Epigenetics in the Remission of Anorexia Nervosa: A Follow-up Study of Whole-genome Methylation Profiles

IntroductionAnorexia nervosa (AN) is a severe psychiatric disorder. The epigenetic regulations are strongly suggested in AN. We and other groups have performed a whole-genome methylation study (methylome) in AN. We found that the differentially methylated CpG sites are located around genes involved in biological processes in link with embryonic morphogenesis, brain development and its plasticity, in particular adhesion and axon guidance. Here, we study an independent group of 40 AN patients. Furthermore, we have done a follow-up during more than one year, to compare the methylation profiles in subjects that evolve to the remission.ObjectivesOur work is to replicate the methylome study in an independent AN cohort and to characterize profiles of methylation at two times for the same subjects to compare the AN patients that convert to remitters.AimsOur goal is to identify diagnostic and prognostic epigenetic signatures for AN.MethodsOf the 40 AN patients, 18 evolved to remission. Furthermore, the blood samples of the subjects from the 2 times will be investigated, like this, each subject is its own control. Methylation of DNA is measured by using the Infinium HumanMethylation450 BeadChip technology.ResultsComparisons of AN to controls showed similar profiles of methylation involving the same biological processes as previously identified. We are comparing now the difference of methylation between the 18 remitters and the 18 actual AN, taking into account of the two times of samples.ConclusionsWe expect to characterize specific methylation signature of the prognostic of the AN remission.Disclosure of interestThe authors have not supplied their declaration of competing interest.

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Combined hormonal contraception and risk of venous thromboembolism within the first year following pregnancy

Thrombosis and Haemostasis ◽

10.1160/th13-09-0797 ◽

2014 ◽

Vol 112 (07) ◽

pp. 73-78 ◽

Cited By ~ 1

Author(s):

Thomas Bergholt ◽

Anne Nielsen ◽

Michael J. Paidas ◽

Ellen Christine L. Løkkegaard ◽

Jesper Petersen

Keyword(s):

Venous Thromboembolism ◽

Hormonal Contraception ◽

Hormonal Contraceptives ◽

First Year ◽

The Difference ◽

Combined Hormonal Contraception ◽

One Year ◽

First Time ◽

Combined Hormonal Contraceptives

SummaryEstimating the risk of venous thromboembolism (VTE) associated with combined hormonal contraceptives following early terminated pregnancies or birth, a Danish nationwide retrospective cohort observing a one-year follow-up was defined using three unique registries. All Danish women with confirmed pregnancies aged 15–49 during the period of 1995–2009 were included. The main outcomes were relative and absolute risks of first time venous thromboembolism in users as well as non-users of combined hormonal contraceptives. In 985,569 person-years, 598 venous thromboembolisms were recorded. After early terminated pregnancies and births, respectively, 113 and 485 events occurred in 212,552 and 773,017 person-years. After early terminated pregnancies, the crude VTE incidence ratios were similar, and the numbers needed to harm were equal between groups that did or did not use combined hormonal contraceptives throughout the follow-up year. After childbirth, individuals that used combined hormonal contraceptives were more likely than non-users to experience VTE depicted by crude incidence ratios; however, the difference was only significant after 14 weeks. This implied that the numbers needed to harm were lower for those that used compared to those that did not use combined oral contraceptives in the initial 14 weeks postpartum. In conclusion, the use of combined hormonal contraceptives after early terminated pregnancies was not detrimental, but during the puerperal period, they should be used with caution.

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Impulsivity and Eating Disorders

The British Journal of Psychiatry ◽

10.1192/bjp.162.2.193 ◽

1993 ◽

Vol 162 (2) ◽

pp. 193-197 ◽

Cited By ~ 108

Author(s):

Thomas Fahy ◽

Ivan Eisler

Keyword(s):

