Nanobodies as binding-chaperones stabilize the recombinant Bombyx mori acetylcholinesterase and protect the enzyme activity in pesticide detection

2022 ◽  
pp. 109992
Author(s):  
Jun Cai ◽  
Ema Romão ◽  
Guangpei Wu ◽  
Jiadong Li ◽  
Liping Li ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Bombyx Mori ◽  
Pesticide Detection

scholarly journals Genome organization of the densovirus from Bombyx mori (BmDNV-1) and enzyme activity of its capsid

2001 ◽  
Vol 82 (11) ◽  
pp. 2821-2825 ◽  
Author(s):  
Y. Li ◽  
Z. Zádori ◽  
H. Bando ◽  
R. Dubuc ◽  
G. Fédière ◽  
...  
Keyword(s):  
Amino Acids ◽  
Enzyme Activity ◽  
Bombyx Mori ◽  
Genome Sequence ◽  
Genome Organization ◽  
Phospholipase A ◽  
Sequence Motifs ◽  
Unique Region ◽  
Conserved Sequence

Bombyx mori densovirus (BmDNV-1), on the basis of the previously reported genome sequence, constitutes by itself a separate genus (Iteravirus) within the Densovirinae subfamily of parvoviruses. Inconsistencies in the genome organization, however, necessitated its reassessment. The genome sequence of new clones was determined and resulted in a completely different genome organization. The corrected sequence also contained conserved sequence motifs found in other parvoviruses. Some amino acids in the highly conserved domain in the unique region of VP1 were shared by critical amino acids in the catalytic site and Ca2+-binding loop of secreted phospholipase A2, such as from snake and bee venoms. Expression of this domain and determination of enzyme activity demonstrated that capsids have a phospholipase A2 activity thus far unknown to occur in viruses. This viral phospholipase A2, which is required shortly after entry into the cell, showed a substrate preference for phosphatidylethanolamine and phosphatidylcholine over phosphatidylinositol.

Morphological and biochemical analyses of changes in rat hepatocytes related to wine and ethanol consumption

1984 ◽  
Vol 42 ◽  
pp. 232-233
Author(s):  
S.M. Geyer ◽  
C.L. Mendenhall ◽  
J.T. Hung ◽  
E.L. Cardell ◽  
R.L. Drake ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Enzyme Activity ◽  
Malic Enzyme ◽  
Smooth Endoplasmic Reticulum ◽  
Rat Hepatocytes ◽  
Mature Male ◽  
Treatment Groups ◽  
Malic Enzyme Activity ◽  
Biochemical Analyses

Thirty-three mature male Holtzman rats were randomly placed in 3 treatment groups: Controls (C); Ethanolics (E); and Wine drinkers (W). The animals were fed synthetic diets (Lieber type) with ethanol or wine substituted isocalorically for carbohydrates in the diet of E and W groups, respectively. W received a volume of wine which provided the same gram quantity of alcohol consumed by E. The animals were sacrificed by decapitation after 6 weeks and the livers processed for quantitative triglycerides (T3), proteins, malic enzyme activity (MEA), light microscopy (LM) and electron microscopy (EM). Morphometric analysis of randomly selected LM and EM micrographs was performed to determine organellar changes in centrilobular (CV) and periportal (PV) regions of the liver. This analysis (Table 1) showed that hepatocytes from E were larger than those in C and W groups. Smooth endoplasmic reticulum decreased in E and increased in W compared to C values.

Reductive light activation of enzyme activity

2000 ◽  
Vol 110 (3) ◽  
pp. 295-295
Author(s):  
Louise Anderson ◽  
Per Gardestrom
Keyword(s):  
Enzyme Activity ◽  
Light Activation
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