Genetic diversity (RAPD-PCR) of lactobacilli isolated from ?Almagro? eggplant fermentations from two seasons

2004 ◽  
Vol 238 (1) ◽  
pp. 159-165 ◽  
Author(s):  
S SESENA ◽  
I SANCHEZ ◽  
L PALOP
Keyword(s):  
Genetic Diversity ◽  
Rapd Pcr

ANALYSIS OF GENETIC DIVERSITY OF 26 CASSAVA VARIETIES (Manihot esculenta CRANTZ) WITH DIFFERENT RESPONSES TO WATERLOGGING CONDITIONS BY USING RAPD MARKERS

2021 ◽  
Vol 66 (3) ◽  
pp. 170-179
Author(s):  
Sengsoulichan Dethvongsa ◽  
Vu Nguyen Anh ◽  
Van Tran Khanh
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Tree ◽  
Manihot Esculenta ◽  
Rapd Markers ◽  
Flood Tolerance ◽  
Randomly Amplified Polymorphic Dna ◽  
Manihot Esculenta Crantz ◽  
Rapd Pcr ◽  
Cassava Production ◽  
Cassava Varieties

RAPD (Randomly Amplified Polymorphic DNA) is an indicator for high and stable polymorphism, widely used in the study of the diversity of cassava. In this paper, the results of using 20 polymorphic primers OPK combined with the establishment of the phylogenetic tree to analyze the genetic diversity of 26 cassava varieties with different responses to waterlogging conditions by using the RAPD-PCR technique were presented. The purpose of this experiment was to show the genetic relevance of the studied cassava varieties. The results showed that the flood tolerance of cassava was not related to the polymorphism and branching characteristics of the stem. This information may be use as a basis for selecting flood-tolerant cassava varieties for cassava production, as well as the basis for selecting genetically different parents for breeding.

scholarly journals Genetic Diversity of Spiroplasma citri Strains from Different Regions, Hosts, and Isolation Dates

2008 ◽  
Vol 98 (9) ◽  
pp. 960-968 ◽  
Author(s):  
A. F. S. Mello ◽  
R. K. Yokomi ◽  
U. Melcher ◽  
J. C. Chen ◽  
A. C. Wayadande ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Collection Site ◽  
Spiroplasma Citri ◽  
The Past ◽  
Rapd Pcr ◽  
Membrane Lipoprotein ◽  
Polymerase Chain ◽  
Multiple Strains ◽  
Genetic Signatures ◽  
Random Amplified Polymorphism Dna

Spiroplasma citri, a phloem-limited pathogen, causes citrus stubborn disease (CSD). Losses due to CSD in California orchards have grown over the past decade. To investigate the possibility of introduction or emergence of a new strain, a study of genetic diversity among S. citri strains from various locations was conducted using random amplified polymorphism DNA-polymerase chain reaction (RAPD-PCR) of 35 strains cultured from 1980 to 1993, and of 35 strains cultured from 2005 to 2006. Analysis using 20 primer pairs revealed considerable diversity among strains. However, no unique genetic signatures were associated with recently collected strains compared with those collected 15 to 28 years ago, and no geographically associated pattern was distinguishable. S. citri strains from carrot and daikon radish contain some unique DNA fragments, suggesting some host plant influence. Multiple strains from single trees also showed genetic diversity. Sequencing of five RAPD bands that differed among strains showed that diversity-related gene sequences include virus fragments, and fragments potentially encoding a membrane lipoprotein, a DNA modification enzyme, and a mobilization element. No differences in colony morphology were observed among the strains. The lack of correlation between PCR patterns and isolation date or collection site is inconsistent with the hypothesis that recent infections are due to the introduction or emergence of novel pathogen strains.

Genetic Diversity of the Carrion and Jungle Crows as Evidenced by RAPD–PCR Analysis

2003 ◽  
Vol 39 (11) ◽  
pp. 1281-1291 ◽  
Author(s):  
L. N. Spiridonova ◽  
G. N. Chelomina ◽  
A. P. Kryukov
Keyword(s):  
Genetic Diversity ◽  
Pcr Analysis ◽  
Rapd Pcr

Genetic diversity and species identification ofArgulusparasites collected from major aquaculture regions of India using RAPD-PCR

