scholarly journals Determination of carnosine, anserine, homocarnosine, pentosidine and thiobarbituric acid reactive substances contents in meat from different animal species

2011 ◽  
Vol 126 (4) ◽  
pp. 1939-1947 ◽  
Author(s):  
Pier Giorgio Peiretti ◽  
Claudio Medana ◽  
Sonja Visentin ◽  
Valeria Giancotti ◽  
Valentina Zunino ◽  
...  
Keyword(s):  
Animal Species ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances

Distribution of thiobarbituric acid-reactive substances in lipoproteins and proteins in serum.

1989 ◽  
Vol 35 (10) ◽  
pp. 2054-2058 ◽  
Author(s):  
D Bonnefont ◽  
A Legrand ◽  
J Peynet ◽  
J Emerit ◽  
J Delattre ◽  
...  
Keyword(s):  
Apolipoprotein B ◽  
Thiobarbituric Acid ◽  
Precipitation Method ◽  
High Density Lipoproteins ◽  
Separation Procedure ◽  
Serum Lipoproteins ◽  
Thiobarbituric Acid Reactive Substances ◽  
Selective Precipitation ◽  
Acid Reaction

Abstract We assessed the distribution of malondialdehyde (MDA) in lipoproteins and proteins in serum after using two procedures to separate the lipoproteins: sequential ultracentrifugation or selective precipitation with a sodium phosphotungstate and magnesium chloride reagent followed by ultracentrifugation of the supernate. MDA concentrations were determined by the thiobarbituric acid reaction and quantified by fluorometry. We found that 43% of the thiobarbituric acid-reactive substances (TBARS) was bound to the lipoproteins--27% to very-low- and low-density lipoproteins (VLDL-LDL) and 16% to high-density lipoproteins (HDL)--and from 11.5% to 15.8% to proteins, depending on the separation procedure. Residual unbound TBARS were located in the ultracentrifugation layers that contained no lipoproteins or proteins. The TBARS concentration in serum lipoproteins containing apolipoprotein B (i.e., VLDL-LDL) was the same after ultracentrifugation or selective precipitation. We therefore consider the precipitation method more suitable for routine TBARS determination in these lipoproteins, because it is easier to handle and faster. However, for determination of TBARS in HDL, selective precipitation requires subsequent ultracentrifugation at a density of 1.21 kg/L.

Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage

1993 ◽  
Vol 39 (12) ◽  
pp. 2522-2526 ◽  
Author(s):  
W Wasowicz ◽  
J Nève ◽  
A Peretz
Keyword(s):  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Fluorometric Method ◽  
C Storage ◽  
Insulin Dependent ◽  
The Mean ◽  
Optimized Conditions ◽  
And Storage

Abstract A simple, reliable, and reproducible fluorometric method for measuring thiobarbituric acid-reactive substances (TBARS) in serum is proposed, based on the reaction between malondialdehyde (MDA) and thiobarbituric acid. Formation of TBARS was complete at pH 2.4-2.6, but extraction with n-butanol proved complete only at lower pH, i.e., 1.6-1.7. Analytical recoveries of MDA added to serum were 94%-101%; within- and between-run CVs were 2.4-3.6% and 4.6-5.5%; and the detection limit for TBARS in serum was 0.10 mumol/L. Optimized conditions included: (a) collection of either serum or heparinized plasma, (b) preservation from in vitro autoxidation by glutathione and EDTA, and (c) storage at -20 degrees C up to 35 days. The mean (+/- SD) TBARS concentration in 47 healthy adults was 1.01 (0.21) mumol/L; no sex-related difference was observed. Higher concentrations were measured in patients with renal insufficiency undergoing hemodialysis and in patients with insulin-dependent diabetes, chronic pancreatitis, or liver cirrhosis.

Determination of Antioxidant Components and Activity of Tamarix ramosissima Comparative with Vaccinium myrtillus on Streptozotocin-diabetic Mice

2018 ◽  
Vol 69 (7) ◽  
pp. 1860-1865
Author(s):  
Luciana Teodora Rotaru ◽  
Renata Maria Varut ◽  
Mihai Banicioiu Covei ◽  
Irina Iuliana Costache ◽  
Marius Novac ◽  
...  
Keyword(s):  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Vaccinium Myrtillus ◽  
Tamarix Ramosissima ◽  
Diabetic Mice ◽  
Flavonoid Content ◽  
Thiobarbituric Acid Reactive Substances ◽  
Total Polyphenol

Tamarix ramosissima (Tamaricaceae) is a small tree that grows spontaneously in Europe and Asia, being considered an invasive species in geographical areas with warm climates. The chemical composition is partially elucidated, being empirically used for antiinflammatory, analgesic, antibacterial and antioxidant effect. Our study aimed to evaluate the total polyphenol and flavonoid content of vegetal extracts and to test in vivo antioxidant therapeutic effect of it, comparative with Vaccinium myrtillus, using streptozotocin-induced diabetic mice. After five weeks the animals were sacrificed and we determined erythrocyte activities of superoxide dismutase, glutathione peroxidase, glutathione reductase and level of lipid peroxides as thiobarbituric acid reactive substances. Antioxidant enzymes had highest activities in mice treated with T. ramosissima extract and the level of lipid peroxides was the lowest. The tested extract had higher content of polyphenols comparative with V. myrtillus. Our results sustain the efficiency of T. ramosissima extracts on normalizing the effects of oxidative stress in diabetes.

