Direct ethanol production from starch, wheat bran and rice straw by the white rot fungus Trametes hirsuta

2011 ◽  
Vol 48 (3) ◽  
pp. 273-277 ◽  
Author(s):  
Kenji Okamoto ◽  
Yasuyuki Nitta ◽  
Nitaro Maekawa ◽  
Hideshi Yanase
Keyword(s):  
Ethanol Production ◽  
Rice Straw ◽  
Wheat Bran ◽  
White Rot ◽  
White Rot Fungus ◽  
Trametes Hirsuta

scholarly journals Introducing a Thermo-Alkali-Stable, Metallic Ion-Tolerant Laccase Purified From White Rot Fungus Trametes hirsuta

2021 ◽  
Vol 12 ◽  
Author(s):  
Jing Si ◽  
Hongfei Ma ◽  
Yongjia Cao ◽  
Baokai Cui ◽  
Yucheng Dai
Keyword(s):  
Specific Activity ◽  
Endocrine Disrupting Chemicals ◽  
Environmental Pollutants ◽  
Fold Increase ◽  
Industrial Applications ◽  
White Rot ◽  
White Rot Fungus ◽  
Metallic Ions ◽  
Trametes Hirsuta ◽  
Ph Range

This study introduces a valuable laccase, designated ThLacc-S, purified from white rot fungus Trametes hirsuta. ThLacc-S is a monomeric protein in nature with a molecular weight of 57.0 kDa and can efficiently metabolize endocrine disrupting chemicals. The enzyme was successfully purified to homogeneity via three consecutive steps consisting of salt precipitation and column chromatography, resulting in a 20.76-fold increase in purity and 46.79% yield, with specific activity of 22.111 U/mg protein. ThLacc-S was deciphered as a novel member of the laccase family and is a rare metalloenzyme that contains cysteine, serine, histidine, and tyrosine residues in its catalytic site, and follows Michaelis-Menten kinetic behavior with a Km and a kcat/Km of 87.466 μM and 1.479 s–1μM–1, respectively. ThLacc-S exerted excellent thermo-alkali stability, since it was markedly active after a 2-h incubation at temperatures ranging from 20 to 70°C and retained more than 50% of its activity after incubation for 72 h in a broad pH range of 5.0–10.0. Enzymatic activities of ThLacc-S were enhanced and preserved when exposed to metallic ions, surfactants, and organic solvents, rendering this novel enzyme of interest as a green catalyst for versatile biotechnological and industrial applications that require these singularities of laccases, particularly biodegradation and bioremediation of environmental pollutants.

scholarly journals Identification of white rot fungus CP9 and its potential application in biopulping

2018 ◽  
Vol 14 (4) ◽  
pp. 721-726
Author(s):  
Nguyen Thi Hong Lien ◽  
Nguyen Van Hieu ◽  
Luong Thi Hong ◽  
Hy Tuan Anh ◽  
Phan Thi Hong Thao
Keyword(s):  
Rice Straw ◽  
Lignin Peroxidase ◽  
Paper Industry ◽  
Morphological Characteristics ◽  
White Rot Fungi ◽  
White Rot ◽  
White Rot Fungus ◽  
Wood Block ◽  
New Production ◽  
Cellulosic Fibers

Wood-rotting fungi represent an important component of forest ecosystems. Among them, white-rot fungi are the most efficient lignin degraders. Biopulping using white-rot fungi in pretreatment of the materials, is one of the solutions to overcome disadvantages of traditional production methods. Today, the isolation and screening of lignin degrading fungi capable for application in biopulping are of keen interest in Vietnam. The use of non–wood, plant fibres in pulp and paper industry, special, agricultural residuces such as rice and wheat straw, sugarcane baggase, cornstalks etc is the new production toward, potential, serving sustainable development. The fungus CP9, which possessed high ligninolytic activity, was identified and studied in pretreatment of rice straw for biopulping. The fruiting bodies of strain CP9 were effuse on trunk. The hymenium was porous and brown white with short tubes, the white mycelia penetrated wood block. The colony was off-white, blossom, irregularly circular. The mycelia were thick and closely bound together. Beside lignin, this fungus could degrade other substrates such as casein, carboxymethyl cellulose and starch. Biological and morphological characteristics of the fungus CP9 suggested its placement in subdivision Basidiomycota. Combined with the results of phylogenetic analysis, which showed 99% similarity of the fungus with species Leiotrametes lactinea, our strain was named as Leiotrametes lactinea CP9. This fungus could grow well on rice straw under solid state fermentation. Pretreatment of rice straw using L. lactinea CP9 was based on the activity of fungal lignin peroxidase and laccase. After 20 days, the residual enzyme activity was of 21.6 and 18.4 nkat/g material for lignin peroxidase and laccase, respectively. Pretreatment significantly improved the quality of straw, as lignin loss of 38% while cellulosic fibers were comparatively well preserved.

