bZIP transcription factor SmJLB1 regulates autophagy-related genes Smatg8 and Smatg4 and is required for fruiting-body development and vegetative growth in Sordaria macrospora

ABSTRACT Mating-type genes in fungi encode regulators of mating and sexual development. Heterothallic ascomycete species require different sets of mating-type genes to control nonself-recognition and mating of compatible partners of different mating types. Homothallic (self-fertile) species also carry mating-type genes in their genome that are essential for sexual development. To analyze the molecular basis of homothallism and the role of mating-type genes during fruiting-body development, we deleted each of the three genes, SmtA-1 (MAT1-1-1), SmtA-2 (MAT1-1-2), and SmtA-3 (MAT1-1-3), contained in the MAT1-1 part of the mating-type locus of the homothallic ascomycete species Sordaria macrospora. Phenotypic analysis of deletion mutants revealed that the PPF domain protein-encoding gene SmtA-2 is essential for sexual reproduction, whereas the α domain protein-encoding genes SmtA-1 and SmtA-3 play no role in fruiting-body development. By means of cross-species microarray analysis using Neurospora crassa oligonucleotide microarrays hybridized with S. macrospora targets and quantitative real-time PCR, we identified genes expressed under the control of SmtA-1 and SmtA-2. Both genes are involved in the regulation of gene expression, including that of pheromone genes.

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Nla18, a Key Regulatory Protein Required for Normal Growth and Development of Myxococcus xanthus

Journal of Bacteriology ◽

10.1128/jb.188.5.1733-1743.2006 ◽

2006 ◽

Vol 188 (5) ◽

pp. 1733-1743 ◽

Cited By ~ 20

Author(s):

Michelle E. Diodati ◽

Faisury Ossa ◽

Nora B. Caberoy ◽

Ivy R. Jose ◽

Wataru Hiraiwa ◽

...

Keyword(s):

Gene Expression ◽

Gene Regulation ◽

Fruiting Body ◽

Vegetative Growth ◽

Growth And Development ◽

Expression Patterns ◽

Myxococcus Xanthus ◽

Body Development ◽

Fruiting Body Development ◽

Mutant Cells

ABSTRACT NtrC-like activators regulate the transcription of a wide variety of adaptive genes in bacteria. Previously, we demonstrated that a mutation in the ntrC-like activator gene nla18 causes defects in fruiting body development in Myxococcus xanthus. In this report, we describe the effect that nla18 inactivation has on gene expression patterns during development and vegetative growth. Gene expression in nla18 mutant cells is altered in the early stages of fruiting body development. Furthermore, nla18 mutant cells are defective for two of the earliest events in development, production of the intracellular starvation signal ppGpp and production of A-signal. Taken together, these results indicate that the developmental program in nla18 mutant cells goes awry very early. Inactivation of nla18 also causes a dramatic decrease in the vegetative growth rate of M. xanthus cells. DNA microarray analysis revealed that the vegetative expression patterns of more than 700 genes are altered in nla18 mutant cells. Genes coding for putative membrane and membrane-associated proteins are among the largest classes of genes whose expression is altered by nla18 inactivation. This result is supported by our findings that the profiles of membrane proteins isolated from vegetative nla18 mutant and wild-type cells are noticeably different. In addition to genes that code for putative membrane proteins, nla18 inactivation affects the expression of many genes that are likely to be important for protein synthesis and gene regulation. Our data are consistent with a model in which Nla18 controls vegetative growth and development by activating the expression of genes involved in gene regulation, translation, and membrane structure.

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The transcription factor FvHmg1 negatively regulates fruiting body development in Winter Mushroom Flammulina velutipes

Gene ◽

10.1016/j.gene.2021.145618 ◽

2021 ◽

pp. 145618

Author(s):

Li Meng ◽

Xiaomeng Lyu ◽

Lele Shi ◽

Qingji Wang ◽

Li Wang ◽

...

