scholarly journals Genetic Diversity of Bovine Hemoprotozoa in South Korea

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 768
Author(s):  
Dongmi Kwak ◽  
Min-Goo Seo
Keyword(s):  
South Korea ◽  
Clinical Symptoms ◽  
Surface Protein ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Theileria Orientalis ◽  
Bovine Theileriosis ◽  
Major Piroplasm Surface Protein ◽  
Theileria Spp ◽  
Epidemiological Information

Tick-borne pathogens cause economically significant diseases in cattle. Theileria spp. are parasitic protozoa and the causative agent of bovine theileriosis. Here we report the distribution and risk factors of bovine Theileria using blood samples taken between 2018 and 2019. Of 737 tested cattle, nine animals (1.2%) were positive for Theileria orientalis infection by 18S rRNA gene amplification. Further analysis of the infected samples using the T. orientalis major piroplasm surface protein (MPSP) gene revealed five different genotypes circulating in the population: Types 1, 2, 3, 7, and N3. To the best of our knowledge, this is the first research to describe the existence of the T. orientalis MPSP genotype N3 in South Korea. Although the prevalence of bovine T. orientalis was low, our study offers data on the geographical distribution and prevalence of bovine Theileria spp. in South Korea. Further studies are warranted to determine the correlation of clinical symptoms with parasite MPSP genotypes. Our data provide epidemiological information to help control bovine theileriosis in South Korea.

scholarly journals Pathogenic genotype of major piroplasm surface protein associated with anemia in Theileria orientalis infection in cattle

2017 ◽  
Vol 59 (1) ◽  
Author(s):  
Suhee Kim ◽  
Do-Hyeon Yu ◽  
Jeong-Byoung Chae ◽  
Kyoung-Seong Choi ◽  
Hyeon-Cheol Kim ◽  
...  
Keyword(s):  
Surface Protein ◽  
Theileria Orientalis ◽  
Major Piroplasm Surface Protein

An outbreak of oriental theileriosis in dairy cattle imported to Vietnam from Australia

Parasitology ◽  
2016 ◽  
Vol 144 (6) ◽  
pp. 738-746 ◽  
Author(s):  
HAGOS GEBREKIDAN ◽  
LINDA NELSON ◽  
GREG SMITH ◽  
ROBIN B. GASSER ◽  
ABDUL JABBAR
Keyword(s):  
Dairy Cattle ◽  
Phylogenetic Analyses ◽  
Surface Protein ◽  
Theileria Orientalis ◽  
Group 3 ◽  
Cattle Blood ◽  
The Difference ◽  
Group 2 ◽  
Major Piroplasm Surface Protein ◽  
Group 1

SUMMARYThis study reports an outbreak of oriental theileriosis in dairy cattle imported to Vietnam from Australia. Following clinical and pathological diagnoses, a total of 112 cattle blood samples were divided into three groups and tested using multiplexed tandem PCR. Group 1 were from aborted heifers in Vietnam; group 2 were from cattle before shipment from group 1 cattle and group 3 were from the same batch of cattle but transported to Taiwan. Theileria orientalis DNA was detected in 72·3% cattle. The prevalences of T. orientalis in groups 1, 2 and 3 were 77·6, 86·9 and 57·5%, respectively, and the difference in prevalence was significant between groups 1 and 3 (P < 0·0001). The infection intensities of genotypes chitose and ikeda of T. orientalis were higher in groups 1 (57 721 and 33 709, respectively) and 3 (5897 and 61 766, respectively) than those in group 2 (2071 and 6331, respectively). Phylogenetic analyses of the major piroplasm surface protein sequences revealed that genotypes chitose and ikeda determined herein were closely related to those previously reported from Australia. This first report of an outbreak of oriental theileriosis in imported cattle emphasizes improved measures for the export and import of cattle infected with T. orientalis.

Generation of IFN-γ-producing cells that recognize the major piroplasm surface protein in Theileria orientalis-infected bovines

2010 ◽  
Vol 171 (3-4) ◽  
pp. 207-215 ◽  
Author(s):  
Takashi Yamaguchi ◽  
Masahiro Yamanaka ◽  
Sanae Ikehara ◽  
Katsuya Kida ◽  
Noritaka Kuboki ◽  
...  
Keyword(s):  
Surface Protein ◽  
Theileria Orientalis ◽  
Ifn Γ ◽  
Major Piroplasm Surface Protein

scholarly journals Molecular Assay Proves the Presence of Theileria annulata Infection in Camels in Al-Diwaniyah Province, Iraq

2021 ◽  
Author(s):  
Noaman N. A'aiz ◽  
Hayder N. Ayyez ◽  
Ahmed J. Neamah
Keyword(s):  
Dna Amplification ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Molecular Assay ◽  
Conventional Pcr ◽  
Molecular Investigation ◽  
Causative Agents ◽  
The One ◽  
Specific Species ◽  
Theileria Spp

Background: Theileria camelensis and T. dromedarii are parasitic protozoans reported by several studies as specific species that infect the one-humped camel (Camelus dromedarius). However, other findings casted significant doubts on the true identity of the causative species of theileriosis in camels. Therefore, the present study was conducted to investigate of T. camelensis and T. dromedarii in one humped camels in Iraq during Apr-Oct 2017. Methods: Blood samples for DNA extraction were obtained from 181 slaughtered camels. Molecular investigation was performed following the amplification of 18S rRNA gene by conventional PCR technique. DNA sequencing was then utilized only for the positive samples to confirm the infection with the Theileria species. Results: Nine (4.97%) out of 181 examined samples showed a positive result to infection with Theileria spp., and all these appeared as a T. annulata when subjected to DNA amplification and sequencing techniques. There was a complete absence of any new sequence outside the known species. Conclusion: Most of Theileria infection in camels in the study area is caused by T. annulata and no other causative agents like T. camelensis or T. dromedarii.  

