scholarly journals Detection of genetic diversity among some species of Anthemis L. (Asteraceae) in Saudi Arabia by using RAPD-PCR analysis

2017 ◽  
Vol 11 (4) ◽  
pp. 92-98
Author(s):  
Hasan M. Qari Sameer
Keyword(s):  
Genetic Diversity ◽  
Saudi Arabia ◽  
Pcr Analysis ◽  
Rapd Pcr

Genetic Diversity of the Carrion and Jungle Crows as Evidenced by RAPD–PCR Analysis

2003 ◽  
Vol 39 (11) ◽  
pp. 1281-1291 ◽  
Author(s):  
L. N. Spiridonova ◽  
G. N. Chelomina ◽  
A. P. Kryukov
Keyword(s):  
Genetic Diversity ◽  
Pcr Analysis ◽  
Rapd Pcr

Study on genetic diversity of Castanopsis phuthoensis Luong in Phu Tho province, Vietnam by RAPD markers

2016 ◽  
Vol 14 (2) ◽  
pp. 237-243
Author(s):  
Nguyễn Văn Thọ ◽  
Lê Thị Mai Linh ◽  
Nguyễn Viễn ◽  
Phạm Quang Tiến
Keyword(s):  
Genetic Diversity ◽  
Tree Species ◽  
Dna Polymorphism ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Pcr Analysis ◽  
Dna Fragments ◽  
Similarity Coefficients ◽  
Rapd Pcr ◽  
Rapd Primer

Castanopsis phuthoensis Luong is an endemic tree species of Phu Tho province, only distributed narrowly in forest rehabilitation in two communes belonging to Doan Hung district with density of this species is very low, only from 3.1 to 11.1 trees per hectare. Diameter distributions of the species of number of trees is characterized by curve style with a peak in 20cm or 24cm diameter classes. As it is difficult to find the seedling in the nature, research on forest structure, relations between tree species and genetic diversity is very necessary to define method of conservation for this species. In this study, we used RAPD markers to study on genetic diversity of Castanopsis phuthoensis Luong. The random amplified polymorphic DNA (RAPD) based on the polymeraze chain reaction (PCR) detects nucleotide sequence polymorphism using a single 10mer of arbitrary nucleotided sequence. The RAPD technology has quickly gained widespread acceptance and application because it providesa tool for genetic analysis that have not previously benefited the use of molecular markers. In this study, ten random primers to analyse the genetic diversity of 15 Castanopsis phuthoensis Luong samples that were collected from Phu Tho province, Vietnam. 9 RAPD primers gave DNA polymorphism and 01 RAPD primer OPA20 gave not DNA polymorphism. In the analysis region 0.25-2 kb, there were 56 DNA fragments amplified and 34 DNA fragments were polymorphic. Genetic similarity coefficients of 15 Castanopsis phuthoensis Luong samples ranged from 0.55- 0.95. The phylogenetic tree of 15 samples are divided into two main groups. As results of RAPD-PCR analysis, these samples were collected from the homologous geographical locations and the genetic diversity of 15 samples is not high. Therefore, it is necessary to conserve the "Castanopsis phuthoensis Luong".

scholarly journals Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Kusdianawati KUSDIANAWATI ◽  
Apon Zaenal MUSTOPA ◽  
Fatimah FATIMAH ◽  
Bugi Ratno Budiarto
Keyword(s):  
Genetic Diversity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pcr Analysis ◽  
Phenotypic Characters ◽  
Lactococcus Garvieae ◽  
Rapd Pcr ◽  
Good Potential ◽  
Genotypic Characteristics ◽  
Rrna Sequences

Abstract. Kusdianawati, Mustopa AZ, Fatimah, Budiarto BR. 2020. Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia. Biodiversitas 21: 3225-3233. LAB from Sumbawa horse milk has good potential antimicrobial and probiotic agents. It is known, the study on LAB diversity based on its phenotypic characters is difficult to be distinguished. However, the development of molecular characterization based on the genotypic characteristic could be done for LAB diversity analysis. The aim of this study is to obtain the genetic diversity of LAB from Sumbawa horse milk collected from Penyaring Village and Lennanguar Village, Sumbawa, West Nusa Tenggara Indonesia. LAB strains were identified based on their genotypic characteristics, including their randomly amplified polymorphic DNA (RAPD) primers profiles and 16S ribosomal RNA (rRNA) sequences. The result of RAPD-PCR analysis showed 5 clusters of dendrograms resulted from GTG5 and LB2 primer amplification. Based on 16 rRNA sequences result, the phylogenetic tree was constructed and revealed 7 species of LAB i.e: SK 1.5, SKP K.3, SKP K.5, SKP K.9/SKP K.7/M.SKP K.3, SKL K.4, M.SKL K.1/ M.SKL K.5, and SKP K.4 belonging to the species of Enterococcus faecium, Weissella confusa, Lactococcus garvieae, Enterococcus thailandicus, Lactobacillus fermentum, Enterococcus faecalis, and Lactococcus petauri. In this study, the bacteria from Enterococcus sp., Lactococcus garvieae, and Lactococcus petauri existed as a novel of bacteria which means they have not been isolated and identified in Sumbawa horse milk compared to the previous findings.