Keyword(s):

Transcription Factor ◽

Fruiting Body ◽

Flammulina Velutipes ◽

Body Development ◽

Fruiting Body Development

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The Glyoxysomal Protease LON2 Is Involved in Fruiting-Body Development, Ascosporogenesis and Stress Resistance in Sordaria macrospora

Journal of Fungi ◽

10.3390/jof7020082 ◽

2021 ◽

Vol 7 (2) ◽

pp. 82

Author(s):

Antonia Werner ◽

Kolja Otte ◽

Gertrud Stahlhut ◽

Leon M. Hanke ◽

Stefanie Pöggeler

Keyword(s):

Quality Control ◽

Fruiting Body ◽

Protein Quality ◽

Model Organism ◽

Protein Quality Control ◽

Stress Conditions ◽

Dual Function ◽

Sordaria Macrospora ◽

Body Development ◽

Fruiting Body Development

Microbodies, including peroxisomes, glyoxysomes and Woronin bodies, are ubiquitous dynamic organelles that play important roles in fungal development. The ATP-dependent chaperone and protease family Lon that maintain protein quality control within the organelle significantly regulate the functionality of microbodies. The filamentous ascomycete Sordaria macrospora is a model organism for studying fruiting-body development. The genome of S. macrospora encodes one Lon protease with the C-terminal peroxisomal targeting signal (PTS1) serine-arginine-leucine (SRL) for import into microbodies. Here, we investigated the function of the protease SmLON2 in sexual development and during growth under stress conditions. Localization studies revealed a predominant localization of SmLON2 in glyoxysomes. This localization depends on PTS1, since a variant without the C-terminal SRL motif was localized in the cytoplasm. A ΔSmlon2 mutant displayed a massive production of aerial hyphae, and produced a reduced number of fruiting bodies and ascospores. In addition, the growth of the ΔSmlon2 mutant was completely blocked under mild oxidative stress conditions. Most of the defects could be complemented with both variants of SmLON2, with and without PTS1, suggesting a dual function of SmLON2, not only in microbody, but also in cytosolic protein quality control.

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The pro1+ Gene From Sordaria macrospora Encodes a C6 Zinc Finger Transcription Factor Required for Fruiting Body Development

Genetics ◽

10.1093/genetics/152.1.191 ◽

1999 ◽

Vol 152 (1) ◽

pp. 191-199 ◽

Cited By ~ 2

Author(s):

Sandra Masloff ◽

Stefanie Pöggeler ◽

Ulrich Kück

Keyword(s):

Zinc Finger ◽

Fruiting Body ◽

Molecular Mechanisms ◽

Developmental Process ◽

Open Reading Frame ◽

Cosmid Library ◽

Reading Frame ◽

Sordaria Macrospora ◽

Body Development ◽

Fruiting Body Development

Abstract During sexual morphogenesis, the filamentous ascomycete Sordaria macrospora differentiates into multicellular fruiting bodies called perithecia. Previously it has been shown that this developmental process is under polygenic control. To further understand the molecular mechanisms involved in fruiting body formation, we generated the protoperithecia forming mutant pro1, in which the normal development of protoperithecia into perithecia has been disrupted. We succeeded in isolating a cosmid clone from an indexed cosmid library, which was able to complement the pro1- mutation. Deletion analysis, followed by DNA sequencing, subsequently demonstrated that fertility was restored to the pro1 mutant by an open reading frame encoding a 689-amino-acid polypeptide, which we named PRO1. A region from this polypeptide shares significant homology with the DNA-binding domains found in fungal C6 zinc finger transcription factors, such as the GAL4 protein from yeast. However, other typical regions of C6 zinc finger proteins, such as dimerization elements, are absent in PRO1. The involvement of the pro1+ gene in fruiting body development was further confirmed by trying to complement the mutant phenotype with in vitro mutagenized and truncated versions of the pro1 open reading frame. Southern hybridization experiments also indicated that pro1+ homologues are present in other sexually propagating filamentous ascomycetes.

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