of Clinical Pathology ◽

10.1309/ajcp4df7aclbffgy ◽

2013 ◽

Vol 139 (1) ◽

pp. 32-38 ◽

Cited By ~ 11

Author(s):

Chengquan Zhao ◽

Baoying Weng ◽

Zaibo Li ◽

Huaitao Yang ◽

R. Marshall Austin

Keyword(s):

Large Cohort ◽

Low Risk ◽

Test Results ◽

Hpv Test ◽

Liquid Based Cytology ◽

Low Risk Women

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Cost-effectiveness of liquid-based cytology with or without hybrid-capture II HPV test compared with conventional Pap smears: A study by the French society of clinical cytology

Diagnostic Cytopathology ◽

10.1002/dc.20283 ◽

2005 ◽

Vol 33 (5) ◽

pp. 338-343 ◽

Cited By ~ 14

Author(s):

Béatrix Cochand-Priollet ◽

Isabelle Cartier ◽

Patricia de Cremoux ◽

Catherine Le Galès ◽

Marianne Ziol ◽

...

Keyword(s):

Cost Effectiveness ◽

Pap Smears ◽

French Society ◽

Hybrid Capture ◽

Hpv Test ◽

Liquid Based Cytology

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Accuracy of p16/Ki-67 dual staining for improved cervical cancer risk stratification in women aged ≤30 years with hr-HPV positive

10.21203/rs.3.rs-91126/v1 ◽

2020 ◽

Author(s):

Xingmin Wang ◽

Xindan Wang ◽

Ping Wu ◽

Jinjian Fu ◽

Guixian Yi ◽

...

Keyword(s):

Cervical Cancer ◽

Predictive Value ◽

Precancerous Lesion ◽

Hpv Infection ◽

Effective Control ◽

Ki67 Expression ◽

Hpv Test ◽

Liquid Based Cytology ◽

Positive Population ◽

Dual Staining

Abstract Background: Cervical cancer (CC) is the most common malignancy inwomen on the earth. Cervical cancer usually develops from cervical intraepithelial neoplasia (CIN) grade 1 or above. Early detection of CIN1 or above precancerous lesion can effective control of cervical cancer incidence. The goal of this study was to evaluate the accuracy of p16/Ki67 dual staining in triaging hr-HPV positive population aged ≤ 30 years. Methods: A total of 336 women with an average age of 27.8 years old were included in this study. Liquid based cytology (LBC) samples were detected by p16/Ki67 immunocytochemical dual staining, liquid-based cytology, high-risk human papillomavirus (hr-HPV) and HPV 16/18 test. Diagnosis of each method was verified by histopathological test. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and AUC (area under the receiver operating characteristic curve, ROC curve) was obtained. Results: All assays had a high sensitivity for the detection of CIN2+. p16/Ki67 dual staining had similar sensitivity with hr-HPV test for CIN2+ detection (89.9% vs 93.9%, P = 0.781), and had similar sensitivity with LBC test (89.9% vs 82.7%, P=0.588). However, p16/Ki67 dual staining had higher specificity than that of both hr-HPV test (70.1% vs 25.5%, P<0.001) and LBC test (70.1% vs 38.9%, P=0.002) for CIN2+ detection. p16/Ki67 dual staining had bigger AUC (0.80) than that of hr-HPV test (0.60) and LBC test (0.61), the P value was 0.002, 0.003, respectively. The specificity of dual staining for CIN2+ detection in hr-HPV positive women was 70.1%, which was higher than that of LBC test (41.9%, P=0.020). Colposcopy referral rate of p16/Ki67 was lower than that of both hr-HPV and LBC (P=0.002, <0.001, respectively). HPV infection was significantly associated with p16/Ki67 expression in all patients. p16/Ki67 expression in HPV16/18 and other 15 types of hr-HPV infection was significantly higher than negative hr-HPV infection, odds ratio (OR) was 12.16 (95% confidence interval, 95% CI = 5.82-25.41) and 2.65 (95% CI = 1.27-5.51), respectively. Conclusions: p16/Ki67 dual staining has a good specificity of high-grade cervical lesions detection and is a promising tool in triage of CIN2+ and hr-HPV positive population and avoid over diagnosis and treatment.

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Cost-Effectiveness of Liquid-Based Cytology with or without Hybrid-Capture II HPV Test Compared with Conventional Pap Smears

Journal of Lower Genital Tract Disease ◽

10.1097/01.lgt.0000210132.46758.df ◽

2006 ◽

Vol 10 (2) ◽

pp. 129

Author(s):

B Cochand-Priollet ◽

I Cartier ◽

P de Cremoux ◽

C Le Gales ◽

M Ziol ◽

...