2011 ◽  
Vol 44 (2) ◽  
pp. 220-230 ◽  
Author(s):  
Pramoda Kumar Sahoo ◽  
Jyotirmaya Mohanty ◽  
Sushil Kumar Garnayak ◽  
Bikash Ranjan Mohanty ◽  
Banya Kar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Species Identification ◽  
Rapd Pcr

Genetic diversity among microsporidian isolates from the silkworm, Bombyx mori, as revealed by randomly amplified polymorphic DNA (RAPD) markers

2011 ◽  
Vol 56 (4) ◽  
Author(s):  
B. Surendra Nath ◽  
W. Hassan ◽  
S. Nageswara Rao ◽  
N. Vijaya Prakash ◽  
S. Gupta ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Type Species ◽  
Rapd Markers ◽  
Randomly Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Random Amplification ◽  
Major Cluster ◽  
Sources Of Infection ◽  
Polymerase Chain

AbstractRandom amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) was carried out to assess the genetic diversity of five new microsporidian isolates viz., NIWB-11bp, NIWB-12n, NIWB-13md, NIWB-14b and NIWB-15mb identified from the silkworms. A type species, NIK-1s_mys was used as control for comparison. Differences in the spore shape, length and width were observed. Of the 30 decamer random primers tested, 22 primers gave repeatable RAPD profiles and yielded a total of 143 fragments, of which 78 were polymorphic (55%). The resulting data was used to derive genetic similarity values for constructing a dendrogram. The neighbour joining method based on Dice coefficients indicate a major cluster comprising NIK-1s_mys, NIWB-11bp and NIWB-12n, whereas NIWB-13md, NIWB-14b and NIWB-15mb appear to be different from each other as well from the major cluster mentioned above which includes the type species (NIK-1s_mys). Based on the reproducibility of RAPD profiles, we are able to identify these microsporidians as different isolates. The RAPD technique may be useful in detecting sources of infection of this economically important domestic insect.

Study on morphology, pathogenicity and genetic diversity ofWilsonomyces carpophilusisolates, the causal agent of shot hole of stone fruit trees based on RAPD-PCR in Iran

2012 ◽  
Vol 45 (17) ◽  
pp. 2076-2086 ◽  
Author(s):  
Abdollah Ahmadpour ◽  
Youbert Ghosta ◽  
Mohammad Javan-Nikkhah ◽  
Keyvan Ghazanfari ◽  
Reza Fatahi
Keyword(s):  
Genetic Diversity ◽  
Fruit Trees ◽  
Causal Agent ◽  
Stone Fruit ◽  
Rapd Pcr

scholarly journals TANAMAN INDIKATOR DAN TEKNIK RAPD-PCR UNTUK PENENTUAN BIOTIPE BEMISIA TABACI GENNADIUS (HEMIPTERA: ALEYRODIDAE)

2011 ◽  
Vol 8 (1) ◽  
pp. 1-7
Author(s):  
Purnama Hidayat ◽  
Noor Aidawati ◽  
Sri Hendrastuti Hidayat ◽  
Dewi Sartiami
Keyword(s):  
Genetic Diversity ◽  
Bemisia Tabaci ◽  
Virus Vector ◽  
Indicator Plant ◽  
Chilli Pepper ◽  
Genetic Types ◽  
Rapd Pcr ◽  
Major Pest ◽  
Biotype B

Indicator Plant and PCR-RAPD for Biotype Determination of Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae).B. tabaci has been known world wide as a major pest and virus vector of horticulture. In Indonesia the presence of B.tabaci was reported since 1980 and its role as virus vector in tomato and chilli pepper has becoming more importantrecently. Genetic diversity of B. tabaci has been well recognized, but very little information available for diversity of B.tabaci in Indonesia. This research was conducted in Bogor, West Java from May 2004 to June 2005. The aim of thisresearch was to initiate basic information regarding genetic diversity of B. tabaci in Indonesia, particularly in Java Island.Whiteflies population collected from different crops, i.e. tomato, broccoli, chill pepper, eggplant, cucumber, soybean, andedamame, was evaluated using silverleaf-induction test, and RAPD-PCR. It was evidenced that only B. tabaci populationfrom broccoli was able to induce silverleaf. Two genetic types of B. tabaci, i.e. biotype B and non B, were identified basedon polymorphism character of DNA. Population from broccoli was belong to biotype B, whereas other populations fromtomato, chill pepper, eggplant, cucumber, soybean, and edamame were belong to biotype non B.

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