scholarly journals Label-Free Direct Visual Analysis of Hydrolytic Enzyme Activity Using Aqueous Two-Phase System Droplet Phase Transitions

2014 ◽  
Vol 86 (8) ◽  
pp. 4052-4057 ◽  
Author(s):  
David Lai ◽  
John P. Frampton ◽  
Michael Tsuei ◽  
Albert Kao ◽  
Shuichi Takayama
Keyword(s):  
Enzyme Activity ◽  
Phase Transitions ◽  
Visual Analysis ◽  
Hydrolytic Enzyme ◽  
Phase System ◽  
Label Free ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Droplet Phase

Non-inertial lift induced migration for label-free sorting of cells in a co-flowing aqueous two-phase system

The Analyst ◽  
2019 ◽  
Vol 144 (8) ◽  
pp. 2574-2583 ◽  
Author(s):  
S. Hazra ◽  
K. S. Jayaprakash ◽  
K. Pandian ◽  
A. Raj ◽  
S. K. Mitra ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Mononuclear Cells ◽  
Phase System ◽  
Label Free ◽  
Two Phase ◽  
Peripheral Blood Mononuclear ◽  
Aqueous Two Phase System ◽  
System P ◽  
Blood Mononuclear Cells ◽  
Microfluidic Technique

We present a novel label-free passive microfluidic technique for isolation of cancer cells (EpCAM+ and CD45−) from peripheral blood mononuclear cells (PBMCs) (CD45+ and EpCAM−) in aqueous two-phase system (ATPS).

scholarly journals Optimization of Aqueous Two-Phase System (ATPS) of Recombinant Bromelain by Response Surface Methodology

2018 ◽  
Vol 7 (4.30) ◽  
pp. 377
Author(s):  
Zatul Iffah Mohd Arshad ◽  
Azura Amid
Keyword(s):  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Partition Coefficient ◽  
Response Surface ◽  
Potassium Phosphate ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Crude Sample ◽  
Face Centered

Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-centered central composite design (FCCCD) model. The optimum conditions for purification were at 18.47% [w/w] PEG6000 and 13% [w/w] potassium phosphate, pH 7.0 with enzyme activity was obtained as 0.272±0.0036 unit m/L, and partition coefficient as 1.394±0.093. The recombinant bromelain was preferentially partitioned into the top phase and the band was reduced in contrast to crude sample on SDS-PAGE gel.

Microscopic evaluation of aqueous two-phase system emulsion characteristics enables rapid determination of critical polymer concentrations for solution micropatterning

The Analyst ◽  
2017 ◽  
Vol 142 (11) ◽  
pp. 1938-1945 ◽  
Author(s):  
Mackenzie Ruthven ◽  
Kristin Robin Ko ◽  
Rishima Agarwal ◽  
John P. Frampton
Keyword(s):  
Phase Transitions ◽  
Simple Technique ◽  
Rapid Determination ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Microscopic Evaluation ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

A simple technique is presented to identify phase-transitions in polymeric aqueous two-phase systems.

Enhancing the Sensitivity of DNA and Aptamer Probes in the Dextran/PEG Aqueous Two-Phase System

2021 ◽  
Author(s):  
Qiaoshu Chen ◽  
Yanwen Zhang ◽  
Hui Chen ◽  
Jianbo Liu ◽  
Juewen Liu
Keyword(s):  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Continuous Purification of an Enveloped and Non-enveloped Viral Particle Using an Aqueous Two-Phase System

2021 ◽  
pp. 118753
Author(s):  
Dylan G. Turpeinen ◽  
Pratik U. Joshi ◽  
Seth A. Kriz ◽  
Supreet Kaur ◽  
Natalie M. Nold ◽  
...  
Keyword(s):  
Viral Particle ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Partitioning of bovine lactoferrin in aqueous two-phase system containing poly(ethylene glycol) and sodium citrate

2015 ◽  
Vol 95 ◽  
pp. 118-124 ◽  
Author(s):  
Angélica Ribeiro da Costa ◽  
Jane Sélia dos Reis Coimbra ◽  
Luísa Alexandre Ferreira ◽  
João Carlos Marcos ◽  
Igor José Boggione Santos ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Sodium Citrate ◽  
Bovine Lactoferrin ◽  
Phase System ◽  
Poly Ethylene Glycol ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Poly Ethylene
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