Antioxidant enzymatic activities and gene expression associated with heat tolerance in a cool-season perennial grass species

2013 ◽  
Vol 87 ◽  
pp. 159-166 ◽  
Author(s):  
Hongmei Du ◽  
Peng Zhou ◽  
Bingru Huang
Keyword(s):  
Gene Expression ◽  
Heat Tolerance ◽  
Perennial Grass ◽  
Enzymatic Activities ◽  
Grass Species ◽  
Cool Season

scholarly journals Characterization of Gene Expression Associated with Drought Avoidance and Tolerance Traits in a Perennial Grass Species

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e103611 ◽  
Author(s):  
Peng Zhou ◽  
Yuan An ◽  
Zhaolong Wang ◽  
Hongmei Du ◽  
Bingru Huang
Keyword(s):  
Gene Expression ◽  
Perennial Grass ◽  
Grass Species ◽  
Drought Avoidance

scholarly journals Natural variation of physiological traits, molecular markers, and chlorophyll catabolic genes associated with heat tolerance in perennial ryegrass accessions

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Jing Zhang ◽  
Hui Li ◽  
Yiwei Jiang ◽  
Huibin Li ◽  
Zhipeng Zhang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Heat Tolerance ◽  
Perennial Ryegrass ◽  
Physiological Traits ◽  
Grass Species ◽  
Cool Season ◽  
Catabolic Genes ◽  
Heat Tolerant ◽  
Cool Season Grass

Abstract Background Identification of genetic diversity in heat tolerance and associated traits is of great importance for improving heat tolerance in cool-season grass species. The objectives of this study were to determine genetic variations in heat tolerance associated with phenotypic and physiological traits and to identify molecular markers associated with heat tolerance in a diverse collection of perennial ryegrass (Lolium perenne L.). Results Plants of 98 accessions were subjected to heat stress (35/30 °C, day/night) or optimal growth temperature (25/20 °C) for 24 d in growth chambers. Overall heat tolerance of those accessions was ranked by principal component analysis (PCA) based on eight phenotypic and physiological traits. Among these traits, electrolyte leakage (EL), chlorophyll content (Chl), relative water content (RWC) had high correlation coefficients (− 0.858, 0.769, and 0.764, respectively) with the PCA ranking of heat tolerance. We also found expression levels of four Chl catabolic genes (CCGs), including LpNYC1, LpNOL, LpSGR, and LpPPH, were significant higher in heat sensitive ryegrass accessions then heat tolerant ones under heat stress. Furthermore, 66 pairs of simple sequence repeat (SSR) markers were used to perform association analysis based on the PCA result. The population structure of ryegrass can be grouped into three clusters, and accessions in cluster C were relatively more heat tolerant than those in cluster A and B. SSR markers significantly associated with above-mentioned traits were identified (R2 > 0.05, p < 0.01)., including two pairs of markers located on chromosome 4 in association with Chl content and another four pairs of markers in association with EL. Conclusion The result not only identified useful physiological parameters, including EL, Chl content, and RWC, and their associated SSR markers for heat-tolerance breeding of perennial ryegrass, but also highlighted the involvement of Chl catabolism in ryegrass heat tolerance. Such knowledge is of significance for heat-tolerance breeding and heat tolerance mechanisms in perennial ryegrass as well as in other cool-season grass species.

scholarly journals Natural Variation of Physiological Traits, Molecular Markers, and Chlorophyll Catabolic Genes Associated With Heat Tolerance in Perennial Ryegrass Accessions

2020 ◽  
Author(s):  
Bin Xu ◽  
Jing Zhang ◽  
Hui Li ◽  
Yiwei Jiang ◽  
Huibin Li ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Heat Tolerance ◽  
Perennial Ryegrass ◽  
Principal Component ◽  
Physiological Traits ◽  
Grass Species ◽  
Cool Season ◽  
Catabolic Genes ◽  
Cool Season Grass

