Rapid and sensitive identification of buffalo’s, cattle’s and sheep’s milk using species-specific PCR and PCR–RFLP techniques

Food Control ◽  
2007 ◽  
Vol 18 (10) ◽  
pp. 1246-1249 ◽  
Author(s):  
S.M. Abdel-Rahman ◽  
M.M.M. Ahmed
Keyword(s):  
Specific Pcr ◽  
Pcr Rflp ◽  
Species Specific

scholarly journals Efficiency of PCR-RFLP and species-specific PCR for the identification of meat origin in dry sausages

2017 ◽  
Vol 35 (No. 5) ◽  
pp. 386-391
Author(s):  
Kušec Ivona Djurkin ◽  
Samac Danijela ◽  
Margeta Vladimir ◽  
Radišić Žarko ◽  
Vincek Dragutin ◽  
...  
Keyword(s):  
Rflp Analysis ◽  
Chicken Meat ◽  
Specific Primers ◽  
Specific Pcr ◽  
Sheep Meat ◽  
Pcr Rflp ◽  
Pork Sausages ◽  
Species Specific ◽  
Dry Sausages

The purpose of this investigation was the identification of chicken, beef and sheep meat in pork sausages using PCR-RFLP and PCR with pecies-specific primers. Six dry fermented pork sausages were produced by adding beef, sheep and chicken meat to each in the amount of 1 and 5%. DNA was extracted from five regions of each sausage and PCR-RFLP together with PCR using species-specific primers was performed. PCR-RFLP analysis was successful only for chicken meat, while species-specific PCR was effective for identification of chicken, eef and sheep meat in all ratios and from all regions of the sausages. The results of our study show that discovering adulteration using PCR-RFLP is suitable only for chicken meat in the investigated products, while for detection of beef and sheep meat use of species-specific oligonucleotides is more effective.

Authentication of camel meat using species-specific PCR and PCR-RFLP

2020 ◽  
Author(s):  
S. Vaithiyanathan ◽  
M. R. Vishnuraj ◽  
G. Narender Reddy ◽  
Ch. Srinivas
Keyword(s):  
Specific Pcr ◽  
Pcr Rflp ◽  
Camel Meat ◽  
Species Specific

Molecular identification of zoonotic hookworm species in dog faeces from Tanzania

2018 ◽  
Vol 93 (3) ◽  
pp. 313-318 ◽  
Author(s):  
A. Merino-Tejedor ◽  
P. Nejsum ◽  
E.M. Mkupasi ◽  
M.V. Johansen ◽  
Annette Olsen
Keyword(s):  
Sanger Sequencing ◽  
Molecular Techniques ◽  
Health Concern ◽  
Specific Pcr ◽  
Molecular Approaches ◽  
Faecal Samples ◽  
Pcr Rflp ◽  
Veterinary Importance ◽  
Polymerase Chain ◽  
Species Specific

AbstractThe presence and distribution of various species of canine hookworms in Africa are poorly known. The main objective of this study, therefore, was to identify the hookworm species present in canine faecal samples from Morogoro, Tanzania, using molecular techniques. Faecal samples from 160 local dogs were collected and hookworm positive samples processed to recover larvae for further molecular characterization. DNA was extracted from pools of larvae from individual samples (n = 66), which were analysed subsequently using two different molecular approaches, polymerase chain reaction-linked restriction fragment length polymorphism (PCR-RFLP) and species-specific PCR coupled with Sanger sequencing. The PCR-RFLP technique detected only the presence of the ubiquitousAncylostoma caninumin the 66 samples. However, by species-specific PCR coupled with Sanger sequencing we identified ten samples withA. braziliense, two withUncinaria stenocephalaand five withA. ceylanicum. Thus, all four known species of canine hookworms were identified in Morogoro, Tanzania. To our knowledge this is the first report of the detection of the presence ofU. stenocephalaandA. ceylanicumin Africa using molecular techniques. In addition to their veterinary importance, canine hookworms have zoonotic potential and are of public health concern.

