scholarly journals Efficient Purification of R-phycoerythrin from Marine Algae (Porphyra yezoensis) Based on a Deep Eutectic Solvents Aqueous Two-Phase System

Marine Drugs ◽  
2020 ◽  
Vol 18 (12) ◽  
pp. 618
Author(s):  
Yifeng Xu ◽  
Quanfu Wang ◽  
Yanhua Hou
Keyword(s):  
Driving Forces ◽  
Choline Chloride ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Porphyra Yezoensis ◽  
Deep Eutectic Solvents ◽  
Phase System ◽  
Two Phase ◽  
Native Page ◽  
Aqueous Two Phase System

R-phycoerythrin (R-PE), a marine bioactive protein, is abundant in Porphyra yezoensis with high protein content. In this study, R-PE was purified using a deep eutectic solvents aqueous two-phase system (DES-ATPS), combined with ammonium sulphate precipitation, and characterized by certain techniques. Firstly, choline chloride-urea (ChCl-U) was selected as the suitable DES to form ATPS for R-PE extraction. Then, single-factor experiments were conducted: the purity (A565/A280) of R-PE was 3.825, and the yield was 69.99% (w/w) under optimal conditions (adding 0.040 mg R-PE to ChCl-U (0.35 g)/K2HPO4 (0.8 g/mL, 0.5 mL) and extracting for 20 min). The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) results revealed that the purified R-PE contained three main bands. One band was presented after purification in native-PAGE. The UV-vis spectra showed characteristic absorption peaks at 495, 540, and 565 nm. R-PE displayed an emission wavelength at 570 nm when excited at 495 nm. All spectra results illustrated that the structure of R-PE remained unchanged throughout the process, proving the effectiveness of this method. Transmission electron microscope (TEM) showed that aggregation and surrounding phenomena were the driving forces for R-PE extraction. This study could provide a green and simple purification method of R-PE in drug development.

scholarly journals Eco-Innovation in Reusing Food By-Products: Separation of Ovalbumin from Salted Egg White Using Aqueous Two-Phase System of PEG 1000/(NH4)2SO4

Polymers ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 238 ◽  
Author(s):  
Bin Jiang ◽  
Jiaxin Na ◽  
Lele Wang ◽  
Dongmei Li ◽  
Chunhong Liu ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Egg White ◽  
Reversed Phase ◽  
Phase System ◽  
Ionization Mass ◽  
Two Phase ◽  
Aqueous Two Phase System

For the purpose of reducing pollution and the rational use of salted egg white, which is a byproduct of the manufacturing process of salted egg yolk, an aqueous two-phase system (ATPS) composed of polyethylene glycols (PEG 1000) and (NH4)2SO4 was investigated to selectively separate ovalbumin (OVA) from salted egg white. With the aim of optimizing the selective separation of OVA using ATPS, a response surface method (RSM) experiment was carried out on the basis of a single-factor experiment. The OVA was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE), reversed-phase high-performance liquid chromatography (RP-HPLC), liquid chromatography-nano electrospray ionization mass spectrometry (Nano LC-ESI-MS/MS), and Fourier transform infrared spectroscopy (FT-IR). Under the optimal conditions, the recovery yield of OVA through ATPS (Y) and the purity of OVA (P) could reach 89.25% and 96.28%, respectively. In conclusion, OVA was successfully separated from the salted egg white by PEG/(NH4)2SO4 ATPS.

Contribution of different chemical groups to the driving forces for the partition of phenylmethane dyes in the PEO1500 + MgSO4 + H2O aqueous two-phase system

2020 ◽  
Vol 508 ◽  
pp. 112451
Author(s):  
Elkin Dario C. Castrillon ◽  
Yara L. Coelho ◽  
Álvaro Javier P. Agudelo ◽  
Isabela A. Marques ◽  
Eliara A. Hudson ◽  
...  
Keyword(s):  
Driving Forces ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Chemical Groups

Driving forces for chymosin partitioning on the macromolecule-salt aqueous two phase system

2016 ◽  
Vol 100 ◽  
pp. 361-371 ◽  
Author(s):  
Andrés F. Chamorro Rengifo ◽  
Gabriel M. Dias Ferreira ◽  
Guilherme M. Dias Ferreira ◽  
Maria C. Hespanhol da Silva ◽  
Jaqueline de Paula Rezende ◽  
...  
Keyword(s):  
Driving Forces ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Deep eutectic solvents aqueous two-phase system based ultrasonically assisted extraction of ursolic acid (UA) from Cynomorium songaricum Rupr.