scholarly journals Genetic diversity of the ectomycorrhizal fungus Pisolithus tinctorius based on RAPD-PCR analysis

Mycorrhiza ◽  
1998 ◽  
Vol 7 (5) ◽  
pp. 243-248 ◽  
Author(s):  
D. T. Junghans ◽  
E. A. Gomes ◽  
W. V. Guimarães ◽  
E. G. Barros ◽  
E. F. Araújo
Keyword(s):  
Genetic Diversity ◽  
Ectomycorrhizal Fungus ◽  
Pisolithus Tinctorius ◽  
Pcr Analysis ◽  
Rapd Pcr

scholarly journals Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR

2018 ◽  
Vol 19 (5) ◽  
pp. 1714-1721 ◽  
Author(s):  
APON ZAENAL MUSTOPA ◽  
INES FERDIANA PUSPITASARI ◽  
FATIMAH FATIMAH ◽  
LITA TRIRATNA ◽  
GINA KARTINA
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
Staphylococcus Aureus ◽  
16S Rrna ◽  
Pathogenic Bacteria ◽  
Cow’S Milk ◽  
Pcr Analysis ◽  
Selective Media ◽  
Cow's Milk ◽  
Rapd Pcr

Mustopa AZ, Puspitasari IF, Fatimah, Triratna L, Kartina G. 2018. Genetic diversity of mastitis cow’s milk bacteria based onRAPD-PCR. Biodiversitas 19: 1714-1721. Mastitis in cow is caused by several pathogenic bacteria, including antibiotic-resistantbacteria. Identification of the pathogenic bacteria’s diversity that is contaminating cow’s milk needs to be done. The aim of this researchwas to conduct molecular identification in mastitis cow’s milk bacteria through RAPD-PCR (Random Amplified Polymorphism DNAPolymeraseChain Reaction) analysis. Bacteria from mastitis cow’s milk were enumerated using selective media. Based on the result ofmedia selection, there were 72 isolates of bacteria from mastitis cows in Ciguha, Guranteng, Cikarenceng Village (Pagerageung,Tasikmalaya), and Warnasari Village (Pangalengan, Bandung), West Java, Indonesia. The genomes from these isolates were extractedand then subjected to RAPD-PCR analysis. The results of RAPD-PCR analysis showed 8 clusters of dendrogram which 4 dominantclusters were selected. Identification of 4 dominant clusters, which contained representative strains, using 16s rRNA showed the isolatesBPA-12, MHA-6, L-4, and XLDA-8 were identified as Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, andEnterobacter bugandensis.

ANALYSIS OF GENETIC DIVERSITY OF 26 CASSAVA VARIETIES (Manihot esculenta CRANTZ) WITH DIFFERENT RESPONSES TO WATERLOGGING CONDITIONS BY USING RAPD MARKERS

2021 ◽  
Vol 66 (3) ◽  
pp. 170-179
Author(s):  
Sengsoulichan Dethvongsa ◽  
Vu Nguyen Anh ◽  
Van Tran Khanh
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Tree ◽  
Manihot Esculenta ◽  
Rapd Markers ◽  
Flood Tolerance ◽  
Randomly Amplified Polymorphic Dna ◽  
Manihot Esculenta Crantz ◽  
Rapd Pcr ◽  
Cassava Production ◽  
Cassava Varieties

RAPD (Randomly Amplified Polymorphic DNA) is an indicator for high and stable polymorphism, widely used in the study of the diversity of cassava. In this paper, the results of using 20 polymorphic primers OPK combined with the establishment of the phylogenetic tree to analyze the genetic diversity of 26 cassava varieties with different responses to waterlogging conditions by using the RAPD-PCR technique were presented. The purpose of this experiment was to show the genetic relevance of the studied cassava varieties. The results showed that the flood tolerance of cassava was not related to the polymorphism and branching characteristics of the stem. This information may be use as a basis for selecting flood-tolerant cassava varieties for cassava production, as well as the basis for selecting genetically different parents for breeding.

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