Development and validation of the one-step purification method coupled to LC-MS/MS for simultaneous determination of four aflatoxins in fermented tea

Background: Food and herbal extracts rich in Quercetin (QRT) are often self-medicated by diabetics and can potentially alter the pharmacokinetics (PK) of Metformin HCl (MET) and Canagliflozin (CNG) leading to food or herb-drug interactions and reduced therapeutic efficacy. However, the impact of these flavonoids on the pharmacokinetic behaviour of MET and CNG is mostly unknown. Methods: A simple one-step protein precipitation method was developed for the determination of MET and CNG from rat plasma. The mobile phase chosen was MeOH 65% and 35% water containing 0.1% formic acid at a flow rate of 1mL/min. Results: The retention time of MET, internal standard (Valsartan) and CNG was 1.83, 6.2 and 8.2 min, respectively. The method was found to be linear in the range of 200 - 8000 ng/mL for CNG and 100 = 4000 ng/ml for MET. Precision and accuracy of the method were below 20% at LLOQ and below 15% for LQC, MQC, and HQC. Conclusion: The method was successfully applied for the determination of PK of MET and CNG by using 100 μL of rat plasma. QRT co-administration affects the PK parameters of MET and CNG. This alteration in PK parameters might be of significant use for clinicians and patients.

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Development and validation of a high-performance thin-layer chromatographic method for the simultaneous determination of levamisole and cocaine in seized cocaine sample

JPC - Journal of Planar Chromatography - Modern TLC ◽

10.1556/1006.2018.31.5.6 ◽

2018 ◽

Vol 31 (5) ◽

pp. 383-388 ◽

Cited By ~ 2

Author(s):

Rahul Sharma ◽

Jitendra Kumar

Keyword(s):

Thin Layer ◽

Simultaneous Determination ◽

High Performance ◽

Chromatographic Method ◽

Development And Validation

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Development of a Purification Method for Simultaneous Determination of Deoxynivalenol and Its Acetylated and Glycosylated Derivatives in Corn Grits and Corn Flour by Liquid Chromatography–Tandem Mass Spectrometry

Journal of Food Protection ◽

10.4315/0362-028x.jfp-11-555 ◽

2012 ◽

Vol 75 (7) ◽

pp. 1355-1358 ◽

Cited By ~ 17

Author(s):

TOMOYA YOSHINARI ◽

TAKAHIRO OHNISHI ◽

TOMOYUKI KADOTA ◽

YOSHIKO SUGITA-KONISHI

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Tandem Mass Spectrometry ◽

Simultaneous Determination ◽

Tandem Mass ◽

Corn Flour ◽

Purification Method ◽

Chromatography Tandem Mass Spectrometry ◽

Liquid Chromatography Tandem Mass

We developed a purification method based on liquid chromatography–tandem mass spectrometry for the identification of deoxynivalenol (DON), its acetylated derivatives (3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol), and a glycosylated derivative (deoxynivalenol-3-glucoside [D3G]) in corn-based products. The analytes were extracted from samples with acetonitrile-water (85:15, vol/vol) and then purified with multifunctional columns. Evaluation of five kinds of multifunctional columns revealed that DON and its acetylated derivatives were recovered well (96 to 120%) by all columns, but D3G was recovered adequately (93.5%) by only one column, InertSep VRA-3. Samples of corn grits and corn flour were analyzed using the purification method with InertSep VRA-3. DON, D3G, and 15-acetyl-deoxynivalenol were the major contaminants in the samples harvested in 2009, but only DON was detected in the samples harvested in 2010. These results suggest that the purification method using InertSep VRA-3 is effective for identification of DON and its derivatives in corn-based products.

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