Eating Disorders ◽

Anorexia Nervosa ◽

Treatment Trial ◽

Eating Disturbance ◽

Distinct Subgroup ◽

Impulsive Behaviour ◽

Poor Treatment ◽

One Year ◽

Poor Treatment Response

Sixty-seven patients with bulimia nervosa and 29 patients with anorexia nervosa completed the Impulsiveness Questionnaire and questionnaires detailing severity of eating disorder. Bulimic patients had higher impulsivity scores than anorexic patients. Bulimics with high impulsivity scores did not have more severe eating disorders than low scorers. When 39 bulimics and 25 anorexics were interviewed about other impulsive behaviour, 51 % of bulimics and 28% of anorexics reported at least one other impulsive behaviour. Patients with so-called ‘multiimpulsive’ bulimia reported more severe eating disturbance, but this was not reflected on more reliable measures of symptoms. Thirty-nine bulimics entered an eight-week treatment trial and their progress was assessed at eight weeks, 16 weeks and one year. ‘Non-impulsive’ bulimics had a more rapid response than ‘impulsives' during treatment, but there was no difference at follow-up. There was no evidence of an association between high impulsivity trait scores and poor treatment response. It is concluded that impulsivity may shape the expression of eating disorders, but that ‘multi-impulsives' do not constitute a categorically distinct subgroup of bulimics.

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The Effectiveness of Cognitive Behavioral Therapy on Changing Eating Disorder Symptoms and Psychopathology of 32 Anorexia Nervosa Patients at Hospital Discharge and One Year Follow-Up

Annals of Clinical Psychiatry ◽

10.1080/10401230802017068 ◽

2008 ◽

Vol 20 (2) ◽

pp. 79-86 ◽

Cited By ~ 8

Author(s):

Wayne A. Bowers ◽

Lynn S. Ansher

Keyword(s):

Anorexia Nervosa ◽

Eating Disorder ◽

Cognitive Behavioral Therapy ◽

Hospital Discharge ◽

Behavioral Therapy ◽

Cognitive Behavioral ◽

Eating Disorder Symptoms ◽

One Year

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Identifying Effectors of Outcomes in Patients with Large Umbilical Hernias

The American Surgeon ◽

10.1177/000313481608200727 ◽

2016 ◽

Vol 82 (7) ◽

pp. 613-621 ◽

Cited By ~ 3

Author(s):

Steven A. Groene ◽

Davis W. Heniford ◽

Tanushree Prasad ◽

Amy E. Lincourt ◽

Vedra A. Augenstein

Keyword(s):

Umbilical Hernia ◽

Defect Size ◽

Long Term Outcomes ◽

Preoperative Pain ◽

The Mean ◽

The Difference ◽

One Year

Quality of life (QOL) has become an important focus of hernia repair outcomes. This study aims to identify factors which lead to ideal outcomes (asymptomatic and without recurrence) in large umbilical hernias (defect size ≥9 cm2). Review of the prospective International Hernia Mesh Registry was performed. The Carolinas Comfort Scale was used to measure QOL at 1-, 6-, and 12-month follow-up. Demographics, operative details, complications, and QOL data were evaluated using standard statistical methods. Forty-four large umbilical hernia repairs were analyzed. Demographics included: average age 53.6 ± 12.0 and body mass index 34.9 ± 7.2 kg/m2. The mean defect size was 21.7 ± 16.9 cm2, and 72.7 per cent were performed laparoscopically. Complications included hematoma (2.3%), seroma (12.6%), and recurrence (9.1%). Follow-up and ideal outcomes were one month = 28.2 per cent, six months = 42.9 per cent, one year = 55.6 per cent. All patients who remained symptomatic at one and two years were significantly symptomatic before surgery. Symptomatic preoperative activity limitation was a significant predictor of nonideal outcomes at one year ( P = 0.02). Symptomatic preoperative pain was associated with nonideal outcomes at one year, though the difference was not statistically significant ( P = 0.06). Operative technique, mesh choice, and fixation technique did not impact recurrence or QOL. Repair of umbilical hernia with defects ≥9 cm2 had a surprising low rate of ideal outcomes (asymptomatic and no recurrence). All patients with nonideal long-term outcomes had preoperative pain and activity limitations. These data may suggest that umbilical hernia should be repaired when they are small and asymptomatic.