scholarly journals Identification of Meat Species Using Species-Specific PCR-RFLP Fingerprint of Mitochondrial 12S rRNA Gene

2007 ◽  
Vol 27 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Jong-Keun Park ◽  
Ki-Hyun Shin ◽  
Sung-Chul Shin ◽  
Ku-Young Chung ◽  
Eui-Ryong Chung
Keyword(s):  
12S Rrna ◽  
Rrna Gene ◽  
Specific Pcr ◽  
12S Rrna Gene ◽  
Pcr Rflp ◽  
Meat Species ◽  
Mitochondrial 12S Rrna ◽  
Species Specific

Molecular and morphological discrimination betweenTylospora fibrillosaandTylospora asterophoramycorrhizae

1999 ◽  
Vol 77 (1) ◽  
pp. 11-21 ◽  
Author(s):  
Ursula Eberhardt ◽  
Lutz Walter ◽  
Ingrid Kottke
Keyword(s):  
Fragment Length Polymorphism ◽  
Pcr Primers ◽  
Morphological Identification ◽  
Chain Reaction ◽  
Specific Primers ◽  
Specific Pcr ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Species Specific ◽  
First Time

Among the mycorrhizal types of spruce, Tylospora-type mycorrhizae are the most constant and abundant. Two species of the genus Tylospora occur in Europe, Tylospora fibrillosa and Tylospora asterophora. Mycorrhizae of T. asterophora are described in detail for the first time. Sequences of the internal transcribed spacer (ITS) of the ribosomal genes were obtained from T. fibrillosa and T. asterophora mycorrhizae, sporocarps, and cultured mycelium. Discrimination and identification of the two species by ITS polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) are discussed in the light of inter- and intra-specific variability. Species-specific PCR primers were designed to distinguish both species. Molecular screening of Tylospora-type mycorrhizae from field material led to unambiguous results, whereas morphological identification is likely to fail because of great similarity even at the microscopic level.Key words: Tylospora asterophora, Tylospora fibrillosa, ectomycorrhizae, taxon specific primers (TSOPs), ITS sequences.

Molecular identification of some Hoplolaimus species from the USA based on duplex PCR, multiplex PCR and PCR-RFLP analysis

Nematology ◽  
2009 ◽  
Vol 11 (3) ◽  
pp. 471-480 ◽  
Author(s):  
Robert Robbins ◽  
Allen Szalanski ◽  
Chang-Hwan Bae
Keyword(s):  
Multiplex Pcr ◽  
Dna Sequences ◽  
Rflp Analysis ◽  
Pcr Primers ◽  
Interspecific Variation ◽  
Molecular Techniques ◽  
Specific Pcr ◽  
Pcr Multiplex ◽  
Pcr Rflp ◽  
Species Specific

AbstractTwo different molecular approaches, a multiplex PCR and PCR-RFLP of ITS-rDNA, were developed for the identification of Hoplolaimus species. DNA sequences of H. columbus, H. galeatus, H. concaudajuvencus, H. magnistylus, H. seinhorsti and three undescribed Hoplolaimus species were used to design species-specific primers. Three reverse species-specific PCR primers for H. columbus, H. galeatus and H. magnistylus were developed using the ITS1 region exhibiting interspecific variation. Three species-specific PCR primers in combination with the forward primer, Hoc-1f, produced distinct amplicons of 580 bp for H. columbus, 120 bp for H. galeatus and 340 bp for H. magnistylus. We successfully identified each of three species by multiplex PCR when all three were mixed in a single PCR reaction. Restriction enzyme digests of the PCR amplicon using HaeIII and RsaI permitted discrimination of H. columbus, H. galeatus, H. magnistylus, H. concaudajuvencus, H. sp. 1, H. sp. 2 and H. sp. 3 from each other. These results suggest that these molecular techniques allow for rapid, easy and reliable identification of Hoplolaimus species.