scholarly journals Biomechanical Pulping of Corn Stalk Rind with a White Rot Fungus – Trametes hirsuta – and the Use of Delignified Corn Stalk Pith as a Pulp Additive

BioResources ◽  
2015 ◽  
Vol 10 (4) ◽  
Author(s):  
Fangfang Wang ◽  
Honglei Chen ◽  
Mingqiang Ai ◽  
Yuzhong Zhang ◽  
Peiji Gao ◽  
...  
Keyword(s):  
White Rot ◽  
White Rot Fungus ◽  
Corn Stalk ◽  
Trametes Hirsuta

Degradation of endosulfan and endosulfan sulfate by white-rot fungus Trametes hirsuta

2011 ◽  
Vol 57 (4) ◽  
pp. 317-322 ◽  
Author(s):  
Ichiro Kamei ◽  
Kazuhiro Takagi ◽  
Ryuichiro Kondo
Keyword(s):  
White Rot ◽  
White Rot Fungus ◽  
Endosulfan Sulfate ◽  
Trametes Hirsuta

scholarly journals Characteristics of Compost from Balinese Cattle Dung (CD) and Rice Straw (RS) using White Rot Fungus (Wrf) (Ganoderma sp) As Bioactivators

2020 ◽  
Vol 15 (3) ◽  
pp. 194-204
Author(s):  
Muhammad Irfan Said ◽  
Jamila Mustabi ◽  
Siti Amelia Putri Syamsuddin
Keyword(s):  
Rice Straw ◽  
Ph Value ◽  
White Rot ◽  
White Rot Fungus ◽  
Cattle Dung ◽  
Randomized Design ◽  
Test Parameters ◽  
The Difference ◽  
The Republic ◽  
Range Of Values

To maximize the performance of the fermentation process, there is a need for a biactivator. The purpose of this study was to evaluate the characteristics of compost-based waste from cattle dung (CD) combined with rice straw (RS) by applying white rot fungus (Wrf) (Ganoderma sp) as a bioactivator. In this study, 6 types of treatments have been applied, namely T1(CD:RS)(25%:75%)+5% of Wrf; T2(CD:RS)(50%:50%)+5% of Wrf; T3(CD:RS)(75%:25%)+5% of Wrf; T4 (CD:RS)(25%:25%)+0% of Wrf; T5(CD:RS)(50%:50%)+0% of Wrf; T6 (CD:RS)(75%:25%)+0% of Wrf. The study was designed experimentally using a completely randomized design (CRD), 6 treatments with 3 replications. Based on the research results, the difference in the ratio of CD to RS and the use of Wrf bioactivator has a significant effect (p<0.05) in increasing the pH, temperature, C-organic and C/N ratio in compost. However, this difference had no significant effect (p>0.05) on the C-organic value. The pH value of compost was obtained with variations of 5.50±0.43-6.73±0.06. Temperature of compost is in the range of values 24.00±0.00-25.67 ±0.58 ºC. The C-organic value is in the range of 24.38±0.48-31.32±0.80%, while the N-organic value varies at 1.34±0.11-2.10±0.15%. The C/N ratio is in the range of 15.00± 0.11-19.33±1.15. Based on the interaction, the results showed that there was a significant interaction (p>0.05) between the use of Wrf bioactivator with the ratio of CD to RS on the parameters of pH, N-organic and C/ Nratio, while, C-organic and temperature had no significant effect. The results of the study was concluded that several test parameters produced compost characteristics that were in accordance with Standar Nasional Indonesia (SNI) and the Regulation of the Minister of Agriculture of the Republic of Indonesia. Compost production using T3 treatment ((CD:RS)(75%:25%) + 5% of Wrf) produces the best characteristics compared to other treatments.

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