scholarly journals Autophagy genesSmatg8andSmatg4are required for fruiting-body development, vegetative growth and ascospore germination in the filamentous ascomyceteSordaria macrospora

Autophagy ◽  
2013 ◽  
Vol 9 (1) ◽  
pp. 33-49 ◽  
Author(s):  
Oliver Voigt ◽  
Stefanie Pöggeler
Keyword(s):  
Fruiting Body ◽  
Vegetative Growth ◽  
Body Development ◽  
Ascospore Germination ◽  
Fruiting Body Development

scholarly journals Nla18, a Key Regulatory Protein Required for Normal Growth and Development of Myxococcus xanthus

2006 ◽  
Vol 188 (5) ◽  
pp. 1733-1743 ◽  
Author(s):  
Michelle E. Diodati ◽  
Faisury Ossa ◽  
Nora B. Caberoy ◽  
Ivy R. Jose ◽  
Wataru Hiraiwa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Regulation ◽  
Fruiting Body ◽  
Vegetative Growth ◽  
Growth And Development ◽  
Expression Patterns ◽  
Myxococcus Xanthus ◽  
Body Development ◽  
Fruiting Body Development ◽  
Mutant Cells

ABSTRACT NtrC-like activators regulate the transcription of a wide variety of adaptive genes in bacteria. Previously, we demonstrated that a mutation in the ntrC-like activator gene nla18 causes defects in fruiting body development in Myxococcus xanthus. In this report, we describe the effect that nla18 inactivation has on gene expression patterns during development and vegetative growth. Gene expression in nla18 mutant cells is altered in the early stages of fruiting body development. Furthermore, nla18 mutant cells are defective for two of the earliest events in development, production of the intracellular starvation signal ppGpp and production of A-signal. Taken together, these results indicate that the developmental program in nla18 mutant cells goes awry very early. Inactivation of nla18 also causes a dramatic decrease in the vegetative growth rate of M. xanthus cells. DNA microarray analysis revealed that the vegetative expression patterns of more than 700 genes are altered in nla18 mutant cells. Genes coding for putative membrane and membrane-associated proteins are among the largest classes of genes whose expression is altered by nla18 inactivation. This result is supported by our findings that the profiles of membrane proteins isolated from vegetative nla18 mutant and wild-type cells are noticeably different. In addition to genes that code for putative membrane proteins, nla18 inactivation affects the expression of many genes that are likely to be important for protein synthesis and gene regulation. Our data are consistent with a model in which Nla18 controls vegetative growth and development by activating the expression of genes involved in gene regulation, translation, and membrane structure.

scholarly journals Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0141586 ◽  
Author(s):  
Hajime Muraguchi ◽  
Kiwamu Umezawa ◽  
Mai Niikura ◽  
Makoto Yoshida ◽  
Toshinori Kozaki ◽  
...  
Keyword(s):  
Fruiting Body ◽  
Rna Seq ◽  
Coprinopsis Cinerea ◽  
Body Development ◽  
Fruiting Body Development

scholarly journals Lessons on fruiting body morphogenesis from genomes and transcriptomes of Agaricomycetes

2021 ◽  
Author(s):  
Laszlo G Nagy ◽  
Peter Jan Vonk ◽  
Markus Kunzler ◽  
Csenge Foldi ◽  
Mate Viragh ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fruiting Body ◽  
Expression Patterns ◽  
Schizophyllum Commune ◽  
Gene Families ◽  
Second Phase ◽  
Fruiting Bodies ◽  
Body Development ◽  
Fruiting Body Development