Abstract Background: Identification of genetic diversity in heat tolerance and associated traits is of great importance for improving heat tolerance in cool-season grass species. The objectives of this study were to determine genetic variations in heat tolerance associated with phenotypic and physiological traits and to identify molecular markers associated with heat tolerance in a diverse collection of perennial ryegrass (Lolium perenne L.). Results: Plants of 98 accessions were subjected to heat stress (35/30 ℃, day/night) or optimal growth temperature (25/20 ℃) for 24 d in growth chambers. Overall heattolerance of those accessions was ranked by principal component analysis (PCA) based on eight phenotypic and physiological traits. Among these traits, electrolyte leakage (EL), chlorophyll content (Chl), relative water content (RWC) had high correlation coefficients (−0.858, 0.769, and 0.764, respectively) with the PCA ranking of heat tolerance. We also found expression levels of four Chl catabolic genes (CCGs), including LpNYC1, LpNOL, LpSGR, and LpPPH, were closely related to the heat tolerance of ryegrass accessions. Furthermore, 66 pairs of simple sequence repeat (SSR) markers were used to perform association analysis based on the PCA result. The population structure of ryegrass can be grouped into three clusters, and accessions in cluster C were relatively more heat tolerant than those in cluster A and B. SSR markers significantly associated with above-mentioned traits were identified (R2>0.05, p<0.01)., including two pairs of markers located on chromosome 4 in association with Chl content and another four pairs of markers in association with EL.Conclusion:The result not only identified useful physiological parameters, including EL, Chl content, and RWC, and their associated SSR markers for heat-tolerance breeding of perennial ryegrass, but also highlighted the involvement of Chl catabolism in ryegrass heat tolerance. Such knowledge is of significance for heat-tolerance breeding and heat tolerance mechanisms in perennial ryegrass as well as in other cool-season grass species.

scholarly journals Natural variation of physiological traits, molecular markers, and chlorophyll catabolic genes associated with heat tolerance in perennial ryegrass accessions

2020 ◽  
Author(s):  
Jing Zhang ◽  
Hui Li ◽  
Yiwei Jiang ◽  
Huibin Li ◽  
Zhipeng Zhang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Heat Tolerance ◽  
Perennial Ryegrass ◽  
Physiological Traits ◽  
Grass Species ◽  
Cool Season ◽  
Catabolic Genes ◽  
Heat Tolerant ◽  
Cool Season Grass

Abstract Background: Identification of genetic diversity in heat tolerance and associated traits is of great importance for improving heat tolerance in cool-season grass species. The objectives of this study were to determine genetic variations in heat tolerance associated with phenotypic and physiological traits and to identify molecular markers associated with heat tolerance in a diverse collection of perennial ryegrass (Lolium perenne L.).Results: Plants of 98 accessions were subjected to heat stress (35/30 ℃, day/night) or optimal growth temperature (25/20 ℃) for 24 d in growth chambers. Overall heat tolerance of those accessions was ranked by principal component analysis (PCA) based on eight phenotypic and physiological traits. Among these traits, electrolyte leakage (EL), chlorophyll content (Chl), relative water content (RWC) had high correlation coefficients (−0.858, 0.769, and 0.764, respectively) with the PCA ranking of heat tolerance. We also found expression levels of four Chl catabolic genes (CCGs), including LpNYC1, LpNOL, LpSGR, and LpPPH, were significant higher in heat sensitive ryegrass accessions then heat tolerant ones under heat stress. Furthermore, 66 pairs of simple sequence repeat (SSR) markers were used to perform association analysis based on the PCA result. The population structure of ryegrass can be grouped into three clusters, and accessions in cluster C were relatively more heat tolerant than those in cluster A and B. SSR markers significantly associated with above-mentioned traits were identified (R2>0.05, p <0.01)., including two pairs of markers located on chromosome 4 in association with Chl content and another four pairs of markers in association with EL.Conclusion: The result not only identified useful physiological parameters, including EL, Chl content, and RWC, and their associated SSR markers for heat-tolerance breeding of perennial ryegrass, but also highlighted the involvement of Chl catabolism in ryegrass heat tolerance. Such knowledge is of significance for heat-tolerance breeding and heat tolerance mechanisms in perennial ryegrass as well as in other cool-season grass species.

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