Keyword(s):

Cost Effectiveness ◽

Pap Smears ◽

Hybrid Capture ◽

Hpv Test ◽

Liquid Based Cytology

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Analysis of the efficacy of liquid-based cytology combined with HPV genotypes in screening cervical lesions in women of different ages

Journal of Laboratory Medicine ◽

10.1515/labmed-2019-0144 ◽

2020 ◽

Vol 44 (3) ◽

pp. 151-156

Author(s):

Jun Jin ◽

Chao-Yan Yue

Keyword(s):

Positive Predictive Value ◽

Negative Predictive Value ◽

Predictive Value ◽

Age Groups ◽

Age Group ◽

Combined Method ◽

Predictive Values ◽

Hpv Test ◽

Hpv Genotypes ◽

Liquid Based Cytology

Abstract Objectives To evaluate the value of liquid-based cell examination combined with human papillomavirus (HPV) genotypes in the detection of cervical intraepithelial neoplasia. Methods A total of 21,155 patients who had undergone cytology, HPV genotypes and colposcopy-guided biopsy were grouped into three groups. Sensitivity, specificity, positive predictive value, and negative predictive value of the test methods of the three groups were compared. Results In the age group of less than 50 years, the sensitivity of HPV genotype testing was significantly higher than that of liquid-based cytology. The specificity of liquid-based cytology was significantly higher in all age groups than that of HPV genotype testing. The positive predictive value of each test index was higher in the age group less than 30 years, and the negative predictive value was higher in the 30–50-year age groups. In different age groups, liquid-based cytology and HPV genotype combined method can improve the sensitivity of detection and negative predictive values. Conclusions The HPV test has higher sensitivity and negative predictive value, and the cytology method is relatively specific and less sensitive. The combined method has better sensitivity and negative predictive value.

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Incidence of Positive HPV High Risk in Negative Cytology Result

Indonesian Journal of Obstetrics and Gynecology ◽

10.32771/inajog.v7i4.1158 ◽

2019 ◽

pp. 244-250

Author(s):

Junita Indarti ◽

Darrell Fernando ◽

Finish Fernando ◽

Ribkhi A. Putri ◽

Anggara Mahardika ◽

...

Keyword(s):

Cervical Cancer ◽

High Risk ◽

Dna Test ◽

Hpv Dna ◽

Hpv Test ◽

Significant Difference ◽

Flow Through ◽

Liquid Based Cytology ◽

High Risk Hpv ◽

Cytology Result

Abstract Objective: This research aim is to report the incidence of positive HPV high risk in negative cytology result. Method: We collected 83 women underwent liquid-based cytology (LBC) and HPV DNA examination at the same time. We were using DiagCor GenoFlow Human Papillomavirus Array Test (GenoFlow), a novel HPV test based on PCR and "Flow-through" hybridization that can identify 33 HPV subtypes: 18 types of High risk HPV such as 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 81 and 82 Result: We grouped the subjects based on age below or equal to 30 years old (n=6) and above 30 years old (n=77). We found a significant difference in HPV DNA result within this group (P = 0.034), with 19.3% had HPV DNA type 16 and 18 in a group of age above 30 years old. Our study showed that 27 women (32.5%) underwent screening for cervical cancer having negative LBC result but showed positive HPV DNA positive. Conclusion: We found a significant difference in HPV DNA test result among women above 30 years old. Co-testing of Pap and HPV DNA is needed, especially if HPV DNA type 16 and 18 were found among negative Pap results. Keywords: Cervical cancer, HPV DNA, incidence, LBC, screening Abstrak Tujuan: Penelitian ini bertujuan melaporkan insidensi dari HPV risiko tinggi yang positif pada hasil sitologi negatif. Metode: Kami mengumpulkan 83 wanita yang menjalani liquid based cytology (LBV) dan pemeriksaan HPV DNA pada waktu yang bersamaan. Dengan menggunakan DiagCor GenoFlow Human Papilloma Virus Array Test (GenoFlow), yaitu sebuah uji HPV terbaru yang berbasis PCR dan “Flow-throug” hybridization dapat mengidentifikasi 33 subtipe HPV: 18 tipe HPV risiko tinggi seperti 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 81 dan 82. Hasil: Kami mengelompokkan subjek berdasarkan usia dibawah atau setara 30 tahun dan diatas 30 tahun (n=77). Kami menemukan perbedaansignifikan dari hasil HPV DNA dalam kelompok ini (P=0.034), dengan 19.3% memiliki HPV DNA tipe 16 dan 18 dalam kelompok usia diatas 30 tahun. Penelitian ini menunjukkan 27 perempuan (32.5%) menjalani skrining kanker serviks memiliki hasil LBC yang negatif namun menunjukkan HPV DNA positif. Kesimpulan: Terdapat perbedaan signifikan dari uji HPV DNA pada wanita usia diatas 30 tahun. Pemeriksaan bersamaan antara Pap dan HPV DNA dibutuhkan terutama ketika HPV DNA tipe 16 dan 18 ditemuka pada hasil Pap negatif. Kata kunci: HPV DNA, insidensi, kanker serviks, LBC, skrining

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