Effects of pasteurization, microfiltration, and ultraviolet-c treatments on microorganisms and bioactive proteins in bovine skim milk

2021 ◽  
Vol 43 ◽  
pp. 101339
Author(s):  
Zhibin Li ◽  
Dasong Liu ◽  
Shu Xu ◽  
Wenjin Zhang ◽  
Yacine Hemar ◽  
...  
Keyword(s):  
Skim Milk ◽  
Bioactive Proteins ◽  
Ultraviolet C

Retaining bioactive proteins and extending shelf life of skim milk by microfiltration combined with Ultraviolet-C treatment

LWT ◽  
2021 ◽  
Vol 141 ◽  
pp. 110945
Author(s):  
Wenjin Zhang ◽  
Yaowei Liu ◽  
Zhibin Li ◽  
Shu Xu ◽  
Kasper Hettinga ◽  
...  
Keyword(s):  
Shelf Life ◽  
Skim Milk ◽  
Bioactive Proteins ◽  
Ultraviolet C

Cytotoxic Effect of Pseudomonas aurogenosa Protease on Cancer Cells

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Ghanyia J. Shanyoor ◽  
Fatima R. Abdul ◽  
Nehad A. Taher ◽  
Ihsan A. Raheem
Keyword(s):  
Cell Line ◽  
Normal Cell ◽  
Cytotoxic Effect ◽  
Human Cancer ◽  
Skim Milk ◽  
Exchange Chromatography ◽  
Protease Production ◽  
Normal Cell Line ◽  
Protease Enzyme ◽  
Embryonic Fibroblast

About (20) Pseudomonas rogenosa isolate were experienced for their ability of protease production by calculating the diameter of lysis area after developing on skim milk agar medium (qualitatively ) and the results exhibited that only isolate no (5), was higher isolate for protease making of (26mm) of lysis area. Then, the protein concentration also identified by Bradford method and it was found of 0.16 mg/ ml , then purification was done by using an ion- exchange chromatography with DEAE sephadex G- 100 column and the results showed the presence of 1 peak of enzyme with 50 Kd of molecular weight 2 peaks of other proteins . we tried to investigate the invitro Cytotoxic effect of purified enzyme against two human cancer lines, HeP2 (Human larynx epidermed carcinoma ) , RD ( Rabdo- Sarcoma ) , and one normal cell line Ref ( Rat embryonic fibroblast ) . The cancer and normal cells were treated with different concentrations of protease enzyme ranging from ( 0.05, 0.1, 0.2, 0.4,0.8and 0.16 mg/ml) then incubated for additional 48h at 37C0 and the results showed highest toxicity ( 80.28%) of protease enzyme on RD , moderate cytotoxicity (45.52%) on Hep andslight toxicity ( 37.12% ) on normal cell line (Ref) in a concentration (0.8mg/ml).

Effect of the electro-dialysis treatment temperature on the microflora of skim milk permeate

2018 ◽  
pp. 14-15
Author(s):  
Anisimov G.S. ◽  
◽  
Evdokimov I.A. ◽  
Ryabtseva S.A. ◽  
Donskih A.N. ◽  
...  
Keyword(s):  
Skim Milk ◽  
Treatment Temperature ◽  
Dialysis Treatment ◽  
Milk Permeate

Formation and removal of biofilms in skim milk permeate

2019 ◽  
pp. 48-50 ◽  
Author(s):  
O.V. Salova ◽  
◽  
S.A. Ryabtseva ◽  
Y.A. Tabakova ◽  
G.S. Anisimov ◽  
...  
Keyword(s):  
Skim Milk ◽  
Milk Permeate

Uji Kesukaan Es Krim Kefir Labu Kuning

2017 ◽  
Vol 9 (1) ◽  
pp. 16
Author(s):  
Amanda Kania Diandini
Keyword(s):  
Diabetes Mellitus ◽  
Data Analysis ◽  
Experimental Method ◽  
Skim Milk ◽  
Ice Cream ◽  
Artificial Sweetener ◽  
Value Analysis ◽  
Nutrient Value ◽  
Bioactive Content ◽  
Price Analysis

The safe ice cream which is consumed by Diabetes Mellitus sufferers is made by subtituting skim milk, cream and sugar with kefir, pure pumpkin, cornstarch, vegetable oil, and artificial sweetener special gor Diabetes Mellitus. Kefir is known can decrease (blood sugar) because of its bioactive content. The aim of this research is knowing predilection level test to ice cream pumpkin kefir. This research is conducted with experimental method. The data analysis includes predilection test, nutrient value analysis, and price analysis. Ice cream pumpkin kefir that is liked most are from texture side, the cheapest price, and also the highest fiber content exists in balance 578 with the ratio of kefir and pumpkin 50%:50%. Ice cream pumpkin kefir that is liked most from colour side exists in balance 236 with the ratio of kefir and pure pumpkin 70%:30%. Ice cream pumpkin kefir that is liked most from taste side and aroma exists in balance 522 with the ratio between kefir and pure pumpkin 80%:20%.

Constraints in Adoption ofModern Farm Machines by Tribal Farmers in Ramgarh District of Jharkhand

2019 ◽  
Vol 6 (03) ◽  
Author(s):  
PK SUNDARAM ◽  
BIKASH SARKAR ◽  
UJJWAL KUMAR ◽  
AP ANURAG ◽  
DK RAGHAV ◽  
...  
Keyword(s):  
Cell Line ◽  
Normal Cell ◽  
Human Cancer ◽  
Skim Milk ◽  
Exchange Chromatography ◽  
Protease Production ◽  
Normal Cell Line ◽  
Protease Enzyme ◽  
Tribal Farmers ◽  
Embryonic Fibroblast

About (20) Pseudomonas rogenosa isolate were experienced for their ability of protease production by calculating the diameter of lysis area after developing on skim milk agar medium (qualitatively ) andamp; the results exhibited that only isolate no (5), was higher isolate for protease making of (26mm) of lysis area. Then, the protein concentration also identified by Bradford method andamp; it was found of 0.16 mg/ ml , then purification was done by using an ion- exchange chromatography with DEAE sephadex G- 100 column andamp; the results showed the presence of 1 peak of enzyme with 50 Kd of molecular weight 2 peaks of other proteins . we tried to investigate the invitro Cytotoxic effect of purified enzyme against two human cancer lines, HeP2 (Human larynx epidermed carcinoma ) , RD ( Rabdo- Sarcoma ) , andamp; one normal cell line Ref ( Rat embryonic fibroblast ). The cancer andamp; normal cells were treated with different concentrations of protease enzyme ranging from ( 0.05, 0.1, 0.2, 0.4,0.8andamp; 0.16 mg/ml) then incubated for additional 48h at 37C 0 andamp; the results showed highest toxicity ( 80.28%) of protease enzyme on RD , moderate cytotoxicity (45.52%) on Hep andamp;slight toxicity ( 37.12% )on normal cell line (Ref) in a concentration (0.8mg/ml).

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