Experimental colitis models: Insights into the pathogenesis of inflammatory bowel disease and translational issues

2015 ◽  
Vol 759 ◽  
pp. 253-264 ◽  
Author(s):  
Vassilis Valatas ◽  
Giorgos Bamias ◽  
George Kolios
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Experimental Colitis ◽  
Inflammatory Bowel

Changes in mucosal fatty acid profile in inflammatory bowel disease and in experimental colitis: a common response to bowel inflammation

1997 ◽  
Vol 16 (4) ◽  
pp. 177-183 ◽  
Author(s):  
F. Fernández-Bañares ◽  
M. Esteve-Comas ◽  
J. Mañé ◽  
E. Navarro ◽  
X. Bertrán ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Bowel Disease ◽  
Experimental Colitis ◽  
Common Response ◽  
Inflammatory Bowel

scholarly journals ErbB-1 experssion in experimental model of inflammatory bowel disease in rats

2004 ◽  
Vol 51 (2) ◽  
pp. 85-89 ◽  
Author(s):  
R. Trzcinski ◽  
M. Bry ◽  
W. Krajewska ◽  
M. Kulig ◽  
A. Dzyiki
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Intestinal Inflammation ◽  
Growth Factor Receptor ◽  
Experimental Colitis ◽  
Altered Level ◽  
Pcr Products ◽  
Epidermal Growth ◽  
Inflammatory Bowel ◽  
Specific Symptoms

Background Ulcerative colitis (UC) and Crohn?s disease (CD) belong to inflammatory bowel disease (IBD). The etiology of IBD is still unknown. Therapy remains empiric or is used for the relief of specific symptoms. The erbB-1 oncogene coding epidermal growth factor receptor (EGFR) is typed as a prognostic marker in several benign and malignant tissues. The aim of our study was to examine the erbB-1 expression in experimental surgically performed model of IBD in rats and to find out if there is any correlation between severity of the intestinal inflammation and altered level of erbB-1 expression. After inducing an experimental colitis samples were taken from different parts of the intestine in all studied groups of rats for histopathology. ErbB-1 mRNA expression was estimated by RT-PCR. PCR products were separated on a 1,5% TBE-agarose gel and visualized with ethidium bromide. The integrated optical density (IOD) of electrophoretically separated amplification products was measured using a video densitometer and Gel-Pro 3.0 software. Nonparametrical statistical test has been used throughout in analyzing the results. Relative erbB-1 expression was determined by comparing to cyclophiline expression. Results Microscopic changes were similar to those observed in IBD. ErbB-1 expression was significantly higher in inflamed tissues of the bowel ( P=0.04 for the transverse colon and P=0.027 for the cecum). Significantly higher erbB-1 expression in inflamed tissues of the bowel suggests that EGFR overexpression may play a role in the pathogenesis of IBD. Overexpression of erbB-1 correlates with the severity of inflammation in bowel tissues.

scholarly journals PPARγin Inflammatory Bowel Disease

PPAR Research ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Vito Annese ◽  
Francesca Rogai ◽  
Alessia Settesoldi ◽  
Siro Bagnoli
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Potential Role ◽  
Intestinal Inflammation ◽  
Experimental Colitis ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Beneficial Effects ◽  
Inflammatory Bowel

Peroxisome proliferator-activated receptor gamma (PPARγ) is member of a family of nuclear receptors that interacts with nuclear proteins acting as coactivators and corepressors. The colon is a major tissue which expresses PPARγin epithelial cells and, to a lesser degree, in macrophages and lymphocytes and plays a role in the regulation of intestinal inflammation. Indeed, both natural and synthetic PPARγligands have beneficial effects in different models of experimental colitis, with possible implication in the therapy of inflammatory bowel disease (IBD). This paper will specifically focus on potential role of PPARγin the predisposition and physiopathology of IBD and will analyze its possible role in medical therapy.

scholarly journals Curcumin Regulated the Homeostasis of Memory T Cell and Ameliorated Dextran Sulfate Sodium-Induced Experimental Colitis

