Role of PAX5 fusion proteins in B-cell precursor acute lymphoblastic leukemia

2013 ◽  
Vol 41 (8) ◽  
pp. S46
Author(s):  
Leonie Smeenk ◽  
Barbara Werner ◽  
Anna Azaryan ◽  
Meinrad Busslinger
Blood ◽  
2012 ◽  
Vol 119 (1) ◽  
pp. 227-237 ◽  
Author(s):  
Yunqin Lee ◽  
Manesh Chittezhath ◽  
Valentina André ◽  
Helen Zhao ◽  
Michael Poidinger ◽  
...  

Abstract Myelomonocytic cells play a key role in the progression of many solid tumors. However, very little is known about their contribution to the progression of hematopoietic cancers. We investigated the role of monocytes in the progression of human B-cell precursor acute lymphoblastic leukemia (BCP-ALL). We demonstrated that coculturing human monocytes in vitro with CD19+ BCP-ALL blasts from patients “conditioned” them to an inflammatory phenotype characterized by significant up-regulation of the chemokine, CXCL10. This phenotype was also observable ex vivo in monocytes isolated from BCP-ALL patients, which show elevated CXCL10 production compared with monocytes from healthy donors. Functionally, the “conditioned” monocytes promoted migration and invasive capacity of BCP-ALL cells. Increased invasion was mediated by matrix metalloproteinase 9 expression and activity in the BCP-ALL cells induced by the monocyte-derived CXCL10. However, neither the “conditioned” monocytes nor the CXCL10 produced by these cells had any effect on the proliferation/viability of BCP-ALL cells and angiogenesis. Collectively, our results strongly suggest a protumoral role for human monocytes in BCP-ALL, orchestrated by CXCL10 and its effect on tumor cell migration and invasion. These observations highlight the importance of the CXCL10/CXCR3 chemokine circuit in BCP-ALL progression.


2019 ◽  
Author(s):  
Naoto Sakurai ◽  
Yoshihiro Komada ◽  
Ryo Hanaki ◽  
Mari Morimoto ◽  
Takahiro Ito ◽  
...  

2014 ◽  
Vol 42 (8) ◽  
pp. S60
Author(s):  
Leonie Smeenk ◽  
Barbara Werner ◽  
Elin Axelsson ◽  
Monique den Boer ◽  
Sabine Strehl ◽  
...  

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