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FOLLOW-UP STUDY OF WHOLE-GENOME DNA METHYLATION EPIGENETIC PROFILES IN THE REMISSION OF ANOREXIA NERVOSA

European Neuropsychopharmacology ◽

10.1016/j.euroneuro.2017.08.136 ◽

2019 ◽

Vol 29 ◽

pp. S857

Author(s):

Nicolas Ramoz ◽

Sébastien Guillaume ◽

Philippe Courtet ◽

Boris Chaumette ◽

Philip Gorwood

Keyword(s):

Dna Methylation ◽

Anorexia Nervosa ◽

Whole Genome ◽

Follow Up Study

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Spectrophotometric Evaluation of Enamel Color Variation Using Infiltration Resin Treatment of White Spot Lesions at One Year Follow-Up

Dentistry Journal ◽

10.3390/dj8020035 ◽

2020 ◽

Vol 8 (2) ◽

pp. 35 ◽

Cited By ~ 2

Author(s):

Roberto Lo Giudice ◽

Frank Lipari ◽

Francesco Puleio ◽

Angela Alibrandi ◽

Fabrizio Lo Giudice ◽

...

Keyword(s):

Color Change ◽

Statistical Significance ◽

Color Variation ◽

White Spot ◽

White Spot Lesions ◽

Color Changes ◽

The Difference ◽

One Year ◽

The Stability

The aim of this study is to evaluate the color changes and the stability at a 1-year follow-up of white spot lesions (WSLs) treated with an infiltrating technique by using etching and TEGDMA resin. The color of 22 white spot lesions and the sound adjacent enamel (SAE) were assessed with a spectrophotometer at T0 (baseline), T1 (after treatment), and T2 (1 year after). The color change ΔE (WSLs-SAE) at T0 vs. T1 were compared to evaluate the camouflage effect efficiency, and at T1 vs. T2 to assess the stability of outcomes. To evaluate the effect on the treatment outcome of gender, the presence or not of previous orthodontic treatment, WSLs onset more/less than 10 years, the age of the patient, and the ΔE WSL (T0 vs. T1) was analyzed. The difference between ΔE (WSLs-SAE) at T0 and T1 resulted in statistical significance (p < 0.01). No statistical difference was found between ΔE (WSLs-SAE) at T1 vs. T2. The variables considered showed no statistical differences in treatment outcomes. The results of our investigation show that the technique used is immediately effective and the camouflage effect keeps up and steady one year after treatment. Such results do not appear to be influenced by analyzed clinical variables.

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Camouflage effects following resin infiltration of postorthodontic white-spot lesions in vivo: One-year follow-up

The Angle Orthodontist ◽

10.2319/050914-334.1 ◽

2015 ◽

Vol 85 (3) ◽

pp. 374-380 ◽

Cited By ~ 19

Author(s):

Amely Eckstein ◽

Hans-Joachim Helms ◽

Michael Knösel

Keyword(s):

White Spot ◽

White Spot Lesions ◽

Female Ratio ◽

Significance Level ◽

Resin Infiltration ◽

Male Female Ratio ◽

The Difference ◽

One Year

ABSTRACT Objective: To assess camouflage effects by concealment of postorthodontic white-spot lesions (WSLs) to sound adjacent enamel (SAE) achieved over 12 months with resin infiltration (Icon, DMG, Hamburg, Germany). Methods: Twenty subjects (trial teeth nteeth = 111) who had received resin infiltration treatment of noncavitated postorthodontic WSLs were contacted for a 1-year follow-up assessment of CIE-L*a*b* colors (T12). Color and lightness (CIE-L*a*b*) data for WSLs and SAE were compared to baseline data assessed before infiltration (T0) and those assessed after 6 months (T6), using a spectrophotometer. The target parameter was the difference between the summarized color and lightness values (ΔEWSL/SAE). Intergroup (WSL, SAE) and intertime comparisons (T0 vs T6, T12) were performed using paired t-tests at a significance level of α = 5%. Results: Nine subjects (trial teeth nteeth = 49; male/female ratio 5/4; age range 13–19 years) were available at T12. After the highly significant reduction of ΔEWSL/SAE discrepancies between T0 and T6, analysis of 12-month records revealed color and lightness discrepancy of WSL vs SAE that was significantly decreased compared with baseline, indicating an assimilation of WSL color to SAE appearance after infiltration, while an additional reduction of discrepancies between T6 and T12 was not significant. Conclusion: As color and lightness characteristics of the Icon infiltrant as well as the esthetic camouflage effects achieved by WSL infiltration were not altered significantly or clinically relevant after 12 months, the method of resin infiltration can be recommended for an enduring esthetic improvement of postorthodontic WSL. (Angle Orthod. 2015;85:374–380.)