2021 ◽  
Vol 11 ◽  
Author(s):  
You-Bao Zhong ◽  
Zeng-Ping Kang ◽  
Bu-Gao Zhou ◽  
Hai-Yan Wang ◽  
Jian Long ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Peripheral Blood ◽  
Dextran Sulfate ◽  
Dextran Sulfate Sodium ◽  
Experimental Colitis ◽  
Immune Memory ◽  
Effector Memory ◽  
Colon Tissue ◽  
Inflammatory Bowel

Immune memory is protective against reinvasion by pathogens in the homeostatic state, while immune memory disorders can cause autoimmune disease, including inflammatory bowel disease. Curcumin is a natural compound shown to be effective against human inflammatory bowel disease and experimental colitis, but the underlying mechanism is unclear. Here, experimental colitis was induced by dextran sulfate sodium (DSS) in this study. Significant changes in the percentages of naïve, central memory T (TCM), and effector memory (TEM) cells and their CD4+ and CD8+ subsets were found in the peripheral blood of mice with colitis using flow cytometry. After 7 days of continuous curcumin (100 mg/kg/day) administration, the DSS-induced experimental colitis was effectively relieved, with significant decreases in the ratio of day weight to initial body weight, colonic weight, pathological injury score, levels of proinflammatory cytokines IL-7, IL-15, and IL-21, colonic mucosal ulceration, and amount of inflammatory infiltrate. Importantly, curcumin significantly restored the percentages of naïve, TCM, and TEM cells and their CD4+ and CD8+ subpopulations. In addition, curcumin significantly inhibited the activation of the JAK1/STAT5 signaling pathway, downregulation of JAK1, STAT5, and p-STAT5 proteins in colon tissue, and upregulation of PIAS1 proteins. These results suggested that curcumin effectively regulated the differentiation of naïve, TCM, and TEM cells in the peripheral blood to alleviate DSS-induced experimental colitis, which might be related to the inhibition of JAK1/STAT5 signaling activity.

Protective effects of bioactive peptides in foxtail millet protein hydrolysates against experimental colitis in mice

2022 ◽  
Author(s):  
Bowei Zhang ◽  
Yingchuan Xu ◽  
Congying Zhao ◽  
Yunhui Zhang ◽  
Huan Lv ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Bioactive Peptides ◽  
Dietary Intervention ◽  
Foxtail Millet ◽  
Intervention Strategy ◽  
Experimental Colitis ◽  
Protective Effects ◽  
Active Ingredients ◽  
Inflammatory Bowel

It is of great significance to develop a dietary intervention strategy to prevent inflammatory bowel disease (IBD). A millet-rich diet can ameliorate IBD, but the active ingredients and mechanisms remain...

scholarly journals Application of a chemical probe to detect neutrophil elastase activation during inflammatory bowel disease

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Bethany M. Anderson ◽  
Daniel P. Poole ◽  
Luigi Aurelio ◽  
Garrett Z. Ng ◽  
Markus Fleischmann ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Legionella Pneumophila ◽  
Bowel Disease ◽  
Translational Control ◽  
Neutrophil Elastase ◽  
Experimental Colitis ◽  
Dependent Manner ◽  
Elastase Activity ◽  
Accurate Identification ◽  
Inflammatory Bowel

Abstract Neutrophil elastase is a serine protease that has been implicated in the pathogenesis of inflammatory bowel disease. Due to post-translational control of its activation and high expression of its inhibitors in the gut, measurements of total expression poorly reflect the pool of active, functional neutrophil elastase. Fluorogenic substrate probes have been used to measure neutrophil elastase activity, though these tools lack specificity and traceability. PK105 is a recently described fluorescent activity-based probe, which binds to neutrophil elastase in an activity-dependent manner. The irreversible nature of this probe allows for accurate identification of its targets in complex protein mixtures. We describe the reactivity profile of PK105b, a new analogue of PK105, against recombinant serine proteases and in tissue extracts from healthy mice and from models of inflammation induced by oral cancer and Legionella pneumophila infection. We apply PK105b to measure neutrophil elastase activation in an acute model of experimental colitis. Neutrophil elastase activity is detected in inflamed, but not healthy, colons. We corroborate this finding in mucosal biopsies from patients with ulcerative colitis. Thus, PK105b facilitates detection of neutrophil elastase activity in tissue lysates, and we have applied it to demonstrate that this protease is unequivocally activated during colitis.

Sign in / Sign up

Export Citation Format

Share Document