Morphology, morphogenesis, and molecular phylogeny of a new brackish water ciliate, Pseudourostyla cristatoides n. sp., from Songjiho lagoon on the coast of East Sea, South Korea

Zootaxa ◽  
2012 ◽  
Vol 3334 (1) ◽  
pp. 42 ◽  
Author(s):  
JAE-HO JUNG ◽  
KYUNG-MIN PARK ◽  
GI-SIK MIN
Keyword(s):  
South Korea ◽  
Body Length ◽  
Brackish Water ◽  
Body Shape ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Genetic Distances ◽  
Rrna Gene ◽  
Contractile Vacuoles

A new brackish water urostylid ciliate, Pseudourostyla cristatoides n. sp. was collected from Songjiho lagoon on the coast ofEast Sea, South Korea, and investigated based on morphology, morphogenesis, and 18S rRNA gene sequences.Pseudourostyla cristatoides is characterized by the following features: slender to elliptical body shape; colourless to dark greyin colour; size in vivo about 220–265 × 85–125μm; flexible and slightly contractile body with narrowly spaced extrusomes(trichocyst type) throughout the whole cell; 2 contractile vacuoles on left side of cell at about 25% and 75% of body length;84–115 adoral membranelles, 20–30 frontal cirri, 1 buccal cirrus, 2 frontoterminal cirri, 17–25 midventral pairs, 2pretransverse, 6–12 transverse cirri, 5–7 left and 4–5 right marginal rows, and 10–13 dorsal kineties; 30–106 macronuclearnodules and 3–5 micronuclei; brackish habitat (salinity 3–5‰). This new species is very similar to P. cristata, but distinguishedprimarily by contractile vacuoles (2 vs. 1 in number; positioned 25%, 75% of body length vs. ahead of mid-body), dorsalkineties (10–13 vs. 8), hyaline layer underneath pellicle (inconspicuous vs. conspicuous), habitat (brackish vs. freshwater), andparticipation of posterior cirri of rear corona in fronto-ventral-transverse cirral anlagen (yes vs. no). Additionally, intra-/inter-specific pairwise genetic distances of the 18S rRNA gene sequences supported that the Songjiho population is a novel species(intra-specific distances of 0.07–0.12% among three P. cristata populations vs. inter-specific distances of 0.93–1.00% between P. cristatoides and three P. cristata populations).

Bovine theileriosis in Australia: a decade of disease

2018 ◽  
Vol 39 (4) ◽  
pp. 215 ◽  
Author(s):  
Cheryl Jenkins
Keyword(s):  
East Coast ◽  
Clinical Disease ◽  
Diverse Range ◽  
Theileria Orientalis ◽  
The Past ◽  
Wildlife Species ◽  
Sequencing Studies ◽  
Cattle Herds ◽  
Bovine Theileriosis ◽  
Theileria Spp

Theileriosis refers to the clinical disease caused by organisms from the genus Theileria, tick-borne haemoprotozoans infecting a diverse range of mammalian hosts. In Australia, Theileria spp. have been identified in both domestic and wildlife species but the bovine parasite, Theileria orientalis, has received the most attention due to the emergence and spread of clinical disease over the past 12 years, particularly in cattle herds on the east coast. At an estimated $20million per annum, the burden to cattle production is significant but despite over a decade of disease, there are still no effective chemotherapeutic treatments or vaccines available in Australia. Recent insights from genome sequencing studies reveal species level diversity within T. orientalis, which may help direct efforts at disease control.

scholarly journals Genotypic Analysis of Piroplasms and Associated Pathogens from Ticks Infesting Cattle in Korea

2020 ◽  
Vol 8 (5) ◽  
pp. 728 ◽  
Author(s):  
Min-Goo Seo ◽  
Oh-Deog Kwon ◽  
Dongmi Kwak
Keyword(s):  
Surface Protein ◽  
Molecular Characteristics ◽  
Haemaphysalis Longicornis ◽  
Theileria Orientalis ◽  
Cattle Ticks ◽  
Genotypic Analysis ◽  
Clinical Complexity ◽  
Tropical Areas ◽  
Anaplasma Bovis ◽  
Major Piroplasm Surface Protein

