Detecting and differentiating Theileria sergenti and Theileria sinensis in cattle and yaks by PCR based on major piroplasm surface protein (MPSP)

2010 ◽  
Vol 126 (4) ◽  
pp. 476-481 ◽  
Author(s):  
Aihong Liu ◽  
Guiquan Guan ◽  
Zhijie Liu ◽  
Junlong Liu ◽  
Neil Leblanc ◽  
...  
Keyword(s):  
Surface Protein ◽  
Major Piroplasm Surface Protein

scholarly journals Pathogenic genotype of major piroplasm surface protein associated with anemia in Theileria orientalis infection in cattle

2017 ◽  
Vol 59 (1) ◽  
Author(s):  
Suhee Kim ◽  
Do-Hyeon Yu ◽  
Jeong-Byoung Chae ◽  
Kyoung-Seong Choi ◽  
Hyeon-Cheol Kim ◽  
...  
Keyword(s):  
Surface Protein ◽  
Theileria Orientalis ◽  
Major Piroplasm Surface Protein

scholarly journals Genetic Diversity of Bovine Hemoprotozoa in South Korea

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 768
Author(s):  
Dongmi Kwak ◽  
Min-Goo Seo
Keyword(s):  
South Korea ◽  
Clinical Symptoms ◽  
Surface Protein ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Theileria Orientalis ◽  
Bovine Theileriosis ◽  
Major Piroplasm Surface Protein ◽  
Theileria Spp ◽  
Epidemiological Information

Tick-borne pathogens cause economically significant diseases in cattle. Theileria spp. are parasitic protozoa and the causative agent of bovine theileriosis. Here we report the distribution and risk factors of bovine Theileria using blood samples taken between 2018 and 2019. Of 737 tested cattle, nine animals (1.2%) were positive for Theileria orientalis infection by 18S rRNA gene amplification. Further analysis of the infected samples using the T. orientalis major piroplasm surface protein (MPSP) gene revealed five different genotypes circulating in the population: Types 1, 2, 3, 7, and N3. To the best of our knowledge, this is the first research to describe the existence of the T. orientalis MPSP genotype N3 in South Korea. Although the prevalence of bovine T. orientalis was low, our study offers data on the geographical distribution and prevalence of bovine Theileria spp. in South Korea. Further studies are warranted to determine the correlation of clinical symptoms with parasite MPSP genotypes. Our data provide epidemiological information to help control bovine theileriosis in South Korea.

An outbreak of oriental theileriosis in dairy cattle imported to Vietnam from Australia

Parasitology ◽  
2016 ◽  
Vol 144 (6) ◽  
pp. 738-746 ◽  
Author(s):  
HAGOS GEBREKIDAN ◽  
LINDA NELSON ◽  
GREG SMITH ◽  
ROBIN B. GASSER ◽  
ABDUL JABBAR
Keyword(s):  
Dairy Cattle ◽  
Phylogenetic Analyses ◽  
Surface Protein ◽  
Theileria Orientalis ◽  
Group 3 ◽  
Cattle Blood ◽  
The Difference ◽  
Group 2 ◽  
Major Piroplasm Surface Protein ◽  
Group 1

SUMMARYThis study reports an outbreak of oriental theileriosis in dairy cattle imported to Vietnam from Australia. Following clinical and pathological diagnoses, a total of 112 cattle blood samples were divided into three groups and tested using multiplexed tandem PCR. Group 1 were from aborted heifers in Vietnam; group 2 were from cattle before shipment from group 1 cattle and group 3 were from the same batch of cattle but transported to Taiwan. Theileria orientalis DNA was detected in 72·3% cattle. The prevalences of T. orientalis in groups 1, 2 and 3 were 77·6, 86·9 and 57·5%, respectively, and the difference in prevalence was significant between groups 1 and 3 (P < 0·0001). The infection intensities of genotypes chitose and ikeda of T. orientalis were higher in groups 1 (57 721 and 33 709, respectively) and 3 (5897 and 61 766, respectively) than those in group 2 (2071 and 6331, respectively). Phylogenetic analyses of the major piroplasm surface protein sequences revealed that genotypes chitose and ikeda determined herein were closely related to those previously reported from Australia. This first report of an outbreak of oriental theileriosis in imported cattle emphasizes improved measures for the export and import of cattle infected with T. orientalis.

Generation of IFN-γ-producing cells that recognize the major piroplasm surface protein in Theileria orientalis-infected bovines

2010 ◽  
Vol 171 (3-4) ◽  
pp. 207-215 ◽  
Author(s):  
Takashi Yamaguchi ◽  
Masahiro Yamanaka ◽  
Sanae Ikehara ◽  
Katsuya Kida ◽  
Noritaka Kuboki ◽  
...  
Keyword(s):  
Surface Protein ◽  
Theileria Orientalis ◽  
Ifn Γ ◽  
Major Piroplasm Surface Protein

A molecular phylogeny of the benignTheileriaparasites based on major piroplasm surface protein (MPSP) gene sequences

Parasitology ◽  
2009 ◽  
Vol 137 (2) ◽  
pp. 241-249 ◽  
Author(s):  
W. JEONG ◽  
S. H. YOON ◽  
D. J. AN ◽  
S.-H. CHO ◽  
K.-K. LEE ◽  
...  
Keyword(s):  
Amino Acid ◽  
Sequence Data ◽  
Surface Protein ◽  
Sister Group ◽  
Amino Acid Sequences ◽  
Gene Sequences ◽  
Group Relationships ◽  
Type 3 ◽  
Major Piroplasm Surface Protein

SUMMARYTo investigate the phylogeny of benignTheileriaparasites, we determined the complete major piroplasm surface protein (MPSP) gene sequences for 6 benign theilerial organisms, including the first from tick. Sequences were analysed alongside published sequences for 39 benignTheileriaparasites, using Bayesian inference and maximum parsimony. All MPSP sequences were 852 nucleotides, except for Gansu, Wuchangbuf, VB01, and VB01; Gansu contained 873 nucleotides, and the other 3 had 855. Deduced amino acid sequences contained 284 residues, except for Gansu (291) and Wuchangbuf, VB01, and VB01 (285 each). Pairwise comparisons showed identities among 45 theilerial MPSP sequences ranging from 70·9 to 99·8% for nucleotide and 71·0 to 100% for amino acid sequences. Our results clearly indicate that all global parasites, excluding Brisbane, were classified into 1 of 8 types; 6 types ofTheileriaexist in Korea. Each type, excluding Type 6, has several type-specific amino acid sequences. The phylogenetic tree derived from the nucleotide sequences showed 2 sister-group relationships, Type 2+Type 7 and Type 3+Brisbane, with a new branching pattern: (Type 6 (Type 8 ((Type 2, Type 7), (Type 1, (Type 4, (Type 5, (Type 3, Brisbane))))))). Our sequence data showed no geographical influence on worldwideTheileriaparasite distribution.

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