Isolation and characterization of secondary metabolites from the leaves of Sauropus spatulifolius Beille and their potential biological assays

Fitoterapia ◽  
2021 ◽  
pp. 105100
Author(s):  
Meng Li ◽  
Yadong Cui ◽  
Mei Zhu ◽  
Xiaolei Huang ◽  
Faiz Ur Rehman ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Biological Assays ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Strain Exiguobacterium sp. KRL4, a Producer of Bioactive Secondary Metabolites from a Tibetan Glacier

2021 ◽  
Vol 9 (5) ◽  
pp. 890
Author(s):  
Pietro Tedesco ◽  
Fortunato Palma Esposito ◽  
Antonio Masino ◽  
Giovanni Andrea Vitale ◽  
Emiliana Tortorella ◽  
...  
Keyword(s):  
Phenotypic Analysis ◽  
Biological Assays ◽  
C Elegans ◽  
Isolation And Characterization ◽  
Wide Range ◽  
Protein Encoding ◽  
Nematocidal Activity ◽  
Extremophilic Microorganisms ◽  
Unique Source

Extremophilic microorganisms represent a unique source of novel natural products. Among them, cold adapted bacteria and particularly alpine microorganisms are still underexplored. Here, we describe the isolation and characterization of a novel Gram-positive, aerobic rod-shaped alpine bacterium (KRL4), isolated from sediments from the Karuola glacier in Tibet, China. Complete phenotypic analysis was performed revealing the great adaptability of the strain to a wide range of temperatures (5–40 °C), pHs (5.5–8.5), and salinities (0–15% w/v NaCl). Genome sequencing identified KRL4 as a member of the placeholder genus Exiguobacterium_A and annotation revealed that only half of the protein-encoding genes (1522 of 3079) could be assigned a putative function. An analysis of the secondary metabolite clusters revealed the presence of two uncharacterized phytoene synthase containing pathways and a novel siderophore pathway. Biological assays confirmed that the strain produces molecules with antioxidant and siderophore activities. Furthermore, intracellular extracts showed nematocidal activity towards C. elegans, suggesting that strain KRL4 is a source of anthelmintic compounds.

scholarly journals Chemical and Biological Investigations of Cerbera odollam Gaertn

2018 ◽  
Vol 16 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Monica Sharfin Rahman ◽  
Adnan Faisal ◽  
Choudhury Mahmood Hasan ◽  
Monira Ahsan ◽  
Mohammad Mehedi Masud
Keyword(s):  
Carbon Tetrachloride ◽  
Secondary Metabolites ◽  
Petroleum Ether ◽  
Chromatographic Separation ◽  
Methanolic Extract ◽  
Scientific Basis ◽  
Separation And Purification ◽  
Isolation And Characterization ◽  
Soluble Fractions

The present article describes the isolation and characterization of secondary metabolites from the leaves of Cerbera odollam as well as bioactivities of the crude methanolic extract and its petroleum ether (PE), carbon tetrachloride (CTC), chloroform (CF) and aqueous (AQ) soluble fractions. In this investigation, successive chromatographic separation and purification yielded β-amyrin (CO-01), lupeol (CO-02), β-sitostenone (CO-03) and triticusterol (CO-04). The study also indicated the antioxidant, cytotoxic, antimicrobial, membrane stabilizing activities and poor thrombolytic activities. Thus, the present study endeavors to provide scientific basis to establish Cerbera odollam as a potential medicinal plant.Dhaka Univ. J. Pharm. Sci. 16(2): 179-186, 2017 (December)

scholarly journals Isolation and characterization of new secondary metabolites from Asphodelus microcarpus

2014 ◽  
Vol 23 (7) ◽  
pp. 3510-3515 ◽  
Author(s):  
Mohammed M. Ghoneim ◽  
Khaled M. Elokely ◽  
Atef A. El-Hela ◽  
Abd Elsalam I. Mohammad ◽  
Melissa Jacob ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Isolation And Characterization ◽  
Asphodelus Microcarpus

Isolation and characterization of culmorin derivatives produced by Fusariumculmorum CMI 14764

1992 ◽  
Vol 70 (5) ◽  
pp. 1308-1316 ◽  
Author(s):  
Gertrude C. Kasitu ◽  
John W. ApSimon ◽  
Barbara A. Blackwell ◽  
David A. Fielder ◽  
Roy Greenhalgh ◽  
...  
Keyword(s):  
Spectral Analysis ◽  
Secondary Metabolites ◽  
Liquid Culture ◽  
Farnesyl Pyrophosphate ◽  
Isolation And Characterization ◽  
Derivatives Of

Liquid culture fermentations of Fusariumculmorum yield a wide variety of secondary metabolites. In addition to known isoprenoids, such as the trichothecenes and modified trichothecenes, four new derivatives of culmorin have been isolated and characterized by spectral analysis. These compounds are 15-hydroxyculmorone (7), and 5-hydroxy- (8), 12-hydroxy- (10), and 15-hydroxyculmorin (9). The structure of culmorone is confirmed. The biosynthesis of culmorin from farnesyl pyrophosphate is substantiated through the use of 13C-acetate and the oxidation of these derivatives is discussed.

Isolation and characterization of secondary metabolites fromPlumeria obtusa

2011 ◽  
Vol 13 (12) ◽  
pp. 1122-1127 ◽  
Author(s):  
Muhammad Saleem ◽  
Nasim Akhtar ◽  
Naheed Riaz ◽  
Muhammad Shaiq Ali ◽  
Abdul Jabbar
Keyword(s):  
Secondary Metabolites ◽  
Isolation And Characterization

scholarly journals Preliminary phytochemical profile of Dictyota dichotoma (Huds.) Lamouroux collected from Koothankuzhi Coast, Tirunelveli district, Tamil Nadu, India

2018 ◽  
Vol 8 (6-s) ◽  
pp. 53-56
Author(s):  
Paul J John Peter
Keyword(s):  
Secondary Metabolites ◽  
Tamil Nadu ◽  
Cardiac Glycosides ◽  
East Coast ◽  
Phytochemical Analysis ◽  
Dictyota Dichotoma ◽  
Seaweed Extracts ◽  
Isolation And Characterization ◽  
Phytochemical Profile

The present study was intended to discover the preliminary phytochemicals of Dictyota dichotoma (Huds.) Lamouroux from Koothankuzhi coast, Tirunelveli district, the south east coast of Tamil Nadu, India. The preliminary phytochemical analysis was conducted in seven extracts namely methanol, acetone, chloroform, ethyl acetate, petroleum ether, hexane and benzene by Harborne method. The preliminary phytochemical analysis showed the presence of alkaloids, anthocyanin, anthraquinones, cardiac glycosides, catechin, coumarins, diterpenes, emodins, flavonoids, glycosides, leucoanthocyanin, lignins, phenols, phlobatannins, quinones, saponins, steroids, tannins, terpenoids and triterpenoids. Among the various phytochemicals studied, tannin showed the maximum presence, being found in seven different extracts and anthraquinones was observed in only one extract. From the results, it was noted that the extracts of Dictyota dichotoma (Huds.) Lamouroux was found to be the presence of a number of active secondary metabolites. This report will lead to the isolation and characterization of these active secondary metabolites for bioefficacy and bioactivity. Keywords: Phytochemical, Bioactive compounds, Seaweed extracts, Dictyota, Tamil Nadu.

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