Green separation of bromelain in food sample with high retention of enzyme activity using recyclable aqueous two-phase system containing a new synthesized thermo-responsive copolymer and salt

2019 ◽  
Vol 282 ◽  
pp. 48-57 ◽  
Author(s):  
Lei Wang ◽  
Wenxuan Li ◽  
Yuanyuan Liu ◽  
Wenjing Zhi ◽  
Juan Han ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Food Sample ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Green Separation

scholarly journals Optimization of Aqueous Two-Phase System (ATPS) of Recombinant Bromelain by Response Surface Methodology

2018 ◽  
Vol 7 (4.30) ◽  
pp. 377
Author(s):  
Zatul Iffah Mohd Arshad ◽  
Azura Amid
Keyword(s):  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Partition Coefficient ◽  
Response Surface ◽  
Potassium Phosphate ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Crude Sample ◽  
Face Centered

Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-centered central composite design (FCCCD) model. The optimum conditions for purification were at 18.47% [w/w] PEG6000 and 13% [w/w] potassium phosphate, pH 7.0 with enzyme activity was obtained as 0.272±0.0036 unit m/L, and partition coefficient as 1.394±0.093. The recombinant bromelain was preferentially partitioned into the top phase and the band was reduced in contrast to crude sample on SDS-PAGE gel.

scholarly journals Label-Free Direct Visual Analysis of Hydrolytic Enzyme Activity Using Aqueous Two-Phase System Droplet Phase Transitions

2014 ◽  
Vol 86 (8) ◽  
pp. 4052-4057 ◽  
Author(s):  
David Lai ◽  
John P. Frampton ◽  
Michael Tsuei ◽  
Albert Kao ◽  
Shuichi Takayama
Keyword(s):  
Enzyme Activity ◽  
Phase Transitions ◽  
Visual Analysis ◽  
Hydrolytic Enzyme ◽  
Phase System ◽  
Label Free ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Droplet Phase

Enhancing the Sensitivity of DNA and Aptamer Probes in the Dextran/PEG Aqueous Two-Phase System

2021 ◽  
Author(s):  
Qiaoshu Chen ◽  
Yanwen Zhang ◽  
Hui Chen ◽  
Jianbo Liu ◽  
Juewen Liu
Keyword(s):  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Continuous Purification of an Enveloped and Non-enveloped Viral Particle Using an Aqueous Two-Phase System

2021 ◽  
pp. 118753
Author(s):  
Dylan G. Turpeinen ◽  
Pratik U. Joshi ◽  
Seth A. Kriz ◽  
Supreet Kaur ◽  
Natalie M. Nold ◽  
...  
Keyword(s):  
Viral Particle ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Partitioning of bovine lactoferrin in aqueous two-phase system containing poly(ethylene glycol) and sodium citrate

2015 ◽  
Vol 95 ◽  
pp. 118-124 ◽  
Author(s):  
Angélica Ribeiro da Costa ◽  
Jane Sélia dos Reis Coimbra ◽  
Luísa Alexandre Ferreira ◽  
João Carlos Marcos ◽  
Igor José Boggione Santos ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Sodium Citrate ◽  
Bovine Lactoferrin ◽  
Phase System ◽  
Poly Ethylene Glycol ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Poly Ethylene
Sign in / Sign up

Export Citation Format

Share Document