Application of a species-specific PCR-RFLP to identify Ancylostoma eggs directly from canine faeces

2004 ◽  
Vol 123 (3-4) ◽  
pp. 245-255 ◽  
Author(s):  
Rebecca J. Traub ◽  
Ian D. Robertson ◽  
Peter Irwin ◽  
Norbert Mencke ◽  
R.C.Andrew Thompson
Keyword(s):  
Specific Pcr ◽  
Pcr Rflp ◽  
Species Specific

scholarly journals Associations Between Armillaria Species and Host Plants in U.K. Gardens

Plant Disease ◽  
2017 ◽  
Vol 101 (11) ◽  
pp. 1903-1909 ◽  
Author(s):  
Jassy Drakulic ◽  
Caroline Gorton ◽  
Ana Perez-Sierra ◽  
Gerard Clover ◽  
Liz Beal
Keyword(s):  
Host Plant ◽  
Plant Diversity ◽  
Root Rot ◽  
Host Plants ◽  
Specific Pcr ◽  
Royal Horticultural Society ◽  
Pcr Rflp ◽  
Species Specific ◽  
Armillaria Species

Honey fungus (Armillaria spp.) root rot is the disease most frequently inquired about by U.K. gardeners to the Royal Horticultural Society. Armillaria epidemiology has been studied within forestry and agriculture, but data are lacking within gardens, which have greater host plant diversity than orchards and vineyards and greater disturbance than woodlands. Which Armillaria species are responsible for garden disease, and how the broad range of susceptible ornamentals are differentially affected is not known. To address this, isolates of Armillaria were obtained from dead and dying plants from across the U.K. over a 4-year period (2004 to 2007). Species were identified by PCR-RFLP for IGS, and further verified by species-specific PCR for EF-1 α. Of the seven species known in the U.K., three were identified: A. mellea (83.1%), A. gallica (15.8%), and A. ostoyae (1.1%). Armillaria was isolated from trees, shrubs, and nonwoody plants including bulbs and vegetables, with newly recorded hosts listed herein. A. mellea was associated with infections of multiple hosts, and with all infections of the most common host, Ligustrum. In sites where more than one Armillaria species was found, the combination was of A. mellea and A. gallica, raising questions regarding the interactions of these species in U.K. gardens.

scholarly journals Molecular Identification ofAncylostoma caninumIsolated from Cats in Southern China Based on Complete ITS Sequence

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Yuanjia Liu ◽  
Guochao Zheng ◽  
Muhamd Alsarakibi ◽  
Xinheng Zhang ◽  
Wei Hu ◽  
...  
Keyword(s):  
Southern China ◽  
Sequence Similarity ◽  
Blood Feeding ◽  
Specific Pcr ◽  
Intestinal Nematode ◽  
Highly Sensitive ◽  
Main Host ◽  
Pcr Rflp ◽  
Species Specific ◽  
Single Cluster

Ancylostoma caninumis a blood-feeding parasitic intestinal nematode which infects dogs, cats, and other mammals throughout the world. A highly sensitive and species-specific PCR-RFLP technique was utilised to detect the prevalence ofA. caninumin cats in Guangzhou, southern China. Of the 102 fecal samples examined, the prevalence ofA. caninumin cats was 95.1% and 83.3% using PCR-RFLP and microscopy, respectively. Among them, the prevalence of single hookworm infection withA. caninumwas 54.90%, while mixed infections with bothA. caninumandA. ceylanicumwere 40.20%. Comparative analysis of three complete ITS sequences obtained from cat-derivedA. caninumshowed the same length (738 bp) as that of dog-derivedA. caninum. However, the sequence variation range was 98.6%–100%, where only one cat isolate (M63) showed 100% sequence similarity in comparison with two dog-derivedA. caninumisolates (AM850106, EU159416) in the same studied area. The phylogenetic tree revealedA. caninumderived from both cats and dogs in single cluster. Results suggest that cats could be the main host ofA. caninumin China, which may cause cross-infection between dogs and cats in the same area.

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