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A case report highlights the neglect problem of pregnancy denial

European Psychiatry ◽

10.1016/j.eurpsy.2017.01.1841 ◽

2017 ◽

Vol 41 (S1) ◽

pp. s901-s901

Author(s):

E. Di Giacomo ◽

M. Calabria ◽

F. Colmegna ◽

B. Pucci ◽

M. Clerici

Keyword(s):

Partner Violence ◽

Home Delivery ◽

Psychiatric Evaluation ◽

Childhood Trauma Questionnaire ◽

Mother And Child ◽

Entire Pregnancy ◽

Competing Interest ◽

One Year ◽

Pregnancy Denial

Pregnancy denial was observed in a patient after her second delivery. Contrary to the first pregnancy, she denied weight gain, body changes and baby movements. She reported using a contraceptive pill throughout the entire pregnancy. After a short home delivery without assistance, she was admitted to obstetrical department and referred for psychiatric evaluation. Mrs. T. accepted psychiatric follow-up appointments and was followed up for 6 months. She was initially assessed using the SCID II Interview, beck anxiety and depression interview, WHOQOL (WHO quality of life), and childhood trauma questionnaire. She denied pregnancy concealment and during the period of assessment and follow-up there was no evidence of intimate partner violence. Her female newborn was healthy without consequences of oestrogen/progesteron absorption (hypoplastic left heart syndrome, gastroschisis, hypospadias or congenital urinary trait anomalies) in one year follow up. The patient was discharged after 6 months of clinical outpatient follow-up. This case stresses and emphasizes the health and risk outcomes for both mother and child linked to an underestimated but serious phenomenon such as the denial of pregnancy.Disclosure of interestThe authors have not supplied their declaration of competing interest.

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An Ex-Press implant versus trabeculectomy in a fibrotic bleb with late failure after previous trabeculectomy

International Journal of Ophthalmology ◽

10.18240/ijo.2021.03.08 ◽

2021 ◽

Vol 14 (3) ◽

pp. 383-387

Author(s):

Faried Wagdy ◽

◽

Hisham Elsorogy ◽

Ahmed Alnagdy ◽

Dina Abd Elfattah ◽

...

Keyword(s):

Postoperative Complications ◽

Open Angle Glaucoma ◽

Implant Surgery ◽

Late Failure ◽

Group I ◽

The Mean ◽

The Difference ◽

One Year ◽

Age Range

AIM: To compare the outcome of an Ex-Press implant and subscleral trabeculectomy (SST) in the management of glaucoma after previous trabeculectomy on a fibrotic bleb. METHODS: This randomized prospective study included 28 eyes from 28 patients (age range: 42-55y) with primary open angle glaucoma (POAG) presented with elevated intraocular pressure (IOP) with fibrotic bleb despite previous SST for more than 4mo. The eyes enrolled in the study were divided into two groups: group I (subjected to Ex-Press implant surgery) and group II [subjected to SST with mitomycin C (MMC)]. The follow-up continued one year after surgery to evaluate IOP, visual acuity (VA), visual field (VF), and postoperative complications. RESULTS: A significant decrease in IOP was found in both groups with a higher reduction in Ex-Press implant surgery with the mean IOP of 14.50 mm Hg (P=0.001), while the SST group recorded the mean IOP of 16.50 mm Hg (P=0.001) after one year. However, the difference between the two groups in terms of the decrease in IOP was insignificant. Fewer postoperative complications were recorded in the Ex-Press implant surgery and more cases requiring further anti-glaucomatous medications were seen in the SST group. Both groups showed stability in terms of VA and VF. CONCLUSION: Ex-Press implant surgery and SST with MMC are two surgical alternatives for controlling IOP in late failure that occurs more than 4mo after previous SST with a fibrotic bleb. However, Ex-Press shunt is a safer surgery with fewer complications.

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