Integrated and sustainable separation of chlorogenic acid from blueberry leaves by deep eutectic solvents coupled with aqueous two-phase system

2017 ◽  
Vol 105 ◽  
pp. 205-214 ◽  
Author(s):  
Tong Wang ◽  
Wen-Jing Xu ◽  
Sui-Xin Wang ◽  
Ping Kou ◽  
Peng Wang ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Deep Eutectic Solvents ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Blueberry Leaves

Comparison of hydrophilic and hydrophobic deep eutectic solvents for pretreatment determination of sulfonamides from aqueous environments

2019 ◽  
Vol 11 (46) ◽  
pp. 5901-5909 ◽  
Author(s):  
Yuxin Ma ◽  
Qi Wang ◽  
Tao Zhu
Keyword(s):  
Water Samples ◽  
Extraction Efficiency ◽  
Deep Eutectic Solvents ◽  
Environmental Water ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Aqueous Environments ◽  
Extraction Solvents

Two types of deep eutectic solvents were synthesized for an aqueous two-phase system to determine the sulfonamides from environmental water samples. The ChCl-Ph based ATPS showed good extraction efficiency. The method avoids use of a large number of extraction solvents.

scholarly journals Optimization with genetic algorithms of chlorogenic acid's extraction and purification from native potato (Solanum tuberosum L.) peel, using an aqueous two-phase system

2021 ◽  
Vol 13 (1) ◽  
pp. 377-384
Author(s):  
Doris Inés Paz- Morales ◽  
Carmen Rosa Rojas-Padilla ◽  
Victor Javier Vásquez Villalobos
Keyword(s):  
Genetic Algorithms ◽  
Solanum Tuberosum ◽  
Chlorogenic Acid ◽  
Solanum Tuberosum L ◽  
Dry Weight ◽  
R Package ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Phenolic Components

Chlorogenic acid is one of the main phenolic components of potatoes (Solanum tuberosum L.). Researchers have demonstrated that this phenolic compound is a higher concentration in the native Huagalina potato peel and is also found in the whole tuber's cooking water. This research aims to extract and purify chlorogenic acid (CGA) from potato Huagalina peel obtained from discarded manufacturing for snacks, using an Aqueous Two-Phase System (ATPS). The central composite design rotatable (CDDR); Response Surface Methods (RSM) in R  x 64 4.0.3 and RSM package was used to identify the regions of interest that resulted in the best extraction levels. The concentration of ethanol (EtOH) and disodium phosphate (DSP) were selected as factors capable of affecting CGA performance. Extraction efficiency values for concentrations were optimized using genetic algorithms (GA) applying the R package GA. . In the present research, a high value of 0.8 was applied, which produced new "offspring" solutions, which share some good characteristics taken from both parents. The mutation is applied after the crossover, altering some genes in the chains, which was 0.1. According to what has been reported, the offspring can replace the entire population or replace individuals with less aptitude. This evaluation cycle and selection-reproduction cycle were repeated until a satisfactory recovery of CGA (97.2%) estimated with GA at a pH of 3.4 (25ºC) was achieved, yielding  443.7 ± 0.062 mg CGA / 100 g dry weight of potato peels.  

scholarly journals Efficient Purification of R-phycoerythrin from Marine Algae (Porphyra yezoensis) Based on a Deep Eutectic Solvents Aqueous Two-Phase System

Marine Drugs ◽  
2020 ◽  
Vol 18 (12) ◽  
pp. 618
Author(s):  
Yifeng Xu ◽  
Quanfu Wang ◽  
Yanhua Hou
Keyword(s):  
Driving Forces ◽  
Choline Chloride ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Porphyra Yezoensis ◽  
Deep Eutectic Solvents ◽  
Phase System ◽  
Two Phase ◽  
Native Page ◽  
Aqueous Two Phase System

R-phycoerythrin (R-PE), a marine bioactive protein, is abundant in Porphyra yezoensis with high protein content. In this study, R-PE was purified using a deep eutectic solvents aqueous two-phase system (DES-ATPS), combined with ammonium sulphate precipitation, and characterized by certain techniques. Firstly, choline chloride-urea (ChCl-U) was selected as the suitable DES to form ATPS for R-PE extraction. Then, single-factor experiments were conducted: the purity (A565/A280) of R-PE was 3.825, and the yield was 69.99% (w/w) under optimal conditions (adding 0.040 mg R-PE to ChCl-U (0.35 g)/K2HPO4 (0.8 g/mL, 0.5 mL) and extracting for 20 min). The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) results revealed that the purified R-PE contained three main bands. One band was presented after purification in native-PAGE. The UV-vis spectra showed characteristic absorption peaks at 495, 540, and 565 nm. R-PE displayed an emission wavelength at 570 nm when excited at 495 nm. All spectra results illustrated that the structure of R-PE remained unchanged throughout the process, proving the effectiveness of this method. Transmission electron microscope (TEM) showed that aggregation and surrounding phenomena were the driving forces for R-PE extraction. This study could provide a green and simple purification method of R-PE in drug development.

Enhancing the Sensitivity of DNA and Aptamer Probes in the Dextran/PEG Aqueous Two-Phase System

2021 ◽  
Author(s):  
Qiaoshu Chen ◽  
Yanwen Zhang ◽  
Hui Chen ◽  
Jianbo Liu ◽  
Juewen Liu
Keyword(s):  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System
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