CHROMATIN ACCESSIBILITY, TRANSCRIPTIONAL NETWORKS, AND FATTY ACID (FA) SYNTHESIS DURING ADIPOGENESIS IN SUBCUTANEOUS (SC) ADIPOSE STEM CELLS (ASCS) OF POLYCYSTIC OVARY SYNDROME (PCOS) WOMEN

Metabolic characteristics of polycystic ovary syndrome women and polycystic ovary syndrome-like, prenatally androgenized (PA) female monkeys worsen with age, with altered adipogenesis of sc abdominal adipose potentially contributing to age-related adverse effects on metabolism. This study examines whether adipocyte morphology and gene expression in sc abdominal adipose differ between late reproductive-aged PA female rhesus monkeys compared with age-matched controls (C). Subcutaneous abdominal adipose of both groups was obtained for histological imaging and mRNA determination of zinc finger protein 423 (Zfp423) as a marker of adipose stem cell commitment to preadipocytes, and CCAAT/enhancer binding protein (C/EBP)α/peroxisome proliferator-activated receptor (PPAR)δ as well as C/EBPα/PPARγ as respective markers of early- and late-stage differentiation of preadipocytes to adipocytes. In all females combined, serum testosterone (T) levels positively correlated with fasting serum levels of total free fatty acid (r2 = 0.73, P < .002). PA females had a greater population of small adipocytes vs C (P < .001) in the presence of increased Zfp423 (P < .025 vs C females) and decreased C/EBPα (P < .003, vs C females) mRNA expression. Moreover, Zfp423 mRNA expression positively correlated with circulating total free fatty acid levels during iv glucose tolerance testing (P < .004, r2 = 0.66), whereas C/EBPα mRNA expression negatively correlated with serum T levels (P < .02, r2 = 0.43). Gene expression of PPARδ and PPARγ were comparable between groups (P = .723 and P = .18, respectively). Early-to-mid gestational T excess in female rhesus monkeys impairs adult preadipocyte differentiation to adipocytes in sc abdominal adipose and may constrain the ability of this adipose depot to safely store fat with age.

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Use of Photobiomodulation and Mesenchymal Stem Cells in Polycystic Ovary Syndrome‐Induced Rats

The FASEB Journal ◽

10.1096/fasebj.2019.33.1_supplement.580.3 ◽

2019 ◽

Vol 33 (S1) ◽

Author(s):

Eduardo Donato Alves ◽

Luíz Guilherme Dércore Benevenuto ◽

Rebeca Gall Amin ◽

João Antônio Donadoni ◽

Kamile Bausells Ferrari ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Polycystic Ovary Syndrome ◽

Polycystic Ovary ◽

Ovary Syndrome

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Untargeted Metabolomic Approach to Study the Serum Metabolites in Women with Polycystic Ovary Syndrome

10.21203/rs.3.rs-144365/v1 ◽

2021 ◽

Author(s):

Ying YU ◽

PANLI TAN ◽

ZHENCHAO ZHUANG ◽

ZHEJIONG WANG ◽

LINCHAO ZHU ◽

...

Keyword(s):

Fatty Acid ◽

Polycystic Ovary Syndrome ◽

Fatty Acid Biosynthesis ◽

Polycystic Ovary ◽

Ether Lipid ◽

Value Assessment ◽

Ovary Syndrome ◽

Metabolic Marker ◽

The Difference ◽

Acid Pathway

Abstract Background: Polycystic ovary syndrome (PCOS) is a kind of common endocrine syndrome as well as metabolic disorder, which harms the reproductive system and the overall body metabolism of the patients. This study, we aimed to investigate the difference of serum metabolic profiles of the patients with PCOS compared to the healthy controls.Material and methods: a total of 62 study subjects including 31 PCOS patients and 31 matched healthy female controls were recruited, the clinical characteristics data were recorded at the time of recruitment, the laboratory biochemical data were detected. Then, we utilized metabolomics approach by UPLC-HRMS technology to study the metabolic changes between PCOS and controls.Results: The metabolomics analysis showed that there were 68 were downregulated and 78 were upregulated metabolites in PCOS patients serum compared to the controls. These metabolites mainly belong to Triacylglycerols, Glycerophosphocholines, Acylcarnitines, Diacylglycerols, Peptides, Amino acids, Glycerophosphoethanolamines and fatty acid. Pathway analysis showed that these metabolites were enriched in pathways including Glycerophospholipid metabolism, Fatty acid degradation, Fatty acid biosynthesis, Ether lipid metabolism etc. Diagnosis value assessment by ROC analysis showed that the changed metabolites including Leu-Ala/Ile-Ala, 3-(4-Hydroxyphenyl) propionic acid, Ile-Val/Leu-Val, Gly-Val/Val-Gly, aspartic acid, DG(34:2)_DG(16:0/18:2), DG(34:1)_DG(16:0/18:1), Phe-Trp, DG(36:1)_DG(18:0/18:1), Leu-Leu/Leu-Ile had higher AUC values, indicated significant role in PCOS.Conclusion: The present study characterized the difference of metabolic and related pathway profiles in PCOS patients, this finding hope to provide potential metabolic marker for the prognosis and diagnosis of this disease.

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Effects of Sleeve Gastrectomy on Fecal Gut Microbiota and Short-Chain Fatty Acid Content in a Rat Model of Polycystic Ovary Syndrome

Frontiers in Endocrinology ◽

10.3389/fendo.2021.747888 ◽

2021 ◽

Vol 12 ◽

Author(s):

Wei Lin ◽

Lingying Wen ◽

Junping Wen ◽

Guangda Xiang

Keyword(s):

Sleeve Gastrectomy ◽

Fatty Acid ◽

Polycystic Ovary Syndrome ◽

Gut Microbiota ◽

Rat Model ◽

Short Chain Fatty Acid ◽

Polycystic Ovary ◽

Chain Fatty Acid ◽

Short Chain ◽

Ovary Syndrome

PurposeSleeve gastrectomy (SG) is a surgical intervention for polycystic ovary syndrome (PCOS), especially for patients with obesity. Here, we explored the effects of SG on the gut microbiota of rats with PCOS and investigated the association between the intestinal flora and efficacy of SG in PCOS.MethodsDehydroepiandrosterone (DHEA) injection was administered alone and in combination with a high-fat diet to induce PCOS in rats. SG was performed in rats with PCOS, and the effects of SG on the fecal and gut microbiota and the short-chain fatty acid (SCFA) content were observed. Furthermore, the association among gut microbiota, SCFA content and hyperandrogenism or other hallmarks of PCOS was evaluated.ResultsThe abundance of Firmicutes reduced and that of Bacteroidetes increased in response to SG in the DHEA-induced PCOS rat model. At the genus level, the abundances of Bacteroides and Blautia increased and those of Ruminococcus, Clostridium, and Alistipes reduced distinctly in the PCOS-SG groups. Moreover, the levels of fecal SCFAs, especially butyric acid, reduced after SG. SG significantly ameliorated PCOS-related symptoms such as hyperandrogenism, disrupted ovary function, and impaired glucose tolerance. Bacteroides and Blautia exhibited a negative correlation and Ruminococcus, Clostridium, and Alistipes exhibited a positive correlation with the levels of fecal SCFAs, luteinizing hormone, testosterone, and inflammatory factors.ConclusionsThe amelioration of PCOS-related reproductive and metabolic disorders following SG was associated with the regulation of microbial taxa and SCFA content. Our findings provide a novel perspective on the microbial mechanisms in PCOS after SG.

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