2017 ◽  
Author(s):  
Xifeng Zhang ◽  
Ji Zhang
Keyword(s):  
Ursolic Acid ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Deep Eutectic Solvents ◽  
Selective Extraction ◽  
Phase System ◽  
Green Solvents ◽  
Active Components ◽  
Two Phase ◽  
Solid Ratio

Deep eutectic solvents (DESs) are new green solvents that have attracted the attention of the scientific community mainly due to their unique properties and special characteristics, which are different from those of traditional solvents.A method based on ultrasonically assisted deep eutectic solvent aqueous two-phase systems( UAE-DES-ATPS) was developed for extracting ursolic acid (UA) from Cynomorium songaricum Rupr. Four different types of choline chloride-based DESs were prepared.Choline chloride-glucose (ChCl-Glu) exhibited good selective extraction ability. An optimum DES-ATPS of 36% (w/w) ChCl-Glu and 25% (w/w) K2HPO4 was considered to be a satisfactory system for extracting UA. Response surface methodology (RSM) method was used to optimize the extraction of UA using UAE-DES-ATPS. The optimum ultrasound-assisted conditions were as follows: solvent to solid ratio of 15:1 (g/g), ultrasound power of 470 W, and extraction time of 54 min. Compared with the conventional UAE method, the yields were basically the same, but the presented method had higher purity. The structure of UA did not change between pure UA and UA in the upper phase by UV–vis and FT-IR. This approach using ChCl-based DES-ATPS as a novel extraction system and ultrasound as a source of energy provided better choice for the separation of active components from other natural products.

Comparison of hydrophilic and hydrophobic deep eutectic solvents for pretreatment determination of sulfonamides from aqueous environments

2019 ◽  
Vol 11 (46) ◽  
pp. 5901-5909 ◽  
Author(s):  
Yuxin Ma ◽  
Qi Wang ◽  
Tao Zhu
Keyword(s):  
Water Samples ◽  
Extraction Efficiency ◽  
Deep Eutectic Solvents ◽  
Environmental Water ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Aqueous Environments ◽  
Extraction Solvents

Two types of deep eutectic solvents were synthesized for an aqueous two-phase system to determine the sulfonamides from environmental water samples. The ChCl-Ph based ATPS showed good extraction efficiency. The method avoids use of a large number of extraction solvents.

scholarly journals Deep eutectic solvents aqueous two-phase system based ultrasonically assisted extraction of ursolic acid (UA) from Cynomorium songaricum Rupr.

2017 ◽  
Author(s):  
Xifeng Zhang ◽  
Ji Zhang
Keyword(s):  
Ursolic Acid ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Deep Eutectic Solvents ◽  
Selective Extraction ◽  
Phase System ◽  
Green Solvents ◽  
Active Components ◽  
Two Phase ◽  
Solid Ratio

Deep eutectic solvents (DESs) are new green solvents that have attracted the attention of the scientific community mainly due to their unique properties and special characteristics, which are different from those of traditional solvents.A method based on ultrasonically assisted deep eutectic solvent aqueous two-phase systems( UAE-DES-ATPS) was developed for extracting ursolic acid (UA) from Cynomorium songaricum Rupr. Four different types of choline chloride-based DESs were prepared.Choline chloride-glucose (ChCl-Glu) exhibited good selective extraction ability. An optimum DES-ATPS of 36% (w/w) ChCl-Glu and 25% (w/w) K2HPO4 was considered to be a satisfactory system for extracting UA. Response surface methodology (RSM) method was used to optimize the extraction of UA using UAE-DES-ATPS. The optimum ultrasound-assisted conditions were as follows: solvent to solid ratio of 15:1 (g/g), ultrasound power of 470 W, and extraction time of 54 min. Compared with the conventional UAE method, the yields were basically the same, but the presented method had higher purity. The structure of UA did not change between pure UA and UA in the upper phase by UV–vis and FT-IR. This approach using ChCl-based DES-ATPS as a novel extraction system and ultrasound as a source of energy provided better choice for the separation of active components from other natural products.

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