Fruiting bodies of mushroom-forming fungi (Agaricomycetes) are among the most complex structures produced by fungi. Unlike vegetative hyphae, fruiting bodies grow determinately and follow a genetically encoded developmental program that orchestrates tissue differentiation, growth and sexual sporulation. In spite of more than a century of research, our understanding of the molecular details of fruiting body morphogenesis is limited and a general synthesis on the genetics of this complex process is lacking. In this paper, we aim to comprehensively identify conserved genes related to fruiting body morphogenesis and distill novel functional hypotheses for functionally poorly characterized genes. As a result of this analysis, we report 921 conserved developmentally expressed gene families, only a few dozens of which have previously been reported in fruiting body development. Based on literature data, conserved expression patterns and functional annotations, we provide informed hypotheses on the potential role of these gene families in fruiting body development, yielding the most complete description of molecular processes in fruiting body morphogenesis to date. We discuss genes related to the initiation of fruiting, differentiation, growth, cell surface and cell wall, defense, transcriptional regulation as well as signal transduction. Based on these data we derive a general model of fruiting body development, which includes an early, proliferative phase that is mostly concerned with laying out the mushroom body plan (via cell division and differentiation), and a second phase of growth via cell expansion as well as meiotic events and sporulation. Altogether, our discussions cover 1480 genes of Coprinopsis cinerea, and their orthologs in Agaricus bisporus, Cyclocybe aegerita, Armillaria ostoyae, Auriculariopsis ampla, Laccaria bicolor, Lentinula edodes, Lentinus tigrinus, Mycena kentingensis, Phanerochaete chrysosporium, Pleurotus ostreatus, and Schizophyllum commune, providing functional hypotheses for ~10% of genes in the genomes of these species. Although experimental evidence for the role of these genes will need to be established in the future, our data provide a roadmap for guiding functional analyses of fruiting related genes in the Agaricomycetes. We anticipate that the gene compendium presented here, combined with developments in functional genomics approaches will contribute to uncovering the genetic bases of one of the most spectacular multicellular developmental processes in fungi. Key words: functional annotation; comparative genomics; cell wall remodeling; development; fruiting body morphogenesis; mushroom; transcriptome

scholarly journals Discovery of microRNA-like RNAs during early fruiting body development in the model mushroom Coprinopsis cinerea

PLoS ONE ◽  
2018 ◽  
Vol 13 (9) ◽  
pp. e0198234 ◽  
Author(s):  
Amy Yuet Ting Lau ◽  
Xuanjin Cheng ◽  
Chi Keung Cheng ◽  
Wenyan Nong ◽  
Man Kit Cheung ◽  
...  
Keyword(s):  
Fruiting Body ◽  
Coprinopsis Cinerea ◽  
Body Development ◽  
Fruiting Body Development

scholarly journals Global Gene Expression and Focused Knockout Analysis Reveals Genes Associated with Fungal Fruiting Body Development in Neurospora crassa

2013 ◽  
Vol 13 (1) ◽  
pp. 154-169 ◽  
Author(s):  
Zheng Wang ◽  
Francesc Lopez-Giraldez ◽  
Nina Lehr ◽  
Marta Farré ◽  
Ralph Common ◽  
...  
Keyword(s):  
Gene Expression ◽  
Neurospora Crassa ◽  
Sexual Development ◽  
Fruiting Body ◽  
Rna Silencing ◽  
Global Gene Expression ◽  
Gene Encoding ◽  
Content Type ◽  
Body Development ◽  
Fruiting Body Development

ABSTRACTFungi can serve as highly tractable models for understanding genetic basis of sexual development in multicellular organisms. Applying a reverse-genetic approach to advance such a model, we used random and multitargeted primers to assay gene expression across perithecial development inNeurospora crassa. We found that functionally unclassified proteins accounted for most upregulated genes, whereas downregulated genes were enriched for diverse functions. Moreover, genes associated with developmental traits exhibited stage-specific peaks of expression. Expression increased significantly across sexual development for mating type genemat a-1and format A-1specific pheromone precursorccg-4. In addition, expression of a gene encoding a protein similar to zinc finger,stc1, was highly upregulated early in perithecial development, and a strain with a knockout of this gene exhibited arrest at the same developmental stage. A similar expression pattern was observed for genes in RNA silencing and signaling pathways, and strains with knockouts of these genes were also arrested at stages of perithecial development that paralleled their peak in expression. The observed stage specificity allowed us to correlate expression upregulation and developmental progression and to identify regulators of sexual development. Bayesian networks inferred from our expression data revealed previously known and new putative interactions between RNA silencing genes and pathways. Overall, our analysis provides a fine-scale transcriptomic landscape and novel inferences regarding the control of the multistage development process of sexual crossing and fruiting body development inN. crassa.

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