scholarly journals Microfluidic sperm sorting device for selection of functional human sperm for IUI application

2016 ◽  
Vol 105 (2) ◽  
pp. e17-e18 ◽  
Author(s):  
Thiruppathiraja chinnasamy ◽  
Barry Behr ◽  
Utkan Demirci
Keyword(s):  
Human Sperm ◽  
Sperm Sorting ◽  
Selection Of

Quantitative selection of single human sperm with high DNA integrity for intracytoplasmic sperm injection

2021 ◽  
Author(s):  
Zhuoran Zhang ◽  
Changsheng Dai ◽  
Guanqiao Shan ◽  
Xin Chen ◽  
Hang Liu ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Human Sperm ◽  
Dna Integrity ◽  
Selection Of

scholarly journals Selection of Functional Human Sperm with Higher DNA Integrity and Fewer Reactive Oxygen Species

2014 ◽  
Vol 3 (10) ◽  
pp. 1671-1679 ◽  
Author(s):  
Waseem Asghar ◽  
Vanessa Velasco ◽  
James L. Kingsley ◽  
Muhammad S. Shoukat ◽  
Hadi Shafiee ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Human Sperm ◽  
Dna Integrity ◽  
Oxygen Species ◽  
Selection Of

A novel approach for the selection of human sperm using annexin V-binding and flow cytometry

2009 ◽  
Vol 91 (4) ◽  
pp. 1285-1292 ◽  
Author(s):  
Christiaan F. Hoogendijk ◽  
Theunis F. Kruger ◽  
Patric J.D. Bouic ◽  
Ralf R. Henkel
Keyword(s):  
Flow Cytometry ◽  
Annexin V ◽  
Human Sperm ◽  
Novel Approach ◽  
Selection Of

scholarly journals A PMMA-Based Microfluidic Device for Human Sperm Evaluation and Screening on Swimming Capability and Swimming Persistence

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 793
Author(s):  
Yimo Yan ◽  
Haoran Liu ◽  
Boxuan Zhang ◽  
Ran Liu
Keyword(s):  
Human Sperm ◽  
High Viscosity ◽  
Control Group ◽  
High Quality ◽  
Vitro Fertilization ◽  
Step Procedure ◽  
Motion Characteristics ◽  
Selection Of

The selection of high-quality sperm is essential to the success of in vitro fertilization (IVF). As human cervical mucus has a high viscosity, without enough swimming persistence, human sperm clouds cannot arrive at the ampulla to fertilize the egg. In this study, we used swimming capability and motion characteristics that are known to be associated with fertilization ability to evaluate the quality of sperm. Here, a clinically applicable polymethyl methacrylate (PMMA)-based microdevice was designed and fabricated for sperm evaluation and screening for swimming capability and persistence in a viscous environment. In this study, we applied methylcellulose (MC) to mimic the natural properties of mucus in vivo to achieve the selection of motile sperm. Sperm motion was recorded by an inverted microscope. The statistical features were extracted and analyzed. Hundreds of sperm in two treated groups with different concentrations of MC and one control group with human tubal fluid (HTF) media were video recorded. This device can achieve a one-step procedure of high-quality sperm selection and achieve the quantitative evaluation of sperm swimming capability and persistence. Sperm with good swimming capability and persistence may be more suitable for fertilization in a viscous environment. This microdevice and methods could be used to guide the evaluation of sperm motility and screening in the future.

scholarly journals Deep learning-based selection of human sperm with high DNA integrity

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Christopher McCallum ◽  
Jason Riordon ◽  
Yihe Wang ◽  
Tian Kong ◽  
Jae Bem You ◽  
...  
Keyword(s):  
Deep Learning ◽  
Human Sperm ◽  
Dna Integrity ◽  
Selection Of

scholarly journals Viscous Cervical Environment-on-a-Chip for Selecting High-Quality Sperm from Human Semen

Biomedicines ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1439
Author(s):  
Manhee Lee ◽  
Jin Woo Park ◽  
Dongwon Kim ◽  
Hyojeong Kwon ◽  
Min Jeong Cho ◽  
...  
Keyword(s):  
Diffusion Mechanism ◽  
Female Reproductive Tract ◽  
Convection Flow ◽  
Dna Integrity ◽  
Cervical Canal ◽  
High Quality ◽  
Sperm Sorting ◽  
Selection Of

When ejaculated sperm travels through the vagina to the uterus, mucus secreted by the cervical canal generally filters out sperm having low motility and poor morphology. To investigate this selection principle in vivo, we developed a microfluidic sperm-sorting chip with a viscous medium (polyvinylpyrrolidone: PVP) to imitate the biophysical environment mimic system of the human cervical canal. The material property of the PVP solution was tuned to the range of viscosities of cervical mucus using micro-viscometry. The selection of high-quality human sperm was experimentally evaluated in vitro and theoretically analyzed by the convection-diffusion mechanism. The convection flow is shown to be dominant at low viscosity of the medium used in the sperm-sorting chip when seeded with raw semen; hence, the raw semen containing sperm and debris convectively flow together with suppressed relative dispersions. Also, it was observed that the sperm selected via the chip not only had high motilities but also normal morphologies and high DNA integrity. Therefore, the biomimetic sperm-sorting chip with PVP medium is expected to improve male fertility by enabling the selection of high-quality sperm as well as uncovering pathways and regulatory mechanisms involved in sperm transport through the female reproductive tract for egg fertilization.

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