The effects of stainless steel finish on Salmonella Typhimurium attachment, biofilm formation and sensitivity to chlorine

2013 ◽  
Vol 35 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Dov B. Schlisselberg ◽  
Sima Yaron
Keyword(s):  
Stainless Steel ◽  
Salmonella Typhimurium ◽  
Biofilm Formation

Biofilm Development and Sanitizer Inactivation of Listeria monocytogenes and Salmonella typhimurium on Stainless Steel and Buna-n Rubber

1993 ◽  
Vol 56 (9) ◽  
pp. 750-758 ◽  
Author(s):  
AMY B. RONNER ◽  
AMY C. L. WONG
Keyword(s):  
Stainless Steel ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Extracellular Matrices ◽  
Nutrient Level ◽  
Extracellular Materials ◽  
Biofilm Development ◽  
Nutrient Conditions

Biofilm formation by seven strains of Listeria monocytogenes and one strain of Salmonella typhimurium on stainless steel and Buna-n rubber was examined under two nutrient conditions. The type of surface, nutrient level, and organism influenced biofilm development and production of extracellular materials. Buna-n had a strong bacteriostatic effect on L. monocytogenes, and biofilm formation on Buna-n under low nutrient conditions was reduced for four of the seven strains tested. Buna-n was less bacteriostatic toward S. typhimurium. It inhibited the growth of several other pathogens to varying degrees. An ethylene propylene diamine monomer rubber was less inhibitory than Buna-n, and Viton rubber had no effect. The effectiveness of sanitizers on biofilm bacteria was examined. Biofilms were challenged with four types of detergent and nondetergent sanitizers. Resistance to sanitizers was strongly influenced by the type of surface. Bacterial biofilm populations on stainless steel were reduced 3–5 log by all the sanitizers, but those on Buna-n were resistant to these sanitizers and were reduced less than 1–2 log. In contrast, planktonic (suspended) bacteria were reduced 7–8 log by these sanitizers. Chlorine and anionic acid sanitizers generally removed extracellular materials from biofilms better than iodine and quaternary ammonium detergent sanitizers. Scanning electron microscopy demonstrated that biofilm cells and extracellular matrices could remain on sanitized biofilm cells and extracellular matrices could remain surfaces from which no viable cells were recovered.

scholarly journals Biofilm Formation of Salmonella typhimurium on Stainless Steel in Red Meat Model and the Effect of Bacteriophage on Bacterial Biofilm

2018 ◽  
Vol 12 (3) ◽  
pp. 179-188
Author(s):  
Hossein Tajik ◽  
Mehran Moradi ◽  
Mosatafa Alipour ◽  
Hadi Ghasemmahdi ◽  
◽  
...  
Keyword(s):  
Stainless Steel ◽  
Salmonella Typhimurium ◽  
Biofilm Formation ◽  
Red Meat ◽  
Bacterial Biofilm

Inhibition and Inactivation of Salmonella Typhimurium Biofilms from Polystyrene and Stainless Steel Surfaces by Essential Oils and Phenolic Constituent Carvacrol†

2013 ◽  
Vol 76 (2) ◽  
pp. 205-212 ◽  
Author(s):  
KAMLESH A. SONI ◽  
ADEMOLA OLADUNJOYE ◽  
RAMAKRISHNA NANNAPANENI ◽  
M. WES SCHILLING ◽  
JUAN L. SILVA ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Essential Oils ◽  
Salmonella Typhimurium ◽  
Exposure Time ◽  
Crystal Violet ◽  
Biofilm Formation ◽  
Cell Mass ◽  
Minimum Concentration ◽  
Steel Surfaces ◽  
Phenolic Constituent

Persistence of Salmonella biofilms within food processing environments is an important source of Salmonella contamination in the food chain. In this study, essential oils of thyme and oregano and their antimicrobial phenolic constituent carvacrol were evaluated for their ability to inhibit biofilm formation and inactivate preformed Salmonella biofilms. A crystal violet staining assay and CFU measurements were utilized to quantify biofilm cell mass, with evaluating factors such as strain variation, essential oil type, their concentrations, exposure time, as well as biofilm formation surface. Of the three Salmonella strains, Salmonella Typhimurium ATCC 23564 and Salmonella Typhimurium ATCC 19585 produced stronger biofilms than Salmonella Typhimurium ATCC 14028. Biofilm formation by different Salmonella strains was 1.5- to 2-fold higher at 22°C than at 30 or 37°C. The presence of nonbiocidal concentrations of thyme oil, oregano oil, and phenolic carvacrol at 0.006 to 0.012% suppressed Salmonella spp. biofilm formation 2- to 4-fold, but could not completely eliminate biofilm formation. There was high correlation in terms of biofilm inactivation, as determined by the crystal violet–stained optical density (at a 562-nm wavelength) readings and the viable CFU counts. Reduction of biofilm cell mass was dependent on antimicrobial concentration. A minimum concentration of 0.05 to 0.1% of these antimicrobial agents was needed to reduce a 7-log CFU biofilm mass to a nondetectable level on both polystyrene and stainless steel surfaces within 1 h of exposure time.

Effects of Beef Juice on Biofilm Formation by Shiga Toxin–Producing Escherichia coli on Stainless Steel

2020 ◽  
Vol 17 (4) ◽  
pp. 235-242 ◽  
Author(s):  
Zhi Ma ◽  
Kim Stanford ◽  
Xiao M. Bie ◽  
Yan D. Niu ◽  
Tim A. McAllister
Keyword(s):  
Escherichia Coli ◽  
Stainless Steel ◽  
Shiga Toxin ◽  
Biofilm Formation

scholarly journals Innovative Control of Biofilms on Stainless Steel Surfaces Using Electrolyzed Water in the Dairy Industry

Foods ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 103
Author(s):  
Rodrigo Jiménez-Pichardo ◽  
Iriana Hernández-Martínez ◽  
Carlos Regalado-González ◽  
José Santos-Cruz ◽  
Yunny Meas-Vong ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Biofilm Formation ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Dairy Industry ◽  
Raw Milk ◽  
Steel Plates ◽  
Heat Processing ◽  
Rrna Gene ◽  
Electrolyzed Water ◽  
Biofilm Development

Biofilms on food-contact surfaces can lead to recurrent contamination. This work aimed to study the biofilm formation process on stainless steel plates used in the dairy industry: 304 surface finish 2B and electropolished; and the effect of a cleaning and disinfection process using alkaline (AEW) and neutral (NEW) electrolyzed water. Milk fouling during heat processing can lead to type A or B deposits, which were analyzed for composition, surface energy, thickness, and roughness, while the role of raw milk microbiota on biofilm development was investigated. Bacteria, yeasts, and lactic acid bacteria were detected using EUB-338, PF2, and Str-493 probes, respectively, whereas Lis-637 probe detected Listeria sp. The genetic complexity and diversity of biofilms varied according to biofilm maturation day, as evaluated by 16S rRNA gene sequence, denaturing gradient gel electrophoresis, and fluorescence in situ hybridization microscopy. From analysis of the experimental designs, a cleaning stage of 50 mg/L NaOH of AEW at 30 °C for 10 min, followed by disinfection using 50 mg/L total available chlorine of NEW at 20 °C for 5 min is a sustainable alternative process to prevent biofilm formation. Fluorescence microscopy was used to visualize the effectiveness of this process.

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