Tick-borne pathogens (TBPs) impose an important limitation to livestock production worldwide, especially in subtropical and tropical areas. Earlier studies in Korea have examined TBPs residing in ticks and animals; however, information on multiple TBPs in ticks infesting cattle is lacking. This study assessed the prevalence of TBPs in ticks parasitizing cattle. A total of 576 ticks, including 340 adults and 236 nymphs, were collected from cattle in Korea between 2014 and 2018. All ticks collected were identified as Haemaphysalis longicornis based on their morphological and molecular characteristics. Among piroplasms and other tick-associated pathogens, seven TBP genes, namely Theileria orientalis (5.0%), Anaplasma bovis (2.3%), Anaplasma capra (4.7%), Anaplasma phagocytophilum-like Anaplasma spp. (APL) clades A (1.9%) and B (0.5%), Ehrlichia canis (1.6%), and Candidatus Rickettsia longicornii (17.5%), were detected. Bartonella spp. and severe fever with thrombocytopenia syndrome virus were not found. To our knowledge, this is the first study to report the presence of the pathogens T. orientalis major piroplasm surface protein genotypes 3 and 7, A. capra, and APL in ticks from Korea. Cattle ticks may be maintenance hosts for many TBPs, and veterinary and medical clinicians should be aware of their high probability of infection and clinical complexity in humans.

scholarly journals Molecular prevalence of Theileria infections in cattle in Yanbian, north-eastern China

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 19
Author(s):  
Lijun Jia ◽  
Shaowei Zhao ◽  
Suzhu Xie ◽  
Hang Li ◽  
Hao Wang ◽  
...  
Keyword(s):  
Eastern China ◽  
Epidemiological Data ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Protozoan Parasites ◽  
Total Prevalence ◽  
North Eastern ◽  
Bovine Theileriosis ◽  
And Control ◽  
Bovine Erythrocytes

Bovine Theileria are tick-borne protozoan parasites that invade bovine erythrocytes and lymphocytes. Three main bovine Theileria species have been identified in China: T. orientalis, T. sinensis, and T. annulata. To examine the prevalence of bovine theileriosis in Yanbian, a total of 584 bovine blood samples were collected from five localities from 2017 to 2019 and analyzed by PCR. Six pairs of oligonucleotide primers directed against the 18S rRNA gene of Theileria spp., Tams-1 gene of T. annulata, MPSP gene of T. orientalis, and T. sinensis, were used to detect these parasites. A sequence analysis of the amplified genes confirmed that the Theileria species were T. orientalis and T. sinensis, without T. annulata. The overall prevalence of Theileria in cattle was 42.81% (250/584). Out of the 584 samples, 159 (27.23%) and 157 (26.88%) were positive for T. sinensis and T. orientalis, respectively, and the mixed infection rate was 11.30% (66/584). The total prevalence of bovine Theileria species in Helong, Hunchun, Longjing, Yanji, and Dunhua was 66.28%, 49.68%, 23.81%, 28.15%, and 0%, respectively. These results provide epidemiological data for the prevention and control of bovine Theileria species in Yanbian, China.

scholarly journals Parasitological and molecular detection of Babesia canis vogeli in dogs of Recife, Pernambuco and evaluation of risk factors associated

2016 ◽  
Vol 37 (1) ◽  
pp. 163 ◽  
Author(s):  
Vanessa Carla Lima da Silva ◽  
Evilda Rodrigues de Lima ◽  
Mirella Bezerra de Melo Colaço Dias ◽  
Fernanda Lúcia Passos Fukahori ◽  
Michelle Suassuna de Azevedo Rego ◽  
...  
Keyword(s):  
Risk Factors ◽  
Clinical Symptoms ◽  
Clinical Care ◽  
Care Center ◽  
18S Rrna Gene ◽  
Blood Parasites ◽  
Detection Methods ◽  
Rrna Gene ◽  
Babesia Canis ◽  
Factors Associated

This work aims to detect the presence of Babesia canis vogeli in dogs from Recife, Pernambuco via molecular and parasitological detection methods, and to assess the risk factors associated with this parasite. A total of 146 dogs (male and female) of varying breeds and ages that presented clinical symptoms of babesiosis were assessed at a clinical care center in the Veterinary School Hospital. Blood was obtained via venopuncture for hemoparasite detection and polymerase chain reaction (PCR). Using a commercial kit, DNA was extracted from blood samples. For the PCR reaction, an approximately 590 base pair long genetic sequence was used to detect the presence of B. canis vogeli. The forward primer, denoted as BAB1 (5’-GTG AAC CTT ATC ACT TAA AGG-3’), was specific for a conserved region on the 18S rRNA gene of Babesia spp., and the antisense primer was denoted as BAB4 (5’-CAA CTC CTC CAC GCA ATC G-3’). PCR results suggested that the percentage of Babesia canis vogeli infection was 4.8%. Through descriptive statistical analysis of the data, we observed that there was higher frequency of parasite infection associated with male dogs above two years of age, with a defined breed, from the countryside, are domiciled, and also suffer from tick infestation. We conclude that regardless of the type of risk factor, babesiosis can be found throughout Recife, Pernambuco, and its prevalence does not vary in most regions of Brazil. Our results indicate that PCR is a sensitive test for the detection of blood parasites, and should be performed as a clinical routine.

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