scholarly journals Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Adam Oleszko ◽  
Sylwia Olsztyńska-Janus ◽  
Tomasz Walski ◽  
Karolina Grzeszczuk-Kuć ◽  
Jolanta Bujok ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Plasma Lipid ◽  
Thiobarbituric Acid ◽  
Breakdown Product ◽  
Thiobarbituric Acid Reactive Substances ◽  
Reflectance Ftir ◽  
The Fourier Transform ◽  
Atr Spectroscopy ◽  
Plasma Lipid Peroxidation

During a haemodialysis (HD), because of the contact of blood with the surface of the dialyser, the immune system becomes activated and reactive oxygen species (ROS) are released into plasma. Particularly exposed to the ROS are lipids and proteins contained in plasma, which undergo peroxidation. The main breakdown product of oxidized lipids is the malondialdehyde (MDA). A common method for measuring the concentration of MDA is a thiobarbituric acid reactive substances (TBARS) method. Despite the formation of MDA in plasma during HD, its concentration decreases because it is removed from the blood in the dialyser. Therefore, this research proposes the Fourier Transform Infrared Attenuated Total Reflectance (FTIR-ATR) spectroscopy, which enables determination of primary peroxidation products. We examined the influence of the amount of hydrogen peroxide added to lipid suspension that was earlier extracted from plasma specimen on lipid peroxidation with use of TBARS and FTIR-ATR methods. Linear correlation between these methods was shown. The proposed method was effective during the evaluation of changes in the extent of lipid peroxidation in plasma during a haemodialysis in sheep. A measurement using the FTIR-ATR showed an increase in plasma lipid peroxidation after 15 and 240 minutes of treatment, while the TBARS concentration was respectively lower.

Chronic hypoxia alters glucose utilization during GSH-dependent detoxication in rat small intestine

1998 ◽  
Vol 274 (2) ◽  
pp. G376-G384 ◽  
Author(s):  
Terry S. Legrand ◽  
Tak Yee Aw
Keyword(s):  
Glucose Utilization ◽  
Lipid Peroxide ◽  
Thiobarbituric Acid ◽  
Sprague Dawley ◽  
Thiobarbituric Acid Reactive Substances ◽  
Disulfide Reduction ◽  
Sprague Dawley Rats ◽  
The Impact

We showed that hypoxia alters glutathione (GSH)-dependent detoxication and induces mucosal metabolic instability. To determine the impact of these changes and the role of reductant supply in intestinal lipid peroxide disposition, pair-fed (16 g/day) Sprague-Dawley rats were exposed to air (20.9% O2; n = 6) or 10% O2( n = 6) for 10 days. Jejunal and ileal everted sacs were exposed to 75 μM peroxidized fish oil with or without 10 mM glucose or 1 mM GSH. Peroxide transport was determined as the abluminal recovery of thiobarbituric acid-reactive substances. Peroxide recovery in hypoxic intestine was twice that in normoxic intestine. Addition of GSH and glucose did not affect peroxide recovery, indicating reduced intracellular GSH-dependent metabolism and enhanced output by the hypoxic intestine. Glucose uptake by normoxic and hypoxic intestine is similar, whereas its utilization for detoxication is decreased in hypoxic cells. Determination of NADPH supply indicates that decreased glucose availability for NADPH production during hypoxia impairs GSH disulfide reduction, compromises hydroperoxide metabolism, and increases peroxide output from hypoxic intestine.

scholarly journals The Determination of TBA-Reactive Substances and Alkenals in the Presence of Antioxidants

1999 ◽  
Vol 47 (2) ◽  
pp. 155-159
Author(s):  
S. T. Balthazary ◽  
H.-P. Sallmann ◽  
H. Fuhrmann
Keyword(s):  
Gas Chromatography ◽  
Sample Preparation ◽  
Carbonic Acid ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Effective Antioxidant ◽  
Inhibitory Efficiency ◽  
Liver Homogenates ◽  
Aldehyde Determination

This study investigated the inhibitory efficiency of all-rac-α-tocopherol, 2,6- ditert-butyl-p-cresole (BHT), and 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carbonic acid (Trolox®) on determination of thiobarbituric acid-reactive substances (TBARS) and short-chain alkenals in rat liver homogenates. The concentration of TBARS was measured fluorophotometrically. Aldehydes were determined after derivatization with methylhydrazine by gas chromatography (GLC). The concentrations of alkenals and TBARS in liver homogenates were diminished when antioxidants were present dur- ing the sample preparation. It is suggested that in the absence of antioxidants the samples are autoxidized further during the preparative procedures. For the aldehyde determination all-rac-α-tocopherol was the most effective antioxidant to reduce the bias due to autoxidation, whereas for TBARS it was Trolox.

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